Name: Shewanella halifaxensis A22-B3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14078

Type strain:

Species: Shewanella halifaxensis

Strain Designation: A22-B3, HAW-EB4

Culture col. no.: DSM 17350, CIP 109778, NCIMB 14093

Strain history: CIP <- 2007, DSMZ <- J.S. Zhao, Nat. Res. Council Canada, Montreal, Canada: strain HAW-EB4

NCBI tax ID(s): 271098 (species)

SI-ID 289875

DSM 17350, NCIMB 14093, CIP 109778

Section

Shewanella halifaxensis A22-B3 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from marine sediment of Emerald Basin.

Gram-negative
motile
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Shewanellaceae*
	Genus	*Shewanella*
	Species	**Shewanella halifaxensis**
	Full Scientific Name (LPSN)	Shewanella halifaxensis Zhao et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31570]	Gram stain	negative
[Ref.: #119793]	Gram stain	negative

[Ref.: #31570]	Cell length	02-03 µm

[Ref.: #31570]	Cell width	0.55-0.65 µm

[Ref.: #31570]	Cell shape	rod-shaped
[Ref.: #119793]	Cell shape	rod-shaped

[Ref.: #31570]	Motility	yes
[Ref.: #119793]	Motility	yes

[Ref.: #31570]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6930]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6930]

Culture medium growth

[Ref.: #6930]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6930]

Culture medium composition

expand / minimize

[Ref.: #37741]

Culture medium

Marine agar (MA)

[Ref.: #37741]

Culture medium growth

[Ref.: #37741]

Culture medium composition

expand / minimize

[Ref.: #119793]

Culture medium

CIP Medium 13

[Ref.: #119793]

Culture medium growth

[Ref.: #119793]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6930]	growth	15-22 °C
[Ref.: #31570]	growth	04-25 °C
[Ref.: #31570]	optimum	10 °C
[Ref.: #37741]	growth	15 °C

[Ref.: #6930]	Temperature range	psychrophilic
[Ref.: #31570]	Temperature range	psychrophilic
[Ref.: #37741]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #31570]			optimum	7
[Ref.: #31570]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31570]	Oxygen tolerance	aerobe
[Ref.: #119793]	Oxygen tolerance	obligate aerobe

[Ref.: #31570]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31570]		NaCl		growth	0.5-4	%
[Ref.: #31570]		NaCl		optimum	2	%

[Ref.: #31570]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31570]	arabinose ChEBI	+	carbon source
[Ref.: #31570]	cellobiose ChEBI	+	carbon source
[Ref.: #31570]	fructose ChEBI	+	carbon source
[Ref.: #31570]	fucose ChEBI	+	carbon source
[Ref.: #31570]	galactose ChEBI	+	carbon source
[Ref.: #31570]	L-arabitol ChEBI	+	carbon source
[Ref.: #31570]	lactose ChEBI	+	carbon source
[Ref.: #31570]	maltose ChEBI	+	carbon source
[Ref.: #31570]	mannitol ChEBI	+	carbon source
[Ref.: #31570]	mannose ChEBI	+	carbon source
[Ref.: #31570]	melibiose ChEBI	+	carbon source
[Ref.: #119793]	nitrate ChEBI	+	reduction
[Ref.: #119793]	nitrite ChEBI	+	reduction
[Ref.: #31570]	raffinose ChEBI	+	carbon source
[Ref.: #31570]	rhamnose ChEBI	+	carbon source
[Ref.: #31570]	sorbitol ChEBI	+	carbon source
[Ref.: #31570]	sucrose ChEBI	+	carbon source
[Ref.: #31570]	trehalose ChEBI	+	carbon source
[Ref.: #31570]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119793]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31570]	catalase	+	1.11.1.6
[Ref.: #119793]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31570]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119793]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #31570]	urease	+	3.5.1.5
[Ref.: #119793]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119793]	12104	-	+	+	+	-	+	-	-	+	+	+	+	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6930]	Sample type/isolated from	marine sediment of Emerald Basin
[Ref.: #6930]	Geographic location (country and/or sea, region)	Halifax, Atlantic Ocean
[Ref.: #6930]	Country	Canada
[Ref.: #6930]	Country ISO 3 Code	CAN
[Ref.: #6930]	Continent	North America

[Ref.: #119793]	Sample type/isolated from	Environment, Marine sediment of Emerald Basin
[Ref.: #119793]	Geographic location (country and/or sea, region)	Halifax, Nova Scotia
[Ref.: #119793]	Country	Canada
[Ref.: #119793]	Country ISO 3 Code	CAN
[Ref.: #119793]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY579751 (>99% sequence identity) for Shewanella from Microbeatlas

Aquatic Samples	2532
Soil Samples	168
Animal Samples	600
Plant Samples	29
Total Samples	3329

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6930]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119793]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31570]	Shewanella halifaxensis strain HAW-EB4 16S ribosomal RNA gene, partial sequence	AY579751	1293	GenBank	458817 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Shewanella halifaxensis HAW-EB4	GCA_000019185	complete	GenBank	458817 tax ID	*
[Ref.: #66792]	Shewanella halifaxensis HAW-EB4	458817.8	complete	PATRIC	458817 tax ID	*
[Ref.: #66792]	Shewanella halifaxensis HAW-EB4	641522648	complete	JGI IMG/M	458817 tax ID	*

[Ref.: #31570]

GC-content

45 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Shewanella halifaxensis HAW-EB4 HAW-EB4 NCBI: GCA_000019185.1
[Ref.: #69480]	motile	yes	91.482	yes
[Ref.: #69480]	flagellated	yes	81.877	no
[Ref.: #69480]	gram-positive	no	98.834	yes
[Ref.: #69480]	anaerobic	no	95.854	no
[Ref.: #69480]	aerobic	no	74.826	yes
[Ref.: #69480]	halophile	yes	53.659	no
[Ref.: #69480]	spore-forming	no	95.693	yes
[Ref.: #69480]	thermophile	no	99.06	yes
[Ref.: #69480]	glucose-util	yes	88.755	no
[Ref.: #69480]	glucose-ferment	no	57.795	no

Availability in culture collections External links

[Ref.: #6930]

Culture collection no.

DSM 17350, CIP 109778, NCIMB 14093

[Ref.: #83247]

SI-ID 289875

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Characterization of a cryptic plasmid pSFKW33 from Shewanella sp. 33B.	Werbowy K, Cieslinski H, Kur J	Plasmid	10.1016/j.plasmid.2009.03.003	2009	*
Metabolism	Regulation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) metabolism in Shewanella halifaxensis HAW-EB4 by terminal electron acceptor and involvement of c-type cytochrome.	Zhao JS, Manno D, Hawari J	Microbiology (Reading)	10.1099/mic.0.2007/013409-0	2008	*
Phylogeny	Shewanella halifaxensis sp. nov., a novel obligately respiratory and denitrifying psychrophile.	Zhao JS, Manno D, Leggiadro C, O'Neil D, Hawari J	Int J Syst Evol Microbiol	10.1099/ijs.0.63829-0	2006	*

References

#6930

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17350

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31570

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27859 (see below)

#37741 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#83247

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID289875.1 )

#119793 Collection of Institut Pasteur ; Curators of the CIP; CIP 109778

#27859

IJSEM 205 2006 ( DOI 10.1099/ijs.0.63829-0 , PubMed 16403888 )

* These data were automatically processed and therefore are not curated

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Shewanella halifaxensis HAW-EB4 HAW-EB4

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