Strain identifier

BacDive ID: 158270

Type strain: Yes

Species: Psychrobacillus lasiicapitis

Strain Designation: NEAU-3TGS17

Strain history: <- C. Liu, Northeast Agricultural Univ., Harbin, China; NEAU-3TGS17

NCBI tax ID(s): 1636719 (species)

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General

@ref: 64929

BacDive-ID: 158270

DSM-Number: 100484

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Psychrobacillus lasiicapitis NEAU-3TGS17 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from head of an ant .

NCBI tax id

  • NCBI tax id: 1636719
  • Matching level: species

strain history

  • @ref: 64929
  • history: <- C. Liu, Northeast Agricultural Univ., Harbin, China; NEAU-3TGS17

doi: 10.13145/bacdive158270.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Psychrobacillus
  • species: Psychrobacillus lasiicapitis
  • full scientific name: Psychrobacillus lasiicapitis Shen et al. 2017

@ref: 64929

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Caryophanaceae

genus: Psychrobacillus

species: Psychrobacillus lasiicapitis

full scientific name: Psychrobacillus lasiicapitis Shen et al. 2017

strain designation: NEAU-3TGS17

type strain: yes

Morphology

cell morphology

  • @ref: 65284
  • gram stain: positive
  • cell length: 1.9-2.1 µm
  • cell width: 0.6-0.7 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64929CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
65284tryptic soy agaryes
65284Nutrient agar (NA)yes

culture temp

@refgrowthtypetemperature
64929positivegrowth28
65284positivegrowth0-35
65284positiveoptimum28-30

culture pH

@refabilitytypepH
65284nogrowth6-11
65284positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 65284
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
65284yes
69480yes90.422
69481yes100

halophily

@refsaltgrowthtested relationconcentration
65284NaClpositivegrowth0-6 %(w/v)
65284NaClpositiveoptimum0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6528427613amygdalin-builds acid from
6528418305arbutin-builds acid from
6528417057cellobiose-builds acid from
6528462968cellulose-assimilation
6528416947citrate-assimilation
6528416919creatine+carbon source
6528416919creatine+nitrogen source
6528415963ribitol-builds acid from
6528417108D-arabinose-builds acid from
6528418333D-arabitol-builds acid from
6528415824D-fructose-builds acid from
6528428847D-fucose-builds acid from
6528412936D-galactose-builds acid from
6528417634D-glucose-builds acid from
6528417634D-glucose+carbon source
6528462318D-lyxose-builds acid from
6528416899D-mannitol-builds acid from
6528416899D-mannitol-carbon source
6528416024D-mannose-builds acid from
6528416988D-ribose-builds acid from
6528416988D-ribose-carbon source
6528417924D-sorbitol-builds acid from
6528417924D-sorbitol-carbon source
6528416443D-tagatose-builds acid from
6528465327D-xylose-builds acid from
6528465327D-xylose+carbon source
6528416813galactitol-builds acid from
6528417113erythritol-builds acid from
652844853esculin-hydrolysis
65284esculin ferric citrate-builds acid from
652845291gelatin-hydrolysis
6528428066gentiobiose-builds acid from
6528417234glucose-fermentation
6528417754glycerol-builds acid from
6528428087glycogen-builds acid from
6528417268myo-inositol-builds acid from
6528417268myo-inositol+carbon source
6528415443inulin-builds acid from
6528430849L-arabinose-builds acid from
6528418403L-arabitol-builds acid from
6528416467L-arginine+carbon source
6528416467L-arginine+nitrogen source
6528429991L-aspartate-carbon source
6528429991L-aspartate-nitrogen source
6528418287L-fucose-builds acid from
6528429985L-glutamate-carbon source
6528429985L-glutamate-nitrogen source
6528417203L-proline+carbon source
6528417203L-proline+nitrogen source
6528462345L-rhamnose-builds acid from
6528417115L-serine-carbon source
6528417115L-serine-nitrogen source
6528417266L-sorbose-builds acid from
6528416857L-threonine-carbon source
6528416857L-threonine-nitrogen source
6528417895L-tyrosine-carbon source
6528417895L-tyrosine-nitrogen source
6528465328L-xylose-builds acid from
6528417716lactose-builds acid from
6528417716lactose-carbon source
6528417306maltose-builds acid from
6528417306maltose+carbon source
652846731melezitose-builds acid from
6528428053melibiose-builds acid from
65284320061methyl alpha-D-glucopyranoside-builds acid from
6528443943methyl alpha-D-mannoside-builds acid from
6528474863methyl beta-D-xylopyranoside-builds acid from
65284milk-assimilation
65284506227N-acetylglucosamine-builds acid from
6528417632nitrate+reduction
65284potassium 2-dehydro-D-gluconate-builds acid from
65284potassium 5-dehydro-D-gluconate-builds acid from
6528432032potassium gluconate-builds acid from
6528416634raffinose-builds acid from
6528416634raffinose+carbon source
6528417814salicin-builds acid from
6528428017starch-builds acid from
6528428017starch-hydrolysis
6528417992sucrose-builds acid from
6528417992sucrose+carbon source
6528427082trehalose-builds acid from
6528432528turanose-builds acid from
6528453424tween 20+hydrolysis
6528453423tween 40+hydrolysis
6528453426tween 80+hydrolysis
6528417151xylitol-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
652846104kanamycinyesyes25 µg/mL
6528417698chloramphenicolyesyes25 µg/mL
6528429693thiostreptonyesyes25 µg/mL
652847507neomycinyesyes30 µg/mL
652842790apramycinyesyes30 µg/mL
6528424753hygromycinyesyes50 µg/mL
6528448923erythromycinyesyes100 µg/mL
6528428971ampicillinyesyes200 µg/mL
65284100147nalidixic acidyesyes50 µg/mL
6528427641cycloheximideyesyes50 µg/mL

metabolite production

  • @ref: 65284
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
6528417234glucose-
6528415688acetoin-

enzymes

@refvalueactivityec
65284urease+3.5.1.5
65284cytochrome oxidase+1.9.3.1
65284alkaline phosphatase+3.1.3.1
65284esterase (C 4)+
65284esterase Lipase (C 8)+
65284naphthol-AS-BI-phosphohydrolase+
65284acid phosphatase+3.1.3.2
65284valine arylamidase+
65284alpha-chymotrypsin-3.4.21.1
65284cystine arylamidase-3.4.11.3
65284alpha-fucosidase-3.2.1.51
65284alpha-galactosidase-3.2.1.22
65284beta-galactosidase-3.2.1.23
65284beta-glucuronidase-3.2.1.31
65284alpha-glucosidase-3.2.1.20
65284beta-glucosidase-3.2.1.21
65284N-acetyl-beta-glucosaminidase-3.2.1.52
65284lipase (C 14)-
65284alpha-mannosidase-3.2.1.24
65284trypsin-3.4.21.4

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
64929head of an ant (Lasius fuliginosus)Heilongjiang Province, Harbin, campus of Northeast Agriculture UniversityChinaCHNAsia
65284isolated from the head of L. fuliginosusNortheast Agriculture University, Harbin, HeilongjiangChinaCHNAsianutrient agar10 days28Surface-disinfected individual ants were divided into head, mesosoma and gaster, and each body part was separately placed into 500 µl sterile water with shaking on a rotary shaker at 180 r.p.m. at 28°C for 30 min. Subsequently, a 200 µl sample of the suspension of the heads was spread on nutrient agar. After 10 days of aerobic incubation at 28°C.

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Head
#Host#Arthropoda#Insecta

taxonmaps

  • @ref: 69479
  • File name: preview.99_18583.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_221;97_240;98_269;99_18583&stattab=map
  • Last taxonomy: Psychrobacillus
  • 16S sequence: KP219721
  • Sequence Identity:
  • Total samples: 22
  • soil counts: 15
  • aquatic counts: 3
  • animal counts: 3
  • plant counts: 1

Safety information

risk assessment

  • @ref: 64929
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64929
  • description: Psychrobacillus lasiicapitis strain NEAU-3TGS17 16S ribosomal RNA gene, partial sequence
  • accession: KP219721
  • length: 1518
  • database: nuccore
  • NCBI tax ID: 1636719

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Psychrobacillus lasiicapitis NEAU-3TGS17GCA_006861795scaffoldncbi1636719
66792Psychrobacillus lasiicapitis CGMCC 1.15308GCA_014641675scaffoldncbi1636719
66792Psychrobacillus lasiicapitis strain CGMCC 1.153081636719.5wgspatric1636719
66792Psychrobacillus lasiicapitis strain NEAU-3TGS171636719.3wgspatric1636719
66792Psychrobacillus lasiicapitis NEAU-3TGS172890957088draftimg1636719

GC content

  • @ref: 64929
  • GC-content: 35.8
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes73.814no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.364no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes82.027yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes90.422yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno93.892yes
69480flagellatedmotile2+Ability to perform flagellated movementyes83.91yes

External links

@ref: 64929

culture collection no.: DSM 100484, CGMCC 1.15308

straininfo link

  • @ref: 111088
  • straininfo: 403875

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28933325Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus).Shen Y, Fu Y, Yu Y, Zhao J, Li J, Li Y, Wang X, Zhang J, Xiang WInt J Syst Evol Microbiol10.1099/ijsem.0.0023152017Animals, Ants/*microbiology, Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny31833832Psychrobacillus vulpis sp. nov., a new species isolated from faeces of a red fox in Spain.Rodriguez M, Reina JC, Bejar V, Llamas IInt J Syst Evol Microbiol10.1099/ijsem.0.0038402020Animals, Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Foxes/*microbiology, Genes, Bacterial, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64929Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100484Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100484)
65284Yibo Shen, Yansong Fu, Yang Yu, Junwei Zhao, Jiansong Li, Yao Li, Xiangjing Wang, Ji Zhang, Wensheng XiangPsychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus)10.1099/ijsem.0.002315IJSEM 67: 4462-4467 201728933325
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111088Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403875.1