Asprobacter aquaticus (KCTC 42356, JCM 30469, DRW22-8)

Name: Asprobacter aquaticus (KCTC 42356, JCM 30469, DRW22-8)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158566

Type strain

Strain Designation DRW22-8

Culture col. no. KCTC 42356 JCM 30469 DRW22-8

NCBI tax ID(s) 1348470

Special Collections JCM KCTC Literature strains

History L. Jin; KRIBB, South Korea; DRW22-8. <- Long Jin, KRIBB

Links

Asprobacter aquaticus DRW22-8 is an aerobe, Gram-negative, ovoid-shaped bacterium that forms circular colonies and was isolated from fresh water sample at a depth of 22 m.

Gram-negative ovoid-shaped colony-forming aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 25797

KCTC 42356,JCM 30469

@ref 20215

Last LPSN update 2026-02-09 11:38:22

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Caulobacterales

Family Hyphomonadaceae

Genus Asprobacter

Species Asprobacter aquaticus

Full scientific name Asprobacter aquaticus Jin et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
65266	negative	ovoid-shaped

Colony morphology

@ref	Colony color	Colony shape	Medium used
65266	creamy-white	circular	R2A

Culture and growth conditions

Culture medium

@ref	Name	Growth
65266	Reasoner's 2A agar (R2A)
65266	tryptic soy agar
65266	LB (Luria-Bertani) MEDIUM

Culture temp

@ref	Growth	Type	Temperature (°C)
65266	positive	growth	15-37
65266	positive	optimum	30
65266	negative	growth	8
65266	negative	growth	42
67770	positive	growth	30
67771	positive	growth	30

Culture pH

@ref	Ability	Type	PH
65266	positive	growth	7
65266		growth	6
65266		growth	8

Physiology and metabolism

Oxygen tolerance

67771

Oxygen toleranceaerobe

Spore formation

65266

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65266	NaCl	positive	growth	1 %(w/v)
65266	NaCl		growth	2 %(w/v)

Observation

67770

Observationquinones: Q-10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65266	16808 ChEBI	2-dehydro-D-gluconate	-	assimilation
65266	16193 ChEBI	3-hydroxybenzoate	-	assimilation
65266	37054 ChEBI	3-hydroxybutyrate	-	assimilation
65266	17879 ChEBI	4-hydroxybenzoate	-	assimilation
65266	58143 ChEBI	5-dehydro-D-gluconate	-	assimilation
65266	30089 ChEBI	acetate	-	assimilation
65266	17128 ChEBI	adipate	-	assimilation
65266	16947 ChEBI	citrate	-	assimilation
65266	17634 ChEBI	D-glucose	-	assimilation
65266	16899 ChEBI	D-mannitol	+	assimilation
65266	16024 ChEBI	D-mannose	-	assimilation
65266	16988 ChEBI	D-ribose	-	assimilation
65266	17924 ChEBI	D-sorbitol	-	assimilation
65266	27689 ChEBI	decanoate	-	assimilation
65266	4853 ChEBI	esculin	+	hydrolysis
65266	5291 ChEBI	gelatin	-	hydrolysis
65266	24265 ChEBI	gluconate	-	assimilation
65266	17234 ChEBI	glucose	-	fermentation
65266	28087 ChEBI	glycogen	-	assimilation
65266	17240 ChEBI	itaconate	-	assimilation
65266	16977 ChEBI	L-alanine	-	assimilation
65266	30849 ChEBI	L-arabinose	-	assimilation
65266	18287 ChEBI	L-fucose	-	assimilation
65266	15971 ChEBI	L-histidine	-	assimilation
65266	17203 ChEBI	L-proline	+	assimilation
65266	62345 ChEBI	L-rhamnose	+	assimilation
65266	17115 ChEBI	L-serine	+	assimilation
65266	24996 ChEBI	lactate	-	assimilation
65266	25115 ChEBI	malate	-	assimilation
65266	15792 ChEBI	malonate	-	assimilation
65266	17306 ChEBI	maltose	-	assimilation
65266	28053 ChEBI	melibiose	-	assimilation
65266	17268 ChEBI	myo-inositol	-	assimilation
65266	506227 ChEBI	N-acetylglucosamine	-	assimilation
65266	17632 ChEBI	nitrate	-	reduction
65266	18401 ChEBI	phenylacetate	-	assimilation
65266	17272 ChEBI	propionate	-	assimilation
65266	17814 ChEBI	salicin	-	assimilation
65266	9300 ChEBI	suberic acid	-	assimilation
65266	17992 ChEBI	sucrose	-	assimilation
65266	31011 ChEBI	valerate	-	assimilation

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
65266	28971	ampicillin	50 µg/mL
65266	28971	ampicillin		10 µg/mL
65266	28971	ampicillin		30 µg/mL
65266	24753	hygromycin		10 µg/mL
65266	24753	hygromycin		30 µg/mL
65266	24753	hygromycin		50 µg/mL
65266	471744	imipenem		10 µg/mL
65266	471744	imipenem		30 µg/mL
65266	471744	imipenem		50 µg/mL
65266	18208	penicillin g	30 µg/mL
65266	18208	penicillin g	50 µg/mL
65266	18208	penicillin g		10 µg/mL
65266	17076	streptomycin	10 µg/mL
65266	17076	streptomycin	30 µg/mL
65266	17076	streptomycin	50 µg/mL
65266	27902	tetracycline	30 µg/mL
65266	27902	tetracycline	50 µg/mL
65266	27902	tetracycline		10 µg/mL

Metabolite production

@ref	Chebi-ID	Metabolite	Production
65266	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
65266	acid phosphatase	+	3.1.3.2
65266	alkaline phosphatase	+	3.1.3.1
65266	alpha-chymotrypsin	-	3.4.21.1
65266	alpha-fucosidase	-	3.2.1.51
65266	alpha-galactosidase	-	3.2.1.22
65266	alpha-glucosidase	-	3.2.1.20
65266	alpha-mannosidase	-	3.2.1.24
65266	arginine dihydrolase	-	3.5.3.6
65266	beta-glucosidase	-	3.2.1.21
65266	beta-glucuronidase	-	3.2.1.31
65266	catalase	-	1.11.1.6
65266	cystine arylamidase	-	3.4.11.3
65266	cytochrome oxidase	+	1.9.3.1
65266	esterase (C 4)	+
65266	esterase Lipase (C 8)	+
65266	leucine arylamidase	+	3.4.11.1
65266	lipase (C 14)	-
65266	N-acetyl-beta-glucosaminidase	-	3.2.1.52
65266	naphthol-AS-BI-phosphohydrolase	+
65266	trypsin	-	3.4.21.4
65266	urease	-	3.5.1.5
65266	valine arylamidase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
65266	fresh water sample at a depth of 22 m	Daechung Reservoir	Republic of Korea	KOR	Asia	modified 1/10 R2A agar	(L-1: 0.05 g peptone, 0.05 g yeast extract, 0.05 g casamino acid, 0.05 g dextrose, 0.05 g soluble starch, 0.03 g K2HPO4 , 0.005 g MgSO4 , 0.03 g sodium pyruvate and 15 g agar)	4 weeks	25	A 100 µl subsample (10-5 or 10-6) of the suspended material was spread onto modified 1/10 R2A agar and incubated at room temperature (25°C) for 4 weeks.
67770	Fresh water taken at a depth of 22 m at Daechung Reservoir		Republic of Korea	KOR	Asia
67771	From microcystis culture		Republic of Korea	KOR	Asia

Taxonmaps

69479

Global distribution of 16S sequence KF056993 (>99% sequence identity) for Asprobacter aquaticus subclade from Microbeatlas

Aquatic Samples	8470
Soil Samples	6086
Animal Samples	1245
Plant Samples	904
Total Samples	16705

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
65266	Asprobacter aquaticus strain DRW22-8 16S ribosomal RNA gene, partial sequence	KF056993

GC content

@ref	GC-content (mol%)	Method
65266	64.4	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water.	Jin L, Ko SR, Lee CS, Ahn CY, Oh HM, Lee HG	Int J Syst Evol Microbiol	10.1099/ijsem.0.002311	2017
Phylogeny	Pseudaquidulcibacter saccharophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.	Liu Y, Li S, Lin J, Liu S, Wei H, Dai J, Qiu D	Int J Syst Evol Microbiol	10.1099/ijsem.0.005264	2022
Phylogeny	Terricaulis silvestris gen. nov., sp. nov., a novel prosthecate, budding member of the family Caulobacteraceae isolated from forest soil.	Vieira S, Pascual J, Boedeker C, Geppert A, Riedel T, Rohde M, Overmann J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004367	2020

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65266	Long Jin, So-Ra Ko, Chang Soo Lee, Chi-Yong Ahn, Hee-Mock Oh, Hyung-Gwan Lee: Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water. IJSEM 67: 4443 - 4448 2017 ( DOI 10.1099/ijsem.0.002311 , PubMed 28954645 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Asprobacter aquaticus (KCTC 42356, JCM 30469, DRW22-8)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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