Name: Actinomadura rhizosphaerae SDA37
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158374

Type strain:

Species: Actinomadura rhizosphaerae

Strain Designation: SDA37

Culture col. no.: DSM 108648, KCTC 39965, NBRC 112909, TISTR 2523

Strain history: <- Somboon Tanasupawat, Chulalongkorn Univ.

NCBI tax ID(s): 2291546 (species)

SI-ID 399841

DSM 108648

Section

Actinomadura rhizosphaerae SDA37 is an aerobe, spore-forming, mesophilic bacterium that was isolated from rhizosphere soil of Azadirachta indica.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura rhizosphaerae**
	Full Scientific Name (LPSN)	Actinomadura rhizosphaerae Malisorn et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66669]	Gram stain	positive

[Ref.: #66669]	Motility	no

[Ref.: #66669]	Colony color	yellowish brown to greyish yellow
[Ref.: #66669]	Cultivation medium used	agar medium

[Ref.: #66669]	Multicellular complex forming ability	yes
[Ref.: #66669]	Multicellular complex color	white
[Ref.: #66669]	Medium Name (multicellularity)	ISP3, ISP4
[Ref.: #66669]	Multicellular complex further description	aerial mycelium

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

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Medium recipe at MediaDive

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium

BHI MEDIUM (DSMZ Medium 215)

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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R2A MEDIUM (DSMZ Medium 830)

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Culture medium

BENNETT'S AGAR (DSMZ Medium 548)

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Culture medium growth

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Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

agar medium

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65050]		growth	28 °C
[Ref.: #66669]	no	growth	45 °C
[Ref.: #66669]		growth	20-40 °C
[Ref.: #66669]		optimum	30-37 °C
[Ref.: #67771]		growth	30 °C

[Ref.: #65050]	Temperature range	mesophilic
[Ref.: #66669]	Temperature range	thermophilic
[Ref.: #66669]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66669]			optimum	6-7.5
[Ref.: #66669]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66669]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #66669]	Spore description	The spore is 0.4-0.5 x 0.9-1.2 µm in size and straight or flexuous chains of smooth-ornamented spores.
[Ref.: #66669]	Type of spore	spore
[Ref.: #66669]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66669]		NaCl		growth	0-4	%(w/v)

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Observation

Produces brown soluble pigment on ISP2, ISP6 and ISP7 media, and yellow soluble pigment on ISP3. No pigment is observed on ISP4 and ISP5.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66669]	cellobiose ChEBI	+	carbon source
[Ref.: #66669]	cellulose ChEBI	-	carbon source
[Ref.: #66669]	D-fructose ChEBI	+	carbon source
[Ref.: #66669]	D-galactose ChEBI	-	carbon source
[Ref.: #66669]	D-mannitol ChEBI	-	carbon source
[Ref.: #66669]	D-mannose ChEBI	-	carbon source
[Ref.: #66669]	D-xylose ChEBI	+	carbon source
[Ref.: #66669]	gelatin ChEBI	+	hydrolysis
[Ref.: #66669]	glycerol ChEBI	-	carbon source
[Ref.: #66669]	L-arabinose ChEBI	+	carbon source
[Ref.: #66669]	L-rhamnose ChEBI	-	carbon source
[Ref.: #66669]	melibiose ChEBI	-	carbon source
[Ref.: #66669]	myo-inositol ChEBI	-	carbon source
[Ref.: #66669]	nitrate ChEBI	+	reduction
[Ref.: #66669]	raffinose ChEBI	-	carbon source
[Ref.: #66669]	starch ChEBI	-	hydrolysis
[Ref.: #66669]	sucrose ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66669]	acid phosphatase	+	3.1.3.2
[Ref.: #66669]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66669]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #66669]	alpha-fucosidase	+	3.2.1.51
[Ref.: #66669]	alpha-galactosidase	+	3.2.1.22
[Ref.: #66669]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66669]	alpha-mannosidase	+	3.2.1.24
[Ref.: #66669]	beta-galactosidase	+	3.2.1.23
[Ref.: #66669]	beta-glucosidase	-	3.2.1.21
[Ref.: #66669]	beta-glucuronidase	+	3.2.1.31
[Ref.: #66669]	cystine arylamidase	+	3.4.11.3
[Ref.: #66669]	esterase (C 4)	+
[Ref.: #66669]	esterase Lipase (C 8)	+
[Ref.: #66669]	leucine arylamidase	-	3.4.11.1
[Ref.: #66669]	lipase (C 14)	+
[Ref.: #66669]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66669]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66669]	trypsin	+	3.4.21.4
[Ref.: #66669]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65050]	Sample type/isolated from	rhizosphere soil of Azadirachta indica
[Ref.: #65050]	Geographic location (country and/or sea, region)	Udon Thani Province
[Ref.: #65050]	Country	Thailand
[Ref.: #65050]	Country ISO 3 Code	THA
[Ref.: #65050]	Continent	Asia

[Ref.: #66669]	Sample type/isolated from	rhizosphere soil of the plant Azadirachta indica
[Ref.: #66669]	Geographic location (country and/or sea, region)	Udon Thani Province
[Ref.: #66669]	Country	Thailand
[Ref.: #66669]	Country ISO 3 Code	THA
[Ref.: #66669]	Continent	Asia
[Ref.: #66669]	Enrichment culture	humic acid-vitamin medium
[Ref.: #66669]	Enrichment culture composition	supplemented with cycloheximide (50 µg ml-1) and nalidixic acid (25 µg ml-1)
[Ref.: #66669]	Enrichment culture temperature	30 ̊C
[Ref.: #66669]	Isolation/enrichment procedure	The sample was air-dried at room temperature for 7 days before isolation. Then standard dilution-plating technique

[Ref.: #67771]	Sample type/isolated from	From rhizosphere soil of the tree, Azadirachta indica
[Ref.: #67771]	Country	Thailand
[Ref.: #67771]	Country ISO 3 Code	THA
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66669]	Actinomadura rhizosphaerae SDA37 gene for 16S ribosomal RNA, partial sequence	LC369500	1408	ENA	2291546 tax ID

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GC-content

73.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65050]

Culture collection no.

DSM 108648, KCTC 39965, NBRC 112909, TISTR 2523

[Ref.: #111179]

SI-ID 399841

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya.	Hu J, Han C, Yu B, Zhao J, Guo X, Shen Y, Wang X, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003819	2020	*
Phylogeny	Actinomadura rhizosphaerae sp. nov., isolated from rhizosphere soil of the plant Azadirachta indica.	Malisorn K, Kanchanasin P, Phongsopitanun W, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002940	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65050

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108648

#66669

Kingchan Malisorn, Pawina Kanchanasin, Wongsakorn Phongsopitanun, Somboon Tanasupawat: Actinomadura rhizosphaerae sp. nov., isolated from rhizosphere soil of the plant Azadirachta indica. IJSEM 68: 3012 - 3016 2018 ( DOI 10.1099/ijsem.0.002940 , PubMed 30063200 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#111179

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399841.1 )

* These data were automatically processed and therefore are not curated

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