Name: Sphingomonas olei K-1-16
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140698

Type strain:

Species: Sphingomonas olei

Strain Designation: K-1-16

Culture col. no.: KEMB 9005-450, KACC 19002, JCM 31674

Strain history: J. Kim; Kyonggi Univ., South Korea; K-1-16.

NCBI tax ID(s): 1886787 (species)

Other strains from Sphingomonas olei

S. olei NM83_B4-11, DSM 110168

Section

Sphingomonas olei K-1-16 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from Oil contaminated soil at Biratnagar.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Sphingomonadales*
	Family	*Sphingomonadaceae*
	Genus	*Sphingomonas*
	Species	**Sphingomonas olei**
	Full Scientific Name (LPSN)	Sphingomonas olei Chaudhary and Kim 2017

Information on morphological and physiological properties Morphology

[Ref.: #43468]	Gram stain	negative

[Ref.: #43468]	Cell length	1.2-1.7 µm

[Ref.: #43468]	Cell width	0.5-0.8 µm

[Ref.: #43468]	Cell shape	rod-shaped

[Ref.: #43468]	Motility	no

[Ref.: #43468]	Colony size	0.7-1.3 mm
[Ref.: #43468]	Colony color	Yellow
[Ref.: #43468]	Colony shape	circular
[Ref.: #43468]	Incubation period	5 days
[Ref.: #43468]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43468]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43468]

Culture medium growth

[Ref.: #43468]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #43468]

Culture medium growth

[Ref.: #43468]

Culture medium

LBA

[Ref.: #43468]

Culture medium growth

[Ref.: #43468]

Culture medium

Marine agar (MA)

[Ref.: #43468]

Culture medium growth

		Temperature
[Ref.: #43468]	growth	15-45 °C
[Ref.: #43468]	optimum	20-40 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #67770]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43468]			optimum	6.5-8
[Ref.: #43468]			growth	5.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43468]

Oxygen tolerance

aerobe

[Ref.: #43468]

Ability of spore formation

[Ref.: #43468]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43468]		NaCl		growth	0-5	%(w/v)
[Ref.: #43468]		NaCl		optimum	0	%(w/v)

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43468]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43468]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #43468]	4-hydroxybenzoate ChEBI	+	assimilation
[Ref.: #43468]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	assimilation
[Ref.: #43468]	adipate ChEBI	+/-	assimilation
[Ref.: #43468]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43468]	casein	-	hydrolysis
[Ref.: #43468]	chitin ChEBI	-	hydrolysis
[Ref.: #43468]	D-glucose ChEBI	+	assimilation
[Ref.: #43468]	D-mannitol ChEBI	-	assimilation
[Ref.: #43468]	D-mannose ChEBI	+	assimilation
[Ref.: #43468]	D-ribose ChEBI	-	assimilation
[Ref.: #43468]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43468]	decanoate ChEBI	-	assimilation
[Ref.: #43468]	dna ChEBI	+	hydrolysis
[Ref.: #43468]	esculin ferric citrate	+	assimilation
[Ref.: #43468]	esculin ferric citrate	+	hydrolysis
[Ref.: #43468]	gelatin ChEBI	+	assimilation
[Ref.: #43468]	gelatin ChEBI	+	hydrolysis
[Ref.: #43468]	glucose	-	fermentation
[Ref.: #43468]	glucose ChEBI	-	fermentation
[Ref.: #43468]	glycogen ChEBI	-	assimilation
[Ref.: #43468]	itaconate ChEBI	-	assimilation
[Ref.: #43468]	L-alanine ChEBI	+	assimilation
[Ref.: #43468]	L-arabinose ChEBI	-	assimilation
[Ref.: #43468]	L-arginine ChEBI	-	fermentation
[Ref.: #43468]	L-fucose ChEBI	-	assimilation
[Ref.: #43468]	L-histidine ChEBI	-	assimilation
[Ref.: #43468]	L-proline ChEBI	-	assimilation
[Ref.: #43468]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43468]	L-serine ChEBI	-	assimilation
[Ref.: #43468]	L-tryptophan ChEBI	-	fermentation
[Ref.: #43468]	lactate ChEBI	-	assimilation
[Ref.: #43468]	malate ChEBI	-	assimilation
[Ref.: #43468]	maltose ChEBI	-	assimilation
[Ref.: #43468]	melibiose ChEBI	-	assimilation
[Ref.: #43468]	myo-inositol ChEBI	-	assimilation
[Ref.: #43468]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43468]	nitrate ChEBI	+	reduction
[Ref.: #43468]	phenylacetate ChEBI	-	assimilation
[Ref.: #43468]	potassium 2-dehydro-D-gluconate	+	assimilation
[Ref.: #43468]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #43468]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43468]	propionate ChEBI	+	assimilation
[Ref.: #43468]	salicin ChEBI	-	assimilation
[Ref.: #43468]	sodium acetate ChEBI	+	assimilation
[Ref.: #43468]	sodium citrate ChEBI	-	assimilation
[Ref.: #43468]	sodium malonate ChEBI	-	assimilation
[Ref.: #43468]	starch ChEBI	-	hydrolysis
[Ref.: #43468]	suberic acid ChEBI	+	assimilation
[Ref.: #43468]	sucrose ChEBI	-	assimilation
[Ref.: #43468]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43468]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43468]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43468]	urea ChEBI	-	fermentation
[Ref.: #43468]	valerate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43468]		hydrogen sulfide ChEBI	no
[Ref.: #43468]		indole ChEBI	no

	Physiological tests	Metabolite	Methylred-test
[Ref.: #43468]		glucose ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #43468]	acid phosphatase	+	3.1.3.2
[Ref.: #43468]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43468]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43468]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43468]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43468]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43468]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43468]	beta-galactosidase	-	3.2.1.23
[Ref.: #43468]	beta-glucosidase	+	3.2.1.21
[Ref.: #43468]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43468]	catalase	+	1.11.1.6
[Ref.: #43468]	cystine arylamidase	+	3.4.11.3
[Ref.: #43468]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43468]	esterase (C 4)	+
[Ref.: #43468]	esterase Lipase (C 8)	+
[Ref.: #43468]	leucine arylamidase	+	3.4.11.1
[Ref.: #43468]	lipase (C 14)	+/-
[Ref.: #43468]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43468]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43468]	trypsin	+	3.4.21.4
[Ref.: #43468]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43468]

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	50.3
C16:0	17.8
C16:1ω7c / C16:1ω6c	10.2
11-methyl C18:1ω7c	8.4
C14:0 2OH	8.3
C16:1ω5c	2.5
C17:1ω6c	2.2
C18:0	0.9
C14:0	0.8
C18:1ω5c	0.7
C16:1ω11c	0.5
C17:0	0.4
C15:0 2OH	0.4
C19:0 cyclo ω8c	0.3
C17:1ω8c	0.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43468]	Sample type/isolated from	Oil contaminated soil at Biratnagar
[Ref.: #43468]	Geographic location (country and/or sea, region)	Biratnagar, Morang
[Ref.: #43468]	Country	Nepal
[Ref.: #43468]	Country ISO 3 Code	NPL
[Ref.: #43468]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Oil-contaminated soil at Biratnagar
[Ref.: #67770]	Geographic location (country and/or sea, region)	Morang
[Ref.: #67770]	Country	Nepal
[Ref.: #67770]	Country ISO 3 Code	NPL
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX672814 (>99% sequence identity) for Sphingomonas sp. from Microbeatlas

Aquatic Samples	8511
Soil Samples	7683
Animal Samples	13486
Plant Samples	8053
Total Samples	37733

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43468]	16S rRNA gene sequence	KX672814		GenBank

[Ref.: #67770]

GC-content

64.8 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43468]

Culture collection no.

KEMB 9005-450, KACC 19002, JCM 31674

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphingomonas radiodurans sp. nov., a novel radiation-resistant bacterium isolated from the north slope of Mount Everest.	Liu Y, Chen T, Cui X, Xu Y, Hu S, Zhao Y, Zhang W, Liu G, Zhang G	Int J Syst Evol Microbiol	10.1099/ijsem.0.005312	2022	*
Phylogeny	Sphingomonas olei sp. nov., with the ability to degrade aliphatic hydrocarbons, isolated from oil-contaminated soil.	Chaudhary DK, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002010	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43468

Dhiraj Kumar Chaudhary, Jaisoo Kim: Sphingomonas olei sp. nov., with the ability to degrade aliphatic hydrocarbons, isolated from oil-contaminated soil. IJSEM 67: 2731 - 2738 2017 ( DOI 10.1099/ijsem.0.002010 , PubMed 28792375 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Sphingomonas olei in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF