Name: Alkalicoccus halolimnae BZ-SZ-XJ29
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140264

Type strain:

Species: Alkalicoccus halolimnae

Strain Designation: BZ-SZ-XJ29

Culture col. no.: DSM 29191, CGMCC 1.12936, JCM 30193

Strain history: B. Zhao; Grad. Sch. of Chin. Acad. of Agric. Sci., China; BZ-SZ-XJ29.

NCBI tax ID(s): 1667239 (species)

SI-ID 401440

DSM 29191

Other strains from Alkalicoccus halolimnae

A. halolimnae BZ-SZ-Xj39, DSM 29242

Section

Alkalicoccus halolimnae BZ-SZ-XJ29 is an aerobe, moderately halophilic, mesophilic bacterium that forms circular colonies and was isolated from combined water and sediment sample.

coccus-shaped
colony-forming
Gram-positive
aerobe
mesophilic
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Alkalicoccus*
	Species	**Alkalicoccus halolimnae**
	Full Scientific Name (LPSN)	Alkalicoccus halolimnae Zhao et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43205]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43205]	Cell length	1.2-1.5 µm

[Ref.: #43205]	Cell width	1.2-1.5 µm

[Ref.: #43205]	Cell shape	coccus-shaped

[Ref.: #43205]	Motility	no

[Ref.: #43205]	Colony size	1-2 mm
[Ref.: #43205]	Colony color	yellow
[Ref.: #43205]	Colony shape	circular
[Ref.: #43205]	Incubation period	3 days
[Ref.: #43205]	Cultivation medium used	modified solid medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43205]

Culture medium

modified solid medium

[Ref.: #43205]

Culture medium growth

[Ref.: #43205]

Culture medium composition

expand / minimize

[Ref.: #42871]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #42871]

Culture medium growth

[Ref.: #42871]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42871]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42871]	growth	35 °C
[Ref.: #43205]	optimum	33 °C
[Ref.: #43205]	growth	5-41 °C
[Ref.: #67770]	growth	35 °C

[Ref.: #42871]	Temperature range	mesophilic
[Ref.: #43205]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43205]			optimum	7.5
[Ref.: #43205]			growth	6.0-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43205]

Oxygen tolerance

aerobe

[Ref.: #43205]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.504

[Ref.: #43205]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43205]		NaCl		growth	4.3-24.3	%(w/v)
[Ref.: #43205]		NaCl		optimum	8.3-12.3	%(w/v)

[Ref.: #43205]	Observation	accumaltion of polyhydroxyalkanoate granules
[Ref.: #43205]	Observation	production of exopolysaccharide
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43205]	acetoin ChEBI	+	hydrolysis
[Ref.: #43205]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #43205]	alpha-D-glucose ChEBI	+	builds acid from
[Ref.: #43205]	casein	+	hydrolysis
[Ref.: #43205]	cellobiose ChEBI	-	carbon source
[Ref.: #43205]	D-fructose ChEBI	+	carbon source
[Ref.: #43205]	D-fructose ChEBI	+	builds acid from
[Ref.: #43205]	D-fucose ChEBI	+	carbon source
[Ref.: #43205]	D-fucose ChEBI	+	builds acid from
[Ref.: #43205]	D-galactose ChEBI	+	carbon source
[Ref.: #43205]	D-galactose ChEBI	-	carbon source
[Ref.: #43205]	D-galactose ChEBI	-	builds acid from
[Ref.: #43205]	D-mannose ChEBI	+	carbon source
[Ref.: #43205]	D-mannose ChEBI	+	builds acid from
[Ref.: #43205]	D-ribose ChEBI	+	carbon source
[Ref.: #43205]	D-ribose ChEBI	+	builds acid from
[Ref.: #43205]	D-serine ChEBI	-	nitrogen source
[Ref.: #43205]	D-xylose ChEBI	+	carbon source
[Ref.: #43205]	D-xylose ChEBI	+	builds acid from
[Ref.: #43205]	gamma-aminobutyric acid ChEBI	+	nitrogen source
[Ref.: #43205]	gamma-aminobutyric acid ChEBI	+	carbon source
[Ref.: #43205]	glucose ChEBI	-	fermentation
[Ref.: #43205]	glucuronamide ChEBI	-	nitrogen source
[Ref.: #43205]	glycine-proline ChEBI	-	nitrogen source
[Ref.: #43205]	hippurate ChEBI	-	hydrolysis
[Ref.: #43205]	L-alanine ChEBI	-	nitrogen source
[Ref.: #43205]	L-arabinose ChEBI	+	carbon source
[Ref.: #43205]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43205]	L-arginine ChEBI	-	nitrogen source
[Ref.: #43205]	L-aspartate ChEBI	-	nitrogen source
[Ref.: #43205]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #43205]	L-histidine ChEBI	-	nitrogen source
[Ref.: #43205]	L-pyroglutamic acid ChEBI	-	nitrogen source
[Ref.: #43205]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43205]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43205]	L-serine ChEBI	-	nitrogen source
[Ref.: #43205]	maltose ChEBI	+	carbon source
[Ref.: #43205]	maltose ChEBI	+	builds acid from
[Ref.: #43205]	melezitose ChEBI	+	carbon source
[Ref.: #43205]	melezitose ChEBI	+	builds acid from
[Ref.: #43205]	N-acetylglucosamine ChEBI	+	nitrogen source
[Ref.: #43205]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #43205]	nitrate ChEBI	-	reduction
[Ref.: #43205]	nitrite ChEBI	-	reduction
[Ref.: #43205]	raffinose ChEBI	-	carbon source
[Ref.: #43205]	stachyose ChEBI	-	carbon source
[Ref.: #43205]	starch ChEBI	+	hydrolysis
[Ref.: #43205]	sucrose ChEBI	+	carbon source
[Ref.: #43205]	sucrose ChEBI	+	builds acid from
[Ref.: #43205]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43205]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43205]	tyrosine ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43205]	amoxicillin ChEBI	yes	10	µg (disc)
[Ref.: #43205]	ampicillin ChEBI	yes	50	µg (disc)
[Ref.: #43205]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #43205]	cefotaxime ChEBI	yes	30	µg (disc)
[Ref.: #43205]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #43205]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43205]	clarithromycin ChEBI	yes	15	µg (disc)
[Ref.: #43205]	clindamycin ChEBI	yes	2	µg (disc)
[Ref.: #43205]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #43205]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #43205]	kanamycin ChEBI				yes	50	µg (disc)
[Ref.: #43205]	nalidixic acid ChEBI				yes	30	µg (disc)
[Ref.: #43205]	neomycin ChEBI				yes	30	µg (disc)
[Ref.: #43205]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #43205]	rifampicin ChEBI	yes	30	µg (disc)
[Ref.: #43205]	streptomycin ChEBI				yes	300	µg (disc)
[Ref.: #43205]	sulfamethoxazole ChEBI	yes	300	µg (disc)
[Ref.: #43205]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43205]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43205]	acetoin ChEBI	yes
[Ref.: #43205]	hydrogen sulfide ChEBI	no
[Ref.: #43205]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #43205]		acetoin ChEBI	+
[Ref.: #43205]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #43205]	arginine decarboxylase	-	4.1.1.19
[Ref.: #43205]	catalase	+	1.11.1.6
[Ref.: #43205]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43205]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43205]	ornithine decarboxylase	-	4.1.1.17

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43205]	Sample type/isolated from	combined water and sediment sample
[Ref.: #43205]	Geographic location (country and/or sea, region)	salt lake near Urumqi city, Xinjiang Uyghur Autonomous Region of China
[Ref.: #43205]	Country	China
[Ref.: #43205]	Country ISO 3 Code	CHN
[Ref.: #43205]	Continent	Asia
[Ref.: #43205]	Geographic location	43.0000°/88.0000°
[Ref.: #43205]	Enrichment culture	modified solid medium
[Ref.: #43205]	Enrichment culture duration	5 days
[Ref.: #43205]	Enrichment culture temperature	37 ̊C
[Ref.: #43205]	Isolation/enrichment procedure	10-fold dilution-plating technique

[Ref.: #42871]	Sample type/isolated from	mixture of water and sediment of a saline-alkaline lake
[Ref.: #42871]	Geographic location (country and/or sea, region)	Xinjiang, close to Urumqi city, salt lake near the National Road No. 314 (43° 24' 35'' N 88° 6' 39'' E, 1072 m elevation)
[Ref.: #42871]	Country	China
[Ref.: #42871]	Country ISO 3 Code	CHN
[Ref.: #42871]	Continent	Asia
[Ref.: #42871]	Geographic location	43.4097°/88.1108°

[Ref.: #67770]	Sample type/isolated from	Salt lake
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42871]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42871]	Bacillus sp. strain BZ-SZ-XJ29 16S ribosomal RNA gene, partial sequence	KX618877	1538	ENA	1409 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Alkalicoccus halolimnae BZ-SZ-XJ29	GCA_008014775	scaffold	GenBank	1667239 tax ID	*

[Ref.: #42871]	GC-content	40.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	40 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalicoccus halolimnae BZ-SZ-XJ29 NCBI: GCA_008014775.1
[Ref.: #69481]	spore-forming	no	84	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkalicoccus halolimnae BZ-SZ-XJ29 NCBI: GCA_008014775.1
[Ref.: #69480]	motile	no	86.111	yes
[Ref.: #69480]	flagellated	no	87.233	yes
[Ref.: #69480]	gram-positive	yes	90.759	yes
[Ref.: #69480]	anaerobic	no	98.812	no
[Ref.: #69480]	aerobic	yes	92.377	yes
[Ref.: #69480]	halophile	yes	94.019	yes
[Ref.: #69480]	spore-forming	no	77.223	yes
[Ref.: #69480]	thermophile	no	97.367	no
[Ref.: #69480]	glucose-util	yes	92.989	no
[Ref.: #69480]	glucose-ferment	no	86.492	no

Availability in culture collections External links

[Ref.: #42871]

Culture collection no.

DSM 29191, CGMCC 1.12936, JCM 30193

[Ref.: #96570]

SI-ID 401440

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Bacillus saliphilus as Alkalicoccus saliphilus gen. nov., comb. nov., and description of Alkalicoccus halolimnae sp. nov., a moderately halophilic bacterium isolated from a salt lake.	Zhao B, Lu W, Zhang S, Liu K, Yan Y, Li J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001759	2017	*
Genetics	Draft Genome Sequence of Alkalicoccus halolimnae BZ-SZ-XJ29(T), a Moderately Halophilic Bacterium Isolated from a Salt Lake.	Zhang Y, Qiu D, Liao Z, Zhao B	Microbiol Resour Announc	10.1128/MRA.00500-20	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42871

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29191

#43205

Baisuo Zhao, Weidong Lu, Shanshan Zhang, Kang Liu, Yanchun Yan and Jun Li: Reclassification of Bacillus saliphilus as Alkalicoccus saliphilus gen. nov., comb. nov., and description of Alkalicoccus halolimnae sp. nov., a moderately halophilic bacterium isolated from a salt lake. IJSEM 67: 1557 - 1563 2017 ( DOI 10.1099/ijsem.0.001759 , PubMed 28032538 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96570

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401440.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Alkalicoccus halolimnae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF