Streptococcus difficilis (DSM 16828, ATCC 51487, CIP 103768)

Name: Streptococcus difficilis (DSM 16828, ATCC 51487, CIP 103768)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14714

Type strain

Strain Designation ND 2-22

Culture col. no. DSM 16828 ATCC 51487 CIP 103768 LMG 15977 JCM 32209 1 more

NCBI tax ID(s) 1311

Special Collections DSMZ JCM CIP

History <- CIP; CIP 103768 <- H. Bercovier, Univ. Jerusalem; ND 2-22 (Streptococcus difficilis) LMG 15977 <-- CIP 103768 <-- H. Bercovier; Jerusalem Univ., Israel; ND 2-22. CIP <- 1993, H. Bercovier, Jerusalem Univ., Jerusalem, Israel: strain ND 2-22, Streptococcus difficile

Links

Streptococcus difficilis ND 2-22 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from Fish, brain.

Gram-positive coccus-shaped microaerophile mesophilic human pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 8817

LMG 15977,ATCC 51487,CIP 103768,JCM 32209,DSM 16828

@ref 20215

Last LPSN update 2026-02-09 11:21:29

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Streptococcaceae

Genus Streptococcus

Species Streptococcus difficilis

Full scientific name Streptococcus difficilis corrig. Eldar et al. 1995

Synonyms (2)

Streptococcus difficile
Streptococcus agalactiae

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116125	positive	coccus-shaped

Colony morphology

@ref	Type of hemolysis	Incubation period
6620	gamma	1-2 days
116125

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6620	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6620	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38467	MEDIUM 29- Brain heart agar		Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
116125	CIP Medium 29	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6620	positive	growth	30	mesophilic
38467	positive	growth	30	mesophilic
67770	positive	growth	37	mesophilic
116125	positive	growth	30	mesophilic
116125	negative	growth	10
116125	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6620	microaerophile
116125	facultative anaerobe
125439	obligate aerobe	94.4

Halophily

@ref	Salt	Growth	Tested relation	Concentration
116125	NaCl		growth	6.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68381	29016 ChEBI	arginine	+	hydrolysis	from API rID32STR
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
116125	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68381	606565 ChEBI	hippurate	+	hydrolysis	from API rID32STR
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68381	17716 ChEBI	lactose	-	builds acid from	from API rID32STR
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68381	28053 ChEBI	melibiose	-	builds acid from	from API rID32STR
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
116125	17632 ChEBI	nitrate	-	reduction
116125	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	-	builds acid from	from API rID32STR
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68381	27082 ChEBI	trehalose	-	builds acid from	from API rID32STR
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68381	15688 ChEBI	acetoin	+	from API rID32STR
116125	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
116125	alcohol dehydrogenase	+	1.1.1.1
68381	alkaline phosphatase	+	3.1.3.1	from API rID32STR
68381	alpha-galactosidase	-	3.2.1.22	from API rID32STR
68381	arginine dihydrolase	+	3.5.3.6	from API rID32STR
68381	beta-galactosidase	-	3.2.1.23	from API rID32STR
116125	beta-galactosidase	-	3.2.1.23
68381	beta-glucosidase	-	3.2.1.21	from API rID32STR
68381	beta-glucuronidase	+	3.2.1.31	from API rID32STR
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
6620	catalase	+	1.11.1.6
116125	catalase	-	1.11.1.6
6620	cytochrome-c oxidase	-	1.9.3.1
68381	glycyl tryptophan arylamidase	-		from API rID32STR
116125	lysine decarboxylase	-	4.1.1.18
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
116125	ornithine decarboxylase	-	4.1.1.17
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
116125	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	+	-	-	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
6620	+	-	-	+	-	+	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	+	-	-	+	-	-	-	-	-	-
6620	+	-	-	+	-	+	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	+	-	-	+/-	-	-	-	-	-	+
6620	+	-	-	+	-	+	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Fishes	-
#Host Body-Site	#Organ	#Brain

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Isolation date
6620	Fish, brain		Israel	ISR	Asia
67770	Brain of a fish (Tilapia)	Tilapia	Israel	ISR	Asia
116125	Other, Fish, brain		Israel	ISR	Asia	1988

Taxonmaps

69479

Global distribution of 16S sequence AB112407 (>99% sequence identity) for Streptococcus agalactiae subclade from Microbeatlas

Aquatic Samples	3036
Soil Samples	2237
Animal Samples	61262
Plant Samples	722
Total Samples	67257

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
6620			2	Risk group (German classification) According to TRBA 466
116125			2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM96744v1 assembly for Streptococcus agalactiae 2-22	complete	GCA_000967445	1261567.3	2619619105	1311	72.58

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptococcus difficilis gene for 16S rRNA, partial sequence	AB112407	1451	1311
20218	Streptococcus agalactiae strain ATCC 51487 16S ribosomal RNA genes, partial sequence	AH006153	475	1311
20218	Streptococcus difficile 16S ribosomal RNA gene, partial sequence; tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF064441	792	1311
124043	Streptococcus agalactiae strain ATCC 51487 16S ribosomal RNA gene, partial sequence.	KU561093	1438	1311

GC content

@ref	GC-content (mol%)	Method
67770	36.6-38	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptococcus agalactiae strain 22-Feb
PATRIC: 1261567.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.40	no
125439	gram_stain	BacteriaNetⓘ	positive	83.20	no
125439	motility	BacteriaNetⓘ	no	60.60	no
125439	spore_formation	BacteriaNetⓘ	no	74.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptococcus agalactiae strain 22-Feb
PATRIC: 1261567.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.52	no
125438	anaerobic	anaerobicⓘ	no	90.59	yes
125438	spore-forming	spore-formingⓘ	no	86.53	no
125438	aerobic	aerobicⓘ	no	94.84	yes
125438	thermophilic	thermophileⓘ	no	95.94	yes
125438	flagellated	motile2+ⓘ	no	88.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Multiplex PCR assay for detection of Streptococcus suis species and serotypes 2 and 1/2 in tonsils of live and dead pigs.	Marois C, Bougeard S, Gottschalk M, Kobisch M.	J Clin Microbiol	10.1128/jcm.42.7.3169-3175.2004	2004
	Development of a bacterial challenge test for gnotobiotic Nile tilapia Oreochromis niloticus larvae.	Situmorang ML, Dierckens K, Mlingi FT, Van Delsen B, Bossier P	Dis Aquat Organ	10.3354/dao02721	2014

References

#6620	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16828
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38467	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68381	Automatically annotated from API rID32STR .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116125	Collection of Institut Pasteur ; Curators of the CIP; CIP 103768
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptococcus difficilis (DSM 16828, ATCC 51487, CIP 103768)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 50CHac

API rID32STR

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000967445

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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