Actinomadura rhizosphaerae (DSM 108648, KCTC 39965, NBRC 112909)

Name: Actinomadura rhizosphaerae (DSM 108648, KCTC 39965, NBRC 112909)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158374

Type strain

Strain Designation SDA37

Culture col. no. DSM 108648 KCTC 39965 NBRC 112909 TISTR 2523 SDA37

NCBI tax ID(s) 2291546

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Actinomadura rhizosphaerae SDA37 is an aerobe, spore-forming, mesophilic prokaryote that was isolated from rhizosphere soil of Azadirachta indica.

spore-forming Gram-positive aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 399841

KCTC 39965,NBRC 112909,DSM 108648

@ref 20215

Last LPSN update 2025-12-16 10:08:00

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura rhizosphaerae

Full scientific name Actinomadura rhizosphaerae Malisorn et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Motility
66669	positive

Colony morphology

@ref	Colony color	Medium used
66669	yellowish brown to greyish yellow	agar medium

Multicellular morphology

@ref	Forms multicellular complex	Complex color	Medium name	Further description
66669		white	ISP3, ISP4	aerial mycelium

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
65050	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
65050	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
65050	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
65050	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
65050	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
65050	BENNETT'S AGAR (DSMZ Medium 548)	Medium recipe at MediaDive	Name: BENNETT'S AGAR (DSMZ Medium 548) Composition: Agar 15.0 g/l Glucose 10.0 g/l N-Z amine 2.0 g/l Yeast extract 1.0 g/l Beef extract 1.0 g/l Distilled water
66669	agar medium

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
65050	positive	growth	28	mesophilic
66669	negative	growth	45
66669	positive	growth	20-40
66669	positive	optimum	30-37	mesophilic
67771	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH
66669	positive	optimum	6-7.5
66669	positive	growth	5-9

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
66669	aerobe
67771	aerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation
66669	The spore is 0.4-0.5 x 0.9-1.2 µm in size and straight or flexuous chains of smooth-ornamented spores.	spore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66669	NaCl	positive	growth	0-4 %(w/v)

Observation

66669

ObservationProduces brown soluble pigment on ISP2, ISP6 and ISP7 media, and yellow soluble pigment on ISP3. No pigment is observed on ISP4 and ISP5.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66669	17057 ChEBI	cellobiose	+	carbon source
66669	62968 ChEBI	cellulose	-	carbon source
66669	15824 ChEBI	D-fructose	+	carbon source
66669	12936 ChEBI	D-galactose	-	carbon source
66669	16899 ChEBI	D-mannitol	-	carbon source
66669	16024 ChEBI	D-mannose	-	carbon source
66669	65327 ChEBI	D-xylose	+	carbon source
66669	5291 ChEBI	gelatin	+	hydrolysis
66669	17754 ChEBI	glycerol	-	carbon source
66669	30849 ChEBI	L-arabinose	+	carbon source
66669	62345 ChEBI	L-rhamnose	-	carbon source
66669	28053 ChEBI	melibiose	-	carbon source
66669	17268 ChEBI	myo-inositol	-	carbon source
66669	17632 ChEBI	nitrate	+	reduction
66669	16634 ChEBI	raffinose	-	carbon source
66669	28017 ChEBI	starch	-	hydrolysis
66669	17992 ChEBI	sucrose	-	carbon source

Enzymes

@ref	Value	Activity	Ec
66669	acid phosphatase	+	3.1.3.2
66669	alkaline phosphatase	+	3.1.3.1
66669	alpha-chymotrypsin	+	3.4.21.1
66669	alpha-fucosidase	+	3.2.1.51
66669	alpha-galactosidase	+	3.2.1.22
66669	alpha-glucosidase	+	3.2.1.20
66669	alpha-mannosidase	+	3.2.1.24
66669	beta-galactosidase	+	3.2.1.23
66669	beta-glucosidase	-	3.2.1.21
66669	beta-glucuronidase	+	3.2.1.31
66669	cystine arylamidase	+	3.4.11.3
66669	esterase (C 4)	+
66669	esterase Lipase (C 8)	+
66669	leucine arylamidase	-	3.4.11.1
66669	lipase (C 14)	+
66669	N-acetyl-beta-glucosaminidase	+	3.2.1.52
66669	naphthol-AS-BI-phosphohydrolase	+
66669	trypsin	+	3.4.21.4
66669	valine arylamidase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
65050	rhizosphere soil of Azadirachta indica	Udon Thani Province	Thailand	THA	Asia
66669	rhizosphere soil of the plant Azadirachta indica	Udon Thani Province	Thailand	THA	Asia
67771	From rhizosphere soil of the tree, Azadirachta indica		Thailand	THA	Asia

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66669	Actinomadura rhizosphaerae SDA37 gene for 16S ribosomal RNA, partial sequence	LC369500	1408		2291546

GC content

@ref	GC-content (mol%)	Method
66669	73.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya.	Hu J, Han C, Yu B, Zhao J, Guo X, Shen Y, Wang X, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003819	2020
Phylogeny	Actinomadura rhizosphaerae sp. nov., isolated from rhizosphere soil of the plant Azadirachta indica.	Malisorn K, Kanchanasin P, Phongsopitanun W, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002940	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65050	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108648
#66669	Kingchan Malisorn, Pawina Kanchanasin, Wongsakorn Phongsopitanun, Somboon Tanasupawat: Actinomadura rhizosphaerae sp. nov., isolated from rhizosphere soil of the plant Azadirachta indica. IJSEM 68: 3012 - 3016 2018 ( DOI 10.1099/ijsem.0.002940 , PubMed 30063200 )
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura rhizosphaerae (DSM 108648, KCTC 39965, NBRC 112909)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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