Name: Bacillus nakamurai CCUG 68786
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140523

Type strain:

Species: Bacillus nakamurai

Culture col. no.: CCUG 68786, NRRL B-41091

NCBI tax ID(s): 1793963 (species)

SI-ID 410660

CCUG 68786

Section

Bacillus nakamurai CCUG 68786 is an obligate aerobe, spore-forming, mesophilic bacterium that has a black pigmentation and was isolated from soil sample.

Gram-positive
motile
pigmented
rod-shaped
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus nakamurai**
	Full Scientific Name (LPSN)	Bacillus nakamurai Dunlap et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43084]	Gram stain	positive

[Ref.: #43084]	Cell length	1.39-2.04 µm

[Ref.: #43084]	Cell width	0.74-0.93 µm

[Ref.: #43084]	Cell shape	rod-shaped

[Ref.: #43084]	Motility	yes

[Ref.: #43084]	Colony size	3-4 mm
[Ref.: #43084]	Colony color	creamy white
[Ref.: #43084]	Incubation period	2 days
[Ref.: #43084]	Cultivation medium used	tryptone glucose yeast extract agar (TGY)

[Ref.: #43084]	Pigment production	yes
[Ref.: #43084]	Pigmentation color	black

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43084]

Culture medium

tryptone glucose yeast extract agar (TGY)

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Culture medium growth

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Culture medium

nutritient agar (NA)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

		Temperature
[Ref.: #43084]	optimum	37 °C
[Ref.: #43084]	growth	17-50 °C
[Ref.: #63525]	growth	37 °C

[Ref.: #43084]	Temperature range	mesophilic
[Ref.: #63525]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43084]			optimum	7
[Ref.: #43084]			growth	5.5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43084]	Oxygen tolerance	obligate aerobe
[Ref.: #63525]	Oxygen tolerance	aerobe

[Ref.: #43084]	Spore description	central ellipsoidal spores with unswollen sporangia
[Ref.: #43084]	Type of spore	endospore
[Ref.: #43084]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43084]		NaCl		growth	0-9	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43084]	alpha-lactose ChEBI	-	respiration
[Ref.: #43084]	beta-d-glucose ChEBI	+	builds acid from
[Ref.: #43084]	casein	+	hydrolysis
[Ref.: #43084]	citrate ChEBI	+	assimilation
[Ref.: #43084]	D-fucose ChEBI	+	respiration
[Ref.: #43084]	D-galacturonic acid ChEBI	+	respiration
[Ref.: #43084]	D-glucuronate ChEBI	+	respiration
[Ref.: #43084]	D-saccharate ChEBI	+	respiration
[Ref.: #43084]	D-serine ChEBI	+	respiration
[Ref.: #43084]	gelatin ChEBI	+	hydrolysis
[Ref.: #43084]	L-galactonic acid gamma-lactone ChEBI	+	respiration
[Ref.: #43084]	melibiose ChEBI	-	respiration
[Ref.: #43084]	methyl beta-D-glucopyranoside ChEBI	+	respiration
[Ref.: #43084]	N-acetylgalactosamine ChEBI	+	respiration
[Ref.: #43084]	raffinose ChEBI	-	respiration
[Ref.: #43084]	stachyose ChEBI	-	respiration
[Ref.: #43084]	starch ChEBI	+	hydrolysis
[Ref.: #43084]	trehalose ChEBI	+	respiration
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43084]		catalase	+	1.11.1.6
[Ref.: #43084]		cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43084]

Fatty acid	Percentage
C15:0 anteiso	34.8
C15:0 iso	21.9
C17:0 iso	15.7
C17:0 anteiso	11.4
C16:0	9.7
C16:1ω11c	2.8
C16:0 iso	1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43084]	Sample type/isolated from	soil sample
[Ref.: #43084]	Geographic location (country and/or sea, region)	Tierra del Fuego
[Ref.: #43084]	Country	Argentina
[Ref.: #43084]	Country ISO 3 Code	ARG
[Ref.: #43084]	Continent	Middle and South America
[Ref.: #43084]	Geographic location	-54.0000°/-68.0000°

[Ref.: #63525]	Sample type/isolated from	Soil
[Ref.: #63525]	Sampling date	2004-12-02
[Ref.: #63525]	Geographic location (country and/or sea, region)	Tierra del Fuego National Park
[Ref.: #63525]	Country	Argentina
[Ref.: #63525]	Country ISO 3 Code	ARG
[Ref.: #63525]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

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Global distribution of 16S sequence KU836854 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43084]	Bacillus nakamurai strain NRRL B-41091 16S ribosomal RNA gene, partial sequence	KU836854	1508	GenBank	1793963 tax ID

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GC-content

43.8 mol%

Availability in culture collections External links

[Ref.: #43084]

Culture collection no.

CCUG 68786, NRRL B-41091

[Ref.: #96737]

SI-ID 410660

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus nakamurai sp. nov., a black-pigment-producing strain.	Dunlap CA, Saunders LP, Schisler DA, Leathers TD, Naeem N, Cohan FM, Rooney AP	Int J Syst Evol Microbiol	10.1099/ijsem.0.001135	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43084

Christopher A. Dunlap, Lauren P. Saunders, David A. Schisler, Timothy D. Leathers, Naveed Naeem, Frederick M. Cohan, Alejandro P. Rooney: Bacillus nakamurai sp. nov., a black-pigment-producing strain. IJSEM 66: 2987 - 2991 2016 ( DOI 10.1099/ijsem.0.001135 , PubMed 27150918 )

#63525 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 68786

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96737

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID410660.1 )

* These data were automatically processed and therefore are not curated

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