Treponema brennaborense (DSM 12168, DD5/3)

Cell morphology

Multimedia

• @ref:	66793
  multimedia content:	EM_DSM_12168_1.jpg
  multimedia.multimedia content:	EM_DSM_12168_1.jpg
  caption:	electron microscopic image
  intellectual property rights:	© HZI/Manfred Rohde
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
4631	OMIZ-PAT (DSMZ Medium 1494)		Medium recipe at MediaDive	Name: . OMIZ-PAT Medium (modified) (DSMZ Medium 1494) Composition: (NH4)6Mo7O24 x 4 H2O 12.4 g/l Yeast extract 3.0 g/l Fe SO4 x 7 H2O 2.78015 g/l D-Glucose 2.0 g/l D-Mannose 2.0 g/l D-Arabinose 2.0 g/l L-Fucose 2.0 g/l D-Trehalose 2.0 g/l D-Sucrose 2.0 g/l L-Rhamnose 2.0 g/l NH4Cl 1.6 g/l Glutathione 1.5366 g/l Ascorbic acid 1.0 g/l NaHCO3 1.0 g/l Ethanol 1.0 g/l KCl 0.968 g/l D-Maltose 0.8 g/l D-glucuronic acid 0.8 g/l D-Mannitol 0.8 g/l D-galacturonic acid 0.8 g/l D-Fructose 0.8 g/l L-Glutamine 0.68 g/l L-Histidine 0.62 g/l NaVO3 0.61 g/l D 0.56 g/l Pyruvic acid 0.55 g/l L-Serine 0.525 g/l Fumaric acid 0.5 g/l HCl 0.3646 g/l L-Cysteine HCl 0.35 g/l L-Glutamic acid 0.3 g/l Formic acid 0.3 g/l ZnSO4 x 7 H2O 0.287 g/l MgSO4 x 7 H2O 0.25 g/l DL-carnitine 0.2 g/l N-Acetylglucosamine 0.2 g/l Citric acid 0.2 g/l L-Lysine HCl 0.182 g/l L-Arginine 0.175 g/l L-Ornithine HCl 0.17 g/l L-Phenylalanine 0.165 g/l CaCl2 x H2O 0.15 g/l L-Methionine 0.15 g/l L-Asparagine 0.15 g/l NaH2PO4 x H2O 0.14 g/l L-Leucine 0.131 g/l L-Isoleucine 0.131 g/l NiSO4 x 6 H2O 0.131 g/l L-Threonine 0.12 g/l L-Aspartic acid 0.118 g/l SnCl2 x 2 H2O 0.118 g/l L-Valine 0.117 g/l L-Proline 0.115 g/l L-Tryptophan 0.102 g/l Nicotinic acid 0.1 g/l Folinic acid 0.1 g/l L-Tyrosine 0.09 g/l NaOH 0.079994 g/l Glycine 0.075 g/l Nicotinamide 0.05 g/l Folic acid 0.05 g/l D-(+)-biotin 0.05 g/l Vitamine B12 0.05 g/l Choline chloride 0.05 g/l Myo-inositol 0.05 g/l L-Alanine 0.045 g/l Hemin 0.026076 g/l N-acetylmuramic acid 0.025 g/l Na2SeO3 0.0173 g/l MnSO4 x H2O 0.0169 g/l Riboflavine 0.01 g/l 2-Mercaptoethanesulfonic acid 0.01 g/l Phenol red 0.01 g/l Putrescine 2 HCl 0.005 g/l Pyridoxal hydrochloride 0.005 g/l Pyridoxal phosphate 0.005 g/l Calcium D-(+)-pantothenate 0.005 g/l Thiamine HCl 0.005 g/l Thiamin pyrophosphate 0.005 g/l Thymidine 0.0024 g/l N-(2-acetamido)-2-aminoethanesulfonic acid 0.0018 g/l Hypoxanthine 0.0014 g/l Uracil 0.0011 g/l Flavin adenine dinucleotide 0.001 g/l ß-nicotinamide adenine dinucleotide 0.001 g/l Coenzym A 0.001 g/l CuSO4 0.000789 g/l Cholesterol Asialofetuin 2-Mercaptoethanol Isovaleric acid Valeric acid 2-Methylbutyric acid Isobutyric acid DL-alpha-lipoic acid Rabbit serum Distilled water
4631	CHOPPED MEAT MEDIUM FOR TREPONEMA SP. (DSMZ Medium 78a)		Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM FOR TREPONEMA SP. (DSMZ Medium 78a) Composition: Ground beef 473.934 g/l Casitone 28.436 g/l Agar 14.218 g/l Yeast extract 4.73934 g/l K2HPO4 4.73934 g/l L-Glutamine 0.663507 g/l L-Histidine 0.56872 g/l L-Cysteine HCl 0.473934 g/l L-Serine 0.473934 g/l Ethanol 0.180095 g/l Vitamin K3 0.0473934 g/l Hemin 0.00473934 g/l Resazurin 0.000947867 g/l p-Aminobenzoic acid 0.000236967 g/l Vitamin B12 0.000236967 g/l Pantothenic acid 0.000236967 g/l Riboflavin 0.000236967 g/l alpha-lipoic acid 0.000236967 g/l Thiamine-HCl x 2 H2O 0.000236967 g/l Nicotine amide 0.000118483 g/l Nicotinic acid 0.000118483 g/l Biotin 9.47867e-05 g/l Folic acid 9.47867e-05 g/l Pyridoxamine hydrochloride 4.73934e-05 g/l NaOH Vitamin K1 Distilled water

Culture temp

Oxygen tolerance

Spore formation

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	aspartate and asparagine metabolism	100	9 of 9
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	threonine metabolism	100	10 of 10
66794	folate polyglutamylation	100	1 of 1
66794	ethanol fermentation	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	L-lactaldehyde degradation	100	3 of 3
66794	sulfopterin metabolism	100	4 of 4
66794	starch degradation	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	glycolysis	82.35	14 of 17
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycine betaine biosynthesis	80	4 of 5
66794	hydrogen production	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	valine metabolism	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	pyrimidine metabolism	71.11	32 of 45
66794	phenylalanine metabolism	69.23	9 of 13
66794	purine metabolism	69.15	65 of 94
66794	non-pathway related	68.42	26 of 38
66794	formaldehyde oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	vitamin B6 metabolism	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	methionine metabolism	61.54	16 of 26
66794	oxidative phosphorylation	61.54	56 of 91
66794	NAD metabolism	61.11	11 of 18
66794	glutamate and glutamine metabolism	60.71	17 of 28
66794	Entner Doudoroff pathway	60	6 of 10
66794	cellulose degradation	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	citric acid cycle	57.14	8 of 14
66794	degradation of hexoses	55.56	10 of 18
66794	cysteine metabolism	55.56	10 of 18
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	tryptophan metabolism	55.26	21 of 38
66794	metabolism of disaccharids	54.55	6 of 11
66794	vitamin B1 metabolism	53.85	7 of 13
66794	urea cycle	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	alanine metabolism	51.72	15 of 29
66794	coenzyme A metabolism	50	2 of 4
66794	adipate degradation	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	quinate degradation	50	1 of 2
66794	isoprenoid biosynthesis	50	13 of 26
66794	coenzyme M biosynthesis	50	5 of 10
66794	arginine metabolism	50	12 of 24
66794	toluene degradation	50	2 of 4
66794	ketogluconate metabolism	50	4 of 8
66794	butanoate fermentation	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	degradation of sugar acids	48	12 of 25
66794	degradation of pentoses	46.43	13 of 28
66794	leucine metabolism	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	lysine metabolism	45.24	19 of 42
66794	lipid A biosynthesis	44.44	4 of 9
66794	propanol degradation	42.86	3 of 7
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	O-antigen biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	methylglyoxal degradation	40	2 of 5
66794	d-xylose degradation	36.36	4 of 11
66794	glutathione metabolism	35.71	5 of 14
66794	tyrosine metabolism	35.71	5 of 14
66794	lipid metabolism	35.48	11 of 31
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	flavin biosynthesis	33.33	5 of 15
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	sulfate reduction	30.77	4 of 13
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	pentose phosphate pathway	27.27	3 of 11
66794	biotin biosynthesis	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	ascorbate metabolism	22.73	5 of 22
66794	molybdenum cofactor biosynthesis	22.22	2 of 9
66794	chlorophyll metabolism	22.22	4 of 18

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM21241v1 assembly for Treponema brennaborense DSM 12168	complete	GCA_000212415	906968.11	650716100	906968	99.23
66792	Treponema brennaborense DSM 12168	complete		906968.3		906968	62.1

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4631	Treponema brennaborense 16S rRNA gene	Y16568	1497		81028

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	The Presence of Treponema spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions.	Marcekova P, Mad'ar M, Stykova E, Kacirova J, Sondorova M, Mudron P, Zert Z.	Microorganisms	10.3390/microorganisms9112190	2021
Genetics	Variability of Inverted Repeats in All Available Genomes of Bacteria.	Porubiakova O, Havlik J, Indu, Sedy M, Prepechalova V, Bartas M, Bidula S, Stastny J, Fojta M, Brazda V.	Microbiol Spectr	10.1128/spectrum.01648-23	2023
	Phylogenetic analysis of pbp genes in treponemes.	Chadha T, Trindade AA.	Infect Ecol Epidemiol	10.3402/iee.v3i0.18636	2013
Metabolism	Molecular Evolution of the Oxygen-Binding Hemerythrin Domain.	Alvarez-Carreno C, Becerra A, Lazcano A.	PLoS One	10.1371/journal.pone.0157904	2016
	Promoter propagation in prokaryotes.	Matus-Garcia M, Nijveen H, van Passel MW.	Nucleic Acids Res	10.1093/nar/gks787	2012
Metabolism	Structural, bioinformatic, and in vivo analyses of two Treponema pallidum lipoproteins reveal a unique TRAP transporter.	Deka RK, Brautigam CA, Goldberg M, Schuck P, Tomchick DR, Norgard MV.	J Mol Biol	10.1016/j.jmb.2012.01.015	2012
Phylogeny	Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis.	Schrank K, Choi BK, Grund S, Moter A, Heuner K, Nattermann H, Gobel UB	Int J Syst Bacteriol	10.1099/00207713-49-1-43	1999