Name: Actinomadura barringtoniae DSM 108647
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158373

Type strain:

Species: Actinomadura barringtoniae

Culture col. no.: DSM 108647, NBRC 113074, TBRC 7225, GKU 128

Strain history: <- NBRC; NBRC 113074 <- H. Rachniyom, BIOTEC; GKU 128

NCBI tax ID(s): 1427535 (species)

SI-ID 398387

DSM 108647

Section

Actinomadura barringtoniae DSM 108647 is an aerobe, spore-forming, mesophilic bacterium that was isolated from root of Indian oak tree Barringtonia acutangula Gaertn..

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura barringtoniae**
	Full Scientific Name (LPSN)	Actinomadura barringtoniae Rachniyom et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65467]	Gram stain	positive

[Ref.: #65467]	Motility	no

[Ref.: #65467]	Pigment production	no
[Ref.: #65467]	Pigment name	melanin

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium

BHI MEDIUM (DSMZ Medium 215)

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Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

ISP 2 medium

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Culture medium

ISP 4 medium

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Culture medium

ISP 5 medium

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Culture medium

ISP 7 medium

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #65049]			growth	28 °C
[Ref.: #65467]			optimum	24-32 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #65467]			optimum	7

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65467]		NaCl		growth	0-5	%(w/v)

[Ref.: #65467]	Observation	forms extensively branched substrate and aerial mycelia
[Ref.: #65467]	Observation	spores are smooth and oval to cylindrical in shaped (0.6x0.8µm in size)
[Ref.: #65467]	Observation	antique gold pigment is produced on ISP 2 medium
[Ref.: #65467]	Observation	cell-wall peptidoglycan contains meso-diaminopimelic acid and the whole-cell sugars are glucose, madurose, mannose, rhamnose and ribose
[Ref.: #65467]	Observation	N-acyl type of muramic acid is acetyl
[Ref.: #65467]	Observation	mycolic acids are absent
[Ref.: #65467]	Observation	major phospholipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside
[Ref.: #65467]	Observation	predominant menaquinone is MK-9(H6), with minor amounts of MK-9(H8), MK-9(H0) and MK-9(H4)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65467]	(-)-D-fructose ChEBI	-	builds acid from
[Ref.: #65467]	(-)-D-fructose ChEBI	+	carbon source
[Ref.: #65467]	adenine ChEBI	+	assimilation
[Ref.: #65467]	beta-D-xylose ChEBI	-	builds acid from
[Ref.: #65467]	casein	+	assimilation
[Ref.: #65467]	D-glucose ChEBI	-	builds acid from
[Ref.: #65467]	D-glucose ChEBI	+	carbon source
[Ref.: #65467]	D-mannitol ChEBI	+	builds acid from
[Ref.: #65467]	D-mannitol ChEBI	+	carbon source
[Ref.: #65467]	D-mannose ChEBI	-	builds acid from
[Ref.: #65467]	D-mannose ChEBI	+	carbon source
[Ref.: #65467]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65467]	D-sorbitol ChEBI	-	carbon source
[Ref.: #65467]	D-xylose ChEBI	-	carbon source
[Ref.: #65467]	gelatin ChEBI	+	assimilation
[Ref.: #65467]	glycerol ChEBI	-	builds acid from
[Ref.: #65467]	glycerol ChEBI	+	carbon source
[Ref.: #65467]	hypoxanthine ChEBI	+	assimilation
[Ref.: #65467]	L-arabinose ChEBI	+	builds acid from
[Ref.: #65467]	L-arabinose ChEBI	+	carbon source
[Ref.: #65467]	L-arginine ChEBI	+	nitrogen source
[Ref.: #65467]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #65467]	L-cysteine ChEBI	+	nitrogen source
[Ref.: #65467]	L-histidine ChEBI	+	nitrogen source
[Ref.: #65467]	L-proline ChEBI	+	nitrogen source
[Ref.: #65467]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65467]	L-rhamnose ChEBI	+	carbon source
[Ref.: #65467]	L-tyrosine ChEBI	+	assimilation
[Ref.: #65467]	L-valine ChEBI	+	nitrogen source
[Ref.: #65467]	maltose ChEBI	-	builds acid from
[Ref.: #65467]	maltose ChEBI	+	carbon source
[Ref.: #65467]	milk	-	assimilation
[Ref.: #65467]	myo-inositol ChEBI	+	carbon source
[Ref.: #65467]	nitrate ChEBI	+	reduction
[Ref.: #65467]	potassium nitrate ChEBI	+	nitrogen source
[Ref.: #65467]	raffinose ChEBI	-	builds acid from
[Ref.: #65467]	raffinose ChEBI	+	carbon source
[Ref.: #65467]	ribitol ChEBI	+	carbon source
[Ref.: #65467]	sodium citrate ChEBI	-	assimilation
[Ref.: #65467]	starch ChEBI	+	assimilation
[Ref.: #65467]	sucrose ChEBI	+	builds acid from
[Ref.: #65467]	sucrose ChEBI	+	carbon source
[Ref.: #65467]	trehalose ChEBI	+	carbon source
[Ref.: #65467]	tween 20 ChEBI	+	assimilation
[Ref.: #65467]	tween 80 ChEBI	+	assimilation
[Ref.: #65467]	urea ChEBI	-	assimilation
[Ref.: #65467]	xanthine ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #65467]	amphotericin b ChEBI	yes	50	µg/mL
[Ref.: #65467]	ampicillin ChEBI	yes	100	µg/mL
[Ref.: #65467]	cycloheximide ChEBI	yes	50	µg/mL
[Ref.: #65467]	penicillin g ChEBI	yes	2.5	Unit (disc)

	Metabolite production	Metabolite	Production
[Ref.: #65467]		sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65467]	acid phosphatase	+	3.1.3.2
[Ref.: #65467]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65467]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #65467]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65467]	alpha-galactosidase	+	3.2.1.22
[Ref.: #65467]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65467]	alpha-mannosidase	+	3.2.1.24
[Ref.: #65467]	beta-galactosidase	+	3.2.1.23
[Ref.: #65467]	beta-glucosidase	+	3.2.1.21
[Ref.: #65467]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65467]	catalase	+	1.11.1.6
[Ref.: #65467]	cystine arylamidase	+	3.4.11.3
[Ref.: #65467]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65467]	esterase (C 4)	+
[Ref.: #65467]	esterase Lipase (C 8)	+
[Ref.: #65467]	leucine arylamidase	+	3.4.11.1
[Ref.: #65467]	lipase	-
[Ref.: #65467]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #65467]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65467]	trypsin	+	3.4.21.4
[Ref.: #65467]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65049]	Sample type/isolated from	root of Indian oak tree Barringtonia acutangula (L.) Gaertn.
[Ref.: #65049]	Geographic location (country and/or sea, region)	Chantaburi province, Khao Khitchakut district
[Ref.: #65049]	Country	Thailand
[Ref.: #65049]	Country ISO 3 Code	THA
[Ref.: #65049]	Continent	Asia

[Ref.: #65467]	Sample type/isolated from	the root of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.]
[Ref.: #65467]	Geographic location (country and/or sea, region)	Khao Khitchakut district, Chantaburi province
[Ref.: #65467]	Country	Thailand
[Ref.: #65467]	Country ISO 3 Code	THA
[Ref.: #65467]	Continent	Asia
[Ref.: #65467]	Enrichment culture duration	3-4 weeks
[Ref.: #65467]	Enrichment culture temperature	28 ̊C
[Ref.: #65467]	Isolation date	been surface
[Ref.: #65467]	Isolation/enrichment procedure	was isolated from excised roots that had
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Plant	#Root (Rhizome)
#Host	#Plants	#Tree

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65049]	Actinomadura sp. GKU 128 16S ribosomal RNA gene, partial sequence	KF667497	1497	ENA	1427535 tax ID

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GC-content

70.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

DSM 108647, NBRC 113074, TBRC 7225, GKU 128

[Ref.: #111178]

SI-ID 398387

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura violacea sp. nov., a madurastatin A1-producing strain isolated from lichen in Thailand.	Kanchanasin P, Phongsopitanun W, Yuki M, Kudo T, Ohkuma M, Nakashima T, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.005126	2021	*
Phylogeny	Actinomadura barringtoniae sp. nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.	Rachniyom H, Matsumoto A, Inahashi Y, Take A, Takahashi Y, Thamchaipenet A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002714	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65049

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108647

#65467

Hathairat Rachniyom, Atsuko Matsumoto, Yuki Inahashi, Akira Take, Yoko Takahashi, Arinthip Thamchaipenet: Actinomadura barringtoniae sp. nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.. IJSEM 68: 1584 - 1590 2018 ( DOI 10.1099/ijsem.0.002714 , PubMed 29543147 )

#111178

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398387.1 )

* These data were automatically processed and therefore are not curated

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