Name: Asprobacter aquaticus DRW22-8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158566

Type strain:

Species: Asprobacter aquaticus

Strain Designation: DRW22-8

Culture col. no.: KCTC 42356, JCM 30469

Strain history: <- Long Jin, KRIBB

NCBI tax ID(s): 1348470 (species)

Section

Asprobacter aquaticus DRW22-8 is an aerobe, Gram-negative, ovoid-shaped bacterium that forms circular colonies and was isolated from fresh water sample at a depth of 22 m.

colony-forming
Gram-negative
ovoid-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Caulobacterales*
	Family	*Hyphomonadaceae*
	Genus	*Asprobacter*
	Species	**Asprobacter aquaticus**
	Full Scientific Name (LPSN)	Asprobacter aquaticus Jin et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65266]	Gram stain	negative

[Ref.: #65266]	Cell shape	ovoid-shaped

[Ref.: #65266]	Motility	no

[Ref.: #65266]	Colony color	creamy-white
[Ref.: #65266]	Colony shape	circular
[Ref.: #65266]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65266]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #65266]

Culture medium growth

[Ref.: #65266]

Culture medium

tryptic soy agar

[Ref.: #65266]

Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65266]		growth	15-37 °C
[Ref.: #65266]		optimum	30 °C
[Ref.: #65266]	no	growth	8 °C
[Ref.: #65266]	no	growth	42 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #67771]		growth	30 °C

[Ref.: #65266]	Temperature range	mesophilic
[Ref.: #65266]	Temperature range	psychrophilic
[Ref.: #65266]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #65266]		growth	7
[Ref.: #65266]	no	growth	6
[Ref.: #65266]	no	growth	8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #65266]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65266]		NaCl		growth	1	%(w/v)
[Ref.: #65266]		NaCl	no	growth	2	%(w/v)

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65266]	2-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #65266]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65266]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65266]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65266]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #65266]	acetate ChEBI	-	assimilation
[Ref.: #65266]	adipate ChEBI	-	assimilation
[Ref.: #65266]	citrate ChEBI	-	assimilation
[Ref.: #65266]	D-glucose ChEBI	-	assimilation
[Ref.: #65266]	D-mannitol ChEBI	+	assimilation
[Ref.: #65266]	D-mannose ChEBI	-	assimilation
[Ref.: #65266]	D-ribose ChEBI	-	assimilation
[Ref.: #65266]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65266]	decanoate ChEBI	-	assimilation
[Ref.: #65266]	esculin ChEBI	+	hydrolysis
[Ref.: #65266]	gelatin ChEBI	-	hydrolysis
[Ref.: #65266]	gluconate ChEBI	-	assimilation
[Ref.: #65266]	glucose ChEBI	-	fermentation
[Ref.: #65266]	glycogen ChEBI	-	assimilation
[Ref.: #65266]	itaconate ChEBI	-	assimilation
[Ref.: #65266]	L-alanine ChEBI	-	assimilation
[Ref.: #65266]	L-arabinose ChEBI	-	assimilation
[Ref.: #65266]	L-fucose ChEBI	-	assimilation
[Ref.: #65266]	L-histidine ChEBI	-	assimilation
[Ref.: #65266]	L-proline ChEBI	+	assimilation
[Ref.: #65266]	L-rhamnose ChEBI	+	assimilation
[Ref.: #65266]	L-serine ChEBI	+	assimilation
[Ref.: #65266]	lactate ChEBI	-	assimilation
[Ref.: #65266]	malate ChEBI	-	assimilation
[Ref.: #65266]	malonate ChEBI	-	assimilation
[Ref.: #65266]	maltose ChEBI	-	assimilation
[Ref.: #65266]	melibiose ChEBI	-	assimilation
[Ref.: #65266]	myo-inositol ChEBI	-	assimilation
[Ref.: #65266]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65266]	nitrate ChEBI	-	reduction
[Ref.: #65266]	phenylacetate ChEBI	-	assimilation
[Ref.: #65266]	propionate ChEBI	-	assimilation
[Ref.: #65266]	salicin ChEBI	-	assimilation
[Ref.: #65266]	suberic acid ChEBI	-	assimilation
[Ref.: #65266]	sucrose ChEBI	-	assimilation
[Ref.: #65266]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65266]	ampicillin ChEBI	yes	50	µg/mL
[Ref.: #65266]	ampicillin ChEBI				yes	10	µg/mL
[Ref.: #65266]	ampicillin ChEBI				yes	30	µg/mL
[Ref.: #65266]	hygromycin ChEBI				yes	10	µg/mL
[Ref.: #65266]	hygromycin ChEBI				yes	30	µg/mL
[Ref.: #65266]	hygromycin ChEBI				yes	50	µg/mL
[Ref.: #65266]	imipenem ChEBI				yes	10	µg/mL
[Ref.: #65266]	imipenem ChEBI				yes	30	µg/mL
[Ref.: #65266]	imipenem ChEBI				yes	50	µg/mL
[Ref.: #65266]	penicillin g ChEBI	yes	30	µg/mL
[Ref.: #65266]	penicillin g ChEBI	yes	50	µg/mL
[Ref.: #65266]	penicillin g ChEBI				yes	10	µg/mL
[Ref.: #65266]	streptomycin ChEBI	yes	10	µg/mL
[Ref.: #65266]	streptomycin ChEBI	yes	30	µg/mL
[Ref.: #65266]	streptomycin ChEBI	yes	50	µg/mL
[Ref.: #65266]	tetracycline ChEBI	yes	30	µg/mL
[Ref.: #65266]	tetracycline ChEBI	yes	50	µg/mL
[Ref.: #65266]	tetracycline ChEBI				yes	10	µg/mL
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65266]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65266]	acid phosphatase	+	3.1.3.2
[Ref.: #65266]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65266]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65266]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65266]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65266]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65266]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65266]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65266]	beta-glucosidase	-	3.2.1.21
[Ref.: #65266]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65266]	catalase	-	1.11.1.6
[Ref.: #65266]	cystine arylamidase	-	3.4.11.3
[Ref.: #65266]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65266]	esterase (C 4)	+
[Ref.: #65266]	esterase Lipase (C 8)	+
[Ref.: #65266]	leucine arylamidase	+	3.4.11.1
[Ref.: #65266]	lipase (C 14)	-
[Ref.: #65266]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65266]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65266]	trypsin	-	3.4.21.4
[Ref.: #65266]	urease	-	3.5.1.5
[Ref.: #65266]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65266]	Sample type/isolated from	fresh water sample at a depth of 22 m
[Ref.: #65266]	Geographic location (country and/or sea, region)	Daechung Reservoir
[Ref.: #65266]	Country	Republic of Korea
[Ref.: #65266]	Country ISO 3 Code	KOR
[Ref.: #65266]	Continent	Asia
[Ref.: #65266]	Enrichment culture	modified 1/10 R2A agar
[Ref.: #65266]	Enrichment culture composition	(L-1: 0.05 g peptone, 0.05 g yeast extract, 0.05 g casamino acid, 0.05 g dextrose, 0.05 g soluble starch, 0.03 g K2HPO4 , 0.005 g MgSO4 , 0.03 g sodium pyruvate and 15 g agar)
[Ref.: #65266]	Enrichment culture duration	4 weeks
[Ref.: #65266]	Enrichment culture temperature	25 ̊C
[Ref.: #65266]	Isolation/enrichment procedure	A 100 µl subsample (10-5 or 10-6) of the suspended material was spread onto modified 1/10 R2A agar and incubated at room temperature (25°C) for 4 weeks.

[Ref.: #67770]	Sample type/isolated from	Fresh water taken at a depth of 22 m at Daechung Reservoir
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From microcystis culture
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF056993 (>99% sequence identity) for Asprobacter aquaticus subclade from Microbeatlas

Aquatic Samples	8470
Soil Samples	6086
Animal Samples	1245
Plant Samples	904
Total Samples	16705

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65266]	16S rRNA gene sequence	KF056993		GenBank

[Ref.: #65266]

GC-content

64.4 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65266]

Culture collection no.

KCTC 42356, JCM 30469

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water.	Jin L, Ko SR, Lee CS, Ahn CY, Oh HM, Lee HG	Int J Syst Evol Microbiol	10.1099/ijsem.0.002311	2017	*
Phylogeny	Pseudaquidulcibacter saccharophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.	Liu Y, Li S, Lin J, Liu S, Wei H, Dai J, Qiu D	Int J Syst Evol Microbiol	10.1099/ijsem.0.005264	2022	*
Phylogeny	Terricaulis silvestris gen. nov., sp. nov., a novel prosthecate, budding member of the family Caulobacteraceae isolated from forest soil.	Vieira S, Pascual J, Boedeker C, Geppert A, Riedel T, Rohde M, Overmann J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004367	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65266

Long Jin, So-Ra Ko, Chang Soo Lee, Chi-Yong Ahn, Hee-Mock Oh, Hyung-Gwan Lee: Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water. IJSEM 67: 4443 - 4448 2017 ( DOI 10.1099/ijsem.0.002311 , PubMed 28954645 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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