Name: Bacillus paranthracis Mn5
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140960

Type strain:

Species: Bacillus paranthracis

Strain Designation: Mn5

Culture col. no.: MCCC 1A00395, KCTC 33714, LMG 28873, NBRC 113914, CIP 111454

Strain history: CIP <- 2017, LMG <- 2015, Y. Liu, Third Inst. Ocean., Fujian Province, Xiamen, China: strain Mn5 <- M. Tian

NCBI tax ID(s): 2026186 (species)

Special Collections

Other strains from Bacillus paranthracis

Section

Bacillus paranthracis Mn5 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from sediment of the Pacific Ocean.

colony-forming
Gram-positive
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus paranthracis**
	Full Scientific Name (LPSN)	Bacillus paranthracis Liu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43860]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #121571]	Gram stain	positive

[Ref.: #43860]	Cell length	2.8-3.3 µm

[Ref.: #43860]	Cell width	1.2-1.4 µm

[Ref.: #43860]	Cell shape	rod-shaped
[Ref.: #121571]	Cell shape	rod-shaped

[Ref.: #43860]	Motility	no
[Ref.: #121571]	Motility	no

[Ref.: #43860]	Colony size	2-3 mm
[Ref.: #43860]	Colony color	off-white
[Ref.: #43860]	Colony shape	circular
[Ref.: #43860]	Incubation period	2 days
[Ref.: #43860]	Cultivation medium used	Luria Bertani agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43860]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43860]

Culture medium growth

[Ref.: #43860]

Culture medium

Trypticase soy agar

[Ref.: #43860]

Culture medium growth

[Ref.: #121571]

Culture medium

CIP Medium 72

[Ref.: #121571]

Culture medium growth

[Ref.: #121571]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #43860]	growth	15-45 °C
[Ref.: #43860]	optimum	30 °C
[Ref.: #67771]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #43860]			optimum	7.0-8.0
[Ref.: #43860]			growth	5.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43860]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43860]	Spore description	central elliptical endospore
[Ref.: #43860]	Type of spore	endospore
[Ref.: #43860]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43860]		NaCl		growth	0-9	%(w/v)
[Ref.: #43860]		NaCl		optimum	1-2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43860]	amygdalin ChEBI	-	builds acid from
[Ref.: #43860]	arabinose ChEBI	-	builds acid from
[Ref.: #43860]	casein	+	hydrolysis
[Ref.: #43860]	citrate ChEBI	+	carbon source
[Ref.: #43860]	D-ribose ChEBI	+	builds acid from
[Ref.: #43860]	glucose ChEBI	-	builds acid from
[Ref.: #43860]	glycerol ChEBI	-	builds acid from
[Ref.: #43860]	mannitol ChEBI	-	builds acid from
[Ref.: #43860]	melibiose ChEBI	-	builds acid from
[Ref.: #43860]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43860]	rhamnose ChEBI	-	builds acid from
[Ref.: #43860]	skimmed milk	+	hydrolysis
[Ref.: #43860]	sorbitol ChEBI	-	builds acid from
[Ref.: #43860]	starch ChEBI	-	hydrolysis
[Ref.: #43860]	sucrose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43860]	acetoin ChEBI	yes
[Ref.: #43860]	hydrogen sulfide ChEBI	no
[Ref.: #43860]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43860]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #43860]	arginine dihydrolase	+	3.5.3.6
[Ref.: #43860]	beta-galactosidase	-	3.2.1.23
[Ref.: #43860]	catalase	+	1.11.1.6
[Ref.: #43860]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43860]	gelatinase	+
[Ref.: #43860]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43860]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43860]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43860]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43860]

Fatty acid	Percentage
C15:0 iso	14.7
C16:0	14.6
C16:1ω7c / C15:0 iso 2OH	9
C16:0 iso	7.4
C13:0 iso	7
C17:0 iso	5.6
C15:0 anteiso	5.6
C14:0 iso	5.5
C14:0	5.4
C17:1 iso I /C17:1 anteiso B	3.8
C18:1ω9c	2.4
C17:0 anteiso	2.2
C17:1 iso ω5c	2.1
C18:0	1.8
C14:0 3OH / C16:1 iso I	1.7
C17:1 iso ω10c	1.7
C13:0 anteiso	1.7
C12:0	1.6
C12:0 iso	1.4
C16:1ω11c	1
C17:1 anteiso A	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43860]	Sample type/isolated from	sediment of the Pacific Ocean

[Ref.: #67771]	Sample type/isolated from	From sediments
[Ref.: #67771]	Geographic location (country and/or sea, region)	the Pacific Ocean
[Ref.: #67771]	Country	Pacific Ocean

[Ref.: #121571]	Sample type/isolated from	Environment, Sediments
[Ref.: #121571]	Country	Pacific Ocean
[Ref.: #121571]	Isolation date	2005-01-03
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ812420 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	446
Soil Samples	1920
Animal Samples	1524
Plant Samples	1079
Total Samples	4969

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #121571]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43860]	Bacillus paranthracis strain MCCC 1A00395 16S ribosomal RNA gene, partial sequence	KJ812420		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus paranthracis Mn5	GCA_001883995	contig	GenBank ENA	2026186 tax ID	*

[Ref.: #43860]

GC-content

35.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus paranthracis Mn5 NCBI: GCA_001883995.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	73.73	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.31	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	51.778	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	85.486	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.329	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.765	no

Availability in culture collections External links

[Ref.: #43860]

Culture collection no.

MCCC 1A00395, KCTC 33714, LMG 28873, NBRC 113914, CIP 111454

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of nine novel species of the Bacillus cereus group.	Liu Y, Du J, Lai Q, Zeng R, Ye D, Xu J, Shao Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.001821	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43860

Yang Liu, Juan Du, Qiliang Lai, Runying Zeng, Dezan Ye, Jun Xu, Zongze Shao: Proposal of nine novel species of the Bacillus cereus group. IJSEM 67: 2499 - 2508 2017 ( DOI 10.1099/ijsem.0.001821 , PubMed 28792367 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#121571 Collection of Institut Pasteur ; Curators of the CIP; CIP 111454
* These data were automatically processed and therefore are not curated

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