5 g tryptone peptone; 2.5 g yeast extract; 1 ml resazurin (0.1 % w/v); 40 ml salt solution 1 (per litre: 6 g K2HPO4 ); 40 ml salt solution 2 (per litre: 6 g KH2PO4 ; 12 g (NH4)2SO4 ; 12 g NaCl; 1.2 g MgSO4 . 7H2O; 1.2 g CaCl . 6H2O); 1 ml haemin solution (0.5 g haemin into 10 ml 1M NaOH. Bring up to 100 ml in dH2O. Stored at 4 °C); 1 ml vitamin K working stock (0.02 ml vitamin K into 2 ml 95 % ethanol. Stored in dark at 4 °C); 0.5 g cysteine HCl; 10 g glucose; 300 ml clarified rumen fluid; dH2O
João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.:
MicrobeAtlas 1.0 beta
* These data were automatically processed and therefore are not curated
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