Strain identifier

BacDive ID: 158430

Type strain: Yes

Species: Kribbella monticola

Strain Designation: NEAU-SW521

Strain history: <- J. Zhao, Northeast Agricultural Univ., Harbin, China <- W. Song; NEAU-SW521

NCBI tax ID(s): 2185285 (species)

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General

@ref: 65106

BacDive-ID: 158430

DSM-Number: 105770

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Kribbella monticola NEAU-SW521 is an aerobe, mesophilic, Gram-positive bacterium that builds a substrate mycelium and aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 2185285
  • Matching level: species

strain history

  • @ref: 65106
  • history: <- J. Zhao, Northeast Agricultural Univ., Harbin, China <- W. Song; NEAU-SW521

doi: 10.13145/bacdive158430.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Kribbellaceae
  • genus: Kribbella
  • species: Kribbella monticola
  • full scientific name: Kribbella monticola Song et al. 2018

@ref: 65106

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Kribbellaceae

genus: Kribbella

species: Kribbella monticola

full scientific name: Kribbella monticola Song et al. 2018

strain designation: NEAU-SW521

type strain: yes

Morphology

cell morphology

  • @ref: 66722
  • gram stain: positive

multicellular morphology

  • @ref: 66722
  • forms multicellular complex: yes
  • complex name: substrate mycelium and aerial mycelium
  • medium name: ISP
  • further description: differentiates into rod-shaped elements

pigmentation

  • @ref: 66722
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65106R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
66722Bennett's and Czapek's agar mediayes
66722ISP 1yes
66722ISP 2yes
66722ISP 3yes
66722ISP 4yes
66722ISP 5yes
66722ISP 6yes
66722ISP 7yes
66722Nutrient agar (NA)yes

culture temp

@refgrowthtypetemperaturerange
65106positivegrowth28mesophilic
66722positiveoptimum28mesophilic
66722positivegrowth15-37

culture pH

@refabilitytypepHPH range
66722positiveoptimum7
66722positivegrowth5-9alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66722
  • oxygen tolerance: aerobe

halophily

  • @ref: 66722
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 2 %(w/v)

observation

@refobservation
66722The cell wall contains LL-diaminopimelic acid and the whole-cell hydrolysates are ribose, glucose and galactose.
66722The polar lipids contain diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
66722The predominant menaquinone is MK-9 (H4).

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6672216136hydrogen sulfide-assimilation
6672216988D-ribose-carbon source
6672217924D-sorbitol-carbon source
6672230849L-arabinose-carbon source
6672265327D-xylose-carbon source
6672212936D-galactose+carbon source
6672215824D-fructose+carbon source
6672216024D-mannose+carbon source
6672216634raffinose+carbon source
6672216899D-mannitol+carbon source
6672217268myo-inositol+carbon source
6672217306maltose+carbon source
6672217634D-glucose+carbon source
6672217716lactose+carbon source
6672217992sucrose+carbon source
6672262345L-rhamnose+carbon source
667224853esculin+hydrolysis
6672228017starch+hydrolysis
6672216919creatine-nitrogen source
6672217895L-tyrosine-nitrogen source
6672215428glycine+nitrogen source
6672216467L-arginine+nitrogen source
6672216857L-threonine+nitrogen source
6672216977L-alanine+nitrogen source
6672217115L-serine+nitrogen source
6672217196L-asparagine+nitrogen source
6672217203L-proline+nitrogen source
6672218050L-glutamine+nitrogen source
6672229985L-glutamate+nitrogen source
6672229991L-aspartate+nitrogen source
6672217632nitrate-reduction
6672253423tween 40-reduction
6672253424tween 20-reduction
6672253426tween 80-reduction

enzymes

@refvalueactivityec
66722cellulase-3.2.1.4
66722urease-3.5.1.5
66722gelatinase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66722C13:00.1
    6672210-methyl C18:00.3
    66722C16:1 w7c0.3
    66722C17:1 w7c1
    66722C14:01.2
    66722C18:02.7
    66722C15:0 anteiso22.5
    66722C17:03.3
    66722C16:0 iso30.3
    66722C16:05.3
    66722C17:0 anteiso7.3
    66722C17:0 iso8
    66722C15:08.8
    66722C14:0 iso8.9
  • type of FA analysis: whole cell analysis
  • incubation medium: GY broth
  • incubation temperature: 28
  • incubation time: 7
  • library/peak naming table: NIST
  • system: MIS MIDI
  • method/protocol: Xiang et al. 2011

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65106soilMount Xianglu, Bin County, Heilongjiang provinceChinaCHNAsia
66722isolated from soilXianglu Mountain, Binxin, Heilongjiang ProvinceChinaCHNAsiaGause's synthetic agar no 1supplemented with cycloheximide (50 mg/L) and nalidixic acid (50 mg/L).15 days28The soil sample was air-dried at room temperature for 14 days before isolation of actinobacteria. After drying, the soil sample (5 g) was suspended in distilled water (45 ml) then the soil suspension was incubated at 28 C and 250 r.p.m. on a rotary shaker for 30 min. Subsequently, a 200 µl sample of the suspension was spread on a plate of Gause's synthetic agar no 1

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 66722
  • description: Kribbella sp. strain NEAU-SW521 16S ribosomal RNA gene, partial sequence
  • accession: MG729590
  • length: 1510
  • database: ena
  • NCBI tax ID: 1871183

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kribbella monticola NEAU-SW521GCA_003261635contigncbi2185285
66792Kribbella monticola NEAU-SW5212838804460draftimg2185285

GC content

  • @ref: 65106
  • GC-content: 67.8
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 65106

culture collection no.: DSM 105770, CGMCC 4.7465

straininfo link

  • @ref: 111231
  • straininfo: 401634

literature

  • topic: Phylogeny
  • Pubmed-ID: 30383522
  • title: Kribbella monticola sp. nov., a novel actinomycete isolated from soil.
  • authors: Song W, Duan L, Zhao J, Jiang S, Guo X, Xiang W, Wang X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003007
  • year: 2018
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65106Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105770Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105770)
66722Wei Song, Liping Duan, Junwei Zhao, Shanwen Jiang, Xiaowei Guo, Wensheng Xiang and Xiangjing WangKribbella monticola sp. nov., a novel actinomycete isolated from soil10.1099/ijsem.0.003007IJSEM 68: 3441-3446 201830383522
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
111231Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401634.1