Name: Paenibacillus limicola CJ6
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158601

Type strain:

Species: Paenibacillus limicola

Strain Designation: CJ6

Culture col. no.: KACC 19303, JCM 32079

Strain history: C.-J. Cha; Chung-Ang Univ., South Korea; CJ6.

NCBI tax ID(s): 2060098 (species)

Section

Paenibacillus limicola CJ6 is an aerobe, spore-forming, Gram-variable bacterium that forms circular colonies and was isolated from tidal flat.

colony-forming
Gram-variable
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus limicola**
	Full Scientific Name (LPSN)	Paenibacillus limicola Nahar and Cha 2018

Information on morphological and physiological properties Morphology

[Ref.: #65317]	Gram stain	variable

[Ref.: #65317]	Cell length	1.8-2.8 µm

[Ref.: #65317]	Cell width	0.5-0.8 µm

[Ref.: #65317]	Cell shape	rod-shaped

[Ref.: #65317]	Motility	yes

[Ref.: #65317]	Colony color	white
[Ref.: #65317]	Colony shape	circular
[Ref.: #65317]	Incubation period	5 days
[Ref.: #65317]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

tryptic soy agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

		Temperature
[Ref.: #65317]	growth	15-37 °C
[Ref.: #65317]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #65317]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65317]			optimum	7-8
[Ref.: #65317]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #65317]	Type of spore	endospore
[Ref.: #65317]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65317]		NaCl		growth	0-1	%

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Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65317]	4-nitrophenyl beta-D-galactopyranoside hydrolysate	+	assimilation
[Ref.: #65317]	amygdalin ChEBI	+	builds acid from
[Ref.: #65317]	arabinose ChEBI	-	assimilation
[Ref.: #65317]	arbutin ChEBI	+	builds acid from
[Ref.: #65317]	casein	-	hydrolysis
[Ref.: #65317]	cellulose ChEBI	-	hydrolysis
[Ref.: #65317]	D-fructose ChEBI	-	builds acid from
[Ref.: #65317]	D-galactose ChEBI	-	builds acid from
[Ref.: #65317]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65317]	D-xylose ChEBI	-	builds acid from
[Ref.: #65317]	dna ChEBI	-	hydrolysis
[Ref.: #65317]	esculin ferric citrate	+	builds acid from
[Ref.: #65317]	gelatin ChEBI	+	hydrolysis
[Ref.: #65317]	gentiobiose ChEBI	+	builds acid from
[Ref.: #65317]	glucose ChEBI	+	assimilation
[Ref.: #65317]	glycerol ChEBI	+	builds acid from
[Ref.: #65317]	glycogen ChEBI	+	builds acid from
[Ref.: #65317]	malate ChEBI	-	assimilation
[Ref.: #65317]	maltose ChEBI	+	builds acid from
[Ref.: #65317]	maltose ChEBI	+	assimilation
[Ref.: #65317]	mannitol ChEBI	-	assimilation
[Ref.: #65317]	mannose ChEBI	-	assimilation
[Ref.: #65317]	melezitose ChEBI	-	builds acid from
[Ref.: #65317]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #65317]	methyl alpha-D-mannoside ChEBI	+	builds acid from
[Ref.: #65317]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65317]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65317]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65317]	nitrate ChEBI	-	reduction
[Ref.: #65317]	raffinose ChEBI	+	builds acid from
[Ref.: #65317]	salicin ChEBI	+	builds acid from
[Ref.: #65317]	starch ChEBI	+	builds acid from
[Ref.: #65317]	starch ChEBI	+	hydrolysis
[Ref.: #65317]	turanose ChEBI	+	builds acid from
[Ref.: #65317]	urea ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65317]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65317]	acid phosphatase	+	3.1.3.2
[Ref.: #65317]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65317]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65317]	alpha-fucosidase	+	3.2.1.51
[Ref.: #65317]	alpha-galactosidase	+	3.2.1.22
[Ref.: #65317]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65317]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65317]	beta-galactosidase	+	3.2.1.23
[Ref.: #65317]	beta-glucosidase	-	3.2.1.21
[Ref.: #65317]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65317]	catalase	+	1.11.1.6
[Ref.: #65317]	cystine arylamidase	-	3.4.11.3
[Ref.: #65317]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65317]	esterase (C 4)	+
[Ref.: #65317]	esterase Lipase (C 8)	+
[Ref.: #65317]	leucine arylamidase	+	3.4.11.1
[Ref.: #65317]	lipase (C 14)	-
[Ref.: #65317]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65317]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65317]	trypsin	+	3.4.21.4
[Ref.: #65317]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65317]

Fatty acid	Percentage
C15:0 anteiso	39.7
C16:0	6.8
C16:0 iso	6.4
C15:0 iso	5.4
C12:0	5.2
C10:0	3.6
C14:0 iso	3.6
C17:0 anteiso	2.9
C13:0	2.4
C17:0 iso	2.2
C11:0 iso 3OH	2
C14:0	2
C11:0 2OH	1.6
C17:1 iso ω5c	1.6
iso-C15:1ω9c	1.4
C19:1ω6c and/or C19:1ω7c	1.3
C11:0 3OH	1.2
C10:0 iso	1.2
C12:0 iso	1.1
cyclo-C19:0ω8c	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65317]	Sample type/isolated from	tidal flat
[Ref.: #65317]	Geographic location (country and/or sea, region)	Ganghwa Island
[Ref.: #65317]	Country	Republic of Korea
[Ref.: #65317]	Country ISO 3 Code	KOR
[Ref.: #65317]	Continent	Asia
[Ref.: #65317]	Geographic location	37.6162°/126.3770°
[Ref.: #65317]	Enrichment culture	R2A
[Ref.: #65317]	Enrichment culture duration	3 days
[Ref.: #65317]	Enrichment culture temperature	30 ̊C
[Ref.: #65317]	Isolation/enrichment procedure	standard dilution plating technique

[Ref.: #67770]	Sample type/isolated from	Tidal flat on Ganghwa Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Tidal flat

What are isolation sources categories?

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Global distribution of 16S sequence KY056224 (>99% sequence identity) for Paenibacillus limicola subclade from Microbeatlas

Aquatic Samples
Soil Samples	8
Animal Samples
Plant Samples
Total Samples	8

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65317]	16S rRNA gene sequence	KY056224		GenBank

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GC-content

49.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65317]

Culture collection no.

KACC 19303, JCM 32079

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus limicola sp. nov., isolated from tidal flat sediment.	Nahar S, Cha CJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002522	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65317

Shamsun Nahar, Chang-Jun Cha: Paenibacillus limicola sp. nov., isolated from tidal flat sediment. IJSEM 68: 423 - 426 2018 ( DOI 10.1099/ijsem.0.002522 , PubMed 29235981 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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