Name: Desulfosporosinus nitroreducens 59.4B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140296

Type strain:

Species: Desulfosporosinus nitroreducens

Strain Designation: 59.4B

Culture col. no.: DSM 101608, JCM 31105

Strain history: V. Vandieken; Univ. of Oldenburg, Germany; 59.4B.

NCBI tax ID(s): 2018668 (species)

SI-ID 404424

DSM 101608

Other strains from Desulfosporosinus nitroreducens

D. nitroreducens 59.4F, DSM 101562, JCM 31104

Section

Desulfosporosinus nitroreducens 59.4B is an anaerobe, spore-forming, mesophilic bacterium that was isolated from marine subsurface sediment.

Gram-positive
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Desulfitobacteriaceae*
	Genus	*Desulfosporosinus*
	Species	**Desulfosporosinus nitroreducens**
	Full Scientific Name (LPSN)	Desulfosporosinus nitroreducens Vandieken et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43225]	Gram stain	positive

[Ref.: #43225]	Cell length	2.6-5 µm

[Ref.: #43225]	Cell width	0.7-1.1 µm

[Ref.: #43225]	Cell shape	rod-shaped

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42903]

Culture medium

DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)

[Ref.: #42903]

Culture medium growth

[Ref.: #42903]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42903]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42903]	growth	25 °C
[Ref.: #43225]	optimum	25-30 °C
[Ref.: #43225]	growth	4-30 °C
[Ref.: #67770]	growth	25 °C

[Ref.: #42903]	Temperature range	mesophilic
[Ref.: #43225]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43225]			optimum	7.7-7.8
[Ref.: #43225]			growth	6.4-8.1

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43225]

Oxygen tolerance

anaerobe

[Ref.: #43225]	Type of spore	endospore
[Ref.: #43225]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43225]		NaCl		growth	0-1	%
[Ref.: #43225]		NaCl		optimum	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43225]	1,2-butandiol ChEBI	-	electron acceptor
[Ref.: #43225]	acetate ChEBI	-	electron acceptor
[Ref.: #43225]	alanine ChEBI	-	electron acceptor
[Ref.: #43225]	arabinose ChEBI	-	electron acceptor
[Ref.: #43225]	arginine ChEBI	-	electron acceptor
[Ref.: #43225]	asparagine ChEBI	-	electron acceptor
[Ref.: #43225]	betaine ChEBI	+	anaerobic growth
[Ref.: #43225]	butyrate ChEBI	-	electron acceptor
[Ref.: #43225]	cellobiose ChEBI	-	electron acceptor
[Ref.: #43225]	dimethyl sulfoxide ChEBI	+	electron acceptor
[Ref.: #43225]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #43225]	ethanol ChEBI	+	anaerobic growth
[Ref.: #43225]	fumarate ChEBI	+	electron acceptor
[Ref.: #43225]	galactose ChEBI	-	electron acceptor
[Ref.: #43225]	glucose ChEBI	-	electron acceptor
[Ref.: #43225]	glutamine ChEBI	-	electron acceptor
[Ref.: #43225]	glycerol ChEBI	+	anaerobic growth
[Ref.: #43225]	histidine ChEBI	-	electron acceptor
[Ref.: #43225]	isoleucine ChEBI	-	electron acceptor
[Ref.: #43225]	lactate ChEBI	+	anaerobic growth
[Ref.: #43225]	lactose ChEBI	-	electron acceptor
[Ref.: #43225]	lysine ChEBI	-	electron acceptor
[Ref.: #43225]	malate ChEBI	+	electron acceptor
[Ref.: #43225]	malate ChEBI	+	anaerobic growth
[Ref.: #43225]	maltose ChEBI	-	electron acceptor
[Ref.: #43225]	N-acetylglucosamine ChEBI	-	electron acceptor
[Ref.: #43225]	proline ChEBI	-	electron acceptor
[Ref.: #43225]	propanol ChEBI	-	electron acceptor
[Ref.: #43225]	propionate ChEBI	-	electron acceptor
[Ref.: #43225]	pyruvate ChEBI	+	anaerobic growth
[Ref.: #43225]	rhamnose ChEBI	-	electron acceptor
[Ref.: #43225]	ribose ChEBI	-	electron acceptor
[Ref.: #43225]	succinate ChEBI	-	electron acceptor
[Ref.: #43225]	sulfate ChEBI	+	electron acceptor
[Ref.: #43225]	sulfite ChEBI	+	electron acceptor
[Ref.: #43225]	thiosulfate ChEBI	+	electron acceptor
[Ref.: #43225]	xylose ChEBI	-	electron acceptor
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43225]		catalase	-	1.11.1.6
[Ref.: #43225]		cytochrome oxidase	+	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43225]

Fatty acid	Percentage
C16:1ω7c	25.3
C18:1ω9c	20.9
C16:0	15.2
C18:0	9.2
C16:1ω9c	8.1
C14:0	4.5
C18:1ω7c	3.9
C15:1ω8c	3.2
C17:1ω6	2.1
C18:2	2
C15:1ω4	1.2
C16:1ω5c	1
C15:0	0.6
C18:1ω5c	0.5
C17:0	0.5
C15:0 anteiso	0.3
C15:1ω6c	0.3
C15:0 iso	0.2
C17:1ω8	0.2
C17:0 iso	0.2
C16:1ω7t	0.2
C16:0 iso	0.1
12-methyl C15:1ω7c	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42903]	Sample type/isolated from	marine subsurface sediment
[Ref.: #42903]	Geographic location (country and/or sea, region)	Baltic Sea, Little Belt (IODP Expedition 347, Research vessel "Greatship Manisha"); depth of 12.7 m at station M0059
[Ref.: #42903]	Country	Denmark
[Ref.: #42903]	Country ISO 3 Code	DNK
[Ref.: #42903]	Continent	Europe
[Ref.: #42903]	Geographic location	55.0048°/10.1082°

[Ref.: #43225]	Sample type/isolated from	sediment samples from 12.7 m depth
[Ref.: #43225]	Sampling date	2013-10-30
[Ref.: #43225]	Geographic location (country and/or sea, region)	Little Belt, Baltic Sea
[Ref.: #43225]	Country	Denmark
[Ref.: #43225]	Country ISO 3 Code	DNK
[Ref.: #43225]	Continent	Europe
[Ref.: #43225]	Geographic location	55.0000°/10.0000°
[Ref.: #43225]	Enrichment culture	brackish water medium
[Ref.: #43225]	Enrichment culture temperature	15 ̊C
[Ref.: #43225]	Isolation/enrichment procedure	several transfers of anoxic deep-agar dilution series

[Ref.: #67770]	Sample type/isolated from	Subsurface of marine sediment from Little Belt (Baltic Sea)
[Ref.: #67770]	Country	Denmark
[Ref.: #67770]	Country ISO 3 Code	DNK
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42903]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42903]	Desulfosporosinus nitroreducens strain 59.4B 16S ribosomal RNA gene, partial sequence	KX822013	1448	ENA	2018668 tax ID

[Ref.: #43225]	GC-content	42.1-42.6 mol%
[Ref.: #42903]	GC-content	42 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	42.6 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #42903]

Culture collection no.

DSM 101608, JCM 31105

[Ref.: #96601]

SI-ID 404424

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfosporosinus metallidurans sp. nov., an acidophilic, metal-resistant sulfate-reducing bacterium from acid mine drainage.	Panova IA, Ikkert O, Avakyan MR, Kopitsyn DS, Mardanov AV, Pimenov NV, Shcherbakova VA, Ravin NV, Karnachuk OV	Int J Syst Evol Microbiol	10.1099/ijsem.0.004876	2021	*
Phylogeny	Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea.	Vandieken V, Niemann H, Engelen B, Cypionka H	Int J Syst Evol Microbiol	10.1099/ijsem.0.001883	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42903

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101608

#43225

Verona Vandieken, Helge Niemann, Bert Engelen, Heribert Cypionka: Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. And Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea. IJSEM 67: 1887 - 1893 2017 ( DOI 10.1099/ijsem.0.001883 , PubMed 28646634 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#96601

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404424.1 )

* These data were automatically processed and therefore are not curated

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