Name: Mycobacterium kyogaense CECT 9646
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158311

Type strain:

Species: Mycobacterium kyogaense

Culture col. no.: CECT 9646, DSM 107316, NCTC 11659

Strain history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; NCTC 11659 <- J.L. Paul and Immodulon Therapeutics Ltd, The school of Pathology, The Middlesex Hospital Medical School; NCTC 11659

NCBI tax ID(s): 2212479 (species)

SI-ID 403136

DSM 107316

Section

Mycobacterium kyogaense CECT 9646 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from mud.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium kyogaense**
	Full Scientific Name (LPSN)	Mycobacterium kyogaense Nouioui et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66749]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #66749]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	no Confidence in %94.094

[Ref.: #66749]	Colony color	yellow-orange
[Ref.: #66749]	Incubation period	5 days
[Ref.: #66749]	Cultivation medium used	Löwenstein-Jensen medium

[Ref.: #66749]	Colony color	yellow-orange
[Ref.: #66749]	Incubation period	5 days
[Ref.: #66749]	Cultivation medium used	Tryptic Soy Agar

[Ref.: #66749]	Colony color	yellow-orange
[Ref.: #66749]	Incubation period	5 days
[Ref.: #66749]	Cultivation medium used	MB7H10 agar

[Ref.: #66749]	Colony color	yellow-orange
[Ref.: #66749]	Incubation period	5 days
[Ref.: #66749]	Cultivation medium used	PMG agar

[Ref.: #66749]	Colony color	yellow-orange
[Ref.: #66749]	Incubation period	5 days
[Ref.: #66749]	Cultivation medium used	glucose-yeast extract-malt extract agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64977]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250)

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Culture medium growth

[Ref.: #64977]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64977]

Culture medium composition

expand / minimize

[Ref.: #66749]

Culture medium

glucose-yeast extract-malt extract agar

[Ref.: #66749]

Culture medium growth

[Ref.: #66749]

Culture medium

Löwenstein-Jensen medium

[Ref.: #66749]

Culture medium growth

[Ref.: #66749]

Culture medium

MB7H10 agar

[Ref.: #66749]

Culture medium growth

[Ref.: #66749]

Culture medium

PMG agar

[Ref.: #66749]

Culture medium growth

[Ref.: #66749]

Culture medium

tryptic soy agar (TSA)

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #64977]		growth	37 °C
[Ref.: #66749]	no	growth	4 °C
[Ref.: #66749]	no	growth	15 °C
[Ref.: #66749]		optimum	37 °C
[Ref.: #66749]	no	growth	42 °C
[Ref.: #66749]	no	growth	45 °C
[Ref.: #66749]		growth	20-28 °C

[Ref.: #64977]	Temperature range	mesophilic
[Ref.: #66749]	Temperature range	psychrophilic
[Ref.: #66749]	Temperature range	mesophilic
[Ref.: #66749]	Temperature range	thermophilic

	Kind of pH		pH
[Ref.: #66749]		optimum	7
[Ref.: #66749]	no	growth	5
[Ref.: #66749]		growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66749]

Oxygen tolerance

aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.87

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66749]		NaCl	no	growth	8	%(w/v)

[Ref.: #66749]

Observation

acid-alcohol fast, rapidly growing species

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66749]	(R)-malic acid ChEBI	+	assimilation
[Ref.: #66749]	(S)-lactate ChEBI	-	assimilation
[Ref.: #66749]	(S)-malic acid ChEBI	+	assimilation
[Ref.: #66749]	2-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #66749]	2-oxobutanoate ChEBI	+	assimilation
[Ref.: #66749]	2-oxoglutarate ChEBI	+	assimilation
[Ref.: #66749]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #66749]	3-O-methyl-D-glucose ChEBI	-	assimilation
[Ref.: #66749]	4-hydroxyphenylacetic acid ChEBI	-	assimilation
[Ref.: #66749]	acetic acid ChEBI	+	assimilation
[Ref.: #66749]	acetoacetate ChEBI	-	assimilation
[Ref.: #66749]	alpha-D-glucose ChEBI	+	assimilation
[Ref.: #66749]	bromosuccinate ChEBI	+	assimilation
[Ref.: #66749]	cellobiose ChEBI	-	assimilation
[Ref.: #66749]	citric acid ChEBI	-	assimilation
[Ref.: #66749]	D-arabitol ChEBI	+	assimilation
[Ref.: #66749]	D-fructose ChEBI	+	assimilation
[Ref.: #66749]	D-galactonic acid lactone ChEBI	-	assimilation
[Ref.: #66749]	D-galactose ChEBI	+	assimilation
[Ref.: #66749]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #66749]	D-glucarate ChEBI	-	assimilation
[Ref.: #66749]	D-gluconate ChEBI	+	assimilation
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	D-glucose 6-phosphate ChEBI	+	assimilation
[Ref.: #66749]	D-glucuronate ChEBI	-	assimilation
[Ref.: #66749]	D-mannitol ChEBI	+	assimilation
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	D-mannose ChEBI	+	assimilation
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	D-serine ChEBI	-	assimilation
[Ref.: #66749]	D-sorbitol ChEBI	-	assimilation
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	dextrin ChEBI	+	assimilation
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #66749]	galactarate ChEBI	-	assimilation
[Ref.: #66749]	gamma-aminobutyric acid ChEBI	+	assimilation
[Ref.: #66749]	gelatin ChEBI	-	assimilation
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #66749]	gentiobiose ChEBI	-	assimilation
[Ref.: #66749]	glucuronamide ChEBI	-	assimilation
[Ref.: #66749]	glycerol ChEBI	+	assimilation
[Ref.: #66749]	glycine-proline ChEBI	+	assimilation
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	inosine ChEBI	-	assimilation
[Ref.: #66749]	L-alanine ChEBI	+	assimilation
[Ref.: #66749]	L-arginine ChEBI	-	assimilation
[Ref.: #66749]	L-aspartate ChEBI	-	assimilation
[Ref.: #66749]	L-fucose ChEBI	-	assimilation
[Ref.: #66749]	L-glutamate ChEBI	+	assimilation
[Ref.: #66749]	L-histidine ChEBI	-	assimilation
[Ref.: #66749]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #66749]	L-rhamnose ChEBI	-	assimilation
[Ref.: #66749]	L-serine ChEBI	+	assimilation
[Ref.: #66749]	lactose ChEBI	-	assimilation
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	maltose ChEBI	-	assimilation
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	melibiose ChEBI	-	assimilation
[Ref.: #66749]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #66749]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #66749]	myo-inositol ChEBI	+	assimilation
[Ref.: #66749]	N-acetyl-D-mannosamine ChEBI	+	assimilation
[Ref.: #66749]	N-acetylgalactosamine ChEBI	-	assimilation
[Ref.: #66749]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66749]	n-acetylneuraminate ChEBI	-	assimilation
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #66749]	potassium tellurite ChEBI	+	assimilation
[Ref.: #66749]	propionate ChEBI	+	assimilation
[Ref.: #66749]	quinate ChEBI	-	assimilation
[Ref.: #66749]	raffinose ChEBI	-	assimilation
[Ref.: #66749]	salicin ChEBI	-	assimilation
[Ref.: #66749]	sucrose ChEBI	+	assimilation
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #66749]	trehalose ChEBI	+	assimilation
[Ref.: #66749]	turanose ChEBI	-	assimilation
[Ref.: #66749]	tween 40 ChEBI	+	assimilation
[Ref.: #66749]	tween 80 ChEBI	+	assimilation
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66749]	acid phosphatase	+	3.1.3.2
[Ref.: #66749]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #66749]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #66749]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #66749]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #66749]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #66749]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #66749]	arylsulfatase	+	3.1.6.1
[Ref.: #66749]	beta-galactosidase	-	3.2.1.23
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #66749]	beta-glucosidase	-	3.2.1.21
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #66749]	beta-glucuronidase	+	3.2.1.31
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #66749]	catalase	+	1.11.1.6
[Ref.: #66749]	cystine arylamidase	+	3.4.11.3
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #66749]	esterase (C 4)	+
[Ref.: #66749]	esterase Lipase (C 8)	+
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #66749]	leucine arylamidase	+	3.4.11.1
[Ref.: #66749]	lipase (C 14)	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #66749]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #66749]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #66749]	trypsin	+	3.4.21.4
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #66749]	urease	+	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #66749]	valine arylamidase	+
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66749]

Fatty acid	Percentage
C16:0	32.3
C18:1 w9c	15
C18:0	9.9
20:0 ALC	9.5
C17:1 w7c/18 alcohol	9.3
C14:0	8.2
C16:1w6c	6.6
C10 Me- 18:0	4.6
C16:1 w9c	2.1

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #64977]	4199	-	+	+/-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #64977]	14077	-	+	+/-	+	-	+/-	-	-	-	-	+/-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64977]	Sample type/isolated from	mud
[Ref.: #64977]	Geographic location (country and/or sea, region)	Lake Kyoga
[Ref.: #64977]	Country	Uganda
[Ref.: #64977]	Country ISO 3 Code	UGA
[Ref.: #64977]	Continent	Africa
[Ref.: #64977]	Geographic location	1.7166°/32.7000°

[Ref.: #66749]	Geographic location (country and/or sea, region)	Kyoga Lake
[Ref.: #66749]	Country	Uganda
[Ref.: #66749]	Country ISO 3 Code	UGA
[Ref.: #66749]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Mud (Sludge)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66749]	Mycobacterium sp. strain NCTC 11659 16S ribosomal RNA gene, partial sequence	MH401762	1529	ENA	2212479 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium kyogaense NCTC 11659	GCA_003254575	contig	GenBank	2212479 tax ID	*
[Ref.: #66792]	Mycobacterium sp. NCTC 11659	2212479.3	wgs	PATRIC	2212479 tax ID	*
[Ref.: #66792]	Mycolicibacterium kyogaense NCTC 11659	2869209271	draft	JGI IMG/M	2212479 tax ID	*

[Ref.: #66749]

GC-content

67.9 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacterium sp. NCTC 11659 PATRIC: 2212479.3
[Ref.: #69481]	spore-forming	no	89	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium sp. NCTC 11659 PATRIC: 2212479.3
[Ref.: #69480]	motile	no	90.911	no
[Ref.: #69480]	gram-positive	yes	88.804	yes
[Ref.: #69480]	anaerobic	no	99.121	yes
[Ref.: #69480]	aerobic	yes	89.315	yes
[Ref.: #69480]	halophile	no	89.216	no
[Ref.: #69480]	spore-forming	no	87.039	no
[Ref.: #69480]	glucose-util	yes	88.887	yes
[Ref.: #69480]	flagellated	no	97.781	no
[Ref.: #69480]	thermophile	no	99.263	no
[Ref.: #69480]	glucose-ferment	no	91.163	no

Availability in culture collections External links

[Ref.: #64977]

Culture collection no.

CECT 9646, DSM 107316, NCTC 11659

[Ref.: #111125]

SI-ID 403136

Literature:

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Rapidly Growing Mycobacterium Species: The Long and Winding Road from Tuberculosis Vaccines to Potent Stress-Resilience Agents.	Amoroso M, Langgartner D, Lowry CA, Reber SO	Int J Mol Sci	10.3390/ijms222312938	2021	*
Phylogeny	Description of a novel species of fast growing mycobacterium: Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae.	Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003039	2018	*
Phylogeny	Mycolicibacterium aurantiacum sp. nov. and Mycolicibacterium xanthum sp. nov., two novel actinobacteria isolated from mangrove sediments.	Pan X, Li Z, Huang S, Huang Y, Wang Q, Tao Z, Hu W	Int J Syst Evol Microbiol	10.1099/ijsem.0.005595	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64977

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107316

#66749

Imen Nouioui, Laura Rosa Brunet, David Simpson, Hans-Peter Klenk, Michael Goodfellow: Description of a novel species of fast growing mycobacterium: Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae. IJSEM 68: 3726 - 3734 2018 ( DOI 10.1099/ijsem.0.003039 , PubMed 30300123 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111125

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403136.1 )

* These data were automatically processed and therefore are not curated

Change proposal

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Search for species Mycobacterium kyogaense in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
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Mycobacterium sp. NCTC 11659

Mycobacterium sp. NCTC 11659

Change proposal

Change proposal