Name: Paenibacillus intestini LAH16
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140475

Type strain:

Species: Paenibacillus intestini

Strain Designation: LAH16

Culture col. no.: KCTC 33832, JCM 31621

Strain history: <- Jin-Woo Bae, Kyung Hee Univ.

NCBI tax ID(s): 1792175 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Paenibacillus intestini LAH16 is a facultative anaerobe, spore-forming, Gram-negative bacterium that forms circular colonies and was isolated from hindgut of the honey bee A. mellifera.

colony-forming
Gram-negative
motile
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus intestini**
	Full Scientific Name (LPSN)	Paenibacillus intestini Yun et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43251]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #43251]	Cell length	1-4 µm

[Ref.: #43251]	Cell width	0.5-0.6 µm

[Ref.: #43251]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43251]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #43251]	Colony color	greyish
[Ref.: #43251]	Colony shape	circular
[Ref.: #43251]	Cultivation medium used	Columbia Agar containing 5 % sheep blood (CBA; BD)

[Ref.: #43251]	Colony color	yellowish-white
[Ref.: #43251]	Colony shape	circular
[Ref.: #43251]	Cultivation medium used	Luria-Bertani Agar (LA; BD)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43251]

Culture medium

CBA

[Ref.: #43251]

Culture medium growth

[Ref.: #43251]

Culture medium

[Ref.: #43251]

Culture medium growth

		Temperature
[Ref.: #43251]	optimum	20-37 °C
[Ref.: #43251]	growth	10-37 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30-37 °C

	pH	Kind of pH		pH
[Ref.: #43251]			optimum	6.0-8.0
[Ref.: #43251]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43251]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe

[Ref.: #43251]	Type of spore	endospore
[Ref.: #43251]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43251]		NaCl		growth	0-6	%
[Ref.: #43251]		NaCl		optimum	0-3	%

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43251]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43251]	amygdalin ChEBI	+	builds acid from
[Ref.: #43251]	arbutin ChEBI	+	builds acid from
[Ref.: #43251]	cellobiose ChEBI	+	builds acid from
[Ref.: #43251]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43251]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43251]	D-fructose ChEBI	+	builds acid from
[Ref.: #43251]	D-fucose ChEBI	-	builds acid from
[Ref.: #43251]	D-galactose ChEBI	+	builds acid from
[Ref.: #43251]	D-glucose ChEBI	+	builds acid from
[Ref.: #43251]	D-lyxose ChEBI	-	builds acid from
[Ref.: #43251]	D-mannitol ChEBI	+	builds acid from
[Ref.: #43251]	D-mannose ChEBI	+	builds acid from
[Ref.: #43251]	D-ribose ChEBI	+	builds acid from
[Ref.: #43251]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #43251]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43251]	D-xylose ChEBI	+	builds acid from
[Ref.: #43251]	esculin ChEBI	+	builds acid from
[Ref.: #43251]	gentiobiose ChEBI	+	builds acid from
[Ref.: #43251]	gluconate ChEBI	+	builds acid from
[Ref.: #43251]	glycerol ChEBI	+	builds acid from
[Ref.: #43251]	glycogen ChEBI	+	builds acid from
[Ref.: #43251]	inulin ChEBI	-	builds acid from
[Ref.: #43251]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43251]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43251]	L-fucose ChEBI	+	builds acid from
[Ref.: #43251]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43251]	L-xylose ChEBI	-	builds acid from
[Ref.: #43251]	lactose ChEBI	+	builds acid from
[Ref.: #43251]	maltose ChEBI	+	builds acid from
[Ref.: #43251]	melezitose ChEBI	+	builds acid from
[Ref.: #43251]	melibiose ChEBI	+	builds acid from
[Ref.: #43251]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #43251]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43251]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #43251]	myo-inositol ChEBI	+	builds acid from
[Ref.: #43251]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #43251]	raffinose ChEBI	+	builds acid from
[Ref.: #43251]	salicin ChEBI	+	builds acid from
[Ref.: #43251]	starch ChEBI	+	builds acid from
[Ref.: #43251]	sucrose ChEBI	+	builds acid from
[Ref.: #43251]	trehalose ChEBI	+	builds acid from
[Ref.: #43251]	turanose ChEBI	+	builds acid from
[Ref.: #43251]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43251]	acid phosphatase	+	3.1.3.2
[Ref.: #43251]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43251]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43251]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43251]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43251]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43251]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43251]	beta-galactosidase	+	3.2.1.23
[Ref.: #43251]	beta-glucosidase	+	3.2.1.21
[Ref.: #43251]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43251]	catalase	+	1.11.1.6
[Ref.: #43251]	cystine arylamidase	-	3.4.11.3
[Ref.: #43251]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43251]	esterase (C 4)	+
[Ref.: #43251]	esterase Lipase (C 8)	+
[Ref.: #43251]	leucine arylamidase	-	3.4.11.1
[Ref.: #43251]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43251]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43251]	trypsin	-	3.4.21.4
[Ref.: #43251]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43251]

Fatty acid	Percentage
C15:0 anteiso	46.4
C16:0 iso	18.1
C16:0	11.6
C17:0 anteiso	7.4
C14:0	3.9
C16:1ω11c	2.9
C15:0 iso	2.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43251]	Sample type/isolated from	hindgut of the honey bee A. mellifera
[Ref.: #43251]	Country	Republic of Korea
[Ref.: #43251]	Country ISO 3 Code	KOR
[Ref.: #43251]	Continent	Asia
[Ref.: #43251]	Enrichment culture	Luria-Bertani Agar (LA; BD)
[Ref.: #43251]	Enrichment culture duration	14 days
[Ref.: #43251]	Enrichment culture temperature	34 ̊C
[Ref.: #43251]	Isolation/enrichment procedure	standard dilution-plating method

[Ref.: #67770]	Sample type/isolated from	Hindgut of the honey bee (Apis mellifera)
[Ref.: #67770]	Host species	Apis mellifera
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From `Apis mellifera` hindgut
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU573975 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	18
Soil Samples	93
Animal Samples	40
Plant Samples	728
Total Samples	879

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43251]	Paenibacillus intestini strain LAH16 16S ribosomal RNA gene, partial sequence	KU573975	1453	GenBank ENA	1792175 tax ID

[Ref.: #43251]	GC-content	46.1 mol%	Thermal denaturation, fluorometry
[Ref.: #67771]	GC-content	46.1 mol%	fluorimetric
[Ref.: #67770]	GC-content	46.1 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #43251]

Culture collection no.

KCTC 33832, JCM 31621

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus apis sp. nov. and Paenibacillus intestini sp. nov., isolated from the intestine of the honey bee Apis mellifera.	Yun JH, Lee JY, Kim PS, Jung MJ, Bae JW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001887	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43251

Ji-Hyun Yun, June-Young Lee, Pil Soo Kim, Mi-Ja Jung, Jin-Woo Bae: Paenibacillus apis sp. nov. and Paenibacillus intestini sp. nov., isolated from the intestine of the honey bee Apis mellifera. IJSEM 67: 1918 - 1924 2017 ( DOI 10.1099/ijsem.0.001887 , PubMed 28613144 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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