Name: Kribbella monticola NEAU-SW521
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158430

Type strain:

Species: Kribbella monticola

Strain Designation: NEAU-SW521

Culture col. no.: DSM 105770, CGMCC 4.7465

Strain history: <- J. Zhao, Northeast Agricultural Univ., Harbin, China <- W. Song; NEAU-SW521

NCBI tax ID(s): 2185285 (species)

SI-ID 401634

DSM 105770

Section

Kribbella monticola NEAU-SW521 is an aerobe, mesophilic, Gram-positive bacterium that builds a substrate mycelium and aerial mycelium and was isolated from soil.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Kribbellaceae*
	Genus	*Kribbella*
	Species	**Kribbella monticola**
	Full Scientific Name (LPSN)	Kribbella monticola Song et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66722]	Gram stain	positive

[Ref.: #66722]

Pigment production

[Ref.: #66722]	Multicellular complex forming ability	yes
[Ref.: #66722]	Multicellular complex name	substrate mycelium and aerial mycelium
[Ref.: #66722]	Medium Name (multicellularity)	ISP
[Ref.: #66722]	Multicellular complex further description	differentiates into rod-shaped elements

Information on culture and growth conditions Culture and growth conditions

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Bennett's and Czapek's agar media

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Culture medium growth

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Culture medium

ISP 1

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Culture medium growth

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 4

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Culture medium growth

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Culture medium

ISP 5

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Culture medium growth

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Culture medium

ISP 6

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Culture medium growth

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Culture medium

ISP 7

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #65106]	growth	28 °C
[Ref.: #66722]	optimum	28 °C
[Ref.: #66722]	growth	15-37 °C

[Ref.: #65106]	Temperature range	mesophilic
[Ref.: #66722]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66722]			optimum	7
[Ref.: #66722]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66722]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66722]		NaCl		growth	2	%(w/v)

[Ref.: #66722]	Observation	The cell wall contains LL-diaminopimelic acid and the whole-cell hydrolysates are ribose, glucose and galactose.
[Ref.: #66722]	Observation	The polar lipids contain diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
[Ref.: #66722]	Observation	The predominant menaquinone is MK-9 (H4).

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66722]	creatine ChEBI	-	nitrogen source
[Ref.: #66722]	D-fructose ChEBI	+	carbon source
[Ref.: #66722]	D-galactose ChEBI	+	carbon source
[Ref.: #66722]	D-glucose ChEBI	+	carbon source
[Ref.: #66722]	D-mannitol ChEBI	+	carbon source
[Ref.: #66722]	D-mannose ChEBI	+	carbon source
[Ref.: #66722]	D-ribose ChEBI	-	carbon source
[Ref.: #66722]	D-sorbitol ChEBI	-	carbon source
[Ref.: #66722]	D-xylose ChEBI	-	carbon source
[Ref.: #66722]	esculin ChEBI	+	hydrolysis
[Ref.: #66722]	glycine ChEBI	+	nitrogen source
[Ref.: #66722]	hydrogen sulfide ChEBI	-	assimilation
[Ref.: #66722]	L-alanine ChEBI	+	nitrogen source
[Ref.: #66722]	L-arabinose ChEBI	-	carbon source
[Ref.: #66722]	L-arginine ChEBI	+	nitrogen source
[Ref.: #66722]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #66722]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #66722]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #66722]	L-glutamine ChEBI	+	nitrogen source
[Ref.: #66722]	L-proline ChEBI	+	nitrogen source
[Ref.: #66722]	L-rhamnose ChEBI	+	carbon source
[Ref.: #66722]	L-serine ChEBI	+	nitrogen source
[Ref.: #66722]	L-threonine ChEBI	+	nitrogen source
[Ref.: #66722]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #66722]	lactose ChEBI	+	carbon source
[Ref.: #66722]	maltose ChEBI	+	carbon source
[Ref.: #66722]	myo-inositol ChEBI	+	carbon source
[Ref.: #66722]	nitrate ChEBI	-	reduction
[Ref.: #66722]	raffinose ChEBI	+	carbon source
[Ref.: #66722]	starch ChEBI	+	hydrolysis
[Ref.: #66722]	sucrose ChEBI	+	carbon source
[Ref.: #66722]	tween 20 ChEBI	-	reduction
[Ref.: #66722]	tween 40 ChEBI	-	reduction
[Ref.: #66722]	tween 80 ChEBI	-	reduction
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66722]	cellulase	-	3.2.1.4
[Ref.: #66722]	gelatinase	+
[Ref.: #66722]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66722]

Fatty acid	Percentage
C16:0 iso	30.3
C15:0 anteiso	22.5
C14:0 iso	8.9
C15:0	8.8
C17:0 iso	8
C17:0 anteiso	7.3
C16:0	5.3
C17:0	3.3
C18:0	2.7
C14:0	1.2
C17:1 w7c	1
C16:1 w7c	0.3
10-methyl C18:0	0.3
C13:0	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65106]	Sample type/isolated from	soil
[Ref.: #65106]	Geographic location (country and/or sea, region)	Mount Xianglu, Bin County, Heilongjiang province
[Ref.: #65106]	Country	China
[Ref.: #65106]	Country ISO 3 Code	CHN
[Ref.: #65106]	Continent	Asia

[Ref.: #66722]	Sample type/isolated from	isolated from soil
[Ref.: #66722]	Geographic location (country and/or sea, region)	Xianglu Mountain, Binxin, Heilongjiang Province
[Ref.: #66722]	Country	China
[Ref.: #66722]	Country ISO 3 Code	CHN
[Ref.: #66722]	Continent	Asia
[Ref.: #66722]	Enrichment culture	Gause's synthetic agar no 1
[Ref.: #66722]	Enrichment culture composition	supplemented with cycloheximide (50 mg/L) and nalidixic acid (50 mg/L).
[Ref.: #66722]	Enrichment culture duration	15 days
[Ref.: #66722]	Enrichment culture temperature	28 ̊C
[Ref.: #66722]	Isolation/enrichment procedure	The soil sample was air-dried at room temperature for 14 days before isolation of actinobacteria. After drying, the soil sample (5 g) was suspended in distilled water (45 ml) then the soil suspension was incubated at 28 C and 250 r.p.m. on a rotary shaker for 30 min. Subsequently, a 200 µl sample of the suspension was spread on a plate of Gause's synthetic agar no 1
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66722]	Kribbella sp. strain NEAU-SW521 16S ribosomal RNA gene, partial sequence	MG729590	1510	ENA	1871183 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Kribbella monticola NEAU-SW521	GCA_003261635	contig	GenBank	2185285 tax ID	*
[Ref.: #66792]	Kribbella monticola NEAU-SW521	2838804460	draft	JGI IMG/M	2185285 tax ID	*

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GC-content

67.8 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65106]

Culture collection no.

DSM 105770, CGMCC 4.7465

[Ref.: #111231]

SI-ID 401634

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kribbella monticola sp. nov., a novel actinomycete isolated from soil.	Song W, Duan L, Zhao J, Jiang S, Guo X, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.003007	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65106

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105770

#66722

Wei Song, Liping Duan, Junwei Zhao, Shanwen Jiang, Xiaowei Guo, Wensheng Xiang and Xiangjing Wang: Kribbella monticola sp. nov., a novel actinomycete isolated from soil. IJSEM 68: 3441 - 3446 2018 ( DOI 10.1099/ijsem.0.003007 , PubMed 30383522 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#111231

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401634.1 )

* These data were automatically processed and therefore are not curated

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