Name: Psychrobacillus lasiicapitis NEAU-3TGS17
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158270

Type strain:

Species: Psychrobacillus lasiicapitis

Strain Designation: NEAU-3TGS17

Culture col. no.: DSM 100484, CGMCC 1.15308

Strain history: <- C. Liu, Northeast Agricultural Univ., Harbin, China; NEAU-3TGS17

NCBI tax ID(s): 1636719 (species)

SI-ID 403875

DSM 100484

Special Collections

Section

Psychrobacillus lasiicapitis NEAU-3TGS17 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from head of an ant .

Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Psychrobacillus*
	Species	**Psychrobacillus lasiicapitis**
	Full Scientific Name (LPSN)	Psychrobacillus lasiicapitis Shen et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65284]	Gram stain	positive

[Ref.: #65284]	Cell length	1.9-2.1 µm

[Ref.: #65284]	Cell width	0.6-0.7 µm

[Ref.: #65284]	Cell shape	rod-shaped

[Ref.: #65284]	Motility	yes

[Ref.: #65284]	Flagellum arrangement	peritrichous

Information on culture and growth conditions Culture and growth conditions

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Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

tryptic soy agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #64929]	growth	28 °C
[Ref.: #65284]	growth	0-35 °C
[Ref.: #65284]	optimum	28-30 °C

	pH	Kind of pH		pH
[Ref.: #65284]			optimum	7-8
[Ref.: #65284]		no	growth	6-11

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #65284]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %90.422
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65284]		NaCl		growth	0-6	%(w/v)
[Ref.: #65284]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65284]	amygdalin ChEBI	-	builds acid from
[Ref.: #65284]	arbutin ChEBI	-	builds acid from
[Ref.: #65284]	cellobiose ChEBI	-	builds acid from
[Ref.: #65284]	cellulose ChEBI	-	assimilation
[Ref.: #65284]	citrate ChEBI	-	assimilation
[Ref.: #65284]	creatine ChEBI	+	carbon source
[Ref.: #65284]	creatine ChEBI	+	nitrogen source
[Ref.: #65284]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65284]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65284]	D-fructose ChEBI	-	builds acid from
[Ref.: #65284]	D-fucose ChEBI	-	builds acid from
[Ref.: #65284]	D-galactose ChEBI	-	builds acid from
[Ref.: #65284]	D-glucose ChEBI	-	builds acid from
[Ref.: #65284]	D-glucose ChEBI	+	carbon source
[Ref.: #65284]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65284]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65284]	D-mannitol ChEBI	-	carbon source
[Ref.: #65284]	D-mannose ChEBI	-	builds acid from
[Ref.: #65284]	D-ribose ChEBI	-	builds acid from
[Ref.: #65284]	D-ribose ChEBI	-	carbon source
[Ref.: #65284]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65284]	D-sorbitol ChEBI	-	carbon source
[Ref.: #65284]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65284]	D-xylose ChEBI	-	builds acid from
[Ref.: #65284]	D-xylose ChEBI	+	carbon source
[Ref.: #65284]	erythritol ChEBI	-	builds acid from
[Ref.: #65284]	esculin ChEBI	-	hydrolysis
[Ref.: #65284]	esculin ferric citrate	-	builds acid from
[Ref.: #65284]	galactitol ChEBI	-	builds acid from
[Ref.: #65284]	gelatin ChEBI	-	hydrolysis
[Ref.: #65284]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65284]	glucose ChEBI	-	fermentation
[Ref.: #65284]	glycerol ChEBI	-	builds acid from
[Ref.: #65284]	glycogen ChEBI	-	builds acid from
[Ref.: #65284]	inulin ChEBI	-	builds acid from
[Ref.: #65284]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65284]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65284]	L-arginine ChEBI	+	carbon source
[Ref.: #65284]	L-arginine ChEBI	+	nitrogen source
[Ref.: #65284]	L-aspartate ChEBI	-	carbon source
[Ref.: #65284]	L-aspartate ChEBI	-	nitrogen source
[Ref.: #65284]	L-fucose ChEBI	-	builds acid from
[Ref.: #65284]	L-glutamate ChEBI	-	carbon source
[Ref.: #65284]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #65284]	L-proline ChEBI	+	carbon source
[Ref.: #65284]	L-proline ChEBI	+	nitrogen source
[Ref.: #65284]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65284]	L-serine ChEBI	-	carbon source
[Ref.: #65284]	L-serine ChEBI	-	nitrogen source
[Ref.: #65284]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65284]	L-threonine ChEBI	-	carbon source
[Ref.: #65284]	L-threonine ChEBI	-	nitrogen source
[Ref.: #65284]	L-tyrosine ChEBI	-	carbon source
[Ref.: #65284]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #65284]	L-xylose ChEBI	-	builds acid from
[Ref.: #65284]	lactose ChEBI	-	builds acid from
[Ref.: #65284]	lactose ChEBI	-	carbon source
[Ref.: #65284]	maltose ChEBI	-	builds acid from
[Ref.: #65284]	maltose ChEBI	+	carbon source
[Ref.: #65284]	melezitose ChEBI	-	builds acid from
[Ref.: #65284]	melibiose ChEBI	-	builds acid from
[Ref.: #65284]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65284]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65284]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65284]	milk	-	assimilation
[Ref.: #65284]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65284]	myo-inositol ChEBI	+	carbon source
[Ref.: #65284]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65284]	nitrate ChEBI	+	reduction
[Ref.: #65284]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65284]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65284]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65284]	raffinose ChEBI	-	builds acid from
[Ref.: #65284]	raffinose ChEBI	+	carbon source
[Ref.: #65284]	ribitol ChEBI	-	builds acid from
[Ref.: #65284]	salicin ChEBI	-	builds acid from
[Ref.: #65284]	starch ChEBI	-	builds acid from
[Ref.: #65284]	starch ChEBI	-	hydrolysis
[Ref.: #65284]	sucrose ChEBI	-	builds acid from
[Ref.: #65284]	sucrose ChEBI	+	carbon source
[Ref.: #65284]	trehalose ChEBI	-	builds acid from
[Ref.: #65284]	turanose ChEBI	-	builds acid from
[Ref.: #65284]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65284]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65284]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65284]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65284]	ampicillin ChEBI	yes	200	µg/mL
[Ref.: #65284]	apramycin ChEBI	yes	30	µg/mL
[Ref.: #65284]	chloramphenicol ChEBI	yes	25	µg/mL
[Ref.: #65284]	cycloheximide ChEBI				yes	50	µg/mL
[Ref.: #65284]	erythromycin ChEBI	yes	100	µg/mL
[Ref.: #65284]	hygromycin ChEBI	yes	50	µg/mL
[Ref.: #65284]	kanamycin ChEBI	yes	25	µg/mL
[Ref.: #65284]	nalidixic acid ChEBI				yes	50	µg/mL
[Ref.: #65284]	neomycin ChEBI	yes	30	µg/mL
[Ref.: #65284]	thiostrepton ChEBI	yes	25	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #65284]		acetoin ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #65284]		acetoin ChEBI	-
[Ref.: #65284]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #65284]	acid phosphatase	+	3.1.3.2
[Ref.: #65284]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65284]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65284]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65284]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65284]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65284]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65284]	beta-galactosidase	-	3.2.1.23
[Ref.: #65284]	beta-glucosidase	-	3.2.1.21
[Ref.: #65284]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65284]	cystine arylamidase	-	3.4.11.3
[Ref.: #65284]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65284]	esterase (C 4)	+
[Ref.: #65284]	esterase Lipase (C 8)	+
[Ref.: #65284]	lipase (C 14)	-
[Ref.: #65284]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65284]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65284]	trypsin	-	3.4.21.4
[Ref.: #65284]	urease	+	3.5.1.5
[Ref.: #65284]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64929]	Sample type/isolated from	head of an ant (Lasius fuliginosus)
[Ref.: #64929]	Geographic location (country and/or sea, region)	Heilongjiang Province, Harbin, campus of Northeast Agriculture University
[Ref.: #64929]	Country	China
[Ref.: #64929]	Country ISO 3 Code	CHN
[Ref.: #64929]	Continent	Asia

[Ref.: #65284]	Sample type/isolated from	isolated from the head of L. fuliginosus
[Ref.: #65284]	Geographic location (country and/or sea, region)	Northeast Agriculture University, Harbin, Heilongjiang
[Ref.: #65284]	Country	China
[Ref.: #65284]	Country ISO 3 Code	CHN
[Ref.: #65284]	Continent	Asia
[Ref.: #65284]	Enrichment culture	nutrient agar
[Ref.: #65284]	Enrichment culture duration	10 days
[Ref.: #65284]	Enrichment culture temperature	28 ̊C
[Ref.: #65284]	Isolation/enrichment procedure	Surface-disinfected individual ants were divided into head, mesosoma and gaster, and each body part was separately placed into 500 µl sterile water with shaking on a rotary shaker at 180 r.p.m. at 28°C for 30 min. Subsequently, a 200 µl sample of the suspension of the heads was spread on nutrient agar. After 10 days of aerobic incubation at 28°C.
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Other	#Head
#Host	#Arthropoda	#Insecta

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP219721 (>99% sequence identity) for Psychrobacillus from Microbeatlas

Aquatic Samples	3
Soil Samples	15
Animal Samples	3
Plant Samples	1
Total Samples	22

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64929]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64929]	Psychrobacillus lasiicapitis strain NEAU-3TGS17 16S ribosomal RNA gene, partial sequence	KP219721	1518	GenBank ENA	1636719 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Psychrobacillus lasiicapitis NEAU-3TGS17	GCA_006861795	scaffold	GenBank ENA	1636719 tax ID	*
[Ref.: #66792]	Psychrobacillus lasiicapitis CGMCC 1.15308	GCA_014641675	scaffold	GenBank ENA	1636719 tax ID	*
[Ref.: #66792]	Psychrobacillus lasiicapitis strain CGMCC 1.15308	1636719.5	wgs	PATRIC	1636719 tax ID	*
[Ref.: #66792]	Psychrobacillus lasiicapitis strain NEAU-3TGS17	1636719.3	wgs	PATRIC	1636719 tax ID	*
[Ref.: #66792]	Psychrobacillus lasiicapitis NEAU-3TGS17	2890957088	draft	JGI IMG/M	1636719 tax ID	*

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GC-content

35.8 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Psychrobacillus lasiicapitis NEAU-3TGS17 NCBI: GCA_006861795.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Psychrobacillus lasiicapitis NEAU-3TGS17 NCBI: GCA_006861795.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	73.814	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	95.364	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	82.027	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	90.422	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.892	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	83.91	yes

Availability in culture collections External links

[Ref.: #64929]

Culture collection no.

DSM 100484, CGMCC 1.15308

[Ref.: #111088]

SI-ID 403875

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus).	Shen Y, Fu Y, Yu Y, Zhao J, Li J, Li Y, Wang X, Zhang J, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.002315	2017	*
Phylogeny	Psychrobacillus vulpis sp. nov., a new species isolated from faeces of a red fox in Spain.	Rodriguez M, Reina JC, Bejar V, Llamas I	Int J Syst Evol Microbiol	10.1099/ijsem.0.003840	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64929

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100484

#65284

Yibo Shen, Yansong Fu, Yang Yu, Junwei Zhao, Jiansong Li, Yao Li, Xiangjing Wang, Ji Zhang, Wensheng Xiang: Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus). IJSEM 67: 4462 - 4467 2017 ( DOI 10.1099/ijsem.0.002315 , PubMed 28933325 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111088

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403875.1 )

* These data were automatically processed and therefore are not curated

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