Name: Shewanella denitrificans OS217
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14068

Type strain:

Species: Shewanella denitrificans

Strain Designation: OS217

Culture col. no.: DSM 15013, LMG 21692, CIP 107825

Strain history: CIP <- 2003, I. Brettar, GBF, Braunschweig, Germany: strain OS217

NCBI tax ID(s): 192073 (species)

SI-ID 89621

LMG 21692, DSM 15013, ATCC BAA-1090, CIP 107825

Other strains from Shewanella denitrificans

S. denitrificans OS226, DSM 15014

Section

Shewanella denitrificans OS217 is an obligate aerobe, motile bacterium that was isolated from water sample, 130 m depth.

motile
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Shewanellaceae*
	Genus	*Shewanella*
	Species	**Shewanella denitrificans**
	Full Scientific Name (LPSN)	Shewanella denitrificans Brettar et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #118906]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.994

[Ref.: #118906]	Cell shape	rod-shaped

[Ref.: #118906]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.98

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5667]

Culture medium

HALF STRENGTH BACTO MARINE BROTH (DSMZ Medium 514a)

[Ref.: #5667]

Culture medium growth

[Ref.: #5667]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5667]

Culture medium composition

expand / minimize

[Ref.: #39951]

Culture medium

Marine agar (MA)

[Ref.: #39951]

Culture medium growth

[Ref.: #39951]

Culture medium composition

expand / minimize

[Ref.: #118906]

Culture medium

CIP Medium 186

[Ref.: #118906]

Culture medium growth

[Ref.: #118906]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118906]

Culture medium

CIP Medium 13

[Ref.: #118906]

Culture medium growth

[Ref.: #118906]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5667]		growth	20-25 °C
[Ref.: #39951]		growth	25 °C
[Ref.: #118906]		growth	5-30 °C
[Ref.: #118906]	no	growth	37 °C
[Ref.: #118906]	no	growth	41 °C

[Ref.: #39951]	Temperature range	mesophilic
[Ref.: #118906]	Temperature range	mesophilic
[Ref.: #118906]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118906]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.974

	Salt	Tested relation		Salt conc.
[Ref.: #118906]	NaCl		growth	2-4	%
[Ref.: #118906]	NaCl	no	growth	0	%
[Ref.: #118906]	NaCl	no	growth	6	%
[Ref.: #118906]	NaCl	no	growth	8	%
[Ref.: #118906]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #118906]	citrate ChEBI	-	carbon source
[Ref.: #118906]	esculin ChEBI	+	hydrolysis
[Ref.: #118906]	nitrate ChEBI	+	reduction
[Ref.: #118906]	nitrite ChEBI	+	reduction
[Ref.: #118906]	sodium thiosulfate ChEBI	-	builds gas from

	Metabolite production	Metabolite	Production
[Ref.: #118906]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118906]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118906]	amylase	+
[Ref.: #118906]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118906]	caseinase	+	3.4.21.50
[Ref.: #118906]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118906]	gelatinase	+
[Ref.: #118906]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #118906]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118906]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118906]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118906]	oxidase	+
[Ref.: #118906]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118906]	tryptophan deaminase	-
[Ref.: #118906]	tween esterase	+
[Ref.: #118906]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118906]	2454	not determinedn.d.	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118906]	10679	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5667]	Sample type/isolated from	water sample, 130 m depth
[Ref.: #5667]	Geographic location (country and/or sea, region)	Baltic Sea

[Ref.: #118906]	Sample type/isolated from	Environment, Water samples
[Ref.: #118906]	Geographic location (country and/or sea, region)	Gotland Deep, a basin with anoxic deep water in the central baltic
[Ref.: #118906]	Country	Baltic Sea
[Ref.: #118906]	Isolation date	1986
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5667]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118906]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5667]	Shewanella denitrificans partial 16S rRNA gene, strain OS-217	AJ311964	1500	ENA	318161 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Shewanella denitrificans OS217	GCA_000013765	complete	GenBank	318161 tax ID	*
[Ref.: #66792]	Shewanella denitrificans OS217	318161.16	complete	PATRIC	318161 tax ID	*
[Ref.: #66792]	Shewanella denitrificans OS217	2524023135	complete	JGI IMG/M	318161 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Shewanella denitrificans OS217 PATRIC: 318161.16
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Shewanella denitrificans OS217 OS217 NCBI: GCA_000013765.1
[Ref.: #69480]	motile	yes	91.316	no
[Ref.: #69480]	flagellated	yes	85.524	no
[Ref.: #69480]	gram-positive	no	98.788	no
[Ref.: #69480]	anaerobic	no	99.039	no
[Ref.: #69480]	halophile	no	53.064	no
[Ref.: #69480]	spore-forming	no	94.629	no
[Ref.: #69480]	thermophile	no	99.46	yes
[Ref.: #69480]	glucose-util	yes	91.852	no
[Ref.: #69480]	aerobic	yes	63.199	no
[Ref.: #69480]	glucose-ferment	no	72.256	no

Availability in culture collections External links

[Ref.: #5667]

Culture collection no.

DSM 15013, LMG 21692, CIP 107825

[Ref.: #83237]

SI-ID 89621

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	Spectroscopic characterization and mechanistic investigation of P-methyl transfer by a radical SAM enzyme from the marine bacterium Shewanella denitrificans OS217.	Allen KD, Wang SC	Biochim Biophys Acta	10.1016/j.bbapap.2014.09.009	2014	*
Metabolism	Comparisons of Shewanella strains based on genome annotations, modeling, and experiments.	Ong WK, Vu TT, Lovendahl KN, Llull JM, Serres MH, Romine MF, Reed JL	BMC Syst Biol	10.1186/1752-0509-8-31	2014	*
Phylogeny	Shewanella aestuarii sp. nov., a marine bacterium isolated from a tidal flat.	Park HY, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijs.0.055178-0	2013	*
Metabolism	Shewanella spp. Use acetate as an electron donor for denitrification but not ferric iron or fumarate reduction.	Yoon S, Sanford RA, Loffler FE	Appl Environ Microbiol	10.1128/AEM.03872-12	2013	*
Enzymology	The periplasmic nitrate reductase in Shewanella: the resolution, distribution and functional implications of two NAP isoforms, NapEDABC and NapDAGHB.	Simpson PJL, Richardson DJ, Codd R	Microbiology (Reading)	10.1099/mic.0.034421-0	2009	*
Metabolism	Biogenic formation of As-S nanotubes by diverse Shewanella strains.	Jiang S, Lee JH, Kim MG, Myung NV, Fredrickson JK, Sadowsky MJ, Hur HG	Appl Environ Microbiol	10.1128/AEM.00450-09	2009	*
Phylogeny	Shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxic-anoxic interface of the Gotland Deep in the central Baltic Sea.	Brettar I, Christen R, Hofle MG	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2211	2002	*
	Redox Sensing within the Genus Shewanella.	Harris HW, Sanchez-Andrea I, McLean JS, Salas EC, Tran W, El-Naggar MY, Nealson KH	Front Microbiol	10.3389/fmicb.2017.02568	2018	*

References

#5667

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15013

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39951 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83237

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID89621.1 )

#118906 Collection of Institut Pasteur ; Curators of the CIP; CIP 107825
* These data were automatically processed and therefore are not curated

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Shewanella denitrificans OS217

Shewanella denitrificans OS217 OS217

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