Name: Anaerobacillus alkaliphilus B16-10
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158471

Type strain:

Species: Anaerobacillus alkaliphilus

Strain Designation: B16-10

Culture col. no.: DSM 29790, NCAIM B.02608

Strain history: <- A. K. Borsodi, Eötvös Loránd Univ. Budapest, Hungary; B16-10

NCBI tax ID(s): 1548597 (species)

SI-ID 403610

DSM 29790

Special Collections

Other strains from Anaerobacillus alkaliphilus

Section

Anaerobacillus alkaliphilus B16-10 is a facultative anaerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from soda rhizosphere soil of Bolboschoenus maritimus.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Anaerobacillus*
	Species	**Anaerobacillus alkaliphilus**
	Full Scientific Name (LPSN)	Anaerobacillus alkaliphilus Borsodi et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67786]	Gram stain	positive

[Ref.: #67786]	Cell length	2-2.5 µm

[Ref.: #67786]	Cell width	0.5-0.8 µm

[Ref.: #67786]	Cell shape	rod-shaped

[Ref.: #67786]	Motility	yes
[Ref.: #67786]	Motility	yes

[Ref.: #67786]	Flagellum arrangement	subpolar
[Ref.: #67786]	Flagellum arrangement	monotrichous

[Ref.: #67786]	Colony color	creamish white
[Ref.: #67786]	Colony shape	circular
[Ref.: #67786]	Incubation period	1-2 days
[Ref.: #67786]	Cultivation medium used	nutrient medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65147]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #65147]

Culture medium growth

[Ref.: #65147]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65147]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65147]	growth	25 °C
[Ref.: #67786]	growth	10-40 °C
[Ref.: #67786]	optimum	25-28 °C

	pH	Kind of pH		pH
[Ref.: #67786]			optimum	9-10
[Ref.: #67786]			growth	7-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67786]

Oxygen tolerance

facultative anaerobe

[Ref.: #67786]	Spore description	Terminal, ellipsoidal endospores are formed in swollen sporangia.
[Ref.: #67786]	Type of spore	endospore
[Ref.: #67786]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %90.565

[Ref.: #67786]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67786]		NaCl		growth	0-7	%(w/v)
[Ref.: #67786]		NaCl		optimum	5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67786]	alanine ChEBI	-	carbon source
[Ref.: #67786]	asparagine ChEBI	-	carbon source
[Ref.: #67786]	casein	-	hydrolysis
[Ref.: #67786]	citrate ChEBI	-	assimilation
[Ref.: #67786]	D-fructose ChEBI	-	carbon source
[Ref.: #67786]	D-galactose ChEBI	-	carbon source
[Ref.: #67786]	D-glucose ChEBI	-	builds acid from
[Ref.: #67786]	D-glucose ChEBI	+	carbon source
[Ref.: #67786]	D-mannose ChEBI	-	carbon source
[Ref.: #67786]	D-rhamnose ChEBI	-	carbon source
[Ref.: #67786]	D-ribose ChEBI	-	carbon source
[Ref.: #67786]	D-xylose ChEBI	-	carbon source
[Ref.: #67786]	esculin ChEBI	+	hydrolysis
[Ref.: #67786]	galactitol ChEBI	-	carbon source
[Ref.: #67786]	gelatin ChEBI	+	hydrolysis
[Ref.: #67786]	glucose ChEBI	-	fermentation
[Ref.: #67786]	glycerol ChEBI	-	carbon source
[Ref.: #67786]	L-arabinose ChEBI	-	carbon source
[Ref.: #67786]	L-serine ChEBI	-	carbon source
[Ref.: #67786]	lactose ChEBI	-	carbon source
[Ref.: #67786]	maltose ChEBI	+	carbon source
[Ref.: #67786]	myo-inositol ChEBI	-	carbon source
[Ref.: #67786]	nitrate ChEBI	+	reduction
[Ref.: #67786]	sodium acetate ChEBI	-	carbon source
[Ref.: #67786]	sodium formate ChEBI	-	carbon source
[Ref.: #67786]	sodium pyruvate ChEBI	-	carbon source
[Ref.: #67786]	starch ChEBI	-	hydrolysis
[Ref.: #67786]	sucrose ChEBI	+	carbon source
[Ref.: #67786]	trehalose ChEBI	+	carbon source
[Ref.: #67786]	tween 80 ChEBI	+	hydrolysis
[Ref.: #67786]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #67786]	acetoin ChEBI	no
[Ref.: #67786]	hydrogen sulfide ChEBI	no
[Ref.: #67786]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #67786]		acetoin ChEBI	-
[Ref.: #67786]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #67786]	acid phosphatase	+	3.1.3.2
[Ref.: #67786]	alkaline phosphatase	+	3.1.3.1
[Ref.: #67786]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #67786]	alpha-fucosidase	-	3.2.1.51
[Ref.: #67786]	alpha-galactosidase	-	3.2.1.22
[Ref.: #67786]	alpha-glucosidase	+	3.2.1.20
[Ref.: #67786]	alpha-mannosidase	-	3.2.1.24
[Ref.: #67786]	beta-galactosidase	-	3.2.1.23
[Ref.: #67786]	beta-glucosidase	+	3.2.1.21
[Ref.: #67786]	beta-glucuronidase	-	3.2.1.31
[Ref.: #67786]	catalase	+	1.11.1.6
[Ref.: #67786]	cystine arylamidase	-	3.4.11.3
[Ref.: #67786]	cytochrome oxidase	-	1.9.3.1
[Ref.: #67786]	esterase (C 4)	+
[Ref.: #67786]	esterase Lipase (C 8)	+
[Ref.: #67786]	leucine arylamidase	+	3.4.11.1
[Ref.: #67786]	lipase (C 14)	-
[Ref.: #67786]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #67786]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #67786]	phosphatase	+
[Ref.: #67786]	trypsin	-	3.4.21.4
[Ref.: #67786]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65147]	Sample type/isolated from	soda rhizosphere soil of Bolboschoenus maritimus
[Ref.: #65147]	Geographic location (country and/or sea, region)	Kiskunság National Park (N 46.76767)
[Ref.: #65147]	Country	Hungary
[Ref.: #65147]	Country ISO 3 Code	HUN
[Ref.: #65147]	Continent	Europe
[Ref.: #65147]	Geographic location	46.7677°/19.1553°

[Ref.: #67786]	Sample type/isolated from	rhizosphere soil of Bolboschoenus maritimus
[Ref.: #67786]	Geographic location (country and/or sea, region)	shores of the Böddi-szék, Kiskunság National Park
[Ref.: #67786]	Country	Hungary
[Ref.: #67786]	Country ISO 3 Code	HUN
[Ref.: #67786]	Continent	Europe
[Ref.: #67786]	Geographic location	46.7676°/19.1553°
[Ref.: #67786]	Enrichment culture	modified Reasoner's 2A medium 830
[Ref.: #67786]	Enrichment culture composition	consisting of 0.5 g yeast extract, 0.5 g proteose peptone (Difco no. 3), 0.5 g casamino acids, 0.5 g glucose, 0.5 g soluble starch, 0.3 g Na-pyruvate, 0.3 g K2HPO4, 0.05 g MgSO4 x 7H2O and 15.0 g agar in 1000 ml distilled water, and supplemented with 50 g l-1 NaCl. The pH of the medium was adjusted to 9.0.
[Ref.: #67786]	Enrichment culture duration	6 days
[Ref.: #67786]	Enrichment culture temperature	28 ̊C
[Ref.: #67786]	Isolation/enrichment procedure	serial dilution
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65147]	Bacillus sp. B16-10 partial 16S rRNA gene, strain B16-10	LN610499	1483	GenBank ENA	1548597 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67786]	Anaerobacillus alkaliphilus B16-10	GCA_004116265	contig	GenBank ENA	1548597 tax ID

[Ref.: #65147]	GC-content	35.8 mol%
[Ref.: #67786]	GC-content	36.9 mol%	genome sequence analysis
[Ref.: #67786]	GC-content	35.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Anaerobacillus alkaliphilus B16-10 NCBI: GCA_004116265.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	74.531	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.992	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	69.774	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	90.565	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91.041	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.753	yes

Availability in culture collections External links

[Ref.: #65147]

Culture collection no.

DSM 29790, NCAIM B.02608

[Ref.: #111268]

SI-ID 403610

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium.	Borsodi AK, Aszalos JM, Bihari P, Nagy I, Schumann P, Sproer C, Kovacs AL, Boka K, Dobosy P, Ovari M, Szili-Kovacs T, Toth E	Int J Syst Evol Microbiol	10.1099/ijsem.0.003128	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65147

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29790

#67786

Andrea K. Borsodi, Júlia M. Aszalós, Péter Bihari, István Nagy, Peter Schumann, Cathrin Spröer, Attila L. Kovács, Károly Bóka, Péter Dobosy, Mihály Óvári, Tibor Szili-Kovács, Erika Tóth: Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium. IJSEM 69: 631 - 637 2019 ( DOI 10.1099/ijsem.0.003128 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#111268

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403610.1 )

* These data were automatically processed and therefore are not curated

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