[Ref.: #66640] |
Sample type/isolated from |
cultured in situ sediment sample |
[Ref.: #66640] |
Geographic location (country and/or sea, region) |
coast in the East China Sea |
[Ref.: #66640] |
Country |
China |
[Ref.: #66640] |
Country ISO 3 Code |
CHN |
[Ref.: #66640] |
Continent |
Asia |
[Ref.: #66640] |
Geographic location |
29.9333°/122.0830° |
[Ref.: #66640] |
Enrichment culture |
modified marine agar 2216 |
[Ref.: #66640] |
Enrichment culture composition |
replaced Na2SO4 with 6.64 g MgSO4 x 7H2O and decreased the amount of MgCl2 x 6H2O to 12.6 g. |
[Ref.: #66640] |
Enrichment culture duration |
15 days |
[Ref.: #66640] |
Enrichment culture temperature |
20 ̊C |
[Ref.: #66640] |
Isolation/enrichment procedure |
We used YTP medium, which contained (per litre distilled water): 20 g NaCl, 0.5 g yeast extract, 1.0 g tryptone, 10 g peel of pemelo (Citrus maxima) and 20 g agar, pH 7.0. YTP agar medium was made and divided into 50 ml PP tubes sealed by gauze. After autoclaving, the gauze was replaced by single layer aseptic linen, and the tubes were placed into autoclaved barrels aseptically. We then buried this device on a coast in the East China Sea and dug it out after 1 year of culturing. For subsequent experiments, 1cm-thick medium on the upper layer was cut off. To isolate the strain, the standard dilution plating technique on modified marine agar 2216. |
|
[Ref.: #67770] |
Sample type/isolated from |
Cultured in situ sediment sample collected on the coast of the East China Sea |
|
[Ref.: #67771] |
Sample type/isolated from |
From marine sediment in the East China Sea |
[Ref.: #67771] |
Geographic location (country and/or sea, region) |
Zhejiang Province |
[Ref.: #67771] |
Country |
China |
[Ref.: #67771] |
Country ISO 3 Code |
CHN |
[Ref.: #67771] |
Continent |
Asia |
* marker position based on {}
|
|
Isolation sources categories |
#Engineered |
#Laboratory |
#Lab enrichment |
#Environmental |
#Terrestrial |
#Sandy |
|
|
- References
-
-
-
-
#67770 |
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
|
-
#67771 |
Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
|
-
- * These data were automatically processed and therefore are not curated
Change proposal
Successfully sent
|