Pinisolibacter ravus (KEMB 9005-534, KACC 19120, NBRC 112686)

Name: Pinisolibacter ravus (KEMB 9005-534, KACC 19120, NBRC 112686)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158823

Type strain

Strain Designation E9

Culture col. no. KEMB 9005-534 KACC 19120 NBRC 112686 E9

NCBI tax ID(s) 2172023

Special Collections Literature strains

Links

Pinisolibacter ravus E9 is a facultative anaerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from pine forest soil.

Gram-negative rod-shaped colony-forming facultative anaerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 394396

NBRC 112686

@ref 20215

Last LPSN update 2026-05-29 08:44:03

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Ancalomicrobiaceae

Genus Pinisolibacter

Species Pinisolibacter ravus

Full scientific name Pinisolibacter ravus Dahal et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
65587	negative	2.4-3.6 µm	0.9-1.8 µm	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
65587	1-2 mm	straw	circular	4 days	R2A

Pigmentation

@ref	Production	Name
65587		flexirubin type pigments
65587		carotenoid pigment

Culture and growth conditions

Culture medium

@ref	Name	Growth
65587	veal infusion
65587	Reasoner's 2A agar (R2A)
65587	Trypticase Soy Agar (TSA)
65587	Nutrient agar (NA)
65587	Marine agar (MA)
65587	LBA
65587	BHI
65587	PDA

Culture temp

@ref	Growth	Type	Temperature (°C)
65587	positive	growth	15-45
65587	positive	optimum	28-32

Culture pH

@ref	Ability	Type	PH	PH range
65587	positive	optimum	6.5-8.5
65587	positive	growth	5.0-10.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

65587

Oxygen tolerancefacultative anaerobe

Spore formation

65587

Spore formationno

Halophily

@ref	Halophily level	Salt	Growth	Tested relation	Concentration
65587		NaCl	positive	growth	0-0.2 %
65587	non-halophilic	NaCl	positive	optimum	0 %

Observation

65587

Observationprosthecae

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65587	16808 ChEBI	2-dehydro-D-gluconate	-	assimilation
65587	16193 ChEBI	3-hydroxybenzoate	-	assimilation
65587	37054 ChEBI	3-hydroxybutyrate	+	assimilation
65587	17879 ChEBI	4-hydroxybenzoate	-	assimilation
65587	58143 ChEBI	5-dehydro-D-gluconate	-	assimilation
65587	17128 ChEBI	adipate	-	assimilation
65587	85146 ChEBI	carboxymethylcellulose	-	hydrolysis
65587		casein	-	hydrolysis
65587	17029 ChEBI	chitin	-	hydrolysis
65587	17634 ChEBI	D-glucose	+	assimilation
65587	16899 ChEBI	D-mannitol	-	assimilation
65587	16024 ChEBI	D-mannose	-	assimilation
65587	16988 ChEBI	D-ribose	-	assimilation
65587	17924 ChEBI	D-sorbitol	-	assimilation
65587	27689 ChEBI	decanoate	-	assimilation
65587	16991 ChEBI	dna	-	hydrolysis
65587		esculin ferric citrate	+	hydrolysis
65587	5291 ChEBI	gelatin	-	hydrolysis
65587	17234 ChEBI	glucose	+	fermentation
65587	28087 ChEBI	glycogen	-	assimilation
65587	17368 ChEBI	hypoxanthine	-	hydrolysis
65587	16977 ChEBI	L-alanine	-	assimilation
65587	30849 ChEBI	L-arabinose	-	assimilation
65587	30849 ChEBI	L-arabinose	+	assimilation
65587	18287 ChEBI	L-fucose	-	assimilation
65587	17203 ChEBI	L-proline	+	assimilation
65587	62345 ChEBI	L-rhamnose	-	assimilation
65587	24996 ChEBI	lactate	-	assimilation
65587	25115 ChEBI	malate	-	assimilation
65587	17306 ChEBI	maltose	-	assimilation
65587	28053 ChEBI	melibiose	-	assimilation
65587	17268 ChEBI	myo-inositol	-	assimilation
65587	506227 ChEBI	N-acetylglucosamine	-	assimilation
65587	17632 ChEBI	nitrate	-	reduction
65587	17814 ChEBI	salicin	-	assimilation
65587	32954 ChEBI	sodium acetate	-	assimilation
65587	53258 ChEBI	sodium citrate	-	assimilation
65587	28017 ChEBI	starch	-	hydrolysis
65587	17992 ChEBI	sucrose	+	assimilation
65587	27897 ChEBI	tryptophan	-	energy source
65587	53423 ChEBI	tween 40	-	hydrolysis
65587	53425 ChEBI	tween 60	-	hydrolysis
65587	53426 ChEBI	tween 80	-	hydrolysis
65587	18186 ChEBI	tyrosine	-	hydrolysis
65587	31011 ChEBI	valerate	+	assimilation
65587	15318 ChEBI	xanthine	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite
65587	15688 ChEBI	acetoin
65587	16136 ChEBI	hydrogen sulfide
65587	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
65587	15688 ChEBI	acetoin	-
65587	35581 ChEBI	indole		-

Enzymes

@ref	Value	Activity	Ec
65587	acid phosphatase	+	3.1.3.2
65587	alkaline phosphatase	+	3.1.3.1
65587	alpha-chymotrypsin	-	3.4.21.1
65587	alpha-fucosidase	-	3.2.1.51
65587	alpha-galactosidase	-	3.2.1.22
65587	alpha-glucosidase	+	3.2.1.20
65587	alpha-mannosidase	-	3.2.1.24
65587	beta-galactosidase	-	3.2.1.23
65587	beta-glucosidase	+	3.2.1.21
65587	beta-glucuronidase	-	3.2.1.31
65587	catalase	-	1.11.1.6
65587	cystine arylamidase	+/-	3.4.11.3
65587	cytochrome oxidase	+	1.9.3.1
65587	esterase (C 4)	+/-
65587	esterase Lipase (C 8)	+
65587	leucine arylamidase	+	3.4.11.1
65587	lipase (C 14)	-
65587	N-acetyl-beta-glucosaminidase	-	3.2.1.52
65587	naphthol-AS-BI-phosphohydrolase	+
65587	trypsin	+	3.4.21.4
65587	tryptophan deaminase	-	4.1.99.1
65587	urease	+	3.5.1.5
65587	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

65587

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	52.3
C16:1ω7c / C16:1ω6c	25.4
C16:0	11.5
C12:0 aldehyde / unknown 10.928	5.5
C18:0	2
C18:0 3OH	1
C20:1ω7c	0.9
C14:0	0.7
C19:1ω6c / C19:0 cyclo	0.4
C20:0	0.3

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
65587	pine forest soil	Kyonggi University, Suwon, Gyeonggi-Do	Republic of Korea	KOR	Asia	R2A	14 days	28	six-transwell plates with 3 ml R2A broth and 3 g sample of sieved soil. 1 ml of a soil suspension (1g of soil in 9 ml R2A broth) was added.

Taxonmaps

69479

Global distribution of 16S sequence KY087994 (>99% sequence identity) for Pinisolibacter ravus subclade from Microbeatlas

Aquatic Samples	4656
Soil Samples	839
Animal Samples	875
Plant Samples	963
Total Samples	7333

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
65587	Pinisolibacter ravus strain E9 16S ribosomal RNA gene, partial sequence	KY087994

GC content

@ref	GC-content (mol%)	Method
65587	68.4	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pinisolibacter ravus gen. nov., sp. nov., isolated from pine forest soil and allocation of the genera Ancalomicrobium and Pinisolibacter to the family Ancalomicrobiaceae fam. nov., and emendation of the genus Ancalomicrobium Staley 1968.	Dahal RH, Chaudhary DK, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002772	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65587	Ram Hari Dahal, Dhiraj Kumar Chaudhary and Jaisoo Kim: Pinisolibacter ravus gen. nov., sp. nov., isolated from pine forest soil and allocation of the genera Ancalomicrobium and Pinisolibacter to the family Ancalomicrobiaceae fam. nov., and emendation of the genus Ancalomicrobium Staley 1968. IJSEM 68: 1955 - 1962 2018 ( DOI 10.1099/ijsem.0.002772 , PubMed 29683414 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pinisolibacter ravus (KEMB 9005-534, KACC 19120, NBRC 112686)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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