Name: Zhengella mangrovi X9-2-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159728

Type strain:

Species: Zhengella mangrovi

Strain Designation: X9-2-2

Culture col. no.: MCCC 1K03307, JCM 32107

Strain history: T. Yun; Xiamen Univ., China; X9-2-2.

NCBI tax ID(s): 1982044 (species)

Section

Zhengella mangrovi X9-2-2 is a facultative anaerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from mangrove sediment sample.

colony-forming
Gram-negative
motile
rod-shaped
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Notoacmeibacteraceae*
	Genus	*Zhengella*
	Species	**Zhengella mangrovi**
	Full Scientific Name (LPSN)	Zhengella mangrovi Liao et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66636]	Gram stain	negative

[Ref.: #66636]	Cell length	1.5-3 µm

[Ref.: #66636]	Cell width	0.8-1.1 µm

[Ref.: #66636]	Cell shape	rod-shaped

[Ref.: #66636]	Motility	yes

[Ref.: #66636]	Flagellum arrangement	polar

[Ref.: #66636]	Colony size	0.5-2 mm
[Ref.: #66636]	Colony color	white
[Ref.: #66636]	Colony shape	circular
[Ref.: #66636]	Incubation period	3 days
[Ref.: #66636]	Cultivation medium used	Zobell 2216 E agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66636]

Culture medium

Zobell 2216 E agar

[Ref.: #66636]

Culture medium growth

		Temperature
[Ref.: #66636]	growth	15-40 °C
[Ref.: #66636]	optimum	30-37 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #66636]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66636]			optimum	7
[Ref.: #66636]			growth	4-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66636]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66636]		NaCl		growth	0.5-7	%(w/v)
[Ref.: #66636]		NaCl		optimum	2	%(w/v)

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66636]	adipate ChEBI	+	assimilation
[Ref.: #66636]	amygdalin ChEBI	+	builds acid from
[Ref.: #66636]	arabinose ChEBI	-	builds acid from
[Ref.: #66636]	casein	-	hydrolysis
[Ref.: #66636]	casein hydrolysate	-	hydrolysis
[Ref.: #66636]	chitin ChEBI	-	hydrolysis
[Ref.: #66636]	citric acid ChEBI	+	assimilation
[Ref.: #66636]	D-glucose ChEBI	+	assimilation
[Ref.: #66636]	D-glucose ChEBI	-	fermentation
[Ref.: #66636]	D-mannitol ChEBI	+	assimilation
[Ref.: #66636]	D-mannose ChEBI	-	assimilation
[Ref.: #66636]	decanoate ChEBI	-	assimilation
[Ref.: #66636]	gelatin ChEBI	+	hydrolysis
[Ref.: #66636]	glucose ChEBI	+	builds acid from
[Ref.: #66636]	L-arabinose ChEBI	+	assimilation
[Ref.: #66636]	malate ChEBI	+	assimilation
[Ref.: #66636]	maltose ChEBI	+	assimilation
[Ref.: #66636]	mannitol ChEBI	+	builds acid from
[Ref.: #66636]	melibiose ChEBI	-	builds acid from
[Ref.: #66636]	myo-inositol ChEBI	+	builds acid from
[Ref.: #66636]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66636]	nitrate ChEBI	+	reduction
[Ref.: #66636]	phenylacetate ChEBI	+	assimilation
[Ref.: #66636]	potassium gluconate ChEBI	+	assimilation
[Ref.: #66636]	rhamnose ChEBI	-	builds acid from
[Ref.: #66636]	sodium citrate ChEBI	-	assimilation
[Ref.: #66636]	sorbitol ChEBI	-	builds acid from
[Ref.: #66636]	starch ChEBI	-	hydrolysis
[Ref.: #66636]	sucrose ChEBI	+	builds acid from
[Ref.: #66636]	tween 20 ChEBI	+	hydrolysis
[Ref.: #66636]	tween 40 ChEBI	-	hydrolysis
[Ref.: #66636]	tween 60 ChEBI	-	hydrolysis
[Ref.: #66636]	tween 80 ChEBI	-	hydrolysis
[Ref.: #66636]	xylose ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66636]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #66636]	cefazolin ChEBI	yes	30	µg (disc)
[Ref.: #66636]	cephalexin ChEBI	yes	30	µg (disc)
[Ref.: #66636]	cephradine ChEBI				yes	30	µg (disc)
[Ref.: #66636]	cloxacillin ChEBI	yes	1	µg (disc)
[Ref.: #66636]	colistin ChEBI				yes	10	µg (disc)
[Ref.: #66636]	doxycycline ChEBI	yes	30	µg (disc)
[Ref.: #66636]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #66636]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #66636]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #66636]	minocycline ChEBI	yes	30	µg (disc)
[Ref.: #66636]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #66636]	norfloxacin ChEBI	yes	10	µg (disc)
[Ref.: #66636]	novobiocin ChEBI	yes	5	µg (disc)
[Ref.: #66636]	ofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #66636]	penicillin g ChEBI	yes	10	Unit (disc)
[Ref.: #66636]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #66636]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #66636]	sulfisoxazole ChEBI	yes	300	µg (disc)
[Ref.: #66636]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #66636]	trimethoprim ChEBI				yes	5	µg (disc)
[Ref.: #66636]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66636]	acetoin ChEBI	yes
[Ref.: #66636]	hydrogen sulfide ChEBI	no
[Ref.: #66636]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #66636]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #66636]	acid phosphatase	+	3.1.3.2
[Ref.: #66636]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66636]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66636]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66636]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66636]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66636]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66636]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66636]	beta-galactosidase	-	3.2.1.23
[Ref.: #66636]	beta-glucosidase	-	3.2.1.21
[Ref.: #66636]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66636]	catalase	+	1.11.1.6
[Ref.: #66636]	cystine arylamidase	+	3.4.11.3
[Ref.: #66636]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66636]	esterase (C 4)	+
[Ref.: #66636]	esterase Lipase (C 8)	+
[Ref.: #66636]	gelatinase	-
[Ref.: #66636]	leucine arylamidase	+	3.4.11.1
[Ref.: #66636]	lipase (C 14)	-
[Ref.: #66636]	lysine decarboxylase	-	4.1.1.18
[Ref.: #66636]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66636]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66636]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #66636]	trypsin	-	3.4.21.4
[Ref.: #66636]	tryptophan deaminase	-	4.1.99.1
[Ref.: #66636]	urease	+	3.5.1.5
[Ref.: #66636]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66636]

Fatty acid	Percentage
C18:1 w7c and/or C18:1 w6c	56
C17:0 iso	9.1
C12:0	6.6
C15:1-iso G	3.3
C11:1 2OH	2.9
C17:1 w8c	2.2
C16:0	2.2
C17:0	2
C18:0	1.9
C15:1 anteiso A	1.8
C16:1 w7c and/or C16:1 w6c	1.3
C17:1 iso w9c / C16:0 10-methyl	1.2
C18:1 w7c 11-methyl	1.2
C17:0 -cyclo	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66636]	Sample type/isolated from	mangrove sediment sample
[Ref.: #66636]	Geographic location (country and/or sea, region)	Yunxiao Mangrove National Nature Reserve, Zhangjiang Estuary
[Ref.: #66636]	Country	China
[Ref.: #66636]	Country ISO 3 Code	CHN
[Ref.: #66636]	Continent	Asia
[Ref.: #66636]	Geographic location	24.8666°/117.5830°
[Ref.: #66636]	Enrichment culture	Zobell 2216 E agar
[Ref.: #66636]	Enrichment culture composition	(5 g peptone, 1 g yeast extract, 0.0744 g KH2PO4, 0.002 g Fe2(SO4)3, 12 g agar, 750 ml aged seawater, 250 ml distilled water; pH 7.0-7.2)
[Ref.: #66636]	Enrichment culture duration	7 days
[Ref.: #66636]	Enrichment culture temperature	30 ̊C
[Ref.: #66636]	Isolation/enrichment procedure	The sediment sample was enriched with benzo[a]pyrene (10 ppm) for 6 months, and a serial dilution of 10-2, 10-3 and 10-4 were selected and 100 µl portion was spread on Zobell 2216 E agar. The plates were aerobically incubated in the dark at 30°C for 7 days.

[Ref.: #67770]	Sample type/isolated from	Mangrove sediment collected from Yunxiao Mangrove National Nature Reserve (24° 52' N 117° 35' E), located at the Zhangjiang Estuary in Southeast China
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Mangrove
#Environmental	#Terrestrial	#Sediment
#Host	#Plants	#Tree

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66636]	Phyllobacteriaceae bacterium X9-2-2 16S ribosomal RNA gene, partial sequence	MF034047	1445	ENA	1982044 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Zhengella mangrovi X9-2-2	GCA_002727065	contig	GenBank	1982044 tax ID
[Ref.: #66792]	Zhengella mangrovi X9-2-2	2831836058	draft	JGI IMG/M	1982044 tax ID	*

[Ref.: #66636]

GC-content

64.9 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #66636]

Culture collection no.

MCCC 1K03307, JCM 32107

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Salaquimonas pukyongi gen. nov., sp. nov., a novel bacterium within the family Phyllobacteriaceae.	Kim YS, Jeon YJ, Kim KH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003659	2019	*
Phylogeny	Zhengella mangrovi gen. nov., sp. nov., a novel member of family Phyllobacteriaceae isolated from mangrove sediment.	Liao H, Li Y, Lin X, Lai Q, Tian Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.002900	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66636

Hu Liao, Yuqian Li, Xiaolan Lin, Qiliang Lai, Yun Tian: Zhengella mangrovi gen. nov., sp. nov., a novel member of family Phyllobacteriaceae isolated from mangrove sediment. IJSEM 68: 2819 - 2825 2018 ( DOI 10.1099/ijsem.0.002900 , PubMed 30028281 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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