Micromonospora craniellae (DSM 106193, CCTCC AA 2018012, LHW63014)

Name: Micromonospora craniellae (DSM 106193, CCTCC AA 2018012, LHW63014)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158459

Type strain

Strain Designation LHW63014

Culture col. no. DSM 106193 CCTCC AA 2018012 LHW63014

NCBI tax ID(s) 2294034

Special Collections DSMZ Wink compendium Literature strains

History DSMZ <- L. Li, Shanghai Jiao Tong Univ., China; LHW63014

Links

Micromonospora craniellae LHW63014 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from marine sponge.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 401557

DSM 106193

@ref 20215

Last LPSN update 2026-05-29 08:43:58

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora craniellae

Full scientific name Micromonospora craniellae Li et al. 2019

Morphology

Cell morphology

@ref	Gram stain	Confidence
67817	positive
125438	positive	90.646
125439		97.017
125439	positive	99.818

Colony morphology

@ref	Colony color	Medium used
69302	Daffodil yellow (1007), pastel yellow (1034)	ISP 4
69302	Daffodil yellow (1007), pastel yellow (1034)	suter with tyrosine
69302	Daffodil yellow (1007), pastel yellow (1034)	suter without tyrosine
69302	Maize yellow (1006)	ISP 7
69302	Signal orange (2010)	ISP 2

Multicellular morphology

@ref	Complex name	Medium name	Further description
67817			forms well-developed branched substrate mycelium. Aerial hyphae is absent.
69302	Aerial mycelium	ISP 2
69302	Aerial mycelium	ISP 4
69302	Aerial mycelium	ISP 7
69302	Aerial mycelium	suter with tyrosine
69302	Aerial mycelium	suter without tyrosine

Pigmentation

@ref	Production	Name
69302		melanin pigment
69302		soluble pigment

Multimedia

@ref:	65135
multimedia content:	DSM_106193.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_106193.jpg
caption:	Medium 83 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65135	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
65135	CZAPEK PEPTONE AGAR (DSMZ Medium 83)		Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
65135	positive	growth	28
67817	positive	growth	20-34

Culture pH

@ref	Ability	Type	PH	PH range
67817	positive	growth	6-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
67817	aerobe
125439	obligate aerobe	98.674

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
67817	Single or clustered spherical spores (0.7 µm in diameter) with smooth surface are observed.	spore
125439				94.487

Halophily

@ref	Salt	Growth	Tested relation	Concentration
67817	NaCl	positive	maximum	4 %
69302	NaCl	positive	growth	0 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
67817	16708 ChEBI	adenine	-	nitrogen source
69302	22599 ChEBI	arabinose	+	growth
67817	85146 ChEBI	carboxymethylcellulose	+	hydrolysis
67817	17057 ChEBI	cellobiose	-	carbon source
69302	62968 ChEBI	cellulose	-	growth
67817	17108 ChEBI	D-arabinose	-	carbon source
67817	15824 ChEBI	D-fructose	-	carbon source
67817	12936 ChEBI	D-galactose	+	carbon source
67817	17634 ChEBI	D-glucose	+	carbon source
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
67817	16899 ChEBI	D-mannitol	-	carbon source
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
67817	16024 ChEBI	D-mannose	+	carbon source
67817	16988 ChEBI	D-ribose	-	carbon source
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
67817	17924 ChEBI	D-sorbitol	-	carbon source
67817	65327 ChEBI	D-xylose	-	carbon source
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
67817	4853 ChEBI	esculin	-	hydrolysis
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
69302	28757 ChEBI	fructose	-	growth
67817	16813 ChEBI	galactitol	-	carbon source
67817	5291 ChEBI	gelatin	+	hydrolysis
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
69302	17234 ChEBI	glucose	+	growth
67817	17754 ChEBI	glycerol	-	carbon source
67817	15428 ChEBI	glycine	-	nitrogen source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
67817	24741 ChEBI	hydroxyproline	+	nitrogen source
67817	17368 ChEBI	hypoxanthine	-	nitrogen source
67817	16977 ChEBI	L-alanine	-	nitrogen source
67817	16467 ChEBI	L-arginine	+	nitrogen source
67817	17196 ChEBI	L-asparagine	-	nitrogen source
67817	29991 ChEBI	L-aspartate	-	nitrogen source
67817	17561 ChEBI	L-cysteine	-	nitrogen source
67817	29988 ChEBI	L-glutamate	-	nitrogen source
67817	18050 ChEBI	L-glutamine	-	nitrogen source
67817	15971 ChEBI	L-histidine	+	nitrogen source
67817	15603 ChEBI	L-leucine	-	nitrogen source
67817	18019 ChEBI	L-lysine	+	nitrogen source
67817	16643 ChEBI	L-methionine	-	nitrogen source
67817	17295 ChEBI	L-phenylalanine	-	nitrogen source
67817	17203 ChEBI	L-proline	-	nitrogen source
67817	62345 ChEBI	L-rhamnose	+	carbon source
67817	17115 ChEBI	L-serine	-	nitrogen source
67817	16857 ChEBI	L-threonine	-	nitrogen source
67817	16828 ChEBI	L-tryptophan	-	nitrogen source
67817	17895 ChEBI	L-tyrosine	-	nitrogen source
67817	16414 ChEBI	L-valine	-	nitrogen source
67817	17716 ChEBI	lactose	+	carbon source
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
67817	17306 ChEBI	maltose	-	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
69302	37684 ChEBI	mannose	-	growth
67817	28053 ChEBI	melibiose	+	carbon source
67817		milk	-	assimilation
67817	17268 ChEBI	myo-inositol	-	carbon source
69302	17268 ChEBI	myo-inositol	+/-	growth
67817	17632 ChEBI	nitrate	+	reduction
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
67817	16634 ChEBI	raffinose	-	carbon source
69302	16634 ChEBI	raffinose	+/-	growth
69302	26546 ChEBI	rhamnose	+	growth
67817	32954 ChEBI	sodium acetate	-	carbon source
67817	53258 ChEBI	sodium citrate	-	carbon source
67817	50144 ChEBI	sodium pyruvate	+	carbon source
67817	28017 ChEBI	starch	-	hydrolysis
67817	17992 ChEBI	sucrose	+	carbon source
69302	17992 ChEBI	sucrose	+	growth
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
67817	27082 ChEBI	trehalose	+	carbon source
67817	53424 ChEBI	tween 20	-	hydrolysis
67817	53423 ChEBI	tween 40	+	hydrolysis
67817	53425 ChEBI	tween 60	+	hydrolysis
67817	53426 ChEBI	tween 80	-	hydrolysis
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
67817	15318 ChEBI	xanthine	-	nitrogen source
67817	17151 ChEBI	xylitol	-	carbon source
69302	18222 ChEBI	xylose	-	growth

Metabolite production

@ref	Chebi-ID	Metabolite	Production
67817	16136 ChEBI	hydrogen sulfide
67817	89634 ChEBI	melanin

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
67817	catalase	+	1.11.1.6
68382	esterase (C 4)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

67817

Fatty acid	Percentage
C16:0 iso	26.5
C15:0 iso	15.7
C17:1 w8c	12.1
C18:1 w9c	11.7
C17:0	6
C17:0 iso	5.2
C17:0 anteiso	4.6
C16:0	4
C18:0	3.8
10-methyl C16:0 or iso-C17:1w9c	3
C16:1 w7c / C16:1 w6c or C16:1 w6c / C16:1 w7c	2.7
C15:0 anteiso	1.3
C19:1 w9c / C19:1 w11c or C19:1 w9c / C19:1 w11c	0.8
C19:0	0.7
C18:0 iso	0.6
C17:1 anteiso w9c	0.3

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69302	-	+	-	-	-	-	-	+	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69302	not determinedn.d.	+/-	+	+/-	-	+	-	+/-	+	+	-	+/-	-	-	-	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Porifera (Sponges)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
65135	marine sponge	Xisha Islands, Hainan Province (16° 45' N, 112° 21' E)	China	CHN	Asia	16.75	112.35 16.75/112.35
67817	Craniella species sponge (coral reef at 20 m depth)	Xisha Islands	China	CHN	Asia	16.75	112.35 16.75/112.35	Streptomyces Isolation Medium	3 % unrefined sea salt (w/v), 50 mg l-1 cycloheximide and 25 mg l-1 nalidixic acid	4-12 weeks	28	Marine sponge samples were collected by surface-supplied diving and delivered in ice. After being washed in sterile artificial sea water three times, the sponge tissues were cut, ground and spread on Streptomyces Isolation Medium. The plates were incubated at 28°C for 4-12 weeks.

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1476440v1 assembly for Micromonospora craniellae LHW63014	complete	GCA_014764405	2294034.4	8114287368	2294034	91.62
67817	ASM341994v1 assembly for Micromonospora craniellae LHW63014	contig	GCA_003419945	2294034.3	2866394982	2294034	37.26

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
65135	Micromonospora craniellae strain LHW63014 16S ribosomal RNA gene, partial sequence	MG200153	1479		2294034

GC content

@ref	GC-content (mol%)	Method
65135	71.0
67817	71	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1476440v1 assembly for Micromonospora craniellae LHW63014
BV-BRC: 2294034.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.67	no
125439	gram_stain	BacteriaNetⓘ	positive	99.82	no
125439	spore_formation	BacteriaNetⓘ	yes	94.49	no
125439	motility	BacteriaNetⓘ	no	97.02	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Micromonospora craniellae LHW63014
NCBI: GCA_014764405.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.65	yes
125438	anaerobic	anaerobicⓘ	no	93.28	yes
125438	aerobic	aerobicⓘ	yes	86.30	yes
125438	spore-forming	spore-formingⓘ	yes	89.16	no
125438	thermophilic	thermophileⓘ	no	90.00	no
125438	flagellated	motile2+ⓘ	no	89.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Micromonospora craniellae LHW63014(T), a potential metal ion-chelating agent producer.	Yang X, Zhu ZH, Ji X, Liu ZM, Zhang H, Wei B	Mar Genomics	10.1016/j.margen.2020.100830	2020
Phylogeny	Micromonospora craniellae sp. nov., isolated from a marine sponge, and reclassification of Jishengella endophytica as Micromonospora endophytica comb. nov.	Li L, Zhu HR, Xu QH, Lin HW, Lu YH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003209	2019
Phylogeny	Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake.	Villalobos AS, Wiese J, Borchert E, Rahn T, Slaby BM, Steiner LX, Kunzel S, Dorador C, Imhoff JF	Int J Syst Evol Microbiol	10.1099/ijsem.0.005109	2021

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65135	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106193
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67817	Lei Li, Hong-rui Zhu, Qi-hang Xu, Hou-wen Lin, Yan-hua Lu: Micromonospora craniellae sp. nov., isolated from a marine sponge, and reclassification of Jishengella endophytica as Micromonospora endophytica comb. nov.. IJSEM 69: 715 - 720 2019 ( DOI 10.1099/ijsem.0.003209 )
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69302	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Micromonospora craniellae (DSM 106193, CCTCC AA 2018012, LHW63014)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_014764405

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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