[Ref.: #64968] |
Sample type/isolated from |
fresh human faecal sample of a healthy 27-year-old woman |
[Ref.: #64968] |
Geographic location (country and/or sea, region) |
Palmerston North (40.3523° S, 175.6082° E) |
[Ref.: #64968] |
Country |
New Zealand |
[Ref.: #64968] |
Country ISO 3 Code |
NZL |
[Ref.: #64968] |
Continent |
Australia and Oceania |
[Ref.: #64968] |
Geographic location |
-40.3523°/175.6080° |
|
[Ref.: #65227] |
Sample type/isolated from |
faeces from a healthy 27-year-old female living in New Zealand who had not been prescribed antibiotics for 6 months prior to the sample collection |
[Ref.: #65227] |
Geographic location (country and/or sea, region) |
The New Zealand Institute for Plant and Food Research Limited, Palmerston North |
[Ref.: #65227] |
Country |
New Zealand |
[Ref.: #65227] |
Country ISO 3 Code |
NZL |
[Ref.: #65227] |
Continent |
Australia and Oceania |
[Ref.: #65227] |
Enrichment culture |
basal medium (10 mL) |
[Ref.: #65227] |
Enrichment culture composition |
(per litre) 2 g Difco peptone water; 2 g Bacto yeast extract; 0.5 g bile salts (Oxoid); 10 ml Mineral A stock solution (0.4 g K2HPO4 dissolved in 100 ml distilled water); 10 ml Mineral B stock solution (0.4 g KH2PO4 ; 0.1 g MgSO4x7H2O; 0.1 g CaCl2x6H2O; and 1.0 g NaCl dissolved in 100 ml distilled water); 2 ml Tween 80 (Fischer Scientific); 0.05 g haemin (Sigma); 10 µl vitamin K (Sigma); 0.5 g L-cysteine-HCl; 0.02 g resazurin; and 4.2 g NaHCO3 . The basal medium was prepared under O2 -free 100 % |
[Ref.: #65227] |
Enrichment culture duration |
15 days |
[Ref.: #65227] |
Enrichment culture temperature |
37 ̊C |
[Ref.: #65227] |
Isolation/enrichment procedure |
Fresh stool was weighed and 5 g of stool was mixed with anaerobically prepared phosphate buffered saline (PBS) solution to make a 10 % faecal slurry. The faecal slurry was serially diluted (10-fold) until 10^9-fold dilution was achieved, and 0.5 ml of 10^-7, 10^-8, and 10^-9 dilutions were used to inoculate the basal medium (10 ml per tube) containing 0.2 % (w/v) kiwifruit pectin. After 15 days of incubation at 37°C with constant rotary shaking, a small volume of culture was spread over the surf |
|
[Ref.: #67770] |
Sample type/isolated from |
Healthy 27-yearold female |
[Ref.: #67770] |
Country |
New Zealand |
[Ref.: #67770] |
Country ISO 3 Code |
NZL |
[Ref.: #67770] |
Continent |
Australia and Oceania |
* marker position based on {}
|
|
Isolation sources categories |
#Host Body Product |
#Gastrointestinal tract |
#Feces (Stool) |
#Host |
#Human |
#Female |
|
|
-
Availability in culture collections External links
[Ref.: #64968] |
Culture collection no. |
DSM 104782, JCM 31914 |
[Ref.: #111120] |
SI-ID 398739
|
* |
|
Literature: |
|
Topic |
Title |
Authors |
Journal |
DOI |
Year |
|
Phylogeny |
Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces. |
Kim CC, Kelly WJ, Patchett ML, Tannock GW, Jordens Z, Stoklosinski HM, Taylor JW, Sims IM, Bell TJ, Rosendale DI |
Int J Syst Evol Microbiol |
10.1099/ijsem.0.002395 |
2017 |
* |
|
- References
-
-
#64968 |
Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ;
DSM 104782
|
-
#65227 |
Caroline C. Kim, William J. Kelly, Mark L. Patchett, Gerald W. Tannock, Z. Jordens, Halina M. Stoklosinski, Jordan W. Taylor, Ian M. Sims, Tracey J. Bell, Douglas I. Rosendale:
Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces.
IJSEM 67:
4992 - 4998
2017 (
DOI 10.1099/ijsem.0.002395 , PubMed 29039307 )
|
-
-
#66794 |
Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg:
BRENDA, the ELIXIR core data resource in 2021: new developments and updates.
Nucleic Acids Res. 49:
D498 - D508
2020 (
DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
|
-
#67770 |
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
|
-
#69479 |
João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.:
MicrobeAtlas 1.0 beta
.
|
-
-
-
#111120 |
Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.:
StrainInfo: A central database for resolving microbial strain identifiers
.
(
DOI 10.60712/SI-ID398739.1 )
|
- * These data were automatically processed and therefore are not curated
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