Name: Puzihella rosea MI-28
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140646

Type strain:

Species: Puzihella rosea

Strain Designation: MI-28

Culture col. no.: KCTC 29239, LMG 27848, BCRC 80688

Strain history: <- Wen Ming Chen,Taiwan

NCBI tax ID(s): 1377991 (species)

Other strains from Puzihella rosea

P. rosea MWH-Ta3, DSM 102236

Section

Puzihella rosea MI-28 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from freshwater sample.

colony-forming
Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Puzihella*
	Species	**Puzihella rosea**
	Full Scientific Name (LPSN)	Puzihella rosea Sheu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43286]	Gram stain	positive

[Ref.: #43286]	Cell length	0.6-0.9 µm

[Ref.: #43286]	Cell width	0.1-0.2 µm

[Ref.: #43286]	Cell shape	rod-shaped

[Ref.: #43286]	Motility	no

[Ref.: #43286]	Colony size	0.2-0.4 mm
[Ref.: #43286]	Colony color	pink
[Ref.: #43286]	Colony shape	circular
[Ref.: #43286]	Incubation period	2 days
[Ref.: #43286]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43286]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43286]

Culture medium growth

		Temperature
[Ref.: #43286]	optimum	20-30 °C
[Ref.: #43286]	growth	15-40 °C
[Ref.: #67771]	growth	25 °C

[Ref.: #67771]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43286]			optimum	7.0-9.0
[Ref.: #43286]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43286]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43286]	Type of spore	spore
[Ref.: #43286]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43286]		NaCl		growth	0-1.0	%(w/v)
[Ref.: #43286]		NaCl		optimum	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43286]	(S)-lactate ChEBI	-	respiration
[Ref.: #43286]	2,3-butanediol ChEBI	-	respiration
[Ref.: #43286]	2-deoxyadenosine ChEBI	-	respiration
[Ref.: #43286]	2-hydroxybutyrate ChEBI	-	respiration
[Ref.: #43286]	2-oxogluconate	-	assimilation
[Ref.: #43286]	2-oxoglutarate ChEBI	-	respiration
[Ref.: #43286]	2-oxopentanoate ChEBI	+	respiration
[Ref.: #43286]	3-hydroxybutyrate ChEBI	-	respiration
[Ref.: #43286]	3-O-methyl-D-glucose ChEBI	-	respiration
[Ref.: #43286]	4-hydroxybutyrate ChEBI	-	respiration
[Ref.: #43286]	4-hydroxyphenylacetic acid ChEBI	-	respiration
[Ref.: #43286]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #43286]	acetate ChEBI	-	respiration
[Ref.: #43286]	adenosine ChEBI	-	respiration
[Ref.: #43286]	adipate ChEBI	-	assimilation
[Ref.: #43286]	alpha-cyclodextrin ChEBI	-	respiration
[Ref.: #43286]	alpha-D-glucose ChEBI	+	respiration
[Ref.: #43286]	alpha-lactose ChEBI	-	respiration
[Ref.: #43286]	amp ChEBI	-	respiration
[Ref.: #43286]	amygdalin ChEBI	-	assimilation
[Ref.: #43286]	amygdalin ChEBI	-	respiration
[Ref.: #43286]	arabinose ChEBI	-	assimilation
[Ref.: #43286]	arbutin ChEBI	-	assimilation
[Ref.: #43286]	arbutin ChEBI	-	respiration
[Ref.: #43286]	beta-cyclodextrin ChEBI	-	respiration
[Ref.: #43286]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43286]	casein	-	hydrolysis
[Ref.: #43286]	cellobiose ChEBI	-	assimilation
[Ref.: #43286]	cellobiose ChEBI	-	respiration
[Ref.: #43286]	chitin ChEBI	-	hydrolysis
[Ref.: #43286]	citrate ChEBI	-	assimilation
[Ref.: #43286]	corn oil	-	hydrolysis
[Ref.: #43286]	D-alanine ChEBI	-	respiration
[Ref.: #43286]	D-arabinose ChEBI	-	assimilation
[Ref.: #43286]	D-arabitol ChEBI	-	assimilation
[Ref.: #43286]	D-arabitol ChEBI	-	respiration
[Ref.: #43286]	D-fructose ChEBI	+	builds acid from
[Ref.: #43286]	D-fructose ChEBI	+	respiration
[Ref.: #43286]	D-fructose 6-phosphate ChEBI	-	respiration
[Ref.: #43286]	D-fucose ChEBI	-	assimilation
[Ref.: #43286]	D-galactose ChEBI	+	builds acid from
[Ref.: #43286]	D-galactose ChEBI	-	respiration
[Ref.: #43286]	D-galacturonic acid ChEBI	-	respiration
[Ref.: #43286]	D-gluconate ChEBI	-	respiration
[Ref.: #43286]	D-glucose ChEBI	+	builds acid from
[Ref.: #43286]	D-glucose 6-phosphate ChEBI	-	respiration
[Ref.: #43286]	D-lyxose ChEBI	-	assimilation
[Ref.: #43286]	D-malate ChEBI	-	respiration
[Ref.: #43286]	D-mannitol ChEBI	+	builds acid from
[Ref.: #43286]	D-mannitol ChEBI	-	respiration
[Ref.: #43286]	D-mannose ChEBI	+	builds acid from
[Ref.: #43286]	D-mannose ChEBI	+	respiration
[Ref.: #43286]	D-psicose ChEBI	+	respiration
[Ref.: #43286]	D-ribose ChEBI	-	assimilation
[Ref.: #43286]	D-ribose ChEBI	-	respiration
[Ref.: #43286]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #43286]	D-sorbitol ChEBI	-	respiration
[Ref.: #43286]	D-tagatose ChEBI	-	assimilation
[Ref.: #43286]	D-tagatose ChEBI	-	respiration
[Ref.: #43286]	D-trehalose ChEBI	-	respiration
[Ref.: #43286]	D-xylose ChEBI	-	assimilation
[Ref.: #43286]	D-xylose ChEBI	-	respiration
[Ref.: #43286]	decanoate ChEBI	-	assimilation
[Ref.: #43286]	dextrin ChEBI	-	respiration
[Ref.: #43286]	dna ChEBI	-	hydrolysis
[Ref.: #43286]	erythritol ChEBI	-	assimilation
[Ref.: #43286]	esculin ChEBI	-	hydrolysis
[Ref.: #43286]	esculin ChEBI	-	assimilation
[Ref.: #43286]	galactitol ChEBI	-	assimilation
[Ref.: #43286]	gelatin ChEBI	+	hydrolysis
[Ref.: #43286]	gentiobiose ChEBI	-	assimilation
[Ref.: #43286]	gentiobiose ChEBI	-	respiration
[Ref.: #43286]	gluconate ChEBI	-	assimilation
[Ref.: #43286]	glucose ChEBI	-	fermentation
[Ref.: #43286]	glucose ChEBI	-	assimilation
[Ref.: #43286]	glucose 1-phosphate ChEBI	-	respiration
[Ref.: #43286]	glycerol ChEBI	-	assimilation
[Ref.: #43286]	glycerol ChEBI	-	respiration
[Ref.: #43286]	glycerol 1-phosphate ChEBI	-	respiration
[Ref.: #43286]	glycogen ChEBI	-	assimilation
[Ref.: #43286]	glycogen ChEBI	-	respiration
[Ref.: #43286]	glycyl-l-glutamate ChEBI	-	respiration
[Ref.: #43286]	inosine ChEBI	+	respiration
[Ref.: #43286]	inulin ChEBI	-	assimilation
[Ref.: #43286]	inulin ChEBI	-	respiration
[Ref.: #43286]	L-alaninamide ChEBI	+	respiration
[Ref.: #43286]	L-alanine ChEBI	-	respiration
[Ref.: #43286]	L-alanylglycine ChEBI	-	respiration
[Ref.: #43286]	L-arabinose ChEBI	-	assimilation
[Ref.: #43286]	L-arabinose ChEBI	-	respiration
[Ref.: #43286]	L-arabitol ChEBI	-	assimilation
[Ref.: #43286]	L-asparagine ChEBI	-	respiration
[Ref.: #43286]	L-fucose ChEBI	-	assimilation
[Ref.: #43286]	L-fucose ChEBI	-	respiration
[Ref.: #43286]	L-glutamate ChEBI	-	respiration
[Ref.: #43286]	L-malate ChEBI	-	respiration
[Ref.: #43286]	L-pyroglutamic acid ChEBI	-	respiration
[Ref.: #43286]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43286]	L-rhamnose ChEBI	-	respiration
[Ref.: #43286]	L-serine ChEBI	-	respiration
[Ref.: #43286]	L-sorbose ChEBI	+	builds acid from
[Ref.: #43286]	L-xylose ChEBI	-	assimilation
[Ref.: #43286]	lactamide ChEBI	-	respiration
[Ref.: #43286]	lactose ChEBI	-	assimilation
[Ref.: #43286]	lactulose ChEBI	-	respiration
[Ref.: #43286]	malate ChEBI	-	assimilation
[Ref.: #43286]	maltose ChEBI	-	assimilation
[Ref.: #43286]	maltose ChEBI	+	builds acid from
[Ref.: #43286]	maltose ChEBI	-	respiration
[Ref.: #43286]	maltotriose ChEBI	-	respiration
[Ref.: #43286]	mannan ChEBI	-	respiration
[Ref.: #43286]	mannitol ChEBI	-	assimilation
[Ref.: #43286]	mannose ChEBI	-	assimilation
[Ref.: #43286]	melezitose ChEBI	+	builds acid from
[Ref.: #43286]	melezitose ChEBI	-	respiration
[Ref.: #43286]	melibiose ChEBI	-	assimilation
[Ref.: #43286]	melibiose ChEBI	-	respiration
[Ref.: #43286]	methyl (R)-lactate ChEBI	-	respiration
[Ref.: #43286]	methyl alpha-D-galactoside ChEBI	-	respiration
[Ref.: #43286]	methyl alpha-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #43286]	methyl alpha-D-glucopyranoside ChEBI	-	respiration
[Ref.: #43286]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #43286]	methyl alpha-D-mannoside ChEBI	-	respiration
[Ref.: #43286]	methyl beta-D-galactoside ChEBI	-	respiration
[Ref.: #43286]	methyl beta-D-glucopyranoside ChEBI	-	respiration
[Ref.: #43286]	methyl beta-D-xylopyranoside ChEBI	-	assimilation
[Ref.: #43286]	methyl pyruvate ChEBI	+	respiration
[Ref.: #43286]	monomethyl succinate ChEBI	-	respiration
[Ref.: #43286]	myo-inositol ChEBI	+	builds acid from
[Ref.: #43286]	myo-inositol ChEBI	-	respiration
[Ref.: #43286]	N-acetyl-beta-D-mannosamine ChEBI	-	respiration
[Ref.: #43286]	N-acetyl-L-glutamate ChEBI	-	respiration
[Ref.: #43286]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43286]	N-acetylglucosamine ChEBI	-	respiration
[Ref.: #43286]	nitrate ChEBI	-	reduction
[Ref.: #43286]	palatinose ChEBI	-	respiration
[Ref.: #43286]	phenylacetate ChEBI	-	assimilation
[Ref.: #43286]	propionate ChEBI	-	respiration
[Ref.: #43286]	putrescine ChEBI	-	respiration
[Ref.: #43286]	pyruvate ChEBI	-	respiration
[Ref.: #43286]	raffinose ChEBI	+	builds acid from
[Ref.: #43286]	raffinose ChEBI	-	respiration
[Ref.: #43286]	ribitol ChEBI	-	assimilation
[Ref.: #43286]	salicin ChEBI	-	assimilation
[Ref.: #43286]	salicin ChEBI	-	respiration
[Ref.: #43286]	sedoheptulosan ChEBI	-	respiration
[Ref.: #43286]	stachyose ChEBI	-	respiration
[Ref.: #43286]	starch ChEBI	+	hydrolysis
[Ref.: #43286]	starch ChEBI	+	builds acid from
[Ref.: #43286]	succinamate ChEBI	-	respiration
[Ref.: #43286]	succinate ChEBI	-	respiration
[Ref.: #43286]	sucrose ChEBI	+	builds acid from
[Ref.: #43286]	sucrose ChEBI	+	respiration
[Ref.: #43286]	thymidine ChEBI	-	respiration
[Ref.: #43286]	thymidine 5'-monophosphate ChEBI	-	respiration
[Ref.: #43286]	trehalose ChEBI	+	builds acid from
[Ref.: #43286]	turanose ChEBI	+	builds acid from
[Ref.: #43286]	turanose ChEBI	-	respiration
[Ref.: #43286]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43286]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43286]	tween 40 ChEBI	-	respiration
[Ref.: #43286]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43286]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43286]	tween 80 ChEBI	-	respiration
[Ref.: #43286]	uridine ChEBI	+	respiration
[Ref.: #43286]	uridine 5'-monophosphate ChEBI	-	respiration
[Ref.: #43286]	urocanic acid ChEBI	+	respiration
[Ref.: #43286]	xylitol ChEBI	-	assimilation
[Ref.: #43286]	xylitol ChEBI	-	respiration
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43286]	chloramphenicol ChEBI	yes	30	µg
[Ref.: #43286]	gentamicin ChEBI	yes	10	µg
[Ref.: #43286]	kanamycin ChEBI	yes	30	µg
[Ref.: #43286]	nalidixic acid ChEBI				yes	30	µg
[Ref.: #43286]	novobiocin ChEBI	yes	30	µg
[Ref.: #43286]	penicillin g ChEBI	yes	10	Unit
[Ref.: #43286]	rifampicin ChEBI	yes	5	µg
[Ref.: #43286]	streptomycin ChEBI	yes	10	µg
[Ref.: #43286]	tetracycline ChEBI	yes	30	µg

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance	Concentration
[Ref.: #43286]		26	sulfamethoxazole ChEBI	yes	23.75	µg
[Ref.: #43286]		26	trimethoprim ChEBI	yes	1.25	µg

	Metabolite production	Metabolite	Production
[Ref.: #43286]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43286]	acid phosphatase	+	3.1.3.2
[Ref.: #43286]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43286]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43286]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43286]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43286]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43286]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43286]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43286]	beta-galactosidase	-	3.2.1.23
[Ref.: #43286]	beta-glucosidase	-	3.2.1.21
[Ref.: #43286]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43286]	catalase	+	1.11.1.6
[Ref.: #43286]	cystine arylamidase	+	3.4.11.3
[Ref.: #43286]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43286]	esterase (C 4)	+
[Ref.: #43286]	esterase Lipase (C 8)	+
[Ref.: #43286]	leucine arylamidase	+	3.4.11.1
[Ref.: #43286]	lipase (C 14)	-
[Ref.: #43286]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43286]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43286]	trypsin	-	3.4.21.4
[Ref.: #43286]	urease	-	3.5.1.5
[Ref.: #43286]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43286]	Sample type/isolated from	freshwater sample
[Ref.: #43286]	Geographic location (country and/or sea, region)	River Puzih, in the vicinity of Chiayi County, Taiwan
[Ref.: #43286]	Country	Taiwan
[Ref.: #43286]	Country ISO 3 Code	TWN
[Ref.: #43286]	Continent	Asia
[Ref.: #43286]	Geographic location	23.0000°/120.0000°

[Ref.: #67771]	Sample type/isolated from	From fresh water
[Ref.: #67771]	Geographic location (country and/or sea, region)	Chiayi County Puzih River
[Ref.: #67771]	Country	Taiwan
[Ref.: #67771]	Country ISO 3 Code	TWN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HG323815 (>99% sequence identity) for Puzihella rosea subclade from Microbeatlas

Aquatic Samples	670
Soil Samples	42
Animal Samples	57
Plant Samples	14
Total Samples	783

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43286]	16S rRNA gene sequences	HG323815	1400	ENA	1377991 tax ID

[Ref.: #43286]

GC-content

62.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43286]

Culture collection no.

KCTC 29239, LMG 27848, BCRC 80688

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Puzihella rosea gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from freshwater.	Sheu SY, Liu LP, Chen WM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001967	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43286

Shih-Yi Sheu, Li-Ping Liu and Wen-Ming Chen: Puzihella rosea gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from freshwater. IJSEM 67: 2383 - 2389 2017 ( DOI 10.1099/ijsem.0.001967 , PubMed 28714844 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Puzihella rosea in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF