Geobacillus thermoglucosidasius (DSM 2542, ATCC 43742, NCIB 11955)

Name: Geobacillus thermoglucosidasius (DSM 2542, ATCC 43742, NCIB 11955)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1430

Type strain

Strain Designation KP 1006, R-35637, NRS-81

Culture col. no. DSM 2542 ATCC 43742 NCIB 11955 CCUG 28887 NRRL B-14233 6 more

NCBI tax ID(s) 1426

Special Collections DSMZ CCUG CIP Literature strains

History <- Y. Suzuki, KP 1006 (Bacillus thermoglucosidius) CIP <- 2001, CCUG <- 1991, L.K. Nakamura, NRRL, Peoria, USA: strain NRS-81

Links

Geobacillus thermoglucosidasius KP 1006 is a facultative anaerobe, spore-forming, Gram-positive prokaryote that forms circular colonies and was isolated from soil.

spore-forming Gram-positive motile rod-shaped colony-forming facultative anaerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 12264

LMG 17914,ATCC 43742,CCM 3731,DSM 2542,LMG 7137,NCIMB 11955,NBRC 107763,CCUG 28887,NRRL B-14233,CIP 106930,NCIB 11955,CECT 4038,CCRC 14687,KCTC 1828,KCTC 3400,BCRC 14687,NRRL B-14516,CECT 7250

@ref 20215

Last LPSN update 2025-12-16 09:47:06

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus thermoglucosidasius

Full scientific name Geobacillus thermoglucosidasius (Suzuki 1984) Nazina et al. 2001

Synonyms (4)

Geobacillus thermoglucosidans
Bacillus thermoglucosidasius
Parageobacillus thermoglucosidasius
"Parageobacillus thermoglucosidasius"

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
23042	positive	3.0-7.0 µm	0.5-1.2 µm	rod-shaped		peritrichous
123506	positive			rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
23042	0.5-5.0 mm	faintly brown	circular	1-2 days	trypticase soy agar

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
23042	trypticase soy agar
1116	BACILLUS THERMOGLUCOSIDASIUS MEDIUM (DSMZ Medium 305)	Medium recipe at MediaDive	Name: BACILLUS THERMOGLUCOSIDASIUS MEDIUM (DSMZ Medium 305) Composition: Agar 30.0 g/l Starch 10.0 g/l Peptone 5.0 g/l KH2PO4 3.0 g/l Yeast extract 3.0 g/l Meat extract 3.0 g/l Distilled water
40602	MEDIUM 370 - for Bacillus thermoglucosidasius		Distilled water make up to (1000.000 ml);Agar (30.000 g);Yeast extract (3.000 g);Peptone (5.000 g);Potassium di-hydrogen phosphate (3.000 g);Starch maize (10.000g);Beef extract (3.000 g)
1116	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
123506	CIP Medium 370	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1116	positive	growth	55	thermophilic
23042	negative	growth	37.0
23042	negative	growth	60.0
23042		maximum	<60.0	thermophilic
23042		minimum	40.0	thermophilic
23042	positive	optimum	50.0	thermophilic
40602	positive	growth	55	thermophilic
49444	positive	growth	56	thermophilic
123506	positive	growth	45-55	thermophilic
123506	negative	growth	10
123506	negative	growth	22
123506	negative	growth	30
123506	negative	growth	37

Culture pH

@ref	Ability	Type	PH
23042	positive	optimum	8.0
23042	positive	growth	7.0
23042		growth	9.0
23042		growth	6.0
23042		growth	5.0
123506		growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
23042	facultative anaerobe
49444	aerobe
123506	facultative anaerobe

Spore formation

@ref	Spore description	Type of spore	Confidence
23042	short ellipsoidal,subterminal and terminal,in swollen sporangia	endospore
123506
125438			91.582

Compound production

1116

Compoundexo-oligo-1,6 glucosidase

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23042	NaCl	positive	growth	<0.5 %
23042	NaCl		growth	1.0 %(w/v)
23042	NaCl		growth	5.0 %(w/v)
123506	NaCl	positive	growth	0 %
123506	NaCl		growth	2 %
123506	NaCl		growth	4 %
123506	NaCl		growth	6 %
123506	NaCl		growth	8 %
123506	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23042	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
23042	17426 ChEBI	5-dehydro-D-gluconate	-	builds acid from
23042	27613 ChEBI	amygdalin	-	builds gas from
23042	27613 ChEBI	amygdalin	+	builds acid from
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
23042	18305 ChEBI	arbutin	-	builds gas from
23042	18305 ChEBI	arbutin	+	builds acid from
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
23042		casein	-	hydrolysis
23042	17057 ChEBI	cellobiose	-	builds gas from
23042	17057 ChEBI	cellobiose	+	builds acid from
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
23042	16947 ChEBI	citrate	-	carbon source
23042	17108 ChEBI	D-arabinose	-	builds acid from
68371	17108 ChEBI	D-arabinose	+	builds acid from	from API 50CH acid
23042	18333 ChEBI	D-arabitol	-	builds acid from
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
23042	15824 ChEBI	D-fructose	-	builds gas from
23042	15824 ChEBI	D-fructose	+	builds acid from
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
23042	28847 ChEBI	D-fucose	-	builds acid from
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
23042	17634 ChEBI	D-glucose	-	builds gas from
23042	17634 ChEBI	D-glucose	+	builds acid from
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
23042	62318 ChEBI	D-lyxose	-	builds acid from
68371	62318 ChEBI	D-lyxose	+	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
23042	16024 ChEBI	D-mannose	-	builds gas from
23042	16024 ChEBI	D-mannose	+	builds acid from
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
23042	16443 ChEBI	D-tagatose	-	builds acid from
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
23042	65327 ChEBI	D-xylose	-	builds gas from
23042	65327 ChEBI	D-xylose	+	builds acid from
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
23042	17113 ChEBI	erythritol	-	builds acid from
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
23042	4853 ChEBI	esculin	+	hydrolysis
123506	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
23042	16813 ChEBI	galactitol	-	builds acid from
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
23042	28260 ChEBI	galactose	-	builds acid from
23042	5291 ChEBI	gelatin	+	hydrolysis
23042	28066 ChEBI	gentiobiose	-	builds gas from
23042	28066 ChEBI	gentiobiose	+	builds acid from
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
23042	24265 ChEBI	gluconate	-	builds acid from
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
23042	17754 ChEBI	glycerol	-	builds gas from
23042	17754 ChEBI	glycerol	+	builds acid from
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
23042	28087 ChEBI	glycogen	-	builds acid from
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
123506	606565 ChEBI	hippurate	-	hydrolysis
23042	15443 ChEBI	inulin	-	builds acid from
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
23042	30849 ChEBI	L-arabinose	-	builds gas from
23042	30849 ChEBI	L-arabinose	+	builds acid from
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
23042	18403 ChEBI	L-arabitol	-	builds acid from
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
23042	18287 ChEBI	L-fucose	-	builds acid from
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
23042	17266 ChEBI	L-sorbose	-	builds gas from
23042	17266 ChEBI	L-sorbose	+	builds acid from
68371	17266 ChEBI	L-sorbose	+	builds acid from	from API 50CH acid
23042	65328 ChEBI	L-xylose	-	builds acid from
68371	65328 ChEBI	L-xylose	+	builds acid from	from API 50CH acid
23042	17716 ChEBI	lactose	-	builds gas from
23042	17716 ChEBI	lactose	+	builds acid from
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
23042	17306 ChEBI	maltose	-	builds gas from
23042	17306 ChEBI	maltose	+	builds acid from
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
23042	29864 ChEBI	mannitol	-	builds gas from
23042	29864 ChEBI	mannitol	+	builds acid from
23042	6731 ChEBI	melezitose	-	builds acid from
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
23042	28053 ChEBI	melibiose	-	builds acid from
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
23042	37657 ChEBI	methyl D-glucoside	-	builds gas from
23042	37657 ChEBI	methyl D-glucoside	+	builds acid from
23042	17268 ChEBI	myo-inositol	-	builds acid from
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
23042	506227 ChEBI	N-acetylglucosamine	-	builds gas from
23042	506227 ChEBI	N-acetylglucosamine	+	builds acid from
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
23042	17632 ChEBI	nitrate	-	reduction
123506	17632 ChEBI	nitrate	+	reduction
123506	17632 ChEBI	nitrate	+	respiration
123506	16301 ChEBI	nitrite	+	reduction
23042		O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
23042	16634 ChEBI	raffinose	-	builds acid from
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
23042	26546 ChEBI	rhamnose	-	builds gas from
23042	26546 ChEBI	rhamnose	+	builds acid from
23042	15963 ChEBI	ribitol	-	builds acid from
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
23042	33942 ChEBI	ribose	-	builds gas from
23042	33942 ChEBI	ribose	+	builds acid from
23042	17814 ChEBI	salicin	-	builds gas from
23042	17814 ChEBI	salicin	+	builds acid from
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
23042	30911 ChEBI	sorbitol	-	builds gas from
23042	30911 ChEBI	sorbitol	+	builds acid from
23042	28017 ChEBI	starch	-	builds gas from
23042	28017 ChEBI	starch	+	builds acid from
23042	28017 ChEBI	starch	+	hydrolysis
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
23042	17992 ChEBI	sucrose	-	builds gas from
23042	17992 ChEBI	sucrose	+	builds acid from
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
23042	27082 ChEBI	trehalose	-	builds gas from
23042	27082 ChEBI	trehalose	+	builds acid from
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
23042	27897 ChEBI	tryptophan	-	energy source
23042	32528 ChEBI	turanose	-	builds gas from
23042	32528 ChEBI	turanose	+	builds acid from
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
23042	17151 ChEBI	xylitol	-	builds acid from
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
23042	15688 ChEBI	acetoin
23042	16136 ChEBI	hydrogen sulfide
23042	35581 ChEBI	indole
123506	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Citrate test	Methylred-test	Indole test
23042	15688 ChEBI	acetoin	-
123506	15688 ChEBI	acetoin	-
23042	16947 ChEBI	citrate		-
123506	17234 ChEBI	glucose			+
23042	35581 ChEBI	indole				-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123506	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123506	amylase	-
23042	arginine dihydrolase	-	3.5.3.6
68382	beta-galactosidase	-	3.2.1.23	from API zym
123506	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123506	caseinase	+	3.4.21.50
23042	catalase	+	1.11.1.6
123506	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23042	cytochrome oxidase	+	1.9.3.1
123506	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123506	gamma-glutamyltransferase	-	2.3.2.2
123506	gelatinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
23042	lysine decarboxylase	-	4.1.1.18
123506	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
123506	ornithine decarboxylase	-	4.1.1.17
123506	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
23042	tryptophan deaminase	-	4.1.99.1
123506	tween esterase	-
23042	urease	-	3.5.1.5
123506	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123506	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123506	not determinedn.d.	-	-	+	+	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1116	soil	Kyoto, Shimogamo	Japan	JPN	Asia
49444	Soil
123506	Environment, Soil

Taxonmaps

69479

Global distribution of 16S sequence FN428685 (>99% sequence identity) for Bacillaceae from Microbeatlas

Aquatic Samples	2043
Soil Samples	4724
Animal Samples	5920
Plant Samples	1962
Total Samples	14649

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1116	1	Risk group (German classification) According to TRBA 466
123506	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM170098v1 assembly for Parageobacillus thermoglucosidasius NCIMB 11955	complete	GCA_001700985		2718218464	1426	98.28
66792	ASM129536v1 assembly for Parageobacillus thermoglucosidasius DSM 2542	complete	GCA_001295365	1426.100	2687453436	1426	96.4
66792	DSM2542-Assem1 assembly for Parageobacillus thermoglucosidasius DSM 2542	contig	GCA_000966225	1426.99	2627853848	1426	62.39

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Bacillus thermoglucosidasius gene for 16S ribosomal RNA	AB021197	1523	1426
20218	Geobacillus thermoglucosidasius strain DSM 2542 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157954	259	1426
20218	Geobacillus thermoglucosidasius isolate Gtg15 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723644	503	1426
20218	Geobacillus thermoglucosidasius isolate Gtg21 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723645	573	1426
20218	Geobacillus thermoglucosidasius isolate Gtg33 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723646	479	1426
20218	Bacillus thermoglucosidasius 16S ribosomal RNA	X60641	1435	1426
1116	Geobacillus thermoglucosidasius partial 16S rRNA gene, strain R-35637	FN428685	1511	1426

GC content

@ref	GC-content (mol%)	Method
1116	43.4	high performance liquid chromatography (HPLC)
1116	45.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Parageobacillus thermoglucosidasius DSM 2542
NCBI: GCA_001295365

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	89.10	no
125439	motility	BacteriaNetⓘ	yes	85.60	no
125439	gram_stain	BacteriaNetⓘ	variable	85.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	75.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Parageobacillus thermoglucosidasius DSM 2542
NCBI: GCA_001295365.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	74.59	yes
125438	anaerobic	anaerobicⓘ	no	84.08	no
125438	aerobic	aerobicⓘ	yes	54.85	no
125438	spore-forming	spore-formingⓘ	yes	91.58	yes
125438	thermophilic	thermophileⓘ	yes	65.43	yes
125438	flagellated	motile2+ⓘ	yes	81.26	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Characterization of germinant receptors of P. thermoglucosidasius NCIMB 11955.	Salvador M, Lau MSH, Minton NP, Condon S, Gayan E.	Curr Res Microb Sci	10.1016/j.crmicr.2025.100485	2025
	Metabolic engineering of Parageobacillus thermoglucosidasius for thermophilic production of 1-butanol.	Domenech P, Pogrebnyakov I, Jensen SI, Driessen JLSP, Riisager A, Nielsen AT.	AMB Express	10.1186/s13568-025-01879-5	2025
	Development of a xylose-inducible and glucose-insensitive expression system for Parageobacillus thermoglucosidasius.	Wang J, Wang W, Chen Y, Liu Z, Ji X, Pan G, Li Z, Fan K.	Appl Microbiol Biotechnol	10.1007/s00253-024-13333-w	2024
	Functional characterization of the phosphotransferase system in Parageobacillus thermoglucosidasius.	Bidart GN, Gharabli H, Welner DH.	Sci Rep	10.1038/s41598-023-33918-1	2023
	Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius.	Koniuchovaite A, Petkeviciute A, Bernotaite E, Gricajeva A, Gegeckas A, Kalediene L, Kaunietis A.	Front Microbiol	10.3389/fmicb.2023.1207367	2023
	Metabolic engineering for the production of acetoin and 2,3-butanediol at elevated temperature in Parageobacillus thermoglucosidasius NCIMB 11955.	Sheng L, Madika A, Lau MSH, Zhang Y, Minton NP.	Front Bioeng Biotechnol	10.3389/fbioe.2023.1191079	2023
	Efficient pathway-driven scyllo-inositol production from myo-inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control.	Kurashiki R, Takahashi M, Okumura Y, Ono T, Endo H, Makino K, Fukui K, Yokoyama K, Ishikawa S, Yoshida K-i, Ohshiro T, Suzuki H.	Appl Environ Microbiol	10.1128/aem.00281-24	2024
	Genomic identification of germinant receptors and phenotypic response to germinants in Parageobacillus and Geobacillus spp. strains.	Salvador M, Yruela I, Condon S, Gayan E.	Curr Res Microb Sci	10.1016/j.crmicr.2025.100461	2025
	Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666^T.	Lobastova T, Fokina V, Tarlachkov S, Shutov A, Bragin E, Kazantsev A, Donova M.	Microorganisms	10.3390/microorganisms9122554	2021
Enzymology	Diversity analysis of thermophilic hydrogenogenic carboxydotrophs by carbon monoxide dehydrogenase amplicon sequencing using new primers.	Omae K, Oguro T, Inoue M, Fukuyama Y, Yoshida T, Sako Y.	Extremophiles	10.1007/s00792-020-01211-y	2021
Biotechnology	In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria.	Frenzel E, Legebeke J, van Stralen A, van Kranenburg R, Kuipers OP.	Biotechnol Biofuels	10.1186/s13068-017-1008-5	2018
Metabolism	A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR.	Xiao D, Zhang W, Guo X, Liu Y, Hu C, Guo S, Kang Z, Xu X, Ma C, Gao C, Xu P.	Nat Commun	10.1038/s41467-021-27357-7	2021
Genetics	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.	Daas MJA, Vriesendorp B, van de Weijer AHP, van der Oost J, van Kranenburg R.	Curr Microbiol	10.1007/s00284-017-1349-0	2018
Genetics	The engineering toolbox of Parageobacillus thermoglucosidasius.	Millgaard M, Escher B, Pogrebnyakov I, Nielsen AT.	Appl Microbiol Biotechnol	10.1007/s00253-025-13508-z	2025
	Parageobacillus thermoglucosidasius Strain Engineering Using a Theophylline Responsive RiboCas for Controlled Gene Expression.	Lau MSH, Madika A, Zhang Y, Minton NP.	ACS Synth Biol	10.1021/acssynbio.3c00735	2024
Genetics	Elucidating the biotechnological potential of the genera Parageobacillus and Saccharococcus through comparative genomic and pan-genome analysis.	Mol M, de Maayer P.	BMC Genomics	10.1186/s12864-024-10635-1	2024
Enzymology	A highly efficient method for genomic deletion across diverse lengths in thermophilic Parageobacillus thermoglucosidasius.	Yang Z, Li B, Bu R, Wang Z, Xin Z, Li Z, Zhang L, Wang W.	Synth Syst Biotechnol	10.1016/j.synbio.2024.05.009	2024
	A thermostable type I-B CRISPR-Cas system for orthogonal and multiplexed genetic engineering.	Yang Z, Li Z, Li B, Bu R, Tan GY, Wang Z, Yan H, Xin Z, Zhang G, Li M, Xiang H, Zhang L, Wang W.	Nat Commun	10.1038/s41467-023-41973-5	2023
	Genome-scale metabolic modelling of extremophiles and its applications in astrobiological environments.	Noirungsee N, Changkhong S, Phinyo K, Suwannajak C, Tanakul N, Inwongwan S.	Environ Microbiol Rep	10.1111/1758-2229.13231	2024
Metabolism	Genome-scale metabolic modeling of P. thermoglucosidasius NCIMB 11955 reveals metabolic bottlenecks in anaerobic metabolism.	Mol V, Bennett M, Sanchez BJ, Lisowska BK, Herrgard MJ, Nielsen AT, Leak DJ, Sonnenschein N.	Metab Eng	10.1016/j.ymben.2021.03.002	2021
	Ethanologenic fermentation by Parageobacillus thermoglucosidasius with continuous hot microbubble gas-stripping	Calverley J, Ibenegbu C, Hussein-Sheik A, Hemaka Bandulasena H, Leak D.	Microb Cell Fact		2024
	Draft Genome Sequence of Parageobacillus thermoglucosidasius Strain TG4, a Hydrogenogenic Carboxydotrophic Bacterium Isolated from a Marine Sediment.	Inoue M, Tanimura A, Ogami Y, Hino T, Okunishi S, Maeda H, Yoshida T, Sako Y.	Microbiol Resour Announc	10.1128/mra.01666-18	2019
	Diversity and distribution of thermophilic hydrogenogenic carboxydotrophs revealed by microbial community analysis in sediments from multiple hydrothermal environments in Japan.	Omae K, Fukuyama Y, Yasuda H, Mise K, Yoshida T, Sako Y.	Arch Microbiol	10.1007/s00203-019-01661-9	2019
Genetics	Genome-scale reconstruction and metabolic modelling of the fast-growing thermophile Geobacillus sp. LC300.	Ljungqvist E, Gustavsson M.	Metab Eng Commun	10.1016/j.mec.2022.e00212	2022
	Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus thermoglucosidasius.	Wang J, Li Z, Wang W, Pang S, Yao Y, Yuan F, Wang H, Xu Z, Pan G, Liu Z, Chen Y, Fan K	ACS Synth Biol	10.1021/acssynbio.2c00087	2022
Metabolism	Relaxed control of sugar utilization in Parageobacillus thermoglucosidasius DSM 2542.	Liang J, Roberts A, van Kranenburg R, Bolhuis A, Leak DJ	Microbiol Res	10.1016/j.micres.2021.126957	2021
Metabolism	Metabolic engineering of Parageobacillus thermoglucosidasius for the efficient production of (2R, 3R)-butanediol.	Zhou J, Lian J, Rao CV	Appl Microbiol Biotechnol	10.1007/s00253-020-10553-8	2020
Enzymology	Biochemical characterization of a novel thermostable feruloyl esterase from Geobacillus thermoglucosidasius DSM 2542(T).	Ay Sal F, Colak DN, Guler HI, Canakci S, Belduz AO	Mol Biol Rep	10.1007/s11033-019-04893-6	2019
Metabolism	A design of experiments approach for the rapid formulation of a chemically defined medium for metabolic profiling of industrially important microbes.	Singleton C, Gilman J, Rollit J, Zhang K, Parker DA, Love J	PLoS One	10.1371/journal.pone.0218208	2019
Metabolism	Comparative genomic analysis of Parageobacillus thermoglucosidasius strains with distinct hydrogenogenic capacities.	Mohr T, Aliyu H, Kuchlin R, Zwick M, Cowan D, Neumann A, de Maayer P	BMC Genomics	10.1186/s12864-018-5302-9	2018
Metabolism	CO-dependent hydrogen production by the facultative anaerobe Parageobacillus thermoglucosidasius.	Mohr T, Aliyu H, Kuchlin R, Polliack S, Zwick M, Neumann A, Cowan D, de Maayer P	Microb Cell Fact	10.1186/s12934-018-0954-3	2018
Genetics	Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain.	Sheng L, Zhang Y, Minton NP	Genome Announc	10.1128/genomeA.01065-16	2016
Genetics	Genome sequence of Geobacillus thermoglucosidasius DSM2542, a platform hosts for biotechnological applications with industrial potential.	Chen J, Zhang Z, Zhang C, Yu B	J Biotechnol	10.1016/j.jbiotec.2015.10.002	2015
Phylogeny	Isolation and characterization of two novel ethanol-tolerant facultative-anaerobic thermophilic bacteria strains from waste compost.	Fong JC, Svenson CJ, Nakasugi K, Leong CT, Bowman JP, Chen B, Glenn DR, Neilan BA, Rogers PL	Extremophiles	10.1007/s00792-006-0507-2	2006
Enzymology	Proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006.	Watanabe K, Chishiro K, Kitamura K, Suzuki Y	J Biol Chem	S0021-9258(18)54226-5	1991
Enzymology	Primary structure of the oligo-1,6-glucosidase of Bacillus cereus ATCC7064 deduced from the nucleotide sequence of the cloned gene.	Watanabe K, Kitamura K, Iha H, Suzuki Y	Eur J Biochem	10.1111/j.1432-1033.1990.tb19267.x	1990
Enzymology	Purification and characterization of Bacillus coagulans oligo-1,6-glucosidase.	Suzuki Y, Tomura Y	Eur J Biochem	10.1111/j.1432-1033.1986.tb09723.x	1986
	Removing carbon catabolite repression in Parageobacillus thermoglucosidasius DSM 2542.	Liang J, van Kranenburg R, Bolhuis A, Leak DJ	Front Microbiol	10.3389/fmicb.2022.985465	2022
Phylogeny	Bacillus thermoglucosidasius sp. nov., a New Species of Obligately Thermophilic Bacilli.	Suzuki Y, Kishigami T, Inoue K, Mizoguchi Y, Eto N, Takagi M, Abe S	Syst Appl Microbiol	10.1016/S0723-2020(83)80006-X	1983

References

#1116	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2542
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23042	An Coorevits,Anna E. Dinsdale,Gillian Halket,Liesbeth Lebbe,Paul De Vos,Anita Van Landschoot,Niall A. Logan: Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.. IJSEM 62: 1470 - 1485 2012 ( DOI 10.1099/ijs.0.030346-0 , PubMed 21856988 )
#40602	; Curators of the CIP;
#49444	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28887
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123506	Collection of Institut Pasteur ; Curators of the CIP; CIP 106930
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Geobacillus thermoglucosidasius (DSM 2542, ATCC 43742, NCIB 11955)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001700985

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media