Name: Anaerosphaera multitolerans GS7-6-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159365

Type strain:

Species: Anaerosphaera multitolerans

Strain Designation: GS7-6-2

Culture col. no.: DSM 107952, CECT 9705

Strain history: <- R. Rettenmaier, TU Munich, Chair of Microbiology, Freising-Weihenstephan, Germany; <- R. Rettenmaier; {2017}

NCBI tax ID(s): 2487351 (species)

SI-ID 401178

DSM 107952

Section

Anaerosphaera multitolerans GS7-6-2 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from mesophilically operated lab scale biogas plant fed with maize silage.

Gram-positive
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptoniphilaceae*
	Genus	*Anaerosphaera*
	Species	**Anaerosphaera multitolerans**
	Full Scientific Name (LPSN)	Anaerosphaera multitolerans Rettenmaier et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66188]

Culture medium

CLOSTRIDIUM (GS) MEDIUM (DSMZ Medium 255)

[Ref.: #66188]

Culture medium growth

[Ref.: #66188]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66188]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #66188]			growth	30 °C

[Ref.: #66188]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Oxygen tolerance

anaerobe Confidence in %100

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.981
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66188]	Sample type/isolated from	mesophilically operated lab scale biogas plant fed with maize silage
[Ref.: #66188]	Geographic location (country and/or sea, region)	Freising
[Ref.: #66188]	Country	Germany
[Ref.: #66188]	Country ISO 3 Code	DEU
[Ref.: #66188]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Shrub (Scrub)
#Engineered	#Bioreactor	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MK138668 (>99% sequence identity) for Anaerosphaera multitolerans subclade from Microbeatlas

Aquatic Samples	42
Soil Samples	34
Animal Samples	382
Plant Samples	39
Total Samples	497

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66188]	Anaerosphaera multitolerans strain GS7-6-2 16S ribosomal RNA gene, partial sequence	MK138668	1422	ENA	2487351 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Anaerosphaera multitolerans GS7-6-2	GCA_004006535	contig	GenBank	2487351 tax ID	*
[Ref.: #66792]	Anaerosphaera sp. GS7-6-2	2487351.3	wgs	PATRIC	2487351 tax ID	*
[Ref.: #66792]	Anaerosphaera multitolerans GS7-6-2	2880949579	draft	JGI IMG/M	2487351 tax ID	*

[Ref.: #66188]

GC-content

32.8 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Anaerosphaera sp. GS7-6-2 PATRIC: 2487351.3
[Ref.: #69481]	spore-forming	yes	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Anaerosphaera sp. GS7-6-2 PATRIC: 2487351.3
[Ref.: #69480]	motile	no	91.576	no
[Ref.: #69480]	gram-positive	yes	91.212	no
[Ref.: #69480]	anaerobic	yes	98.333	no
[Ref.: #69480]	aerobic	no	97.378	no
[Ref.: #69480]	halophile	no	73.643	no
[Ref.: #69480]	spore-forming	no	84.797	no
[Ref.: #69480]	glucose-util	yes	84.618	no
[Ref.: #69480]	flagellated	no	96.976	no
[Ref.: #69480]	thermophile	no	96.877	yes
[Ref.: #69480]	glucose-ferment	no	54.24	no

Availability in culture collections External links

[Ref.: #66188]

Culture collection no.

DSM 107952, CECT 9705

[Ref.: #111860]

SI-ID 401178

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Anaerosphaera multitolerans sp. nov., a salt-tolerant member of the family Peptoniphilaceae isolated from a mesophilically operated biogas fermenter fed with maize silage.	Rettenmaier R, Liebl W, Zverlov VV	Int J Syst Evol Microbiol	10.1099/ijsem.0.003903	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66188

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107952

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111860

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401178.1 )

* These data were automatically processed and therefore are not curated

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Anaerosphaera sp. GS7-6-2

Anaerosphaera sp. GS7-6-2

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