Strain identifier

BacDive ID: 158306

Type strain: Yes

Species: Monoglobus pectinilyticus

Strain Designation: 14

Culture col. no.: DSM 104782, JCM 31914

Strain history: D. Rosendale; Plant & Food Res., New Zealand; 14.

NCBI tax ID(s): 1981510 (species)

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Monoglobus pectinilyticus 14 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from fresh human faecal sample of a healthy 27-year-old woman.

  1. coccus-shaped
  2. Gram-positive
  3. anaerobe
  4. mesophilic
  5. 16S sequence
  6. Bacteria
  7. genome sequence
  • Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental informationarrow_down
  • [Ref.: #64968] Sample type/isolated from fresh human faecal sample of a healthy 27-year-old woman
    [Ref.: #64968] Geographic location (country and/or sea, region) Palmerston North (40.3523° S, 175.6082° E)
    [Ref.: #64968] Country New Zealand
    [Ref.: #64968] Country ISO 3 Code NZL
    [Ref.: #64968] Continent Australia and Oceania
    [Ref.: #64968] Geographic location -40.3523°/175.6080°
     
    [Ref.: #65227] Sample type/isolated from faeces from a healthy 27-year-old female living in New Zealand who had not been prescribed antibiotics for 6 months prior to the sample collection
    [Ref.: #65227] Geographic location (country and/or sea, region) The New Zealand Institute for Plant and Food Research Limited, Palmerston North
    [Ref.: #65227] Country New Zealand
    [Ref.: #65227] Country ISO 3 Code NZL
    [Ref.: #65227] Continent Australia and Oceania
    [Ref.: #65227] Enrichment culture basal medium (10 mL)
    [Ref.: #65227] Enrichment culture composition (per litre) 2 g Difco peptone water; 2 g Bacto yeast extract; 0.5 g bile salts (Oxoid); 10 ml Mineral A stock solution (0.4 g K2HPO4 dissolved in 100 ml distilled water); 10 ml Mineral B stock solution (0.4 g KH2PO4 ; 0.1 g MgSO4x7H2O; 0.1 g CaCl2x6H2O; and 1.0 g NaCl dissolved in 100 ml distilled water); 2 ml Tween 80 (Fischer Scientific); 0.05 g haemin (Sigma); 10 µl vitamin K (Sigma); 0.5 g L-cysteine-HCl; 0.02 g resazurin; and 4.2 g NaHCO3 . The basal medium was prepared under O2 -free 100 %
    [Ref.: #65227] Enrichment culture duration 15 days
    [Ref.: #65227] Enrichment culture temperature 37  ̊C
    [Ref.: #65227] Isolation/enrichment procedure Fresh stool was weighed and 5 g of stool was mixed with anaerobically prepared phosphate buffered saline (PBS) solution to make a 10 % faecal slurry. The faecal slurry was serially diluted (10-fold) until 10^9-fold dilution was achieved, and 0.5 ml of 10^-7, 10^-8, and 10^-9 dilutions were used to inoculate the basal medium (10 ml per tube) containing 0.2 % (w/v) kiwifruit pectin. After 15 days of incubation at 37°C with constant rotary shaking, a small volume of culture was spread over the surf
     
    [Ref.: #67770] Sample type/isolated from Healthy 27-yearold female
    [Ref.: #67770] Country New Zealand
    [Ref.: #67770] Country ISO 3 Code NZL
    [Ref.: #67770] Continent Australia and Oceania
    * marker position based on {}
    Isolation sources categories
    #Host Body Product #Gastrointestinal tract #Feces (Stool)
    #Host #Human #Female

    Global distribution of 16S sequence KY978733 (>99% sequence identity) for Monoglobus pectinilyticus subclade from Microbeatlas

    Aquatic Samples 1942
    Soil Samples 218
    Animal Samples 58083
    Plant Samples 141
    Total Samples 60384

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