Micromonospora globbae (KCTC 39787, NBRC 112325, TISTR 2405)

Name: Micromonospora globbae (KCTC 39787, NBRC 112325, TISTR 2405)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158643

Type strain

Strain Designation WPS1-2

Culture col. no. KCTC 39787 NBRC 112325 TISTR 2405 WPS1-2 JCM 32902 1 more

NCBI tax ID(s) 1894969

Special Collections JCM KCTC Literature strains

History <- Somboon Tanasupawat S. Tanasupawat; Chulalongkorn Univ., Thailand; WPS1-2.

Links

Micromonospora globbae WPS1-2 is an aerobe, spore-forming, mesophilic prokaryote that was isolated from roots of Globba winitii C. H. Wright.

spore-forming Gram-positive aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 283072

DSM 117320,KCTC 39787,NBRC 112325

@ref 20215

Last LPSN update 2026-02-09 11:38:23

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora globbae

Full scientific name Micromonospora globbae Kuncharoen et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Confidence
65368	positive
125439	positive	94.8

Colony morphology

@ref	Colony color	Incubation period	Medium used
65368	vivid orange-yellow	14 days	ISP2

Multicellular morphology

@ref	Forms multicellular complex	Medium name	Further description
65368		ISP2	mesophilic actinomycete which forms single spores (1.1 – 1.2 µm) directly on substrate mycelium.

Multimedia

@ref:	125233
multimedia content:	DSM_117320.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_117320.jpg
caption:	Medium 554 28°C
intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
65368	ISP2
125233	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
125233	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
125233	ISP2 MEDIUM (DSMZ Medium 987)	Medium recipe at MediaDive	Name: ISP2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
65368	positive	optimum	30-37	mesophilic
67770	positive	growth	28	mesophilic
67771	positive	growth	28	mesophilic
125233	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
65368	positive	growth	5-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65368	aerobe
67771	aerobe
125439	obligate aerobe	99.5

Spore formation

@ref	Spore description	Spore formation
65368	single spores (1.1-1.2 µm)

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65368	NaCl	positive	maximum	3 %(w/v)

Observation

@ref	Observation
65368	Whole-cell hydrolysates contain ribose, xylose, arabinose and glucose
67770	quinones: MK-10(H₈), MK-10(H₁₀)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65368	17057 ChEBI	cellobiose	-	carbon source
65368	15824 ChEBI	D-fructose	-	carbon source
65368	16899 ChEBI	D-mannitol	+	carbon source
65368	65327 ChEBI	D-xylose	-	carbon source
65368	28260 ChEBI	galactose	-	carbon source
65368	5291 ChEBI	gelatin	-	hydrolysis
65368	17234 ChEBI	glucose	+	carbon source
65368	17754 ChEBI	glycerol	+	carbon source
65368	30849 ChEBI	L-arabinose	-	carbon source
65368	37684 ChEBI	mannose	+	carbon source
65368	17268 ChEBI	myo-inositol	+	carbon source
65368	17632 ChEBI	nitrate	-	reduction
65368	16634 ChEBI	raffinose	+	carbon source
65368	26546 ChEBI	rhamnose	-	carbon source
65368	33942 ChEBI	ribose	-	carbon source
65368	17814 ChEBI	salicin	+	carbon source
65368		skimmed milk	+	assimilation
65368	27922 ChEBI	sorbose	-	carbon source
65368	28017 ChEBI	starch	+	hydrolysis
65368	17992 ChEBI	sucrose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
65368	acid phosphatase	+	3.1.3.2
65368	alkaline phosphatase	+	3.1.3.1
65368	alpha-chymotrypsin	+	3.4.21.1
65368	alpha-fucosidase	-	3.2.1.51
65368	alpha-galactosidase	+	3.2.1.22
65368	alpha-glucosidase	+	3.2.1.20
65368	alpha-mannosidase	-	3.2.1.24
65368	beta-galactosidase	+	3.2.1.23
65368	beta-glucosidase	+	3.2.1.21
65368	beta-glucuronidase	-	3.2.1.31
65368	cystine arylamidase	-	3.4.11.3
65368	esterase (C 4)	+
65368	esterase Lipase (C 8)	+
65368	leucine arylamidase	+	3.4.11.1
65368	lipase (C 14)	-
65368	N-acetyl-beta-glucosaminidase	+	3.2.1.52
65368	naphthol-AS-BI-phosphohydrolase	+
65368	trypsin	+	3.4.21.4
65368	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

65368

Fatty acid	Percentage
C15:0 iso	27.6
C16:0 iso	14.1
C15:0 anteiso	10.3
C17:0 anteiso	9.9
C17:0 iso	6.1
C17:1ω8c	5.6
C17:0	5.5
C15:1 iso G	3.4
C16:1 iso G	2.3
C16:0	1.8
10-Methyl C17:0	1.5
10-methyl C16:0 or iso-C17:1ω9c	1.4
C14:0 iso	1.3
C16:1ω7c or C16:1ω6c	1.3
C18:0	1.1
C17:1 anteiso ω9c	1.1
C18:1ω9c	1
C15:1ω6c	0.6
C16:1 2OH	0.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure	Host species
65368	roots of Globba winitii C. H. Wright	botanical garden of the Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok	Thailand	THA	Asia	starch casein gellan gum medium	supplemented with 25 µg nalidixic acid ml-1 and 50 µg cycloheximide ml-1	21 days	30	The root samples were cut to 2 cm in length, then soaked in 75 % (v/v) ethanol for 3 min and subsequently immersed in 3 % (v/v) NaOCl for 10 min. Surface-sterilized roots were washed three times with sterile distilled water. The roots were soaked in 10 % (w/v) NaHCO3 for 10 min to disrupt root tissues and delay the growth of fungi. The roots were crushed with 4 % (w/v) sucrose solution in a sterile motar. The crushed roots suspension was incubated at 60 C in a water bath for 10 min. The suspen
67770	Roots of Globba winitii C. H. Wright from the botanical garden of the Fac. of Pharm. Sci.	Chulalongkorn Univ., Bangkok	Thailand	THA	Asia						Globba winitii
67771	from a root of `Globba winitii`		Thailand	THA	Asia
125233	roots of Globba winitii	botanical garden of the Fac- ulty of Pharmaceutical Sciences, Chulalongkorn University,Bangkok	Thailand	THA	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM361078v1 assembly for Micromonospora globbae WPS1-2	contig	GCA_003610785	1894969.3	2867302475	1894969	55.79

16S sequences

@ref	Description	Accession	Database
65368	Micromonospora globbae WPS1-2 gene for 16S ribosomal RNA, partial sequence	LC177396

GC content

@ref	GC-content (mol%)	Method
65368	73.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Micromonospora globbae WPS1-2
NCBI: GCA_003610785

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	64.30	no
125439	motility	BacteriaNetⓘ	no	72.90	no
125439	gram_stain	BacteriaNetⓘ	positive	94.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Stage dependent microbial dynamics in hepatocellular carcinoma and adjacent normal liver tissues.	Jo HE, Khom S, Lee S, Cho SH, Park SY, You GR, Kim H, Kim NI, Jeong JH, Yoon JH, Yun M.	Sci Rep	10.1038/s41598-024-77260-6	2024
Phylogeny	Micromonospora globbae sp. nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.	Kuncharoen N, Pittayakhajonwut P, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002625	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65368	Nattakorn Kuncharoen, Pattama Pittayakhajonwut, Somboon Tanasupawat: Micromonospora globbae sp. nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright. IJSEM 68: 1073 - 1077 2018 ( DOI 10.1099/ijsem.0.002625 , PubMed 29458491 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#125233	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 117320
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora globbae (KCTC 39787, NBRC 112325, TISTR 2405)

Name and taxonomic classification