Azospirillum rugosum (DSM 19657, CCUG 53966, IMMIB AFH-6)

Name: Azospirillum rugosum (DSM 19657, CCUG 53966, IMMIB AFH-6)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13998

Type strain

Culture col. no. DSM 19657 CCUG 53966 IMMIB AFH-6

NCBI tax ID(s) 416170

Special Collections DSMZ CCUG Literature strains

History <- IMMIB <- A. B. Arun, Dept. Soil and Environmental Sci., Natl. Chung Hsing Univ., Taichung, Taiwan; IMMIB AFH-6

Links

Azospirillum rugosum DSM 19657 is an aerobe, Gram-negative, motile bacterium that was isolated from oil-contaminated soil near the oil refinery located in Kaohsiung county.

Gram-negative motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398830

DSM 19657,CCUG 53966

@ref 20215

Last LPSN update 2026-02-09 11:21:17

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Azospirillaceae

Genus Azospirillum

Species Azospirillum rugosum

Full scientific name Azospirillum rugosum Young et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
32167	negative	rod-shaped

Pigmentation

32167

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8235	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
8235	positive	growth	28
32167	positive	growth	22-37
60090	positive	growth	35

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
32167	aerobe
60090	aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32167	NaCl	positive	growth	2 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32167	16808 ChEBI	2-dehydro-D-gluconate	+	carbon source
32167	17128 ChEBI	adipate	+	carbon source
32167	4853 ChEBI	esculin	+	hydrolysis
32167	28757 ChEBI	fructose	+	carbon source
32167	17754 ChEBI	glycerol	+	carbon source
32167	25115 ChEBI	malate	+	carbon source
32167	17632 ChEBI	nitrate	+	reduction
32167	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
32167	alkaline phosphatase	+	3.1.3.1
32167	alpha-galactosidase	+	3.2.1.22
32167	catalase	+	1.11.1.6
32167	cytochrome oxidase	+	1.9.3.1
32167	gelatinase	+
32167	pyrazinamidase	+	3.5.1.B15
32167	urease	+	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8235	oil-contaminated soil near the oil refinery located in Kaohsiung county		Taiwan, Province of China	TWN	Asia
60090	Oil contaminated soil	Kaohsiung County	Taiwan, Province of China	TWN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8235	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM1787615v1 assembly for Azospirillum rugosum IMMIB AFH-6	contig	GCA_017876155	416170.3	2918269953	416170	60.26
124043	ASM3081484v1 assembly for Azospirillum rugosum DSM 19657	contig	GCA_030814845	416170.5	2928256362	416170	60.25
124043	ASM4265514v1 assembly for Azospirillum rugosum CCUG 53966	scaffold	GCA_042655145			416170	31.1

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8235	Azospirillum rugosum partial 16S rRNA gene, strain IMMIB AFH-6T	AM419042	1448		416170

GC content

32167

GC-content (mol%)69-71

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR.	Stets MI, Alqueres SM, Souza EM, Pedrosa Fde O, Schmid M, Hartmann A, Cruz LM.	Appl Environ Microbiol	10.1128/aem.01351-15	2015
Genetics	Complete Genome Sequence of a Novel Azospirillum Strain TA Isolated from Western Siberia Chernevaya Taiga Soil.	Rayko M, Kravchenko I, Lapidus A.	Microorganisms	10.3390/microorganisms12122599	2024
	Glutamate wastewater as a culture medium for Azospirillum rugosum production and its impact on plant growth	Singh S, Rekha PD, Arun AB, Hamid A, Singh S, Shen FT, Young CC.	Biol Fertil Soils	10.1007/s00374-011-0547-3	2011
	Effect of mineral fertilizer, pig manure, and Azospirillum rugosum on growth and nutrient contents of Lactuca sativa L	Lai WA, Rekha PD, Arun AB, Young CC.	Biol Fertil Soils	10.1007/s00374-008-0313-3	2008
	Composted Cattle Manure Increases Microbial Activity and Soil Fertility More Than Composted Swine Manure in a Submerged Rice Paddy.	Das S, Jeong ST, Das S, Kim PJ.	Front Microbiol	10.3389/fmicb.2017.01702	2017
Metabolism	Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation.	Dashti N, Ali N, Salamah S, Khanafer M, Al-Shamy G, Al-Awadhi H, Radwan SS.	Microbiologyopen	10.1002/mbo3.630	2019
Phylogeny	Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil.	Lin SY, Liu YC, Hameed A, Hsu YH, Huang HI, Lai WA, Young CC.	Int J Syst Evol Microbiol	10.1099/ijsem.0.000904	2016
Phylogeny	Azospirillum thermophilum sp. nov., isolated from a hot spring.	Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003788	2020
Phylogeny	Azospirillum soli sp. nov., a nitrogen-fixing species isolated from agricultural soil.	Lin SY, Hameed A, Liu YC, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijsem.0.000618	2015
Phylogeny	Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.	Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.050872-0	2013
Phylogeny	Azospirillum formosense sp. nov., a diazotroph from agricultural soil.	Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.030585-0	2011
Phylogeny	Azospirillum rugosum sp. nov., isolated from oil-contaminated soil.	Young CC, Hupfer H, Siering C, Ho MJ, Arun AB, Lai WA, Rekha PD, Shen FT, Hung MH, Chen WM, Yassin AF	Int J Syst Evol Microbiol	10.1099/ijs.0.65065-0	2008

References

#8235	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19657
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28410	IJSEM 959 2008 ( DOI 10.1099/ijs.0.65065-0 , PubMed 18398202 )
#32167	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28410
#60090	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53966
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Azospirillum rugosum (DSM 19657, CCUG 53966, IMMIB AFH-6)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017876155

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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