Name: Winogradskyella haliclonae M1A16
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158305

Type strain:

Species: Winogradskyella haliclonae

Strain Designation: M1A16

Culture col. no.: DSM 103138, CCM 8681, CIP 111091, LMG 29588

Strain history: CIP <- 2016, S.Glaeser, Giessen Univ., Giessen, Germany: strain M1A16, Winogradskyella

NCBI tax ID(s): 2048558 (species)

SI-ID 404608

DSM 103138, CIP 111091

Section

Winogradskyella haliclonae M1A16 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from marine sponge of the genus Haliclona.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Winogradskyella*
	Species	**Winogradskyella haliclonae**
	Full Scientific Name (LPSN)	Winogradskyella haliclonae Schellenberg et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65239]	Gram stain	negative
[Ref.: #119792]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #65239]	Cell length	1.5-2.1 µm

[Ref.: #65239]	Cell width	0.6-0.8 µm

[Ref.: #65239]	Cell shape	rod-shaped
[Ref.: #119792]	Cell shape	rod-shaped

[Ref.: #65239]	Motility	yes
[Ref.: #119792]	Motility	no

[Ref.: #65239]	Colony color	yellow
[Ref.: #65239]	Colony shape	circular
[Ref.: #65239]	Incubation period	3 days
[Ref.: #65239]	Cultivation medium used	marine agar 2216

[Ref.: #65239]	Pigment production	no
[Ref.: #65239]	Pigment name	flexirubin-type pigments

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

CIP Medium 13

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

CASO agar

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Culture medium growth

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Culture medium

Columbia agar with sheep blood

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Culture medium growth

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Culture medium

glycine/arginine agar

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Culture medium growth

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Culture medium

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

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Culture medium

Malt agar

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Culture medium growth

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Culture medium

Gym Streptomyces medium

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Culture medium growth

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Culture medium

nutrient agar (NA, Becton Dickinson)

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Culture medium growth

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Culture medium

nutrient agar (Nu, Oxoid)

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Culture medium growth

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Culture medium

nutrient broth

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Culture medium growth

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Culture medium

PYE

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

tryptic soy agar

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #64966]		growth	25 °C
[Ref.: #65239]		optimum	25-28 °C
[Ref.: #65239]		growth	20-32 °C
[Ref.: #65239]	no	growth	<15 °C
[Ref.: #65239]	no	growth	15 °C
[Ref.: #65239]	no	growth	37 °C
[Ref.: #65239]	no	growth	>37 °C

[Ref.: #64966]	Temperature range	mesophilic
[Ref.: #65239]	Temperature range	mesophilic
[Ref.: #65239]	Temperature range	psychrophilic
[Ref.: #65239]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65239]			optimum	7-8
[Ref.: #65239]			growth	5.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65239]

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65239]		NaCl		growth	0-6	%(w/v)
[Ref.: #65239]		NaCl		optimum	3	%(w/v)

[Ref.: #65239]

Observation

Produces yellow, water-soluble pigments with absorption maxima at 431, 450 and 477 nm after extraction in acetone/methanol (7 : 2, v/v).

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65239]	carboxymethylcellulose ChEBI	+	degradation
[Ref.: #65239]	casein	-	hydrolysis
[Ref.: #65239]	dna ChEBI	+	hydrolysis
[Ref.: #65239]	starch ChEBI	+	hydrolysis
[Ref.: #65239]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65239]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65239]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65239]	tween 80 ChEBI	-	hydrolysis

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65239]		catalase	+	1.11.1.6
[Ref.: #65239]		cytochrome oxidase	+	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65239]

Fatty acid	Percentage	ECL
C15:1 iso G	18.1
C15:0 iso	13.7
C16:1ω7c	12.9
C17:0 iso 3OH	10.6
C16:0 iso 3OH	10.2
C15:0 iso 3OH	6.1
C15:0	5.4
C16:0	3.3
C16:0 3OH	3.3
C16:0 iso	3.3
C14:0 iso	3.2
C15:0 anteiso	2.2
Unknown	2.1	14
C15:0 2OH	1.7
C16:1 iso G	1.6
C15:1 anteiso A	1.6
C14:0	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64966]	Sample type/isolated from	marine sponge of the genus Haliclona
[Ref.: #64966]	Geographic location (country and/or sea, region)	Justus-Liebig University Giessen, aquarium facility
[Ref.: #64966]	Country	Germany
[Ref.: #64966]	Country ISO 3 Code	DEU
[Ref.: #64966]	Continent	Europe

[Ref.: #119792]	Sample type/isolated from	Animal, Marine sponge of the genius Haliclona
[Ref.: #119792]	Geographic location (country and/or sea, region)	Giessen, Hessen
[Ref.: #119792]	Country	Germany
[Ref.: #119792]	Country ISO 3 Code	DEU
[Ref.: #119792]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Porifera (Sponges)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX640900 (>99% sequence identity) for Winogradskyella haliclonae from Microbeatlas

Aquatic Samples	149
Soil Samples	4
Animal Samples	9
Plant Samples
Total Samples	162

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64966]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119792]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64966]	Winogradskyella haliclonae strain M1A16 16S ribosomal RNA gene, partial sequence	KX640900	1430	ENA	2048558 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Winogradskyella haliclonae CCM 8681	GCA_014635865	contig	GenBank	2048558 tax ID	*
[Ref.: #66792]	Winogradskyella haliclonae strain CCM 8681	2048558.3	wgs	PATRIC	2048558 tax ID	*

[Ref.: #64966]	GC-content	32.7 mol%	fluorimetric
[Ref.: #65239]	GC-content	32.7 mol%	Thermal denaturation, fluorometry

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Winogradskyella haliclonae strain CCM 8681 PATRIC: 2048558.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Winogradskyella haliclonae strain CCM 8681 PATRIC: 2048558.3
[Ref.: #69480]	motile	no	86.72	yes
[Ref.: #69480]	gram-positive	no	97.951	yes
[Ref.: #69480]	anaerobic	no	99.405	yes
[Ref.: #69480]	aerobic	yes	87.869	no
[Ref.: #69480]	halophile	no	71.783	no
[Ref.: #69480]	spore-forming	no	95.487	no
[Ref.: #69480]	glucose-util	yes	83.958	no
[Ref.: #69480]	flagellated	no	95.833	no
[Ref.: #69480]	thermophile	no	98.952	no
[Ref.: #69480]	glucose-ferment	no	91.397	no

Availability in culture collections External links

[Ref.: #64966]

Culture collection no.

DSM 103138, CCM 8681, CIP 111091, LMG 29588

[Ref.: #111119]

SI-ID 404608

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Winogradskyella haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona.	Schellenberg J, Busse HJ, Hardt M, Schubert P, Wilke T, Kampfer P, Glaeser SP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002192	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64966

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103138

#65239

Johannes Schellenberg, Hans-Jürgen Busse, Martin Hardt, Patrick Schubert, Thomas Wilke, Peter Kämpfer, Stefanie P. Glaeser: Winogradskyella haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona. IJSEM 67: 4902 - 4910 2017 ( DOI 10.1099/ijsem.0.002192 , PubMed 29058658 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111119

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404608.1 )

#119792 Collection of Institut Pasteur ; Curators of the CIP; CIP 111091
* These data were automatically processed and therefore are not curated

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Winogradskyella haliclonae strain CCM 8681

Winogradskyella haliclonae strain CCM 8681

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