Name: Micromonospora craniellae LHW63014
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158459

Type strain:

Species: Micromonospora craniellae

Strain Designation: LHW63014

Culture col. no.: DSM 106193, CCTCC AA 2018012

Strain history: <- L. Li, Shanghai Jiao Tong Univ., China; LHW63014

NCBI tax ID(s): 2294034 (species)

SI-ID 401557

DSM 106193

Section

Micromonospora craniellae LHW63014 is an aerobe, spore-forming, mesophilic bacterium that was isolated from marine sponge.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micromonosporales*
	Family	*Micromonosporaceae*
	Genus	*Micromonospora*
	Species	**Micromonospora craniellae**
	Full Scientific Name (LPSN)	Micromonospora craniellae Li et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67817]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #67817]	Motility	no

[Ref.: #69302]	Colony color	Daffodil yellow (1007), pastel yellow (1034)
[Ref.: #69302]	Cultivation medium used	ISP 4

[Ref.: #69302]	Colony color	Daffodil yellow (1007), pastel yellow (1034)
[Ref.: #69302]	Cultivation medium used	suter with tyrosine

[Ref.: #69302]	Colony color	Daffodil yellow (1007), pastel yellow (1034)
[Ref.: #69302]	Cultivation medium used	suter without tyrosine

[Ref.: #69302]	Colony color	Maize yellow (1006)
[Ref.: #69302]	Cultivation medium used	ISP 7

[Ref.: #69302]	Colony color	Signal orange (2010)
[Ref.: #69302]	Cultivation medium used	ISP 2

[Ref.: #69302]	Pigment production	no
[Ref.: #69302]	Pigment name	Melanin
[Ref.: #69302]	Pigment production	no
[Ref.: #69302]	Pigment name	soluble pigment

[Ref.: #67817]	Multicellular complex forming ability	yes
[Ref.: #67817]	Multicellular complex further description	forms well-developed branched substrate mycelium. Aerial hyphae is absent.

[Ref.: #69302]	Multicellular complex forming ability	no
[Ref.: #69302]	Multicellular complex name	Aerial mycelium
[Ref.: #69302]	Medium Name (multicellularity)	ISP 2

[Ref.: #69302]	Multicellular complex forming ability	no
[Ref.: #69302]	Multicellular complex name	Aerial mycelium
[Ref.: #69302]	Medium Name (multicellularity)	ISP 4

[Ref.: #69302]	Multicellular complex forming ability	no
[Ref.: #69302]	Multicellular complex name	Aerial mycelium
[Ref.: #69302]	Medium Name (multicellularity)	ISP 7

[Ref.: #69302]	Multicellular complex forming ability	no
[Ref.: #69302]	Multicellular complex name	Aerial mycelium
[Ref.: #69302]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69302]	Multicellular complex forming ability	no
[Ref.: #69302]	Multicellular complex name	Aerial mycelium
[Ref.: #69302]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #65135]

Caption:

Medium 83 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #65135]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #65135]			growth	28 °C
[Ref.: #67817]			growth	20-34 °C

[Ref.: #65135]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #67817]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67817]

Oxygen tolerance

aerobe

[Ref.: #67817]	Spore description	Single or clustered spherical spores (0.7 µm in diameter) with smooth surface are observed.
[Ref.: #67817]	Type of spore	spore
[Ref.: #67817]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67817]		NaCl		maximum	4	%
[Ref.: #69302]		NaCl		growth	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67817]	adenine ChEBI	-	nitrogen source
[Ref.: #69302]	arabinose ChEBI	+	growth
[Ref.: #67817]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #67817]	cellobiose ChEBI	-	carbon source
[Ref.: #69302]	cellulose ChEBI	-	growth
[Ref.: #67817]	D-arabinose ChEBI	-	carbon source
[Ref.: #67817]	D-fructose ChEBI	-	carbon source
[Ref.: #67817]	D-galactose ChEBI	+	carbon source
[Ref.: #67817]	D-glucose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	D-mannitol ChEBI	-	carbon source
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	D-mannose ChEBI	+	carbon source
[Ref.: #67817]	D-ribose ChEBI	-	carbon source
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	D-sorbitol ChEBI	-	carbon source
[Ref.: #67817]	D-xylose ChEBI	-	carbon source
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	esculin ChEBI	-	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69302]	fructose ChEBI	-	growth
[Ref.: #67817]	galactitol ChEBI	-	carbon source
[Ref.: #67817]	gelatin ChEBI	+	hydrolysis
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69302]	glucose ChEBI	+	growth
[Ref.: #67817]	glycerol ChEBI	-	carbon source
[Ref.: #67817]	glycine ChEBI	-	nitrogen source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	hydroxyproline ChEBI	+	nitrogen source
[Ref.: #67817]	hypoxanthine ChEBI	-	nitrogen source
[Ref.: #69302]	inositol ChEBI	+/-	growth
[Ref.: #67817]	L-alanine ChEBI	-	nitrogen source
[Ref.: #67817]	L-arginine ChEBI	+	nitrogen source
[Ref.: #67817]	L-asparagine ChEBI	-	nitrogen source
[Ref.: #67817]	L-aspartate ChEBI	-	nitrogen source
[Ref.: #67817]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #67817]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #67817]	L-glutamine ChEBI	-	nitrogen source
[Ref.: #67817]	L-histidine ChEBI	+	nitrogen source
[Ref.: #67817]	L-leucine ChEBI	-	nitrogen source
[Ref.: #67817]	L-lysine ChEBI	+	nitrogen source
[Ref.: #67817]	L-methionine ChEBI	-	nitrogen source
[Ref.: #67817]	L-phenylalanine ChEBI	-	nitrogen source
[Ref.: #67817]	L-proline ChEBI	-	nitrogen source
[Ref.: #67817]	L-rhamnose ChEBI	+	carbon source
[Ref.: #67817]	L-serine ChEBI	-	nitrogen source
[Ref.: #67817]	L-threonine ChEBI	-	nitrogen source
[Ref.: #67817]	L-tryptophan ChEBI	-	nitrogen source
[Ref.: #67817]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #67817]	L-valine ChEBI	-	nitrogen source
[Ref.: #67817]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	maltose ChEBI	-	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69302]	mannose ChEBI	-	growth
[Ref.: #67817]	melibiose ChEBI	+	carbon source
[Ref.: #67817]	milk	-	assimilation
[Ref.: #67817]	myo-inositol ChEBI	-	carbon source
[Ref.: #67817]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #67817]	raffinose ChEBI	-	carbon source
[Ref.: #69302]	raffinose ChEBI	+/-	growth
[Ref.: #69302]	rhamnose ChEBI	+	growth
[Ref.: #67817]	sodium acetate ChEBI	-	carbon source
[Ref.: #67817]	sodium citrate ChEBI	-	carbon source
[Ref.: #67817]	sodium pyruvate ChEBI	+	carbon source
[Ref.: #67817]	starch ChEBI	-	hydrolysis
[Ref.: #67817]	sucrose ChEBI	+	carbon source
[Ref.: #69302]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #67817]	trehalose ChEBI	+	carbon source
[Ref.: #67817]	tween 20 ChEBI	-	hydrolysis
[Ref.: #67817]	tween 40 ChEBI	+	hydrolysis
[Ref.: #67817]	tween 60 ChEBI	+	hydrolysis
[Ref.: #67817]	tween 80 ChEBI	-	hydrolysis
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #67817]	xanthine ChEBI	-	nitrogen source
[Ref.: #67817]	xylitol ChEBI	-	carbon source
[Ref.: #69302]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #67817]		hydrogen sulfide ChEBI	no
[Ref.: #67817]		melanin ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #67817]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #67817]

Fatty acid	Percentage
C16:0 iso	26.5
C15:0 iso	15.7
C17:1 w8c	12.1
C18:1 w9c	11.7
C17:0	6
C17:0 iso	5.2
C17:0 anteiso	4.6
C16:0	4
C18:0	3.8
10-methyl C16:0 or iso-C17:1w9c	3
C16:1 w7c / C16:1 w6c or C16:1 w6c / C16:1 w7c	2.7
C15:0 anteiso	1.3
C19:1 w9c / C19:1 w11c or C19:1 w9c / C19:1 w11c	0.8
C19:0	0.7
C18:0 iso	0.6
C17:1 anteiso w9c	0.3

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69302]	3795	-	+	-	-	-	-	-	+	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69302]	5979	not determinedn.d.	+/-	+	+/-	-	+	-	+/-	+	+	-	+/-	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65135]	Sample type/isolated from	marine sponge
[Ref.: #65135]	Geographic location (country and/or sea, region)	Xisha Islands, Hainan Province (16° 45' N, 112° 21' E)
[Ref.: #65135]	Country	China
[Ref.: #65135]	Country ISO 3 Code	CHN
[Ref.: #65135]	Continent	Asia
[Ref.: #65135]	Geographic location	16.7500°/112.3500°

[Ref.: #67817]	Sample type/isolated from	Craniella species sponge (coral reef at 20 m depth)
[Ref.: #67817]	Geographic location (country and/or sea, region)	Xisha Islands
[Ref.: #67817]	Country	China
[Ref.: #67817]	Country ISO 3 Code	CHN
[Ref.: #67817]	Continent	Asia
[Ref.: #67817]	Geographic location	16.7500°/112.3500°
[Ref.: #67817]	Enrichment culture	Streptomyces Isolation Medium
[Ref.: #67817]	Enrichment culture composition	3 % unrefined sea salt (w/v), 50 mg l-1 cycloheximide and 25 mg l-1 nalidixic acid
[Ref.: #67817]	Enrichment culture duration	4-12 weeks
[Ref.: #67817]	Enrichment culture temperature	28 ̊C
[Ref.: #67817]	Isolation/enrichment procedure	Marine sponge samples were collected by surface-supplied diving and delivered in ice. After being washed in sterile artificial sea water three times, the sponge tissues were cut, ground and spread on Streptomyces Isolation Medium. The plates were incubated at 28°C for 4-12 weeks.
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Porifera (Sponges)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65135]	Jishengella sp. strain LHW63014 16S ribosomal RNA gene, partial sequence	MG200153	1479	ENA	2294034 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67817]	Micromonospora craniellae LHW63014	GCA_003419945	contig	GenBank	2294034 tax ID
[Ref.: #66792]	Micromonospora craniellae LHW63014	GCA_014764405	complete	GenBank	2294034 tax ID	*
[Ref.: #66792]	Micromonospora craniellae strain LHW63014	2294034.4	complete	PATRIC	2294034 tax ID	*
[Ref.: #66792]	Micromonospora craniellae LHW63014	2866394982	draft	JGI IMG/M	2294034 tax ID	*

[Ref.: #65135]	GC-content	71.0 mol%
[Ref.: #67817]	GC-content	71 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Micromonospora craniellae LHW63014 NCBI: GCA_014764405.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Micromonospora craniellae LHW63014 NCBI: GCA_014764405.1
[Ref.: #69480]	flagellated	no	97.538	yes
[Ref.: #69480]	gram-positive	yes	91.993	yes
[Ref.: #69480]	anaerobic	no	99.163	yes
[Ref.: #69480]	aerobic	yes	93.653	no
[Ref.: #69480]	halophile	no	94.13	yes
[Ref.: #69480]	spore-forming	yes	94.049	no
[Ref.: #69480]	glucose-util	yes	87.537	yes
[Ref.: #69480]	thermophile	no	98.175	yes
[Ref.: #69480]	motile	no	92.276	yes
[Ref.: #69480]	glucose-ferment	no	92.889	yes

Availability in culture collections External links

[Ref.: #65135]

Culture collection no.

DSM 106193, CCTCC AA 2018012

[Ref.: #111257]

SI-ID 401557

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Micromonospora craniellae sp. nov., isolated from a marine sponge, and reclassification of Jishengella endophytica as Micromonospora endophytica comb. nov.	Li L, Zhu HR, Xu QH, Lin HW, Lu YH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003209	2019	*
Phylogeny	Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake.	Villalobos AS, Wiese J, Borchert E, Rahn T, Slaby BM, Steiner LX, Kunzel S, Dorador C, Imhoff JF	Int J Syst Evol Microbiol	10.1099/ijsem.0.005109	2021	*
Genetics	Complete genome sequence of Micromonospora craniellae LHW63014(T), a potential metal ion-chelating agent producer.	Yang X, Zhu ZH, Ji X, Liu ZM, Zhang H, Wei B	Mar Genomics	10.1016/j.margen.2020.100830	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65135

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106193

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67817

Lei Li, Hong-rui Zhu, Qi-hang Xu, Hou-wen Lin, Yan-hua Lu: Micromonospora craniellae sp. nov., isolated from a marine sponge, and reclassification of Jishengella endophytica as Micromonospora endophytica comb. nov.. IJSEM 69: 715 - 720 2019 ( DOI 10.1099/ijsem.0.003209 )

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69302

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111257

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401557.1 )

* These data were automatically processed and therefore are not curated

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