Name: Pullulanibacillus uraniitolerans UG-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14408

Type strain:

Species: Pullulanibacillus uraniitolerans

Strain Designation: UG-2

Culture col. no.: DSM 19429, LMG 24205

Strain history: <- M. da Costa, Univ. Coimbra, Portugal <- S. Pereira; UG-2

NCBI tax ID(s): 492809 (species)

SI-ID 324067

LMG 24205, DSM 19429

Section

Pullulanibacillus uraniitolerans UG-2 is an aerobe, spore-forming, mesophilic bacterium that was isolated from uranium mine effluent.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Sporolactobacillaceae*
	Genus	*Pullulanibacillus*
	Species	**Pullulanibacillus uraniitolerans**
	Full Scientific Name (LPSN)	Pullulanibacillus uraniitolerans Pereira et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30642]	Gram stain	positive

[Ref.: #30642]	Cell length	32 µm

[Ref.: #30642]	Cell width	0.5 µm

[Ref.: #30642]	Cell shape	rod-shaped

[Ref.: #30642]	Motility	no

[Ref.: #30642]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8010]

Culture medium

ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)

[Ref.: #8010]

Culture medium growth

[Ref.: #8010]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8010]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8010]	growth	35 °C
[Ref.: #30642]	growth	11-49 °C
[Ref.: #30642]	optimum	37 °C

[Ref.: #8010]	Temperature range	mesophilic
[Ref.: #30642]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30642]			optimum	4
[Ref.: #30642]			growth	03-06

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30642]

Oxygen tolerance

aerobe

[Ref.: #30642]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30642]		NaCl		growth	0-6	%
[Ref.: #30642]		NaCl		optimum	3	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30642]	arabinose ChEBI	+	carbon source
[Ref.: #30642]	asparagine ChEBI	+	carbon source
[Ref.: #30642]	cellobiose ChEBI	+	carbon source
[Ref.: #30642]	citrate ChEBI	+	carbon source
[Ref.: #30642]	esculin ChEBI	+	hydrolysis
[Ref.: #30642]	fructose ChEBI	+	carbon source
[Ref.: #30642]	galactose ChEBI	+	carbon source
[Ref.: #30642]	gluconate ChEBI	+	carbon source
[Ref.: #30642]	glucose ChEBI	+	carbon source
[Ref.: #30642]	glutamate ChEBI	+	carbon source
[Ref.: #30642]	glycerol ChEBI	+	carbon source
[Ref.: #30642]	lactose ChEBI	+	carbon source
[Ref.: #30642]	leucine ChEBI	+	carbon source
[Ref.: #30642]	maltose ChEBI	+	carbon source
[Ref.: #30642]	mannose ChEBI	+	carbon source
[Ref.: #30642]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30642]	nitrate ChEBI	+	reduction
[Ref.: #30642]	ornithine ChEBI	+	carbon source
[Ref.: #30642]	proline ChEBI	+	carbon source
[Ref.: #30642]	ribose ChEBI	+	carbon source
[Ref.: #30642]	sucrose ChEBI	+	carbon source
[Ref.: #30642]	trehalose ChEBI	+	carbon source
[Ref.: #30642]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30642]	acid phosphatase	+	3.1.3.2
[Ref.: #30642]	catalase	+	1.11.1.6
[Ref.: #30642]	gelatinase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8010]	Sample type/isolated from	uranium mine effluent
[Ref.: #8010]	Geographic location (country and/or sea, region)	Urgeiriça
[Ref.: #8010]	Country	Portugal
[Ref.: #8010]	Country ISO 3 Code	PRT
[Ref.: #8010]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM931441 (>99% sequence identity) for Pullulanibacillus uraniitolerans from Microbeatlas

Aquatic Samples	18
Soil Samples	150
Animal Samples	4
Plant Samples	4
Total Samples	176

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8010]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8010]	Pullulanibacillus uraniitolerans partial 16S rRNA gene, type strain UG-2T	AM931441	1530	ENA	492809 tax ID

[Ref.: #8010]

GC-content

39.5 mol%

Availability in culture collections External links

[Ref.: #8010]

Culture collection no.

DSM 19429, LMG 24205

[Ref.: #83552]

SI-ID 324067

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Scopulibacillus daqui sp. nov., a thermophilic bacterium isolated from high temperature daqu.	Yao S, Zhai L, Xin C, Liu Y, Xu L, Zhang X, Zhao T, Zhang L, Cheng C	Int J Syst Evol Microbiol	10.1099/ijsem.0.001417	2016	*
Phylogeny	Pullulanibacillus pueri sp. nov., isolated from Pu'er tea.	Niu L, Tang T, Song L, Xiong M, Tian J, Zhang K, Hu X, Zhu D	Int J Syst Evol Microbiol	10.1099/ijs.0.000239	2015	*
Phylogeny	Pullulanibacillus uraniitolerans sp. nov., an acidophilic, U(VI)-resistant species isolated from an acid uranium mill tailing effluent and emended description of the genus Pullulanibacillus.	Pereira SG, Albuquerque L, Nobre MF, Tiago I, Verissimo A, Pereira A, da Costa MS	Int J Syst Evol Microbiol	10.1099/ijs.0.040923-0	2012	*

References

#8010

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19429

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30642

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26973 (see below)

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#83552

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID324067.1 )

#26973

IJSEM 158 2013 ( DOI 10.1099/ijs.0.040923-0 , PubMed 22368171 )

* These data were automatically processed and therefore are not curated

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