Psychrobacillus insolitus (DSM 5, ATCC 23299, CCM 2175)

Name: Psychrobacillus insolitus (DSM 5, ATCC 23299, CCM 2175)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1565

Type strain

Strain Designation W16B

Culture col. no. DSM 5 ATCC 23299 CCM 2175 NCIB 11433 NCIMB 11433 4 more

NCBI tax ID(s) 1461

Special Collections DSMZ CCUG CIP

History <- ATCC <- J.L. Stokes, W16B CIP <- 1988, DSM <- ATCC <- J.L. Stokes: strain W16B

Links

Psychrobacillus insolitus W16B is a bacterium that was isolated from soil.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 1124

LMG 7126,ATCC 23299,CCM 2175,CCUG 7420,DSM 5,NCIB 11433,LMG 17757,CIP 103268,NCIMB 11433,KCTC 3737,BCRC 11737,CCRC 11737,CCT 2470,HAMBI 477,KCTC 3854,NRRL B-3395,CGMCC 1.3683,CFBP 2978,VTT E-011789

@ref 20215

Last LPSN update 2026-02-09 11:18:08

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Caryophanaceae

Genus Psychrobacillus

Species Psychrobacillus insolitus

Full scientific name Psychrobacillus insolitus (Larkin and Stokes 1967) Krishnamurthi et al. 2011

Synonyms (1)

Bacillus insolitus

BacDive ID	Other strains from **Psychrobacillus insolitus** (2)	Type strain
1564	P. insolitus T16B, DSM 2272, ATCC 23300
145742	P. insolitus CCUG 29460, NRRL NRS-1517

Morphology

Colony morphology

2013

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
35520	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
2013	MARINE AGAR (DSMZ Medium 123)	Medium recipe at MediaDive	Name: MARINE AGAR (DSMZ Medium 123) Composition: NaCl 24.0 g/l Agar 15.0 g/l MgCl2 x 6 H2O 11.0 g/l Bacto peptone 5.0 g/l Na2SO4 4.0 g/l CaCl2 x 6 H2O 2.0 g/l Yeast extract 1.0 g/l KCl 0.7 g/l KBr 0.1 g/l SrCl2 x 6 H2O 0.04 g/l H3BO3 0.03 g/l NaSiO3 x 9 H2O 0.005 g/l NaF 0.003 g/l NH4NO3 0.002 g/l Fe(III)PO4 x 4 H2O 0.001 g/l Distilled water
120137	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2013	positive	growth	20-25
35520	positive	growth	15

Physiology and metabolism

Murein

@ref	Murein short key	Type
2013	A21.05	A4ß L-Orn-D-Glu

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	valine metabolism	100	9 of 9
66794	formaldehyde oxidation	100	3 of 3
66794	vitamin K metabolism	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ethanol fermentation	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	palmitate biosynthesis	100	22 of 22
66794	lipoate biosynthesis	100	5 of 5
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	butanoate fermentation	100	4 of 4
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	gluconeogenesis	100	8 of 8
66794	coenzyme A metabolism	100	4 of 4
66794	aerobactin biosynthesis	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	suberin monomers biosynthesis	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	leucine metabolism	92.31	12 of 13
66794	vitamin B1 metabolism	92.31	12 of 13
66794	propionate fermentation	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	heme metabolism	85.71	12 of 14
66794	propanol degradation	85.71	6 of 7
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	phenylalanine metabolism	84.62	11 of 13
66794	peptidoglycan biosynthesis	80	12 of 15
66794	Entner Doudoroff pathway	80	8 of 10
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	urea cycle	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	androgen and estrogen metabolism	75	12 of 16
66794	isoleucine metabolism	75	6 of 8
66794	flavin biosynthesis	73.33	11 of 15
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	lysine metabolism	71.43	30 of 42
66794	phenol degradation	70	14 of 20
66794	methionine metabolism	69.23	18 of 26
66794	alanine metabolism	68.97	20 of 29
66794	pyrimidine metabolism	68.89	31 of 45
66794	purine metabolism	68.09	64 of 94
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	pentose phosphate pathway	63.64	7 of 11
66794	proline metabolism	63.64	7 of 11
66794	non-pathway related	63.16	24 of 38
66794	tryptophan metabolism	63.16	24 of 38
66794	ketogluconate metabolism	62.5	5 of 8
66794	gallate degradation	60	3 of 5
66794	3-phenylpropionate degradation	60	9 of 15
66794	3-chlorocatechol degradation	60	3 of 5
66794	arachidonate biosynthesis	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	arginine metabolism	58.33	14 of 24
66794	lipid metabolism	58.06	18 of 31
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	degradation of sugar alcohols	56.25	9 of 16
66794	oxidative phosphorylation	54.95	50 of 91
66794	histidine metabolism	51.72	15 of 29
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	acetate fermentation	50	2 of 4
66794	pantothenate biosynthesis	50	3 of 6
66794	ribulose monophosphate pathway	50	1 of 2
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	resorcinol degradation	50	1 of 2
66794	starch degradation	50	5 of 10
66794	glutathione metabolism	50	7 of 14
66794	glycine metabolism	50	5 of 10
66794	quinate degradation	50	1 of 2
66794	biotin biosynthesis	50	2 of 4
66794	cysteine metabolism	50	9 of 18
66794	sulfate reduction	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	d-xylose degradation	45.45	5 of 11
66794	d-mannose degradation	44.44	4 of 9
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	polyamine pathway	43.48	10 of 23
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	vitamin B12 metabolism	41.18	14 of 34
66794	glycogen metabolism	40	2 of 5
66794	degradation of sugar acids	40	10 of 25
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	cholesterol biosynthesis	36.36	4 of 11
66794	cyanate degradation	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	ascorbate metabolism	31.82	7 of 22
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	degradation of pentoses	28.57	8 of 28
66794	degradation of hexoses	27.78	5 of 18
66794	metabolism of disaccharids	27.27	3 of 11
66794	CMP-KDO biosynthesis	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	nitrate assimilation	22.22	2 of 9
66794	allantoin degradation	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9
66794	arachidonic acid metabolism	22.22	4 of 18

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
2013	soil
44989	Soil
120137	Environment, Soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2013	1	Risk group (German classification) According to TRBA 466
120137	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM325415v1 assembly for Psychrobacillus insolitus DSM 5	scaffold	GCA_003254155	1461.4	2754412937	1461	70.99

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	B.insolitus 16S ribosomal RNA	X60642	1431		1461
2013	Bacillus insolitus partial 16S rRNA gene, type strain DSM 5T	AM980508	1502		1461

GC content

@ref	GC-content (mol%)	Method
2013	35.8	Buoyant density centrifugation (BD)
2013	36.1	Buoyant density centrifugation (BD)
2013	35.9	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Genome and pan-genome analysis of a new exopolysaccharide-producing bacterium Pyschrobacillus sp. isolated from iron ores deposit and insights into iron uptake.	Najjari A, Jabberi M, Cherif SF, Cherif A, Ouzari HI, Linares-Pasten JA, Sghaier H.	Front Microbiol	10.3389/fmicb.2024.1440081	2024
Phylogeny	Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov.	Krishnamurthi S, Ruckmani A, Pukall R, Chakrabarti T	Syst Appl Microbiol	10.1016/j.syapm.2010.06.003	2010
Phylogeny	Psychrobacillus glaciei sp. nov., a psychrotolerant species isolated from an Antarctic iceberg.	Choi JY, Lee PC	Int J Syst Evol Microbiol	10.1099/ijsem.0.003998	2020
Phylogeny	Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus).	Shen Y, Fu Y, Yu Y, Zhao J, Li J, Li Y, Wang X, Zhang J, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.002315	2017
Phylogeny	Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls.	Abd El-Rahman HA, Fritze D, Sproer C, Claus D	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2127	2002

References

#2013	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#35520	; Curators of the CIP;
#44989	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 7420
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#120137	Collection of Institut Pasteur ; Curators of the CIP; CIP 103268
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Psychrobacillus insolitus (DSM 5, ATCC 23299, CCM 2175)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Murein

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003254155

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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