Name: Flavobacterium phocarum SE14
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158683

Type strain:

Species: Flavobacterium phocarum

Strain Designation: SE14

Culture col. no.: CCTCC AB 2017225, KCTC 52612

Strain history: <- Zongjun Du, Shandong Univ.

NCBI tax ID(s): 2065192 (species)

Special Collections

Section

Flavobacterium phocarum SE14 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from From soil in a seal habitat on King George Island.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium phocarum**
	Full Scientific Name (LPSN)	Flavobacterium phocarum Zhou et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65419]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #65419]	Cell length	0.9-4.9 µm

[Ref.: #65419]	Cell width	0.3-0.6 µm

[Ref.: #65419]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #65419]	Motility	no

[Ref.: #65419]	Colony size	1-2.55 mm
[Ref.: #65419]	Colony color	yellow
[Ref.: #65419]	Colony shape	circular
[Ref.: #65419]	Incubation period	4 days
[Ref.: #65419]	Cultivation medium used	on Anacker and Ordal agar, marine agar, R2A and well on egg yolk agar, nutrient agar, tryptic soy agar and sea-salt-free Zobell's agar, not on MacCon-key agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65419]

Culture medium

on Anacker and Ordal agar, marine agar, R2A and well on egg yolk agar, nutrient agar, tryptic soy agar and sea-salt-free Zobell's agar, not on MacCon-key agar

[Ref.: #65419]

Culture medium growth

		Temperature
[Ref.: #65419]	growth	4-25 °C
[Ref.: #65419]	optimum	20 °C
[Ref.: #67771]	growth	20 °C

	pH	Kind of pH		pH
[Ref.: #65419]			optimum	7-7.5
[Ref.: #65419]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65419]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65419]		NaCl		growth	0-3	%
[Ref.: #65419]		NaCl		optimum	1-1.5	%

[Ref.: #65419]	Observation	grows weakly under microaerobic conditions (with 5 % O2 , 10 % CO2 and 85 % N2), poorly under anaerobic conditions (with 10 % H2 , 10 % CO2 and 80 % N2)
[Ref.: #65419]	Observation	only respiratory quinone is menaquinone-6
[Ref.: #65419]	Observation	major polar lipids are phosphatidylethanolamine and four unidentified aminolipids
[Ref.: #67771]	Observation	quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65419]	3-O-methyl alpha-D-glucopyranoside	-	builds acid from
[Ref.: #65419]	alginate ChEBI	-	assimilation
[Ref.: #65419]	amygdalin ChEBI	-	builds acid from
[Ref.: #65419]	arbutin ChEBI	-	builds acid from
[Ref.: #65419]	casein	+	assimilation
[Ref.: #65419]	cellobiose ChEBI	-	builds acid from
[Ref.: #65419]	cellulose ChEBI	-	assimilation
[Ref.: #65419]	chitin ChEBI	-	assimilation
[Ref.: #65419]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65419]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65419]	D-fructose ChEBI	-	builds acid from
[Ref.: #65419]	D-fucose ChEBI	-	builds acid from
[Ref.: #65419]	D-galactose ChEBI	-	builds acid from
[Ref.: #65419]	D-glucose ChEBI	-	builds acid from
[Ref.: #65419]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65419]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65419]	D-mannose ChEBI	-	builds acid from
[Ref.: #65419]	D-ribose ChEBI	-	builds acid from
[Ref.: #65419]	D-serine ChEBI	+	oxidation
[Ref.: #65419]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65419]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65419]	D-trehalose ChEBI	-	builds acid from
[Ref.: #65419]	D-xylose ChEBI	-	builds acid from
[Ref.: #65419]	elastin ChEBI	-	assimilation
[Ref.: #65419]	erythritol ChEBI	-	builds acid from
[Ref.: #65419]	esculin ChEBI	-	builds acid from
[Ref.: #65419]	galactitol ChEBI	-	builds acid from
[Ref.: #65419]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65419]	glycerol 3-phosphate ChEBI	-	builds acid from
[Ref.: #65419]	glycogen ChEBI	-	builds acid from
[Ref.: #65419]	inulin ChEBI	-	builds acid from
[Ref.: #65419]	isocitrate ChEBI	+	assimilation
[Ref.: #65419]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65419]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65419]	L-fucose ChEBI	-	builds acid from
[Ref.: #65419]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65419]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65419]	L-xylose ChEBI	-	builds acid from
[Ref.: #65419]	lactose ChEBI	-	builds acid from
[Ref.: #65419]	maltose ChEBI	-	builds acid from
[Ref.: #65419]	melezitose ChEBI	-	builds acid from
[Ref.: #65419]	melibiose ChEBI	-	builds acid from
[Ref.: #65419]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65419]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65419]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65419]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65419]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65419]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65419]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65419]	raffinose ChEBI	-	builds acid from
[Ref.: #65419]	ribitol ChEBI	-	builds acid from
[Ref.: #65419]	salicin ChEBI	-	builds acid from
[Ref.: #65419]	starch ChEBI	-	assimilation
[Ref.: #65419]	sucrose ChEBI	-	builds acid from
[Ref.: #65419]	turanose ChEBI	-	builds acid from
[Ref.: #65419]	tween 20 ChEBI	-	assimilation
[Ref.: #65419]	tween 40 ChEBI	-	assimilation
[Ref.: #65419]	tween 60 ChEBI	-	assimilation
[Ref.: #65419]	tween 80 ChEBI	-	assimilation
[Ref.: #65419]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65419]		acetoin ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #65419]	acid phosphatase	+	3.1.3.2
[Ref.: #65419]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65419]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65419]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65419]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65419]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65419]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65419]	beta-galactosidase	-	3.2.1.23
[Ref.: #65419]	beta-glucosidase	-	3.2.1.21
[Ref.: #65419]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65419]	catalase	+	1.11.1.6
[Ref.: #65419]	cystine arylamidase	-	3.4.11.3
[Ref.: #65419]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65419]	esterase Lipase (C 8)	-
[Ref.: #65419]	gelatinase	+
[Ref.: #65419]	leucine arylamidase	+	3.4.11.1
[Ref.: #65419]	lipase (C 14)	-
[Ref.: #65419]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65419]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65419]	trypsin	-	3.4.21.4
[Ref.: #65419]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65419]

Fatty acid	Percentage
C15:0 anteiso	21.9
C15:0 iso 2OH and/or C16:1ω7c	12.7
C15:0 iso	8.8
C17:1 anteiso ω9c	6.7
C17:1 iso ω9c	6.1
C15:1 iso G	5.2
C16:0 iso	4.2
C17:0 iso 3OH	4.1
C15:0 iso 3OH	3.9
C17:0 2OH	3.4
C15:0	3
C16:1 iso H	2.9
C15:1 anteiso A	2.5
C15:0 2OH	1.8
C16:0 iso 3OH	1.7
C18:1ω5c	1.5
C15:1ω6c	1.2
C16:0	1.2
C14:0 iso	1.1
C16:0 3OH	0.9
C17:1ω6c	0.8
C17:0 anteiso	0.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67771]	Sample type/isolated from	From soil in a seal habitat on King George Island
[Ref.: #67771]	Geographic location (country and/or sea, region)	South Shetland Islands, Antarctica(62Â°11â€²10â€³S
[Ref.: #67771]	Country	Antarctica
[Ref.: #67771]	Country ISO 3 Code	ATA
[Ref.: #67771]	Continent	Australia and Oceania
[Ref.: #67771]	Geographic location	-62.1861°/-58.9936°

[Ref.: #65419]	Sample type/isolated from	soil in a seal habitat on King George Island
[Ref.: #65419]	Geographic location (country and/or sea, region)	South Shetland Islands
[Ref.: #65419]	Country	Antarctica
[Ref.: #65419]	Country ISO 3 Code	ATA
[Ref.: #65419]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Aquatic mammal
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX911210 (>99% sequence identity) for Flavobacterium phocarum subclade from Microbeatlas

Aquatic Samples	1310
Soil Samples	674
Animal Samples	2314
Plant Samples	179
Total Samples	4477

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65419]	Flavobacterium phocarum strain SE14 16S ribosomal RNA gene, partial sequence	KX911210		GenBank ENA

[Ref.: #65419]	GC-content	42.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67771]	GC-content	42.3 mol%

Availability in culture collections External links

[Ref.: #65419]

Culture collection no.

CCTCC AB 2017225, KCTC 52612

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium phocarum sp. nov., isolated from soils of a seal habitat in Antarctica.	Zhou MY, Zhang YJ, Zhang XY, Yang XD, He HL, Ning D, Du Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.002535	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65419

Ming-Yang Zhou, Yan-Jiao Zhang, Xi-Ying Zhang, Xiao-Deng Yang, Hai-Lun He, Daliang Ning, Zongjun Du: Flavobacterium phocarum sp. nov., isolated from soils of a seal habitat in Antarctica. IJSEM 68: 536 - 541 2018 ( DOI 10.1099/ijsem.0.002535 , PubMed 29251588 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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