Name: Streptococcus difficilis ND 2-22
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 14714

Type strain:

Species: Streptococcus difficilis

Strain Designation: ND 2-22

Culture col. no.: DSM 16828, ATCC 51487, CIP 103768, LMG 15977, JCM 32209

Strain history: CIP <- 1993, H. Bercovier, Jerusalem Univ., Jerusalem, Israel: strain ND 2-22, Streptococcus difficile

NCBI tax ID(s): 1311 (species)

SI-ID 8817

LMG 15977, ATCC 51487, CIP 103768, DSM 16828

Special Collections

Section

Streptococcus difficilis ND 2-22 is a microaerophile, Gram-positive human pathogen that was isolated from Fish, brain.

Gram-positive
human pathogen
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Streptococcaceae*
	Genus	*Streptococcus*
	Species	Streptococcus difficilis
	Full Scientific Name (LPSN)	Streptococcus difficilis corrig. Eldar et al. 1995

	Synonym	**Streptococcus agalactiae**
	Synonym	Streptococcus difficile

Information on morphological and physiological properties Morphology

[Ref.: #116125]	Gram stain	positive

[Ref.: #116125]	Cell shape	coccus-shaped

[Ref.: #116125]	Motility	no

[Ref.: #6620]	Type of hemolysis	gamma
[Ref.: #6620]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6620]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #6620]

Culture medium growth

[Ref.: #6620]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6620]

Culture medium composition

expand / minimize

[Ref.: #6620]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #6620]

Culture medium growth

[Ref.: #6620]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6620]

Culture medium composition

expand / minimize

[Ref.: #38467]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #38467]

Culture medium growth

[Ref.: #38467]

Culture medium composition

expand / minimize

[Ref.: #116125]

Culture medium

CIP Medium 29

[Ref.: #116125]

Culture medium growth

[Ref.: #116125]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6620]		growth	30 °C
[Ref.: #38467]		growth	30 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #116125]		growth	30 °C
[Ref.: #116125]	no	growth	10 °C
[Ref.: #116125]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6620]	Oxygen tolerance	microaerophile
[Ref.: #116125]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #116125]		NaCl	no	growth	6.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68381]	D-ribose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116125]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #116125]	nitrate ChEBI	-	reduction
[Ref.: #116125]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #116125]		acetoin ChEBI	+
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	+		* from API rID32STR
[Ref.: #116125]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68381]	alkaline phosphatase	+	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #116125]	beta-galactosidase	-	3.2.1.23
[Ref.: #68381]	beta-galactosidase	-	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	-	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	+	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #6620]	catalase	+	1.11.1.6
[Ref.: #116125]	catalase	-	1.11.1.6
[Ref.: #6620]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #116125]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #116125]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #116125]	9391	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	+	-	-	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #6620]	10321	+	-	-	+	-	+	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	+	-	-	+	-	-	-	-	-	-
[Ref.: #6620]	10320	+	-	-	+	-	+	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	+	-	-	+/-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6620]	Sample type/isolated from	Fish, brain
[Ref.: #6620]	Country	Israel
[Ref.: #6620]	Country ISO 3 Code	ISR
[Ref.: #6620]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Brain of a fish (Tilapia)
[Ref.: #67770]	Host species	Tilapia
[Ref.: #67770]	Country	Israel
[Ref.: #67770]	Country ISO 3 Code	ISR
[Ref.: #67770]	Continent	Asia

[Ref.: #116125]	Sample type/isolated from	Other, Fish, brain
[Ref.: #116125]	Country	Israel
[Ref.: #116125]	Country ISO 3 Code	ISR
[Ref.: #116125]	Continent	Asia
[Ref.: #116125]	Isolation date	1988
* marker position based on {}

Isolation sources categories

#Host	#Fishes	-
#Host Body-Site	#Organ	#Brain

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB112407 (>99% sequence identity) for Streptococcus agalactiae subclade from Microbeatlas

Aquatic Samples	3036
Soil Samples	2237
Animal Samples	61262
Plant Samples	722
Total Samples	67257

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6620]	Pathogenicity (human)	yes
[Ref.: #6620]	Pathogenicity (animal)	yes
[Ref.: #6620]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #116125]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Streptococcus agalactiae strain ATCC 51487 16S ribosomal RNA genes, partial sequence	AH006153	475	GenBank ENA	1311 tax ID	*
[Ref.: #20218]	Streptococcus difficile 16S ribosomal RNA gene, partial sequence; tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF064441	792	GenBank ENA	1311 tax ID	*
[Ref.: #20218]	Streptococcus difficilis gene for 16S rRNA, partial sequence	AB112407	1451	GenBank ENA	1311 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptococcus agalactiae strain 22-Feb	1261567.3	complete	PATRIC	1311 tax ID	*

[Ref.: #67770]

GC-content

36.6-38 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptococcus agalactiae strain 22-Feb PATRIC: 1261567.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptococcus agalactiae strain 22-Feb PATRIC: 1261567.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	86.522	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	90.585	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.53	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	94.841	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.942	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	88.5	no

Availability in culture collections External links

[Ref.: #6620]

Culture collection no.

DSM 16828, ATCC 51487, CIP 103768, LMG 15977, JCM 32209

[Ref.: #83833]

SI-ID 8817

Literature:

	Title	Authors	Journal	DOI	Year
	Development of a bacterial challenge test for gnotobiotic Nile tilapia Oreochromis niloticus larvae.	Situmorang ML, Dierckens K, Mlingi FT, Van Delsen B, Bossier P	Dis Aquat Organ	10.3354/dao02721	2014	*

References

#6620

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16828

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38467 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83833

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8817.1 )

#116125 Collection of Institut Pasteur ; Curators of the CIP; CIP 103768
* These data were automatically processed and therefore are not curated

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Streptococcus agalactiae strain 22-Feb

Streptococcus agalactiae strain 22-Feb

Change proposal

Change proposal