Name: Nitrosopumilus adriaticus NF5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 157948

Type strain:

Species: Nitrosopumilus adriaticus

Strain Designation: NF5

Culture col. no.: DSM 106890, JCM 32270, NCIMB 15114

Strain history: G. J. Herndl; Univ. of Vienna, Austria; NF5.

NCBI tax ID(s): 1580092 (species)

Section

Nitrosopumilus adriaticus NF5 is an aerobe, mesophilic, rod-shaped archaeon that was isolated from coastal surface water.

rod-shaped
aerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Nitrososphaerota*
	Class	*Nitrososphaeria*
	Order	*Nitrosopumilales*
	Family	*Nitrosopumilaceae*
	Genus	*Nitrosopumilus*
	Species	**Nitrosopumilus adriaticus**
	Full Scientific Name (LPSN)	Nitrosopumilus adriaticus Bayer et al. 2019

Synonym

"Candidatus Nitrosopumilus adriaticus"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.858

[Ref.: #67883]	Cell length	0.59-1.74 µm

[Ref.: #67883]	Cell width	0.2-0.25 µm

[Ref.: #67883]	Cell shape	rod-shaped

Multimedia content

[Ref.: #64520]

Caption:

Medium 1630a, 30°C, cell suspension concentrated by centrifugation

[Ref.: #64520]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	30 °C
[Ref.: #67883]	growth	15-34 °C
[Ref.: #67883]	optimum	30-32 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67883]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #67883]			optimum	7.1
[Ref.: #67883]			growth	6.8-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67883]

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.994
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67883]		NaCl		growth	10-55	%
[Ref.: #67883]		NaCl		optimum	34	%

[Ref.: #67770]	Observation	quinones: MK-6
[Ref.: #67883]	Observation	Cells are occasionally flagellated.
[Ref.: #67883]	Observation	The minimum generation time is approximately 34 h.

[Ref.: #67883]	Name of tolerated compound	Ammonia
[Ref.: #67883]	Tolerated concentration	25
[Ref.: #67883]	Name of tolerated compound	Nitrite
[Ref.: #67883]	Tolerated concentration	10

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #67883]	carbenicillin ChEBI				yes	100	µg/mL
[Ref.: #67883]	chloramphenicol ChEBI	yes	25	µg/mL
[Ref.: #67883]	erythromycin ChEBI				yes	50	µg/mL
[Ref.: #67883]	kanamycin ChEBI				yes	100	µg/mL
[Ref.: #67883]	ofloxacin ChEBI				yes	50	µg/mL
[Ref.: #67883]	spectinomycin ChEBI				yes	100	µg/mL
[Ref.: #67883]	streptomycin ChEBI				yes	100	µg/mL
[Ref.: #67883]	tetracycline ChEBI	yes	25	µg/mL

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #67884]		urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64520]	Sample type/isolated from	coastal surface water
[Ref.: #64520]	Geographic location (country and/or sea, region)	Northern Adriatic Sea
[Ref.: #64520]	Country	Slovenia
[Ref.: #64520]	Country ISO 3 Code	SVN
[Ref.: #64520]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Northern Adriatic coastal surface water sampled off Piran
[Ref.: #67770]	Country	Slovenia
[Ref.: #67770]	Country ISO 3 Code	SVN
[Ref.: #67770]	Continent	Europe

[Ref.: #67883]	Sample type/isolated from	coastal surface water (0.5m depth)
[Ref.: #67883]	Sampling date	2011-11
[Ref.: #67883]	Geographic location (country and/or sea, region)	Northern Adriatic Sea, coast of Piran
[Ref.: #67883]	Country	Slovenia
[Ref.: #67883]	Country ISO 3 Code	SVN
[Ref.: #67883]	Continent	Europe
[Ref.: #67883]	Geographic location	45.5180°/13.5680°
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Coast
#Environmental	#Aquatic	#Surface water

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MK139955 (>99% sequence identity) for Nitrosopumilus from Microbeatlas

Aquatic Samples	60
Soil Samples	2
Animal Samples
Plant Samples
Total Samples	62

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67883]	Nitrosopumilus adriaticus strain NF5 16S ribosomal RNA gene, partial sequence	MK139955	1471	ENA	1580092 tax ID
[Ref.: #67770]	Nitrosopumilus adriaticus strain NF5 chromosome, complete genome	CP011070	1803090	ENA	1580092 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67883]	Nitrosopumilus adriaticus NF5	GCA_000956175	complete	GenBank	1580092 tax ID
[Ref.: #66792]	Nitrosopumilus adriaticus NF5	2627854092	complete	JGI IMG/M	1580092 tax ID	*

[Ref.: #64520]	GC-content	33.4 mol%	sequence analysis
[Ref.: #67770]	GC-content	33.4 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nitrosopumilus adriaticus NF5 NCBI: GCA_000956175.1
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #64520]

Culture collection no.

DSM 106890, JCM 32270, NCIMB 15114

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nitrosopumilus adriaticus sp. nov. and Nitrosopumilus piranensis sp. nov., two ammonia-oxidizing archaea from the Adriatic Sea and members of the class Nitrososphaeria.	Bayer B, Vojvoda J, Reinthaler T, Reyes C, Pinto M, Herndl GJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003360	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64520

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106890

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#67883

Barbara Bayer, Jana Vojvoda, Thomas Reinthaler, Carolina Reyes, Maria Pinto, Gerhard J. Herndl: Nitrosopumilus adriaticus sp. nov. and Nitrosopumilus piranensis sp. nov., two ammonia-oxidizing archaea from the Adriatic Sea and members of the class Nitrososphaeria. IJSEM 69: 1892 - 1902 2019 ( DOI 10.1099/ijsem.0.003360 )

#67884

Zhendong Zhang, Qiangchuan Hou, Yurong Wang, Weicheng Li, Huijun Zhao, Zhihong Sun, Zhuang Guo: Lactobacillus zhachilii sp. nov., a lactic acid bacterium isolated from Zha-Chili. IJSEM 69: 2196 - 2201 2019 ( DOI 10.1099/ijsem.0.003362 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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