Name: Bacillus shivajii AK72
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159795

Type strain:

Species: Bacillus shivajii

Strain Designation: AK72

Culture col. no.: MTCC 12636, KCTC 33981, JCM 32183

Strain history: P. Anil Kumar; MTCC, India; AK72.

NCBI tax ID(s): 1983719 (species)

Section

Bacillus shivajii AK72 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from water sample.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus shivajii**
	Full Scientific Name (LPSN)	Bacillus shivajii Kumar et al. 2018

Synonym

"Evansella shivajii"

Information on morphological and physiological properties Morphology

[Ref.: #66723]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #66723]	Cell length	2.2-3.0 µm

[Ref.: #66723]	Cell width	0.3-0.4 µm

[Ref.: #66723]	Cell shape	rod-shaped

[Ref.: #66723]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.664

[Ref.: #66723]	Flagellum arrangement	polar

[Ref.: #66723]	Colony color	pale
[Ref.: #66723]	Colony shape	circular
[Ref.: #66723]	Incubation period	2 days
[Ref.: #66723]	Cultivation medium used	Marine agar (MA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66723]

Culture medium

Marine agar (MA)

[Ref.: #66723]

Culture medium growth

		Temperature
[Ref.: #66723]	growth	10-42 °C
[Ref.: #66723]	optimum	25-37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #66723]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66723]			optimum	9
[Ref.: #66723]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66723]

Oxygen tolerance

aerobe

[Ref.: #66723]	Spore description	a central and ellipsoidal spore
[Ref.: #66723]	Type of spore	endospore
[Ref.: #66723]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66723]		NaCl		growth	10	%(w/v)
[Ref.: #66723]		NaCl		optimum	3-5	%(w/v)

[Ref.: #66723]	Observation	The abundant polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminophospholipid.
[Ref.: #66723]	Observation	The cell-wall peptidoglycan contains meso-diaminopimelic acid.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66723]	agar ChEBI	-	hydrolysis
[Ref.: #66723]	arabinose ChEBI	-	assimilation
[Ref.: #66723]	casein	-	hydrolysis
[Ref.: #66723]	cellobiose ChEBI	-	assimilation
[Ref.: #66723]	cellulose ChEBI	-	hydrolysis
[Ref.: #66723]	dna ChEBI	-	hydrolysis
[Ref.: #66723]	esculin ChEBI	+	hydrolysis
[Ref.: #66723]	gelatin ChEBI	-	hydrolysis
[Ref.: #66723]	glucose ChEBI	+	assimilation
[Ref.: #66723]	glucose ChEBI	-	fermentation
[Ref.: #66723]	isocitrate ChEBI	+	assimilation
[Ref.: #66723]	lactose ChEBI	-	assimilation
[Ref.: #66723]	malonate ChEBI	-	assimilation
[Ref.: #66723]	maltose ChEBI	-	assimilation
[Ref.: #66723]	mannitol ChEBI	+	assimilation
[Ref.: #66723]	melibiose ChEBI	-	assimilation
[Ref.: #66723]	nitrate ChEBI	+	reduction
[Ref.: #66723]	raffinose ChEBI	-	assimilation
[Ref.: #66723]	rhamnose ChEBI	-	assimilation
[Ref.: #66723]	ribitol ChEBI	-	assimilation
[Ref.: #66723]	starch ChEBI	-	hydrolysis
[Ref.: #66723]	sucrose ChEBI	-	assimilation
[Ref.: #66723]	trehalose ChEBI	+	assimilation
[Ref.: #66723]	tween 20 ChEBI	+	hydrolysis
[Ref.: #66723]	tween 40 ChEBI	+	hydrolysis
[Ref.: #66723]	tween 60 ChEBI	+	hydrolysis
[Ref.: #66723]	tween 80 ChEBI	+	hydrolysis
[Ref.: #66723]	urea ChEBI	+	hydrolysis
[Ref.: #66723]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66723]	amoxicillin ChEBI				yes	30	µg (disc)
[Ref.: #66723]	bacitracin ChEBI	yes	8	Unit (disc)
[Ref.: #66723]	cefadroxil ChEBI	yes	30	µg (disc)
[Ref.: #66723]	cefazolin ChEBI	yes	30	µg (disc)
[Ref.: #66723]	cefprozil ChEBI	yes	30	µg (disc)
[Ref.: #66723]	ceftazidime ChEBI				yes	30	µg (disc)
[Ref.: #66723]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #66723]	chlortetracyclin ChEBI	yes	30	µg (disc)
[Ref.: #66723]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #66723]	penicillin ChEBI				yes	2	Unit (disc)
[Ref.: #66723]	streptomycin ChEBI	yes	10	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66723]	acetoin ChEBI	no
[Ref.: #66723]	hydrogen sulfide ChEBI	no
[Ref.: #66723]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #66723]		acetoin ChEBI	-
[Ref.: #66723]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #66723]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66723]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66723]	arginine dihydrolase	+	3.5.3.6
[Ref.: #66723]	beta-galactosidase	-	3.2.1.23
[Ref.: #66723]	catalase	+	1.11.1.6
[Ref.: #66723]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66723]	leucine arylamidase	+	3.4.11.1
[Ref.: #66723]	lysine decarboxylase	-	4.1.1.18
[Ref.: #66723]	nitrate reductase	+	1.7.99.4
[Ref.: #66723]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #66723]	phenylalanine arylamidase	+
[Ref.: #66723]	phenylalanine deaminase	+	4.3.1.5
[Ref.: #66723]	proline-arylamidase	+	3.4.11.5
[Ref.: #66723]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #66723]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66723]

Fatty acid	Percentage
C15:0 anteiso	26.2
C15:0 iso	17.4
C16:0 iso	12.4
C14:0 iso	8.9
C17:0 anteiso	4.9
C17:1 iso w5c	4.2
C16:0	2.7
C17:0 iso	2.5
C14:0	1.1
C18:0	0.5
C12:0	0.5
C18:0 iso	0.2
C13:0 iso	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66723]	Sample type/isolated from	water sample
[Ref.: #66723]	Geographic location (country and/or sea, region)	Sambhar salt lake, Rajasthan
[Ref.: #66723]	Country	India
[Ref.: #66723]	Country ISO 3 Code	IND
[Ref.: #66723]	Continent	Asia
[Ref.: #66723]	Enrichment culture	Zobell?s marine agar
[Ref.: #66723]	Enrichment culture duration	2 days
[Ref.: #66723]	Enrichment culture temperature	37 ̊C
[Ref.: #66723]	Isolation/enrichment procedure	the water sample was serially diluted (up to 10-fold dilutions) in 2% (w/v) NaCl solution and 100 µl of each dilution was spread-plated on Zobell's marine agar (MA; HiMedia) plates and incubated at 37 C for 2 days. Out of the different colony morphotypes observed, one translucent, pale colony was selected and purified.

[Ref.: #67770]	Sample type/isolated from	Water sample from Sambhar salt lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	Rajasthan
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66723]	Bacillus sp. AK72 partial 16S rRNA gene, strain AK72, isolate SK70A	LT844662	1458	ENA	1983719 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus shivajii JCM 32183	GCA_020519665	complete	GenBank	1983719 tax ID	*
[Ref.: #66792]	Bacillus shivajii strain JCM 32183	1983719.5	complete	PATRIC	1983719 tax ID	*
[Ref.: #66792]	Bacillus shivajii strain JCM 32183	1983719.3	complete	PATRIC	1983719 tax ID	*
[Ref.: #66792]	Bacillus shivajii strain JCM 32183	1983719.4	complete	PATRIC	1983719 tax ID	*

[Ref.: #67770]

GC-content

36.8 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus shivajii JCM 32183 NCBI: GCA_020519665.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus shivajii JCM 32183 NCBI: GCA_020519665.1
[Ref.: #69480]	flagellated	yes	85.85	yes
[Ref.: #69480]	gram-positive	yes	87.149	yes
[Ref.: #69480]	anaerobic	no	96.63	yes
[Ref.: #69480]	aerobic	yes	84.382	yes
[Ref.: #69480]	halophile	yes	83.533	yes
[Ref.: #69480]	spore-forming	yes	94.761	no
[Ref.: #69480]	motile	yes	87.317	yes
[Ref.: #69480]	thermophile	no	91.748	yes
[Ref.: #69480]	glucose-util	yes	84.835	no
[Ref.: #69480]	glucose-ferment	no	91.918	no

Availability in culture collections External links

[Ref.: #66723]

Culture collection no.

MTCC 12636, KCTC 33981, JCM 32183

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus shivajii sp. nov., isolated from a water sample of Sambhar salt lake, India.	Kumar S, Singh H, Kaur M, Kaur L, Tanuku NRS, Pinnaka AK	Int J Syst Evol Microbiol	10.1099/ijsem.0.003008	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66723

Shekhar Kumar, Harjodh Singh, Manpreet Kaur, Lakhwinder Kaur, Naga Radha Srinivas Tanuku and Anil Kumar Pinnaka: Bacillus shivajii sp. nov., isolated from a water sample of Sambhar salt lake, India. IJSEM 68: 3463 - 3470 2018 ( DOI 10.1099/ijsem.0.003008 , PubMed 30207517 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Bacillus shivajii in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Bacillus shivajii JCM 32183

Bacillus shivajii JCM 32183

Change proposal

Change proposal