Name: Azospirillum rugosum DSM 19657
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13998

Type strain:

Species: Azospirillum rugosum

Culture col. no.: DSM 19657, CCUG 53966, IMMIB AFH-6

Strain history: <- IMMIB <- A. B. Arun, Dept. Soil and Environmental Sci., Natl. Chung Hsing Univ., Taichung, Taiwan; IMMIB AFH-6

NCBI tax ID(s): 416170 (species)

SI-ID 398830

DSM 19657, CCUG 53966

Section

Azospirillum rugosum DSM 19657 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from oil-contaminated soil near the oil refinery located in Kaohsiung county.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Azospirillum*
	Species	**Azospirillum rugosum**
	Full Scientific Name (LPSN)	Azospirillum rugosum Young et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32167]	Gram stain	negative

[Ref.: #32167]	Cell shape	rod-shaped

[Ref.: #32167]	Motility	yes

[Ref.: #32167]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8235]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #8235]

Culture medium growth

[Ref.: #8235]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8235]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8235]	growth	28 °C
[Ref.: #32167]	growth	22-37 °C
[Ref.: #60090]	growth	35 °C

[Ref.: #8235]	Temperature range	mesophilic
[Ref.: #60090]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32167]	Oxygen tolerance	aerobe
[Ref.: #60090]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32167]		NaCl		growth	2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32167]	2-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #32167]	adipate ChEBI	+	carbon source
[Ref.: #32167]	esculin ChEBI	+	hydrolysis
[Ref.: #32167]	fructose ChEBI	+	carbon source
[Ref.: #32167]	glycerol ChEBI	+	carbon source
[Ref.: #32167]	malate ChEBI	+	carbon source
[Ref.: #32167]	nitrate ChEBI	+	reduction
[Ref.: #32167]	xylose ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #32167]	alkaline phosphatase	+	3.1.3.1
[Ref.: #32167]	alpha-galactosidase	+	3.2.1.22
[Ref.: #32167]	catalase	+	1.11.1.6
[Ref.: #32167]	cytochrome oxidase	+	1.9.3.1
[Ref.: #32167]	gelatinase	+
[Ref.: #32167]	pyrazinamidase	+	3.5.1.B15
[Ref.: #32167]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8235]	Sample type/isolated from	oil-contaminated soil near the oil refinery located in Kaohsiung county
[Ref.: #8235]	Country	Taiwan
[Ref.: #8235]	Country ISO 3 Code	TWN
[Ref.: #8235]	Continent	Asia

[Ref.: #60090]	Sample type/isolated from	Oil contaminated soil
[Ref.: #60090]	Geographic location (country and/or sea, region)	Kaohsiung County
[Ref.: #60090]	Country	Taiwan
[Ref.: #60090]	Country ISO 3 Code	TWN
[Ref.: #60090]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8235]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8235]	Azospirillum rugosum partial 16S rRNA gene, strain IMMIB AFH-6T	AM419042	1448	ENA	416170 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Azospirillum rugosum DSM 19657	2928256362	draft	JGI IMG/M	416170 tax ID	*

[Ref.: #32167]

GC-content

69-71 mol%

Availability in culture collections External links

[Ref.: #8235]

Culture collection no.

DSM 19657, CCUG 53966, IMMIB AFH-6

[Ref.: #83169]

SI-ID 398830

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Azospirillum thermophilum sp. nov., isolated from a hot spring.	Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003788	2020	*
Phylogeny	Azospirillum soli sp. nov., a nitrogen-fixing species isolated from agricultural soil.	Lin SY, Hameed A, Liu YC, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijsem.0.000618	2015	*
Phylogeny	Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.	Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.050872-0	2013	*
Phylogeny	Azospirillum formosense sp. nov., a diazotroph from agricultural soil.	Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.030585-0	2011	*
Phylogeny	Azospirillum rugosum sp. nov., isolated from oil-contaminated soil.	Young CC, Hupfer H, Siering C, Ho MJ, Arun AB, Lai WA, Rekha PD, Shen FT, Hung MH, Chen WM, Yassin AF	Int J Syst Evol Microbiol	10.1099/ijs.0.65065-0	2008	*

References

#8235

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19657

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32167

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28410 (see below)

#60090 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53966

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#83169

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398830.1 )

#28410

IJSEM 959 2008 ( DOI 10.1099/ijs.0.65065-0 , PubMed 18398202 )

* These data were automatically processed and therefore are not curated

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