Name: Micromonospora globbae WPS1-2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158643

Type strain:

Species: Micromonospora globbae

Strain Designation: WPS1-2

Culture col. no.: KCTC 39787, NBRC 112325, TISTR 2405, JCM 32902

Strain history: S. Tanasupawat; Chulalongkorn Univ., Thailand; WPS1-2.

NCBI tax ID(s): 1894969 (species)

Special Collections

Section

Micromonospora globbae WPS1-2 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from roots of Globba winitii C. H. Wright.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micromonosporales*
	Family	*Micromonosporaceae*
	Genus	*Micromonospora*
	Species	**Micromonospora globbae**
	Full Scientific Name (LPSN)	Micromonospora globbae Kuncharoen et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65368]	Gram stain	positive

[Ref.: #65368]	Colony color	vivid orange-yellow
[Ref.: #65368]	Incubation period	14 days
[Ref.: #65368]	Cultivation medium used	ISP2

[Ref.: #65368]	Multicellular complex forming ability	yes
[Ref.: #65368]	Medium Name (multicellularity)	ISP2
[Ref.: #65368]	Multicellular complex further description	mesophilic actinomycete which forms single spores (1.1 – 1.2 µm) directly on substrate mycelium.

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65368]

Culture medium

ISP2

[Ref.: #65368]

Culture medium growth

		Temperature
[Ref.: #65368]	optimum	30-37 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #65368]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65368]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65368]	Spore description	single spores (1.1-1.2 µm)
[Ref.: #65368]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65368]		NaCl		maximum	3	%(w/v)

[Ref.: #65368]	Observation	Whole-cell hydrolysates contain ribose, xylose, arabinose and glucose
[Ref.: #67770]	Observation	quinones: MK-10(H₈), MK-10(H₁₀)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65368]	cellobiose ChEBI	-	carbon source
[Ref.: #65368]	D-fructose ChEBI	-	carbon source
[Ref.: #65368]	D-mannitol ChEBI	+	carbon source
[Ref.: #65368]	D-xylose ChEBI	-	carbon source
[Ref.: #65368]	galactose ChEBI	-	carbon source
[Ref.: #65368]	gelatin ChEBI	-	hydrolysis
[Ref.: #65368]	glucose ChEBI	+	carbon source
[Ref.: #65368]	glycerol ChEBI	+	carbon source
[Ref.: #65368]	L-arabinose ChEBI	-	carbon source
[Ref.: #65368]	mannose ChEBI	+	carbon source
[Ref.: #65368]	myo-inositol ChEBI	+	carbon source
[Ref.: #65368]	nitrate ChEBI	-	reduction
[Ref.: #65368]	raffinose ChEBI	+	carbon source
[Ref.: #65368]	rhamnose ChEBI	-	carbon source
[Ref.: #65368]	ribose ChEBI	-	carbon source
[Ref.: #65368]	salicin ChEBI	+	carbon source
[Ref.: #65368]	skimmed milk	+	assimilation
[Ref.: #65368]	sorbose ChEBI	-	carbon source
[Ref.: #65368]	starch ChEBI	+	hydrolysis
[Ref.: #65368]	sucrose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65368]	acid phosphatase	+	3.1.3.2
[Ref.: #65368]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65368]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #65368]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65368]	alpha-galactosidase	+	3.2.1.22
[Ref.: #65368]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65368]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65368]	beta-galactosidase	+	3.2.1.23
[Ref.: #65368]	beta-glucosidase	+	3.2.1.21
[Ref.: #65368]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65368]	cystine arylamidase	-	3.4.11.3
[Ref.: #65368]	esterase (C 4)	+
[Ref.: #65368]	esterase Lipase (C 8)	+
[Ref.: #65368]	leucine arylamidase	+	3.4.11.1
[Ref.: #65368]	lipase (C 14)	-
[Ref.: #65368]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #65368]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65368]	trypsin	+	3.4.21.4
[Ref.: #65368]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65368]

Fatty acid	Percentage
C15:0 iso	27.6
C16:0 iso	14.1
C15:0 anteiso	10.3
C17:0 anteiso	9.9
C17:0 iso	6.1
C17:1ω8c	5.6
C17:0	5.5
C15:1 iso G	3.4
C16:1 iso G	2.3
C16:0	1.8
10-Methyl C17:0	1.5
10-methyl C16:0 or iso-C17:1ω9c	1.4
C14:0 iso	1.3
C16:1ω7c or C16:1ω6c	1.3
C18:0	1.1
C17:1 anteiso ω9c	1.1
C18:1ω9c	1
C15:1ω6c	0.6
C16:1 2OH	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65368]	Sample type/isolated from	roots of Globba winitii C. H. Wright
[Ref.: #65368]	Geographic location (country and/or sea, region)	botanical garden of the Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok
[Ref.: #65368]	Country	Thailand
[Ref.: #65368]	Country ISO 3 Code	THA
[Ref.: #65368]	Continent	Asia
[Ref.: #65368]	Enrichment culture	starch casein gellan gum medium
[Ref.: #65368]	Enrichment culture composition	supplemented with 25 µg nalidixic acid ml-1 and 50 µg cycloheximide ml-1
[Ref.: #65368]	Enrichment culture duration	21 days
[Ref.: #65368]	Enrichment culture temperature	30 ̊C
[Ref.: #65368]	Isolation/enrichment procedure	The root samples were cut to 2 cm in length, then soaked in 75 % (v/v) ethanol for 3 min and subsequently immersed in 3 % (v/v) NaOCl for 10 min. Surface-sterilized roots were washed three times with sterile distilled water. The roots were soaked in 10 % (w/v) NaHCO3 for 10 min to disrupt root tissues and delay the growth of fungi. The roots were crushed with 4 % (w/v) sucrose solution in a sterile motar. The crushed roots suspension was incubated at 60 C in a water bath for 10 min. The suspen

[Ref.: #67770]	Sample type/isolated from	Roots of Globba winitii C. H. Wright from the botanical garden of the Fac. of Pharm. Sci.
[Ref.: #67770]	Host species	Globba winitii
[Ref.: #67770]	Geographic location (country and/or sea, region)	Chulalongkorn Univ., Bangkok
[Ref.: #67770]	Country	Thailand
[Ref.: #67770]	Country ISO 3 Code	THA
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	from a root of `Globba winitii`
[Ref.: #67771]	Country	Thailand
[Ref.: #67771]	Country ISO 3 Code	THA
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65368]	Micromonospora globbae WPS1-2 gene for 16S ribosomal RNA, partial sequence	LC177396		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Micromonospora globbae WPS1-2	GCA_003610785	contig	GenBank ENA	1894969 tax ID
[Ref.: #66792]	Micromonospora globbae WPS1-2	2867302475	draft	JGI IMG/M	1894969 tax ID	*

[Ref.: #65368]

GC-content

73.7 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65368]

Culture collection no.

KCTC 39787, NBRC 112325, TISTR 2405, JCM 32902

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Micromonospora globbae sp. nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright.	Kuncharoen N, Pittayakhajonwut P, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002625	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65368

Nattakorn Kuncharoen, Pattama Pittayakhajonwut, Somboon Tanasupawat: Micromonospora globbae sp. nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright. IJSEM 68: 1073 - 1077 2018 ( DOI 10.1099/ijsem.0.002625 , PubMed 29458491 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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