Name: Paraclostridium benzoelyticum JC272
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140901

Type strain:

Species: Paraclostridium benzoelyticum

Strain Designation: JC272

Culture col. no.: KCTC 15476, LMG 28745

Strain history: <- Ch Sasikala, JNT Univ, India

NCBI tax ID(s): 1629550 (species)

Section

Paraclostridium benzoelyticum JC272 is an obligate anaerobe, chemoorganoheterotroph, spore-forming bacterium that was isolated from Sediment and soil samples from different marine habitats of Gujarat.

Gram-positive
motile
rod-shaped
spore-forming
chemoorganoheterotroph
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Paraclostridium*
	Species	**Paraclostridium benzoelyticum**
	Full Scientific Name (LPSN)	Paraclostridium benzoelyticum Sasi Jyothsna et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43740]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #43740]	Cell length	2-4 µm

[Ref.: #43740]	Cell width	0.5-0.9 µm

[Ref.: #43740]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43740]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #43740]	growth	25-40 °C
[Ref.: #43740]	optimum	30-35 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #43740]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43740]			optimum	7-8
[Ref.: #43740]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43740]	Oxygen tolerance	obligate anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #43740]

Nutrition type

chemoorganoheterotroph

[Ref.: #43740]	Spore description	terminal
[Ref.: #43740]	Type of spore	endospore
[Ref.: #43740]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43740]		NaCl		growth	0-2	%(w/v)
[Ref.: #43740]		NaCl		optimum	0-1	%(w/v)

[Ref.: #43740]

Observation

Antimicrobials are produced

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43740]	ascorbate ChEBI	+	assimilation
[Ref.: #43740]	benzoate ChEBI	+	growth
[Ref.: #43740]	butyrate ChEBI	+	assimilation
[Ref.: #43740]	casein	+	hydrolysis
[Ref.: #43740]	cellobiose ChEBI	+	growth
[Ref.: #43740]	cellulose ChEBI	+	growth
[Ref.: #43740]	citrate ChEBI	-	assimilation
[Ref.: #43740]	D-fructose ChEBI	+	assimilation
[Ref.: #43740]	D-glucose ChEBI	+	assimilation
[Ref.: #43740]	D-mannitol ChEBI	+	growth
[Ref.: #43740]	D-mannose ChEBI	+	growth
[Ref.: #43740]	D-ribose ChEBI	-	assimilation
[Ref.: #43740]	D-sorbitol ChEBI	+	growth
[Ref.: #43740]	ethanol ChEBI	-	growth
[Ref.: #43740]	fumarate ChEBI	+	assimilation
[Ref.: #43740]	fumarate ChEBI	+	growth
[Ref.: #43740]	gelatin ChEBI	+	hydrolysis
[Ref.: #43740]	glucose ChEBI	-	fermentation
[Ref.: #43740]	glycerol ChEBI	+	assimilation
[Ref.: #43740]	glycolate ChEBI	+	assimilation
[Ref.: #43740]	lactate ChEBI	-	growth
[Ref.: #43740]	lactose ChEBI	-	assimilation
[Ref.: #43740]	lactose ChEBI	-	growth
[Ref.: #43740]	malate ChEBI	+	assimilation
[Ref.: #43740]	nitrate ChEBI	-	reduction
[Ref.: #43740]	ribose ChEBI	-	growth
[Ref.: #43740]	starch ChEBI	+	growth
[Ref.: #43740]	starch ChEBI	+	hydrolysis
[Ref.: #43740]	succinate ChEBI	-	assimilation
[Ref.: #43740]	succinate ChEBI	-	growth
[Ref.: #43740]	sucrose ChEBI	-	assimilation
[Ref.: #43740]	sucrose ChEBI	-	growth
[Ref.: #43740]	tryptophan ChEBI	-	energy source
[Ref.: #43740]	yeast extract	+	required for growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43740]	ampicillin ChEBI	yes	100	µg/mL
[Ref.: #43740]	chloramphenicol ChEBI	yes	50	µg/mL
[Ref.: #43740]	erythromycin ChEBI	yes	100	µg/mL
[Ref.: #43740]	fosfomycin ChEBI				yes	100
[Ref.: #43740]	gentamicin ChEBI				yes	50
[Ref.: #43740]	kanamycin ChEBI				yes	100	µg/mL
[Ref.: #43740]	metronidazole ChEBI	yes	100	µg/mL
[Ref.: #43740]	nalidixic acid ChEBI				yes	100
[Ref.: #43740]	penicillin ChEBI	yes	50	µg/mL
[Ref.: #43740]	polymyxin b ChEBI				yes	100
[Ref.: #43740]	rifampicin ChEBI				yes	100
[Ref.: #43740]	tetracycline ChEBI				yes	100
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43740]	acetoin ChEBI	no
[Ref.: #43740]	hydrogen sulfide ChEBI	yes
[Ref.: #43740]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #43740]	acetoin ChEBI	-
[Ref.: #43740]	glucose ChEBI		-
[Ref.: #43740]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #43740]	catalase	-	1.11.1.6
[Ref.: #43740]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43740]	lipase (C 14)	+
[Ref.: #43740]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43740]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43740]	Sample type/isolated from	Sediment and soil samples from different marine habitats of Gujarat
[Ref.: #43740]	Geographic location (country and/or sea, region)	Gujarat
[Ref.: #43740]	Country	India
[Ref.: #43740]	Country ISO 3 Code	IND
[Ref.: #43740]	Continent	Asia
[Ref.: #43740]	Isolation date	2013-12

[Ref.: #67771]	Sample type/isolated from	From marine water with sediment
[Ref.: #67771]	Geographic location (country and/or sea, region)	Rann of Kutch
[Ref.: #67771]	Country	India
[Ref.: #67771]	Country ISO 3 Code	IND
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN846800 (>99% sequence identity) for Paeniclostridium from Microbeatlas

Aquatic Samples	9424
Soil Samples	14269
Animal Samples	25127
Plant Samples	2734
Total Samples	51554

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43740]	16S rRNA gene sequence	LN846800		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67771]	Paraclostridium benzoelyticum JC272	GCA_001006285	contig	GenBank	1629550 tax ID
[Ref.: #66792]	Paraclostridium benzoelyticum JC272	2832979616	complete	JGI IMG/M	1629550 tax ID	*
[Ref.: #66792]	Paraclostridium benzoelyticum JC272	2767802767	complete	JGI IMG/M	1629550 tax ID	*

[Ref.: #43740]	GC-content	28.3 mol%	genome sequence analysis
[Ref.: #43740]	GC-content	29.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	28.3 mol%

Availability in culture collections External links

[Ref.: #43740]

Culture collection no.

KCTC 15476, LMG 28745

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paraclostridium benzoelyticum gen. nov., sp. nov., isolated from marine sediment and reclassification of Clostridium bifermentans as Paraclostridium bifermentans comb. nov. Proposal of a new genus Paeniclostridium gen. nov. to accommodate Clostridium sordellii and Clostridium ghonii.	Sasi Jyothsna TS, Tushar L, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijsem.0.000874	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43740

T. S. Sasi Jyothsna, L. Tushar, Ch. Sasikala, Ch. V. Ramana: Paraclostridium benzoelyticum gen. nov., sp. nov., isolated from marine sediment and reclassification of Clostridium bifermentans as Paraclostridium bifermentans comb. nov. Proposal of a new genus Paeniclostridium gen. nov. to accommodate Clostridium sordellii and Clostridium ghonii. IJSEM 66: 1268 - 1274 2016 ( DOI 10.1099/ijsem.0.000874 , PubMed 26738915 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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