Strain identifier

BacDive ID: 1565

Type strain: Yes

Species: Psychrobacillus insolitus

Strain Designation: W16B

Strain history: <- ATCC <- J.L. Stokes, W16B

NCBI tax ID(s): 1461 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7 (current version):
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7 (current version)

General

@ref: 2013

BacDive-ID: 1565

DSM-Number: 5

keywords: Bacteria, 16S sequence

description: Psychrobacillus insolitus W16B is a bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1461
  • Matching level: species

strain history: <- ATCC <- J.L. Stokes, W16B

doi: 10.13145/bacdive1565.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Psychrobacillus
  • species: Psychrobacillus insolitus
  • full scientific name: Psychrobacillus insolitus (Larkin and Stokes 1967) Krishnamurthi et al. 2011
  • synonyms

    • @ref: 20215
    • synonym: Bacillus insolitus

@ref: 2013

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Caryophanaceae

genus: Psychrobacillus

species: Psychrobacillus insolitus

full scientific name: Psychrobacillus insolitus (Larkin and Stokes 1967) Krishnamurthi et al. 2011

strain designation: W16B

type strain: yes

Morphology

colony morphology

  • @ref: 2013
  • incubation period: >14 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35520MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
2013MARINE AGAR (DSMZ Medium 123)yesName: MARINE AGAR (DSMZ Medium 123) Composition: NaCl 24.0 g/l Agar 15.0 g/l MgCl2 x 6 H2O 11.0 g/l Bacto peptone 5.0 g/l Na2SO4 4.0 g/l CaCl2 x 6 H2O 2.0 g/l Yeast extract 1.0 g/l KCl 0.7 g/l KBr 0.1 g/l SrCl2 x 6 H2O 0.04 g/l H3BO3 0.03 g/l NaSiO3 x 9 H2O 0.005 g/l NaF 0.003 g/l NH4NO3 0.002 g/l Fe(III)PO4 x 4 H2O 0.001 g/l Distilled waterhttps://bacmedia.dsmz.de/medium/123

culture temp

@refgrowthtypetemperaturerange
2013positivegrowth20-25
35520positivegrowth15psychrophilic

Physiology and metabolism

murein

  • @ref: 2013
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

Isolation, sampling and environmental information

isolation

@refsample type
2013soil
44989Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 2013
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus insolitus clone GM-1_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478084495ena1461
20218Pseudomonas aeruginosa strain DSM-5 16S ribosomal RNA gene, partial sequenceJQ756457674ena287
20218Vibrio sp. DSM5 16S ribosomal RNA gene, partial sequenceKC439212910ena1343244
20218B.insolitus 16S ribosomal RNAX606421431ena1461
2013Bacillus insolitus partial 16S rRNA gene, type strain DSM 5TAM9805081502ena1461

GC content

@refGC-contentmethod
201335.8Buoyant density centrifugation (BD)
201336.1Buoyant density centrifugation (BD)
201335.9thermal denaturation, midpoint method (Tm)

External links

@ref: 2013

culture collection no.: DSM 5, ATCC 23299, CCM 2175, NCIB 11433, NCIMB 11433, CCUG 7420

straininfo link

@refpassport
20218http://www.straininfo.net/strains/4656
20218http://www.straininfo.net/strains/4653
20218http://www.straininfo.net/strains/4654
20218http://www.straininfo.net/strains/210042

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20650590Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov.Krishnamurthi S, Ruckmani A, Pukall R, Chakrabarti TSyst Appl Microbiol10.1016/j.syapm.2010.06.0032010Bacillus/*classification/cytology/genetics/physiology, *Bacterial Typing Techniques, Base Composition, Base Sequence, Carbohydrates/chemistry, Chemotaxis, Cold Temperature, DNA, Bacterial/genetics, *Gram-Positive Endospore-Forming Rods/classification/cytology/genetics/physiology, Lipids/chemistry, Molecular Sequence Data, Peptidoglycan/chemistry, Phenotype, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA, Species Specificity, Terpenes/chemistryGenetics
Phylogeny28933325Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus).Shen Y, Fu Y, Yu Y, Zhao J, Li J, Li Y, Wang X, Zhang J, Xiang WInt J Syst Evol Microbiol10.1099/ijsem.0.0023152017Animals, Ants/*microbiology, Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny31985396Psychrobacillus glaciei sp. nov., a psychrotolerant species isolated from an Antarctic iceberg.Choi JY, Lee PCInt J Syst Evol Microbiol10.1099/ijsem.0.0039982020Antarctic Regions, Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2013Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35520Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15047
44989Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7420)https://www.ccug.se/strain?id=7420
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880