Name: Salibacterium nitratireducens SMB4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159796

Type strain:

Species: Salibacterium nitratireducens

Strain Designation: SMB4

Culture col. no.: MTCC 12633, KCTC 33876, JCM 32187

Strain history: <- P. Anil Kumar, Microbial Type Culture Collection and Gene Bank(MTCC), CSIR-IMTECH

NCBI tax ID(s): 399947 (species)

Section

Salibacterium nitratireducens SMB4 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from water sample from a salt lake.

colony-forming
Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Salibacterium*
	Species	**Salibacterium nitratireducens**
	Full Scientific Name (LPSN)	Salibacterium nitratireducens Singh et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66735]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #66735]	Cell length	1.1-1.3 µm

[Ref.: #66735]	Cell width	0.5-0.6 µm

[Ref.: #66735]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #66735]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #66735]	Colony size	1.2 mm
[Ref.: #66735]	Colony color	whitish
[Ref.: #66735]	Colony shape	circular
[Ref.: #66735]	Incubation period	1-2 days
[Ref.: #66735]	Cultivation medium used	Marine agar (MA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66735]

Culture medium

Marine agar (MA)

[Ref.: #66735]

Culture medium growth

		Temperature
[Ref.: #66735]	optimum	37 °C
[Ref.: #66735]	growth	25-50 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #66735]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66735]			optimum	7
[Ref.: #66735]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66735]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #66735]	Type of spore	endospore
[Ref.: #66735]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66735]		NaCl		growth	12	%(w/v)
[Ref.: #66735]		NaCl		optimum	10	%(w/v)

[Ref.: #66735]

Observation

The cell-wall peptidoglycan of strain SMB4T contains meso-diaminopimelic acid, while the polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66735]	arabinitol ChEBI	-	assimilation
[Ref.: #66735]	arabinose ChEBI	-	assimilation
[Ref.: #66735]	cellobiose ChEBI	-	assimilation
[Ref.: #66735]	D-arabinose ChEBI	-	assimilation
[Ref.: #66735]	erythritol ChEBI	-	assimilation
[Ref.: #66735]	esculin ChEBI	-	hydrolysis
[Ref.: #66735]	fructose ChEBI	-	assimilation
[Ref.: #66735]	galactitol ChEBI	-	assimilation
[Ref.: #66735]	galactose ChEBI	+	assimilation
[Ref.: #66735]	glucose ChEBI	+	assimilation
[Ref.: #66735]	glucose ChEBI	+	fermentation
[Ref.: #66735]	glycerol ChEBI	-	assimilation
[Ref.: #66735]	inulin ChEBI	-	assimilation
[Ref.: #66735]	isocitrate ChEBI	+	assimilation
[Ref.: #66735]	lactose ChEBI	-	assimilation
[Ref.: #66735]	malonate ChEBI	-	assimilation
[Ref.: #66735]	maltose ChEBI	+	assimilation
[Ref.: #66735]	mannitol ChEBI	+	assimilation
[Ref.: #66735]	mannose ChEBI	-	assimilation
[Ref.: #66735]	melibiose ChEBI	-	assimilation
[Ref.: #66735]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #66735]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #66735]	myo-inositol ChEBI	-	assimilation
[Ref.: #66735]	raffinose ChEBI	-	assimilation
[Ref.: #66735]	rhamnose ChEBI	-	assimilation
[Ref.: #66735]	ribitol ChEBI	-	assimilation
[Ref.: #66735]	salicin ChEBI	-	assimilation
[Ref.: #66735]	sodium gluconate ChEBI	-	assimilation
[Ref.: #66735]	sorbitol ChEBI	-	assimilation
[Ref.: #66735]	sorbose ChEBI	-	assimilation
[Ref.: #66735]	sucrose ChEBI	+	assimilation
[Ref.: #66735]	trehalose ChEBI	+	assimilation
[Ref.: #66735]	xylitol ChEBI	-	assimilation
[Ref.: #66735]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66735]	acetoin ChEBI	no
[Ref.: #66735]	hydrogen sulfide ChEBI	no
[Ref.: #66735]	indole-3-acetic acid ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #66735]		acetoin ChEBI	-
[Ref.: #66735]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #66735]	amylase	-
[Ref.: #66735]	beta-galactosidase	+	3.2.1.23
[Ref.: #66735]	caseinase	-	3.4.21.50
[Ref.: #66735]	catalase	+	1.11.1.6
[Ref.: #66735]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66735]	gelatinase	-
[Ref.: #66735]	lipase	-
[Ref.: #66735]	lysine decarboxylase	-	4.1.1.18
[Ref.: #66735]	nitrate reductase	+	1.7.99.4
[Ref.: #66735]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #66735]	tryptophan deaminase	-	4.1.99.1
[Ref.: #66735]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66735]

Fatty acid	Percentage
C15:0 anteiso	22.7
C17:0 anteiso	22.4
C18:0	10.6
C17:0 iso	8.1
C19:0 anteiso	4.8
C15:0 iso	4.7
C16:0	4.2
C19:0 iso	3.6
C16:0 iso	3.2
C20:0	2.2
C14:0 iso	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66735]	Sample type/isolated from	water sample from a salt lake
[Ref.: #66735]	Geographic location (country and/or sea, region)	Sambhar salt lake, Rajasthan
[Ref.: #66735]	Country	India
[Ref.: #66735]	Country ISO 3 Code	IND
[Ref.: #66735]	Continent	Asia
[Ref.: #66735]	Geographic location	26.9253°/75.1971°
[Ref.: #66735]	Enrichment culture	Zobell?s marine agar
[Ref.: #66735]	Enrichment culture duration	2 days
[Ref.: #66735]	Enrichment culture temperature	37 ̊C
[Ref.: #66735]	Isolation/enrichment procedure	the water sample was serially diluted (up to 10-fold dilutions) in 2% (w/v) NaCl solution and 100 µl of each dilution was spread-plated on Zobell's marine agar (MA; HiMedia) plates, and incubated at 37 C for 2 days. A number of different colony morphotypes were observed, one that appeared whitish in colour was selected and characterized.

[Ref.: #67770]	Sample type/isolated from	Water sample from Sambhar salt lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	Rajasthan
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From water sample, Sambhar salt lake
[Ref.: #67771]	Geographic location (country and/or sea, region)	Jaipur, Rajasthan
[Ref.: #67771]	Country	India
[Ref.: #67771]	Country ISO 3 Code	IND
[Ref.: #67771]	Continent	Asia
[Ref.: #67771]	Geographic location	26.9253°/75.1971°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT161881 (>99% sequence identity) for Salibacterium nitratireducens from Microbeatlas

Aquatic Samples	1
Soil Samples	1
Animal Samples	1
Plant Samples	1
Total Samples	4

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66735]	Bacillus sp. SMB4 partial 16S rRNA gene, strain SMB4, isolate MGM	LT161881	1516	ENA	399947 tax ID

[Ref.: #67771]

GC-content

49.1 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #66735]

Culture collection no.

MTCC 12633, KCTC 33876, JCM 32187

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Salibacterium nitratireducens sp. nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.	Singh H, Kaur M, Singh S, Mishra S, Kumar S, Vemuluri VR, Tanuku NRS, Pinnaka AK	Int J Syst Evol Microbiol	10.1099/ijsem.0.003021	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66735

Harjodh Singh, Manpreet Kaur, Shweta Singh, Sunita Mishra, Shekhar Kumar, Venkata Ramana Vemuluri, Naga Radha Srinivas Tanuku and Anil Kumar Pinnaka: Salibacterium nitratireducens sp. nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.. IJSEM 68: 3506 - 3511 2018 ( DOI 10.1099/ijsem.0.003021 , PubMed 30226463 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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