Strain identifier
BacDive ID: 1565
Type strain: 
Species: Psychrobacillus insolitus
Strain Designation: W16B
Strain history: CIP <- 1988, DSM <- ATCC <- J.L. Stokes: strain W16B
NCBI tax ID(s): 1461 (species)
version 8.1 (current version)
General
@ref: 2013
BacDive-ID: 1565
DSM-Number: 5
keywords: 16S sequence, Bacteria
description: Psychrobacillus insolitus W16B is a bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 1461
- Matching level: species
strain history
| @ref | history |
|---|---|
| 2013 | <- ATCC <- J.L. Stokes, W16B |
| 120137 | CIP <- 1988, DSM <- ATCC <- J.L. Stokes: strain W16B |
doi: 10.13145/bacdive1565.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Caryophanaceae
- genus: Psychrobacillus
- species: Psychrobacillus insolitus
- full scientific name: Psychrobacillus insolitus (Larkin and Stokes 1967) Krishnamurthi et al. 2011
synonyms
- @ref: 20215
- synonym: Bacillus insolitus
@ref: 2013
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Caryophanaceae
genus: Psychrobacillus
species: Psychrobacillus insolitus
full scientific name: Psychrobacillus insolitus (Larkin and Stokes 1967) Krishnamurthi et al. 2011
strain designation: W16B
type strain: yes
Morphology
colony morphology
- @ref: 2013
- incubation period: >14 days
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 35520 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
| 2013 | MARINE AGAR (DSMZ Medium 123) | yes | Name: MARINE AGAR (DSMZ Medium 123) Composition: NaCl 24.0 g/l Agar 15.0 g/l MgCl2 x 6 H2O 11.0 g/l Bacto peptone 5.0 g/l Na2SO4 4.0 g/l CaCl2 x 6 H2O 2.0 g/l Yeast extract 1.0 g/l KCl 0.7 g/l KBr 0.1 g/l SrCl2 x 6 H2O 0.04 g/l H3BO3 0.03 g/l NaSiO3 x 9 H2O 0.005 g/l NaF 0.003 g/l NH4NO3 0.002 g/l Fe(III)PO4 x 4 H2O 0.001 g/l Distilled water | https://mediadive.dsmz.de/medium/123 |
| 120137 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 2013 | positive | growth | 20-25 | |
| 35520 | positive | growth | 15 | psychrophilic |
Physiology and metabolism
murein
- @ref: 2013
- murein short key: A21.05
- type: A4ß L-Orn-D-Glu
Isolation, sampling and environmental information
isolation
| @ref | sample type |
|---|---|
| 2013 | soil |
| 44989 | Soil |
| 120137 | Environment, Soil |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 2013 | 1 | Risk group (German classification) |
| 120137 | 1 | Risk group (French classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Bacillus insolitus clone GM-1_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | AF478084 | 495 | ena | 1461 |
| 20218 | Pseudomonas aeruginosa strain DSM-5 16S ribosomal RNA gene, partial sequence | JQ756457 | 674 | ena | 287 |
| 20218 | Vibrio sp. DSM5 16S ribosomal RNA gene, partial sequence | KC439212 | 910 | ena | 1343244 |
| 20218 | B.insolitus 16S ribosomal RNA | X60642 | 1431 | ena | 1461 |
| 2013 | Bacillus insolitus partial 16S rRNA gene, type strain DSM 5T | AM980508 | 1502 | ena | 1461 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 2013 | 35.8 | Buoyant density centrifugation (BD) |
| 2013 | 36.1 | Buoyant density centrifugation (BD) |
| 2013 | 35.9 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 2013
culture collection no.: DSM 5, ATCC 23299, CCM 2175, NCIB 11433, NCIMB 11433, CCUG 7420, CIP 103268, LMG 7126
straininfo link
- @ref: 71210
- straininfo: 1124
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 12508879 | Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. | Abd El-Rahman HA, Fritze D, Sproer C, Claus D | Int J Syst Evol Microbiol | 10.1099/00207713-52-6-2127 | 2002 | Bacillus/*classification/genetics/*metabolism, Base Composition, Cell Wall/metabolism, Cold Temperature, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Ornithine/*metabolism, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic | Metabolism |
| Phylogeny | 20650590 | Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov. | Krishnamurthi S, Ruckmani A, Pukall R, Chakrabarti T | Syst Appl Microbiol | 10.1016/j.syapm.2010.06.003 | 2010 | Bacillus/*classification/cytology/genetics/physiology, *Bacterial Typing Techniques, Base Composition, Base Sequence, Carbohydrates/chemistry, Chemotaxis, Cold Temperature, DNA, Bacterial/genetics, *Gram-Positive Endospore-Forming Rods/classification/cytology/genetics/physiology, Lipids/chemistry, Molecular Sequence Data, Peptidoglycan/chemistry, Phenotype, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA, Species Specificity, Terpenes/chemistry | Genetics |
| Phylogeny | 28933325 | Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus). | Shen Y, Fu Y, Yu Y, Zhao J, Li J, Li Y, Wang X, Zhang J, Xiang W | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002315 | 2017 | Animals, Ants/*microbiology, Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 31985396 | Psychrobacillus glaciei sp. nov., a psychrotolerant species isolated from an Antarctic iceberg. | Choi JY, Lee PC | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003998 | 2020 | Antarctic Regions, Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2013 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 35520 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15047 | ||||
| 44989 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 7420) | https://www.ccug.se/strain?id=7420 | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 71210 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1124.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 120137 | Curators of the CIP | Collection of Institut Pasteur (CIP 103268) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103268 |