Name: Phyllobacterium salinisoli LLAN61
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158644

Type strain:

Species: Phyllobacterium salinisoli

Strain Designation: LLAN61

Culture col. no.: LMG 30173, CECT 9417

NCBI tax ID(s): 1899321 (species)

Section

Phyllobacterium salinisoli LLAN61 is an aerobe, Gram-negative, motile bacterium that was isolated from root nodule of Lotus lancerottensis growing in a saline soil sample.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Phyllobacteriaceae*
	Genus	*Phyllobacterium*
	Species	**Phyllobacterium salinisoli**
	Full Scientific Name (LPSN)	Phyllobacterium salinisoli León-Barrios et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65593]	Gram stain	negative

[Ref.: #65593]	Cell shape	rod-shaped

[Ref.: #65593]	Motility	yes

[Ref.: #65593]	Flagellum arrangement	subpolar

[Ref.: #65593]	Colony size	<2 mm
[Ref.: #65593]	Colony color	pearl white
[Ref.: #65593]	Incubation period	3 days
[Ref.: #65593]	Cultivation medium used	yeast mannitol agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65593]

Culture medium

yeast mannitol agar

[Ref.: #65593]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #65593]			growth	6-37 °C
[Ref.: #65593]			optimum	28 °C

[Ref.: #65593]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #65593]			optimum	7
[Ref.: #65593]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65593]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65593]		NaCl		growth	0.5-3.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65593]	2-dehydro-D-gluconate ChEBI	+	assimilation
[Ref.: #65593]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65593]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65593]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65593]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #65593]	acetate ChEBI	+	assimilation
[Ref.: #65593]	adipate ChEBI	-	assimilation
[Ref.: #65593]	citrate ChEBI	-	assimilation
[Ref.: #65593]	D-glucose ChEBI	+	assimilation
[Ref.: #65593]	D-mannitol ChEBI	+	assimilation
[Ref.: #65593]	D-mannitol ChEBI	+	carbon source
[Ref.: #65593]	D-mannose ChEBI	+	carbon source
[Ref.: #65593]	D-ribose ChEBI	+	assimilation
[Ref.: #65593]	D-sorbitol ChEBI	+	assimilation
[Ref.: #65593]	decanoate ChEBI	-	assimilation
[Ref.: #65593]	esculin ChEBI	-	assimilation
[Ref.: #65593]	gluconate ChEBI	-	assimilation
[Ref.: #65593]	glycogen ChEBI	-	assimilation
[Ref.: #65593]	itaconate ChEBI	-	assimilation
[Ref.: #65593]	L-alanine ChEBI	+	assimilation
[Ref.: #65593]	L-arabinose ChEBI	+	assimilation
[Ref.: #65593]	L-arabinose ChEBI	+	carbon source
[Ref.: #65593]	L-fucose ChEBI	+	assimilation
[Ref.: #65593]	L-histidine ChEBI	+	assimilation
[Ref.: #65593]	L-proline ChEBI	+	assimilation
[Ref.: #65593]	L-rhamnose ChEBI	+	assimilation
[Ref.: #65593]	L-serine ChEBI	-	assimilation
[Ref.: #65593]	lactate ChEBI	+	assimilation
[Ref.: #65593]	malate ChEBI	+	carbon source
[Ref.: #65593]	malonate ChEBI	-	assimilation
[Ref.: #65593]	maltose ChEBI	-	assimilation
[Ref.: #65593]	melibiose ChEBI	+	assimilation
[Ref.: #65593]	myo-inositol ChEBI	+	assimilation
[Ref.: #65593]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65593]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #65593]	nitrate ChEBI	-	reduction
[Ref.: #65593]	phenylacetate ChEBI	-	assimilation
[Ref.: #65593]	propionate ChEBI	-	assimilation
[Ref.: #65593]	salicin ChEBI	-	assimilation
[Ref.: #65593]	suberic acid ChEBI	-	assimilation
[Ref.: #65593]	sucrose ChEBI	-	assimilation
[Ref.: #65593]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65593]	ampicillin ChEBI				yes	2	µg (disc)
[Ref.: #65593]	cefuroxime ChEBI				yes	30	µg (disc)
[Ref.: #65593]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #65593]	cloxacillin ChEBI				yes	1	µg (disc)
[Ref.: #65593]	erythromycin ChEBI				yes	2	µg (disc)
[Ref.: #65593]	gentamicin ChEBI				yes	30	µg (disc)
[Ref.: #65593]	neomycin ChEBI				yes	5	µg (disc)
[Ref.: #65593]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #65593]	polymyxin b ChEBI				yes	300	Unit (disc)

	Enzyme	Enzyme activity	EC number
[Ref.: #65593]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65593]	beta-galactosidase	+	3.2.1.23
[Ref.: #65593]	catalase	+	1.11.1.6
[Ref.: #65593]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65593]	gelatinase	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65593]	Sample type/isolated from	root nodule of Lotus lancerottensis growing in a saline soil sample
[Ref.: #65593]	Geographic location (country and/or sea, region)	Salinas del Janubio, Lanzarote (Canary Islands)
[Ref.: #65593]	Country	Spain
[Ref.: #65593]	Country ISO 3 Code	ESP
[Ref.: #65593]	Continent	Europe
[Ref.: #65593]	Enrichment culture	modified yeast mannitol agar
[Ref.: #65593]	Enrichment culture composition	(YMA; [10]; 10 g l-1 mannitol, 1 g l-1 yeast extract, 0.2 g l-1 K2HPO4 , 0.2 g l-1 MgSO4 x 7H2O, 0.5 g l-1 NaCl, 20 g l-1 agar)
[Ref.: #65593]	Enrichment culture duration	5 days
[Ref.: #65593]	Enrichment culture temperature	28 ̊C
[Ref.: #65593]	Isolation/enrichment procedure	The root nodules were surface sterilized for 1 min in 90 % (v/v) ethanol followed by 3 min in 2 % (v/v) NaClO, and then washed six times in sterile distilled water. The nodules were then crushed using a sterile glass rod and the homogenized nodule tissue was inoculated on modified yeast mannitol agar and the plates were incubated at 28°C for 5 days.
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule
#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT614649 (>99% sequence identity) for Phyllobacterium from Microbeatlas

Aquatic Samples	969
Soil Samples	1470
Animal Samples	3031
Plant Samples	984
Total Samples	6454

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65593]	16S rRNA gene sequence	LT614649		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Phyllobacterium salinisoli LLAN61	GCA_003335045	contig	GenBank	1899321 tax ID	*
[Ref.: #66792]	Phyllobacterium salinisoli LLAN61	2840750342	draft	JGI IMG/M	1899321 tax ID	*

[Ref.: #65593]

GC-content

58 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65593]

Culture collection no.

LMG 30173, CECT 9417

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phyllobacterium phragmitis sp. nov., an endophytic bacterium isolated from Phragmites australis rhizome in Kumtag Desert.	Liang LX, Sun QW, Hui N, Zhang XX, Li LB, Liu L	Antonie Van Leeuwenhoek	10.1007/s10482-018-1195-5	2018	*
Phylogeny	Phyllobacterium salinisoli sp. nov., isolated from a Lotus lancerottensis root nodule in saline soil from Lanzarote.	Leon-Barrios M, Ramirez-Bahena MH, Igual JM, Peix A, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002628	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65593

Milagros León-Barrios, Martha Helena Ramírez-Bahena,José M. Igual, Álvaro Peix, Encarna Velázquez: Phyllobacterium salinisoli sp. nov., isolated from a Lotus lancerottensis root nodule in saline soil from Lanzarote. IJSEM 68: 1085 - 1089 2018 ( DOI 10.1099/ijsem.0.002628 , PubMed 29458536 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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