Halanaeroarchaeum sulfurireducens (JCM 30661, UNIQEM U935, HSR2)

Name: Halanaeroarchaeum sulfurireducens (JCM 30661, UNIQEM U935, HSR2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140834

Type strain

Strain Designation HSR2

Culture col. no. JCM 30661 UNIQEM U935 HSR2

NCBI tax ID(s) 1604004

Special Collections JCM Literature strains

History JCM <-- D. Y. Sorokin; Delft Univ. of Technol., Netherlands; HSR2.

Links

Halanaeroarchaeum sulfurireducens HSR2 is an obligate anaerobe, Gram-negative, coccus-shaped archaeon that was isolated from anaerobic sediments of chloride-sulfate hypersaline lakes.

Gram-negative coccus-shaped obligate anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 393338

JCM 30661

@ref 20215

Last LPSN update 2026-05-29 08:39:48

Domain Archaea

Phylum Methanobacteriota

Class Halobacteria

Order Halobacteriales

Family Halobacteriaceae

Genus Halanaeroarchaeum

Species Halanaeroarchaeum sulfurireducens

Full scientific name Halanaeroarchaeum sulfurireducens Sorokin et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43637	negative	1-2 µm	0.5-1.5 µm	coccus-shaped

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)
43637	positive	optimum	37-40
43637	positive	maximum	46
67770	positive	growth	37

Culture pH

@ref	Ability	Type	PH
43637	positive	optimum	7-7.5
43637	positive	growth	6.7-8

Physiology and metabolism

Oxygen tolerance

43637

Oxygen toleranceobligate anaerobe

Spore formation

@ref	Spore formation	Confidence
125438		93.337

Halophily

@ref	Halophily level	Salt	Growth	Tested relation	Concentration
43637		NaCl	positive	growth	3-5 M
43637	extremely halophilic	NaCl	positive	optimum	4 M

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43637	30089 ChEBI	acetate	+	carbon source
43637	30089 ChEBI	acetate	+	electron donor
43637	28938 ChEBI	ammonium	+	nitrogen source
43637	15361 ChEBI	pyruvate	+	carbon source
43637	15361 ChEBI	pyruvate	+	electron donor

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	vitamin K metabolism	100	5 of 5
66794	ethanol fermentation	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	factor 420 biosynthesis	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	100	8 of 8
66794	chorismate metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	citric acid cycle	85.71	12 of 14
66794	phenylalanine metabolism	84.62	11 of 13
66794	pantothenate biosynthesis	83.33	5 of 6
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	palmitate biosynthesis	77.27	17 of 22
66794	vitamin B1 metabolism	76.92	10 of 13
66794	acetate fermentation	75	3 of 4
66794	proline metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	propanol degradation	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	Entner Doudoroff pathway	70	7 of 10
66794	purine metabolism	67.02	63 of 94
66794	acetoin degradation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	serine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	methionine metabolism	65.38	17 of 26
66794	glycolysis	64.71	11 of 17
66794	pyrimidine metabolism	64.44	29 of 45
66794	alanine metabolism	62.07	18 of 29
66794	leucine metabolism	61.54	8 of 13
66794	lipoate biosynthesis	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	3-chlorocatechol degradation	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	histidine metabolism	58.62	17 of 29
66794	arginine metabolism	58.33	14 of 24
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	cysteine metabolism	55.56	10 of 18
66794	non-pathway related	55.26	21 of 38
66794	pentose phosphate pathway	54.55	6 of 11
66794	oxidative phosphorylation	53.85	49 of 91
66794	urea cycle	53.85	7 of 13
66794	lysine metabolism	52.38	22 of 42
66794	lipid metabolism	51.61	16 of 31
66794	phenylmercury acetate degradation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	glutathione metabolism	50	7 of 14
66794	carotenoid biosynthesis	50	11 of 22
66794	ketogluconate metabolism	50	4 of 8
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	glycine metabolism	50	5 of 10
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	tyrosine metabolism	50	7 of 14
66794	CDP-diacylglycerol biosynthesis	50	1 of 2
66794	glycogen biosynthesis	50	2 of 4
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	tryptophan metabolism	47.37	18 of 38
66794	sulfate reduction	46.15	6 of 13
66794	mevalonate metabolism	42.86	3 of 7
66794	heme metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	vitamin B12 metabolism	41.18	14 of 34
66794	gallate degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	isoprenoid biosynthesis	38.46	10 of 26
66794	carnitine metabolism	37.5	3 of 8
66794	cholesterol biosynthesis	36.36	4 of 11
66794	degradation of pentoses	35.71	10 of 28
66794	tetrahydrofolate metabolism	35.71	5 of 14
66794	enterobactin biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	methanogenesis from CO2	33.33	4 of 12
66794	phenol degradation	30	6 of 20
66794	degradation of sugar acids	28	7 of 25
66794	degradation of hexoses	27.78	5 of 18
66794	vitamin B6 metabolism	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	polyamine pathway	26.09	6 of 23
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	lipid A biosynthesis	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Anoxic (anaerobic)	-
#Condition	#Saline	-
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
43637	anaerobic sediments of chloride-sulfate hypersaline lakes	Kulunda Steppe, Altai	Russia	RUS	Asia	2009-2013
67770	Mixed anaerobic sulfur-rich sediment samples from the Kukunda Steppe hypersaline lake	Altai	Russia	RUS

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
66792	ASM101111v1 assembly for Halanaeroarchaeum sulfurireducens HSR2	complete	GCA_001011115	2639763150	1604004	97.06

16S sequences

@ref	Description	Accession	Database
43637	Halanaeroarchaeum sulfurireducens strain HSR2 16S ribosomal RNA gene, partial sequence	KM875608

GC content

@ref	GC-content (mol%)	Method
43637	62.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Halanaeroarchaeum sulfurireducens HSR2
NCBI: GCA_001011115.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	76.18	yes
125438	anaerobic	anaerobicⓘ	no	69.20	no
125438	aerobic	aerobicⓘ	no	59.47	yes
125438	spore-forming	spore-formingⓘ	no	93.34	no
125438	thermophilic	thermophileⓘ	no	80.85	yes
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Halobacteria and Subcommittee on the taxonomy of Halomonadaceae. Minutes of the joint open meeting, 11 July 2017, Valencia, Spain.	Arahal DR, Oren A, Ventosa A.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002296	2017
	Halo(natrono)archaea from hypersaline lakes can utilize sulfoxides other than DMSO as electron acceptors for anaerobic respiration.	Sorokin DY, Roman P, Kolganova TV.	Extremophiles	10.1007/s00792-021-01219-y	2021
	Complete genome sequence of 'Halanaeroarchaeum sulfurireducens' M27-SA2, a sulfur-reducing and acetate-oxidizing haloarchaeon from the deep-sea hypersaline anoxic lake Medee.	Messina E, Sorokin DY, Kublanov IV, Toshchakov S, Lopatina A, Arcadi E, Smedile F, La Spada G, La Cono V, Yakimov MM.	Stand Genomic Sci	10.1186/s40793-016-0155-9	2016
Phylogeny	Proposed minimal standards for description of new taxa of the class Halobacteria.	Cui HL, Hou J, Amoozegar MA, Dyall-Smith ML, de la Haba RR, Minegishi H, Montalvo-Rodriguez R, Oren A, Sanchez-Porro C, Ventosa A, Vreeland RH.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006290	2024
	Pyridoxal 5'-phosphate synthesis and salvage in Bacteria and Archaea: predicting pathway variant distributions and holes.	Denise R, Babor J, Gerlt JA, de Crecy-Lagard V.	Microb Genom	10.1099/mgen.0.000926	2023
	Anaerobic carboxydotrophy in sulfur-respiring haloarchaea from hypersaline lakes.	Sorokin DY, Merkel AY, Messina E, Tugui C, Pabst M, Golyshin PN, Yakimov MM.	ISME J	10.1038/s41396-022-01206-x	2022
Metabolism	Sucrose Metabolism in Haloarchaea: Reassessment Using Genomics, Proteomics, and Metagenomics.	Williams TJ, Allen MA, Liao Y, Raftery MJ, Cavicchioli R.	Appl Environ Microbiol	10.1128/aem.02935-18	2019
Enzymology	Sulfur Respiration in a Group of Facultatively Anaerobic Natronoarchaea Ubiquitous in Hypersaline Soda Lakes.	Sorokin DY, Messina E, La Cono V, Ferrer M, Ciordia S, Mena MC, Toshchakov SV, Golyshin PN, Yakimov MM.	Front Microbiol	10.3389/fmicb.2018.02359	2018
Metabolism	Depthwise microbiome and isotopic profiling of a moderately saline microbial mat in a solar saltern.	Paul V, Banerjee Y, Ghosh P, Busi SB.	Sci Rep	10.1038/s41598-020-77622-w	2020
Phylogeny	Discovery of anaerobic lithoheterotrophic haloarchaea, ubiquitous in hypersaline habitats.	Sorokin DY, Messina E, Smedile F, Roman P, Damste JSS, Ciordia S, Mena MC, Ferrer M, Golyshin PN, Kublanov IV, Samarov NI, Toshchakov SV, La Cono V, Yakimov MM.	ISME J	10.1038/ismej.2016.203	2017
Metabolism	Elemental sulfur and acetate can support life of a novel strictly anaerobic haloarchaeon.	Sorokin DY, Kublanov IV, Gavrilov SN, Rojo D, Roman P, Golyshin PN, Slepak VZ, Smedile F, Ferrer M, Messina E, La Cono V, Yakimov MM.	ISME J	10.1038/ismej.2015.79	2016
	Scientific novelty beyond the experiment.	Hallsworth JE, Udaondo Z, Pedros-Alio C, Hofer J, Benison KC, Lloyd KG, Cordero RJB, de Campos CBL, Yakimov MM, Amils R.	Microb Biotechnol	10.1111/1751-7915.14222	2023
Phylogeny	Halanaeroarchaeum sulfurireducens gen. nov., sp. nov., the first obligately anaerobic sulfur-respiring haloarchaeon, isolated from a hypersaline lake.	Sorokin DY, Kublanov IV, Yakimov MM, Rijpstra WIC, Sinninghe Damste JS	Int J Syst Evol Microbiol	10.1099/ijsem.0.001041	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43637	Dimitry Y. Sorokin, Ilya V. Kublanov, Mikhail M. Yakimov, W. Irene C. Rijpstra, Jaap S. Sinninghe Damsté: Halanaeroarchaeum sulfurireducens gen. nov., sp. nov., the first obligately anaerobic sulfur-respiring haloarchaeon, isolated from a hypersaline lake. IJSEM 66: 2377 - 2381 2016 ( DOI 10.1099/ijsem.0.001041 , PubMed 27031647 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Halanaeroarchaeum sulfurireducens (JCM 30661, UNIQEM U935, HSR2)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_001011115

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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