Name: Virgibacillus kekensis YIM-kkny16
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1488

Type strain:

Species: Virgibacillus kekensis

Strain Designation: YIM-kkny16

Culture col. no.: DSM 17056, CGMCC 1.6298, JCM 16510

Strain history: DSM 17056 <-- X. L. Cui YIM kkny16.

NCBI tax ID(s): 202261 (species)

SI-ID 343439

CGMCC 1.6298, DSM 17056, JCM 16510

Section

Virgibacillus kekensis YIM-kkny16 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from salt lake, mud samples.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
mesophilic
moderately halophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Virgibacillus*
	Species	**Virgibacillus kekensis**
	Full Scientific Name (LPSN)	Virgibacillus kekensis Chen et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #23058]	Gram stain	positive

[Ref.: #23058]	Cell length	2.0-3.0 µm

[Ref.: #23058]	Cell width	0.3-0.5 µm

[Ref.: #23058]	Cell shape	rod-shaped

[Ref.: #23058]	Motility	yes

[Ref.: #23058]	Colony size	2.0-3.0 mm
[Ref.: #23058]	Colony color	creamy grey
[Ref.: #23058]	Colony shape	circular
[Ref.: #23058]	Incubation period	3 days
[Ref.: #23058]	Cultivation medium used	MA supplemented with 10 % (w/v) NaCl

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6725]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6725]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #6725]

Culture medium composition

expand / minimize

[Ref.: #23058]

Culture medium

ISP medium 2 agar

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Culture medium growth

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Culture medium

MA supplemented with 10 % (w/v) NaCl

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

trypticase soy agar (BBL)

[Ref.: #23058]

Culture medium growth

		Temperature
[Ref.: #6725]	growth	30 °C
[Ref.: #23058]	growth	10.0-50.0 °C
[Ref.: #23058]	optimum	37.0 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #6725]	Temperature range	mesophilic
[Ref.: #23058]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

		pH
[Ref.: #23058]	optimum	7.0
[Ref.: #23058]	growth	6.0-10.0
[Ref.: #23058]	growth	10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23058]

Oxygen tolerance

obligate aerobe

[Ref.: #23058]	Spore description	ellipsoidal,terminal,in swollen sporangia
[Ref.: #23058]	Type of spore	endospore
[Ref.: #23058]	Ability of spore formation	yes

[Ref.: #23058]

Halophily / tolerance level

moderately halophilic

	Salt		Salt conc.
[Ref.: #23058]	NaCl	growth	0.0-25.0	%(w/v)
[Ref.: #23058]	NaCl	optimum	10.0	%(w/v)
[Ref.: #23058]	NaCl	growth	0.0	%(w/v)
[Ref.: #23058]	NaCl	growth	20.0	%(w/v)

[Ref.: #23058]	Murein short key	A31
[Ref.: #23058]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23058]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23058]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23058]	amygdalin ChEBI	-	builds acid from
[Ref.: #23058]	arbutin ChEBI	-	builds acid from
[Ref.: #23058]	casein	-	hydrolysis
[Ref.: #23058]	cellobiose ChEBI	-	builds acid from
[Ref.: #23058]	cellulose ChEBI	-	hydrolysis
[Ref.: #23058]	chitin ChEBI	-	hydrolysis
[Ref.: #23058]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23058]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23058]	D-fructose ChEBI	-	builds acid from
[Ref.: #23058]	D-fucose ChEBI	-	builds acid from
[Ref.: #23058]	D-galactose ChEBI	-	builds acid from
[Ref.: #23058]	D-glucose ChEBI	+	builds acid from
[Ref.: #23058]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23058]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23058]	D-mannose ChEBI	+	builds acid from
[Ref.: #23058]	D-ribose ChEBI	-	builds acid from
[Ref.: #23058]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23058]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23058]	D-xylose ChEBI	-	builds acid from
[Ref.: #23058]	erythritol ChEBI	-	builds acid from
[Ref.: #23058]	esculin ChEBI	-	builds acid from
[Ref.: #23058]	esculin ChEBI	-	hydrolysis
[Ref.: #23058]	galactitol ChEBI	-	builds acid from
[Ref.: #23058]	gelatin ChEBI	-	hydrolysis
[Ref.: #23058]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23058]	gluconate ChEBI	-	builds acid from
[Ref.: #23058]	glucose ChEBI	-	fermentation
[Ref.: #23058]	glycerol ChEBI	-	builds acid from
[Ref.: #23058]	glycogen ChEBI	+	builds acid from
[Ref.: #23058]	inulin ChEBI	-	builds acid from
[Ref.: #23058]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23058]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23058]	L-fucose ChEBI	-	builds acid from
[Ref.: #23058]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23058]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23058]	L-xylose ChEBI	-	builds acid from
[Ref.: #23058]	lactose ChEBI	-	builds acid from
[Ref.: #23058]	maltose ChEBI	+	builds acid from
[Ref.: #23058]	melezitose ChEBI	-	builds acid from
[Ref.: #23058]	melibiose ChEBI	-	builds acid from
[Ref.: #23058]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23058]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23058]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #23058]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23058]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23058]	nitrate ChEBI	+	reduction
[Ref.: #23058]	raffinose ChEBI	-	builds acid from
[Ref.: #23058]	ribitol ChEBI	-	builds acid from
[Ref.: #23058]	salicin ChEBI	+	builds acid from
[Ref.: #23058]	starch ChEBI	+	builds acid from
[Ref.: #23058]	starch ChEBI	+	hydrolysis
[Ref.: #23058]	sucrose ChEBI	-	builds acid from
[Ref.: #23058]	trehalose ChEBI	-	builds acid from
[Ref.: #23058]	trehalose ChEBI	+	builds acid from
[Ref.: #23058]	tryptophan ChEBI	-	energy source
[Ref.: #23058]	turanose ChEBI	-	builds acid from
[Ref.: #23058]	tween 20 ChEBI	-	hydrolysis
[Ref.: #23058]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23058]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23058]	ampicillin ChEBI	yes	30	µg
[Ref.: #23058]	chloramphenicol ChEBI	yes	30	µg
[Ref.: #23058]	gentamicin ChEBI				yes	10	µg
[Ref.: #23058]	kanamycin ChEBI				yes	30	µg
[Ref.: #23058]	lincomycin ChEBI	yes	2	µg
[Ref.: #23058]	nalidixic acid ChEBI				yes	20	µg
[Ref.: #23058]	novobiocin ChEBI	yes	30	µg
[Ref.: #23058]	polymyxin b ChEBI	yes	30	µg
[Ref.: #23058]	rifampicin ChEBI	yes	5	µg
[Ref.: #23058]	streptomycin ChEBI				yes	10	µg
[Ref.: #23058]	tetracycline ChEBI	yes	30	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23058]	acetoin ChEBI	no
[Ref.: #23058]	hydrogen sulfide ChEBI	no
[Ref.: #23058]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #23058]	acetoin ChEBI	-
[Ref.: #23058]	glucose ChEBI		-
[Ref.: #23058]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #23058]	acid phosphatase	+	3.1.3.2
[Ref.: #23058]	alkaline phosphatase	+	3.1.3.1
[Ref.: #23058]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #23058]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23058]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23058]	alpha-glucosidase	+	3.2.1.20
[Ref.: #23058]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23058]	beta-galactosidase	-	3.2.1.23
[Ref.: #23058]	beta-glucosidase	+	3.2.1.21
[Ref.: #23058]	beta-glucuronidase	+	3.2.1.31
[Ref.: #23058]	catalase	+	1.11.1.6
[Ref.: #23058]	cystine arylamidase	-	3.4.11.3
[Ref.: #23058]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23058]	esterase (C 4)	+
[Ref.: #23058]	esterase lipase (C 8)	+
[Ref.: #23058]	leucine arylamidase	+	3.4.11.1
[Ref.: #23058]	lipase (C 14)	-
[Ref.: #23058]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23058]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #23058]	trypsin	+	3.4.21.4
[Ref.: #23058]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23058]	urease	-	3.5.1.5
[Ref.: #23058]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6725]	Sample type/isolated from	salt lake, mud samples
[Ref.: #6725]	Country	China
[Ref.: #6725]	Country ISO 3 Code	CHN
[Ref.: #6725]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Saline mud from the Keke salt lake in the Qaidam basin
[Ref.: #67770]	Geographic location (country and/or sea, region)	Qinghai Province, north-west China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #23058]	Geographic location (country and/or sea, region)	Keke salt lake in the Qaidam Basin
[Ref.: #23058]	Enrichment culture	Difco marine agar 2216 (MA; pH 7.2)
[Ref.: #23058]	Enrichment culture duration	14 days
[Ref.: #23058]	Enrichment culture temperature	28.0 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Saline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6725]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6725]	Virgibacillus kekensis strain YIM kkny16 16S ribosomal RNA gene, partial sequence	AY121439	1562	ENA	202261 tax ID

[Ref.: #6725]	GC-content	41.8 mol%
[Ref.: #67770]	GC-content	41.8 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #6725]

Culture collection no.

DSM 17056, CGMCC 1.6298, JCM 16510

[Ref.: #71134]

SI-ID 343439

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Melghiribacillus thermohalophilus gen. nov., sp. nov., a novel filamentous, endospore-forming, thermophilic and halophilic bacterium.	Addou NA, Schumann P, Sproer C, Ben Hania W, Hacene H, Fauque G, Cayol JL, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijs.0.000075	2015	*
Phylogeny	Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China.	Chen YG, Cui XL, Fritze D, Chai LH, Schumann P, Wen ML, Wang YX, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.65365-0	2008	*

References

#6725

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17056

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23058

Yi-Guang Chen,Xiao-Long Cui,Dagmar Fritze,Li-Hong Chai,Peter Schumann,Meng-Liang Wen,Yong-Xia Wang,Li-Hua Xu,Cheng-Lin Jiang: Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. IJSEM 58: 647 - 653 2008 ( DOI 10.1099/ijs.0.65365-0 , PubMed 18319472 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#71134

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID343439.1 )

* These data were automatically processed and therefore are not curated

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