Name: Desulfosporosinus fructosivorans 63.6F
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140297

Type strain:

Species: Desulfosporosinus fructosivorans

Strain Designation: 63.6F

Culture col. no.: DSM 101609, JCM 31106

Strain history: V. Vandieken; Univ. of Oldenburg, Germany; 63.6F.

NCBI tax ID(s): 2018669 (species)

SI-ID 399302

DSM 101609

Section

Desulfosporosinus fructosivorans 63.6F is an anaerobe, spore-forming, psychrophilic bacterium that was isolated from marine subsurface sediment.

Gram-positive
rod-shaped
spore-forming
anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Desulfitobacteriaceae*
	Genus	*Desulfosporosinus*
	Species	**Desulfosporosinus fructosivorans**
	Full Scientific Name (LPSN)	Desulfosporosinus fructosivorans Vandieken et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43225]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #43225]	Cell length	2.1-4.5 µm

[Ref.: #43225]	Cell width	0.9 µm

[Ref.: #43225]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	yes Confidence in %93.009

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42904]

Culture medium

DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)

[Ref.: #42904]

Culture medium growth

[Ref.: #42904]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42904]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42904]	growth	18 °C
[Ref.: #43225]	optimum	20 °C
[Ref.: #43225]	growth	10-20 °C
[Ref.: #67770]	growth	15 °C

[Ref.: #42904]	Temperature range	psychrophilic
[Ref.: #43225]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #43225]			optimum	7.5-8.0
[Ref.: #43225]			growth	6.5-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43225]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %98.651

[Ref.: #43225]	Type of spore	endospore
[Ref.: #43225]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.619

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43225]		NaCl		growth	0-3.5	%
[Ref.: #43225]		NaCl		optimum	0-0.1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43225]	betaine ChEBI	+	electron donor
[Ref.: #43225]	dihydrogen ChEBI	+	electron donor
[Ref.: #43225]	dimethyl sulfoxide ChEBI	+	electron acceptor
[Ref.: #43225]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #43225]	ethanol ChEBI	+	electron donor
[Ref.: #43225]	fructose ChEBI	+	electron donor
[Ref.: #43225]	fructose ChEBI	+	fermentation
[Ref.: #43225]	fumarate ChEBI	+	electron acceptor
[Ref.: #43225]	glycerol ChEBI	+	electron donor
[Ref.: #43225]	glycerol ChEBI	+	fermentation
[Ref.: #43225]	lactate ChEBI	+	electron donor
[Ref.: #43225]	malate ChEBI	+	electron acceptor
[Ref.: #43225]	malate ChEBI	+	electron donor
[Ref.: #43225]	malate ChEBI	+	fermentation
[Ref.: #43225]	mannitol ChEBI	+	electron donor
[Ref.: #43225]	pyruvate ChEBI	+	fermentation
[Ref.: #43225]	serine ChEBI	+	electron donor
[Ref.: #43225]	sulfate ChEBI	+	electron acceptor
[Ref.: #43225]	sulfite ChEBI	+	electron acceptor
[Ref.: #43225]	thiosulfate ChEBI	+	electron acceptor
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43225]		catalase	-	1.11.1.6
[Ref.: #43225]		cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43225]

Fatty acid	Percentage
C18:1ω9c	25.2
C16:0	22
C18:0	16.9
C16:1ω7c	12.6
C18:2	3.8
C14:0	3.8
C16:1ω9c	3.2
C18:1ω7c	2
C15:0	1.3
C15:1ω4	1.3
C17:1ω8	1.1
C18:1ω5c	1.1
C17:0	0.9
C15:0 anteiso	0.8
C16:1ω5c	0.7
C15:1ω6c	0.6
C17:0 iso	0.5
C17:1ω6	0.4
C15:1ω8c	0.4
C15:0 iso	0.4
C16:1ω7t	0.3
C16:0 iso	0.2
12-methyl C15:1ω7c	0.2

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	threonine metabolism	100	10 of 10
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	butanoate fermentation	100	4 of 4
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	propanol degradation	100	7 of 7
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	isoleucine metabolism	100	8 of 8
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	glutamate and glutamine metabolism	96.43	27 of 28
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	glycolysis	88.24	15 of 17
[Ref.: #66794]	pyrimidine metabolism	86.67	39 of 45
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	citric acid cycle	85.71	12 of 14
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	83.33	10 of 12
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
[Ref.: #66794]	purine metabolism	82.98	78 of 94
[Ref.: #66794]	alanine metabolism	82.76	24 of 29
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	metabolism of amino sugars and derivatives	80	4 of 5
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	ethylmalonyl-CoA pathway	80	4 of 5
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	vitamin B12 metabolism	79.41	27 of 34
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	leucine metabolism	76.92	10 of 13
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	methionine metabolism	73.08	19 of 26
[Ref.: #66794]	glutathione metabolism	71.43	10 of 14
[Ref.: #66794]	tryptophan metabolism	71.05	27 of 38
[Ref.: #66794]	sulfate reduction	69.23	9 of 13
[Ref.: #66794]	oxidative phosphorylation	69.23	63 of 91
[Ref.: #66794]	histidine metabolism	68.97	20 of 29
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	lipid metabolism	67.74	21 of 31
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	arginine metabolism	66.67	16 of 24
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	IAA biosynthesis	66.67	2 of 3
[Ref.: #66794]	phenol degradation	65	13 of 20
[Ref.: #66794]	lysine metabolism	64.29	27 of 42
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	61.54	8 of 13
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	polyamine pathway	60.87	14 of 23
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	coenzyme M biosynthesis	60	6 of 10
[Ref.: #66794]	3-chlorocatechol degradation	60	3 of 5
[Ref.: #66794]	4-hydroxyphenylacetate degradation	60	6 of 10
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	nitrate assimilation	55.56	5 of 9
[Ref.: #66794]	metabolism of disaccharids	54.55	6 of 11
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	phenylpropanoid biosynthesis	46.15	6 of 13
[Ref.: #66794]	allantoin degradation	44.44	4 of 9
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	benzoyl-CoA degradation	42.86	3 of 7
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	creatinine degradation	40	2 of 5
[Ref.: #66794]	bacilysin biosynthesis	40	2 of 5
[Ref.: #66794]	3-phenylpropionate degradation	40	6 of 15
[Ref.: #66794]	phenylacetate degradation (aerobic)	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	degradation of pentoses	39.29	11 of 28
[Ref.: #66794]	androgen and estrogen metabolism	37.5	6 of 16
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	degradation of sugar acids	36	9 of 25
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	4-hydroxymandelate degradation	33.33	3 of 9
[Ref.: #66794]	methanogenesis from CO2	33.33	4 of 12
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	cholesterol biosynthesis	27.27	3 of 11
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42904]	Sample type/isolated from	marine subsurface sediment
[Ref.: #42904]	Geographic location (country and/or sea, region)	Baltic Sea, Landsort Deep (IODP Expedition 347, Research vessel "Greatship Manisha"); sediment depth of 12.2 m at station M0063
[Ref.: #42904]	Country	Sweden
[Ref.: #42904]	Country ISO 3 Code	SWE
[Ref.: #42904]	Continent	Europe
[Ref.: #42904]	Geographic location	58.6223°/18.2542°

[Ref.: #43225]	Sample type/isolated from	sediment samples from 12.2 m depth
[Ref.: #43225]	Sampling date	2013-10-17
[Ref.: #43225]	Geographic location (country and/or sea, region)	Landsort Deep, Baltic Sea
[Ref.: #43225]	Country	Sweden
[Ref.: #43225]	Country ISO 3 Code	SWE
[Ref.: #43225]	Continent	Europe
[Ref.: #43225]	Geographic location	58.0000°/18.0000°
[Ref.: #43225]	Enrichment culture	freshwater medium
[Ref.: #43225]	Enrichment culture temperature	15 ̊C
[Ref.: #43225]	Isolation/enrichment procedure	several transfers of anoxic deep-agar dilution series

[Ref.: #67770]	Sample type/isolated from	Subsurface of marine sediment from Little Belt (Baltic Sea)
[Ref.: #67770]	Country	Denmark
[Ref.: #67770]	Country ISO 3 Code	DNK
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42904]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42904]	Desulfosporosinus fructosivorans strain 63.6F 16S ribosomal RNA gene, partial sequence	KX822015	1529	ENA	2018669 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Desulfosporosinus fructosivorans 63.6F	GCA_004766045	scaffold	GenBank	2018669 tax ID

[Ref.: #43225]	GC-content	43.2 mol%
[Ref.: #42904]	GC-content	43.2 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Desulfosporosinus fructosivorans 63.6F NCBI: GCA_004766045.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Desulfosporosinus fructosivorans 63.6F NCBI: GCA_004766045.1
[Ref.: #69480]	motile	yes	82.919	no
[Ref.: #69480]	flagellated	no	62.544	no
[Ref.: #69480]	gram-positive	no	56.163	yes
[Ref.: #69480]	anaerobic	yes	96.543	yes
[Ref.: #69480]	aerobic	no	95.947	yes
[Ref.: #69480]	halophile	no	96.255	no
[Ref.: #69480]	spore-forming	yes	94.136	no
[Ref.: #69480]	glucose-util	yes	81.265	no
[Ref.: #69480]	thermophile	no	98.885	yes
[Ref.: #69480]	glucose-ferment	no	75.836	no

Availability in culture collections External links

[Ref.: #42904]

Culture collection no.

DSM 101609, JCM 31106

[Ref.: #96602]

SI-ID 399302

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea.	Vandieken V, Niemann H, Engelen B, Cypionka H	Int J Syst Evol Microbiol	10.1099/ijsem.0.001883	2017	*
Genetics	Draft Genome Sequence of Desulfosporosinus fructosivorans Strain 63.6F(T), Isolated from Marine Sediment in the Baltic Sea.	Hausmann B, Vandieken V, Pjevac P, Schreck K, Herbold CW, Loy A	Microbiol Resour Announc	10.1128/MRA.00427-19	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42904

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101609

#43225

Verona Vandieken, Helge Niemann, Bert Engelen, Heribert Cypionka: Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. And Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea. IJSEM 67: 1887 - 1893 2017 ( DOI 10.1099/ijsem.0.001883 , PubMed 28646634 )

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96602

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399302.1 )

* These data were automatically processed and therefore are not curated

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