Name: Streptomyces malachitofuscus DSM 40332
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 15392

Type strain:

Species: Streptomyces malachitofuscus

Culture col. no.: DSM 40332, ATCC 25471, CBS 881.69, IFO 13059, INA 739, ISP 5332, NBRC 13059, RIA 1251, JCM 4493, BCRC 15143, CGMCC 4.1743, NRRL B-12273, VKM Ac-1850

Strain history: KCC S-0493 <-- IFO 13059 <-- SAJ <-- ISP 5332 <-- INA 739.

NCBI tax ID(s): 67322 (species)

SI-ID 389335

ATCC 25471, IFO 13059, DSM 40332, JCM 4493, NRRL B-12273, VKM Ac-1850, CBS 881.69, NBRC 13059, LMG 20067, BCRC 15143, CCRC 15143, CGMCC 4.1743

Section

Streptomyces malachitofuscus DSM 40332 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces malachitofuscus**
	Full Scientific Name (LPSN)	Streptomyces malachitofuscus (ex Preobrazhenskaya et al. 1964) Preobrazhenskaya and Terekhova 1986

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %90.234

[Ref.: #69256]	Colony color	Brown beige (1011), May green (6017), Ochre yellow (1024)
[Ref.: #69256]	Cultivation medium used	ISP 4

[Ref.: #69256]	Colony color	Beige (1001), Light ivory (1015)
[Ref.: #69256]	Cultivation medium used	ISP 5

[Ref.: #69256]	Colony color	Ivory (1014)
[Ref.: #69256]	Cultivation medium used	ISP 2

[Ref.: #69256]	Colony color	Ivory (1014), Brown beige (1011), May green (6017), Reseda green (6011)
[Ref.: #69256]	Cultivation medium used	ISP 3

[Ref.: #69256]	Colony color	Ochre yellow (1024), Brown beige (1011)
[Ref.: #69256]	Cultivation medium used	suter without tyrosine

[Ref.: #69256]	Colony color	Ochre yellow (1024), Ivory (1014)
[Ref.: #69256]	Cultivation medium used	ISP 7

[Ref.: #69256]	Colony color	Sand yellow (1002)
[Ref.: #69256]	Cultivation medium used	ISP 6

[Ref.: #69256]	Colony color	Sepia brown (8014)
[Ref.: #69256]	Cultivation medium used	suter with tyrosine

[Ref.: #69256]	Pigment production	no
[Ref.: #69256]	Pigment name	Melanin
[Ref.: #69256]	Pigment production	yes
[Ref.: #69256]	Pigmentation color	Olive brown (8008), sand yellow (1002), black red (3007), ochre brown (8001), ochre yellow (1024)
[Ref.: #69256]	Pigment name	soluble pigment

[Ref.: #69256]	Multicellular complex forming ability	yes
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Multicellular complex color	Grey white (9002)
[Ref.: #69256]	Medium Name (multicellularity)	ISP 2

[Ref.: #69256]	Multicellular complex forming ability	yes
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Multicellular complex color	Signal white (9003), agate grey (7038)
[Ref.: #69256]	Medium Name (multicellularity)	ISP 3

[Ref.: #69256]	Multicellular complex forming ability	no
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Medium Name (multicellularity)	ISP 4

[Ref.: #69256]	Multicellular complex forming ability	yes
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Multicellular complex color	Cream (9001)
[Ref.: #69256]	Medium Name (multicellularity)	ISP 5

[Ref.: #69256]	Multicellular complex forming ability	no
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Medium Name (multicellularity)	ISP 6

[Ref.: #69256]	Multicellular complex forming ability	yes
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Multicellular complex color	Traffic white (9016)
[Ref.: #69256]	Medium Name (multicellularity)	ISP 7

[Ref.: #69256]	Multicellular complex forming ability	yes
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Multicellular complex color	Traffic white (9016)
[Ref.: #69256]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69256]	Multicellular complex forming ability	yes
[Ref.: #69256]	Multicellular complex name	Aerial mycelium
[Ref.: #69256]	Multicellular complex color	Grey white (9002)
[Ref.: #69256]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69256]

Caption:

(SSM+T, SSM-T)

[Ref.: #69256]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69256]

Caption:

(SSM+T, SSM-T)

[Ref.: #69256]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #9464]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #9464]

Culture medium growth

[Ref.: #9464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #9464]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #9464]	growth	28 °C
[Ref.: #18584]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #9464]	Temperature range	mesophilic
[Ref.: #18584]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69256]		NaCl		growth	0-7.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69256]	arabinose ChEBI	+	growth
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #69256]	cellulose ChEBI	-	growth
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69256]	fructose ChEBI	+	growth
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E, API Coryne
[Ref.: #69256]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69256]	inositol ChEBI	+	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69256]	mannose ChEBI	+	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #69256]	raffinose ChEBI	-	growth
[Ref.: #69256]	rhamnose ChEBI	+	growth
[Ref.: #69256]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69256]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	yes	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E, API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18584]	142	+	+	+	+	+	+	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69256]	3750	-	-	-	+	-	-	-	-	-	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69256]	5934	not determinedn.d.	+	+/-	+	-	+	-	-	-	-	+	+	-	+	-	+	-	+	+	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18584]	Biosafety level	1	German classification
[Ref.: #9464]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Streptomyces malachitofuscus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4493	D44185	120	ENA	67322 tax ID	*
[Ref.: #20218]	Streptomyces malachitofuscus 16S rRNA gene, type strain LMG 20067	AJ781347	1469	ENA	67322 tax ID	*
[Ref.: #20218]	Streptomyces malachitofuscus gene for 16S rRNA, partial sequence, strain: NBRC 13059	AB184282	1469	ENA	67322 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptomyces malachitofuscus JCM 4493	GCA_014650055	scaffold	GenBank	67322 tax ID	*
[Ref.: #66792]	Streptomyces malachitofuscus strain JCM 4493	67322.3	wgs	PATRIC	67322 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptomyces malachitofuscus JCM 4493 NCBI: GCA_014650055.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptomyces malachitofuscus JCM 4493 NCBI: GCA_014650055.1
[Ref.: #69480]	flagellated	no	97.544	no
[Ref.: #69480]	gram-positive	yes	89.002	no
[Ref.: #69480]	anaerobic	no	98.896	no
[Ref.: #69480]	aerobic	yes	92.495	no
[Ref.: #69480]	halophile	no	90.656	no
[Ref.: #69480]	spore-forming	yes	96.32	no
[Ref.: #69480]	glucose-util	yes	91.18	yes
[Ref.: #69480]	thermophile	no	98.1	yes
[Ref.: #69480]	motile	no	94.065	no
[Ref.: #69480]	glucose-ferment	no	91.42	no

Availability in culture collections External links

[Ref.: #9464]

Culture collection no.

DSM 40332, ATCC 25471, CBS 881.69, IFO 13059, INA 739, ISP 5332, NBRC 13059, RIA 1251, JCM 4493, BCRC 15143, CGMCC 4.1743, NRRL B-12273, VKM Ac-1850

[Ref.: #84482]

SI-ID 389335

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines.	Law JW, Ser HL, Duangjai A, Saokaew S, Bukhari SI, Khan TM, Ab Mutalib NS, Chan KG, Goh BH, Lee LH	Front Microbiol	10.3389/fmicb.2017.00877	2017	*

References

#9464

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40332

#18584

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69256

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#84482

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID389335.1 )

* These data were automatically processed and therefore are not curated

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Streptomyces malachitofuscus JCM 4493

Streptomyces malachitofuscus JCM 4493

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