Name: Enterococcus crotali ETRF1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140476

Type strain:

Species: Enterococcus crotali

Strain Designation: ETRF1

Culture col. no.: LMG 28312, CCUG 65857

NCBI tax ID(s): 1453587 (species)

SI-ID 409433

CCUG 65857

Section

Enterococcus crotali ETRF1 is a facultative anaerobe, thermophilic, Gram-positive bacterium that forms circular colonies and was isolated from faecal material of the timber rattlesnake.

colony-forming
Gram-positive
facultative anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus crotali**
	Full Scientific Name (LPSN)	Enterococcus crotali McLaughlin et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43232]	Gram stain	positive

[Ref.: #43232]	Motility	no

[Ref.: #43232]	Colony size	1 mm
[Ref.: #43232]	Colony color	off-white
[Ref.: #43232]	Colony shape	circular
[Ref.: #43232]	Incubation period	1 day
[Ref.: #43232]	Cultivation medium used	Columbia agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43232]

Culture medium

Columbia agar

[Ref.: #43232]

Culture medium growth

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Culture medium

tripticase soy agar (TSA) with 5 % sheep blood

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Culture medium growth

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Culture medium

tripticase soy agar (TSA)

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Culture medium growth

		Temperature
[Ref.: #43232]	growth	45 °C
[Ref.: #43232]	growth	10 °C
[Ref.: #63256]	growth	37 °C

[Ref.: #43232]	Temperature range	thermophilic
[Ref.: #43232]	Temperature range	psychrophilic
[Ref.: #63256]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43232]	Oxygen tolerance	facultative anaerobe
[Ref.: #63256]	Oxygen tolerance	microaerophile

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43232]		NaCl		growth	6.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43232]	arabinose ChEBI	-	builds acid from
[Ref.: #43232]	arginine ChEBI	-	growth
[Ref.: #43232]	cyclodextrin ChEBI	-	builds acid from
[Ref.: #43232]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43232]	esculin ChEBI	+	growth
[Ref.: #43232]	glycerol ChEBI	+	builds acid from
[Ref.: #43232]	glycogen ChEBI	-	builds acid from
[Ref.: #43232]	hippurate ChEBI	+	growth
[Ref.: #43232]	inulin ChEBI	+	builds acid from
[Ref.: #43232]	lactose ChEBI	+	builds acid from
[Ref.: #43232]	maltose ChEBI	+	builds acid from
[Ref.: #43232]	mannitol ChEBI	+	builds acid from
[Ref.: #43232]	melezitose ChEBI	-	builds acid from
[Ref.: #43232]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #43232]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #43232]	pullulan ChEBI	-	builds acid from
[Ref.: #43232]	pyruvate ChEBI	+	growth
[Ref.: #43232]	ribose ChEBI	+	builds acid from
[Ref.: #43232]	sorbose ChEBI	+	builds acid from
[Ref.: #43232]	starch ChEBI	-	growth
[Ref.: #43232]	sucrose ChEBI	+	builds acid from
[Ref.: #43232]	tagatose ChEBI	+	builds acid from
[Ref.: #43232]	tellurite ChEBI	-	growth
[Ref.: #43232]	trehalose ChEBI	+	builds acid from
[Ref.: #43232]	urea ChEBI	-	growth
[Ref.: #43232]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43232]		acetoin ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43232]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #43232]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43232]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43232]	beta-galactosidase	-	3.2.1.23
[Ref.: #43232]	beta-glucosidase	+	3.2.1.21
[Ref.: #43232]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43232]	catalase	-	1.11.1.6
[Ref.: #43232]	glycyl tryptophan arylamidase	+
[Ref.: #43232]	leucine aminopeptidase	+	3.4.1.1
[Ref.: #43232]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43232]	pyroglutamic acid arylamidase	+
[Ref.: #43232]	tripeptide aminopeptidase	-	3.4.11.4
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43232]	Sample type/isolated from	faecal material of the timber rattlesnake
[Ref.: #43232]	Country	USA
[Ref.: #43232]	Country ISO 3 Code	USA
[Ref.: #43232]	Continent	North America
[Ref.: #43232]	Enrichment culture	sheep blood agar plate
[Ref.: #43232]	Enrichment culture composition	supplemented with 10 µg/ml rifampicin
[Ref.: #43232]	Enrichment culture duration	2 days
[Ref.: #43232]	Enrichment culture temperature	35 ̊C

[Ref.: #63256]	Sample type/isolated from	Fecal material,Timber rattlesnake
[Ref.: #63256]	Sampling date	2012-07-01
[Ref.: #63256]	Geographic location (country and/or sea, region)	Minnesota,Winona
[Ref.: #63256]	Country	USA
[Ref.: #63256]	Country ISO 3 Code	USA
[Ref.: #63256]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Reptilia	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43232]	Enterococcus sp. TRF1 16S ribosomal RNA gene, partial sequence	KF803651	1483	GenBank	1453587 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus crotali ETRF1	GCA_000633635	contig	GenBank	1453587 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus crotali ETRF1 NCBI: GCA_000633635.1
[Ref.: #69480]	motile	no	93.819	yes
[Ref.: #69480]	flagellated	no	97.267	yes
[Ref.: #69480]	gram-positive	yes	92.723	yes
[Ref.: #69480]	anaerobic	no	94.592	yes
[Ref.: #69480]	halophile	yes	76.946	no
[Ref.: #69480]	spore-forming	no	87.546	no
[Ref.: #69480]	glucose-util	yes	90.36	no
[Ref.: #69480]	aerobic	no	93.763	no
[Ref.: #69480]	thermophile	no	99.517	no
[Ref.: #69480]	glucose-ferment	yes	79.518	no

Availability in culture collections External links

[Ref.: #43232]

Culture collection no.

LMG 28312, CCUG 65857

[Ref.: #96728]

SI-ID 409433

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Enterococcus crotali sp. nov., isolated from faecal material of a timber rattlesnake.	McLaughlin RW, Shewmaker PL, Whitney AM, Humrighouse BW, Lauer AC, Loparev VN, Gulvik CA, Cochran PA, Dowd SE	Int J Syst Evol Microbiol	10.1099/ijsem.0.001900	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43232

Richard W. McLaughlin, Patricia Lynn Shewmaker, Anne M. Whitney, Ben W. Humrighouse, Ana C. Lauer, Vladimir N. Loparev, Christopher A. Gulvik, P. A. Cochran, Scot E. Dowd: Enterococcus crotali sp. nov., isolated from faecal material of a timber rattlesnake. IJSEM 67: 1984 - 1989 2017 ( DOI 10.1099/ijsem.0.001900 , PubMed 28632114 )

#63256 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 65857

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96728

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID409433.1 )

* These data were automatically processed and therefore are not curated

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