Name: Pontibacillus chungwhensis BH030062
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1386

Type strain:

Species: Pontibacillus chungwhensis

Strain Designation: BH030062

Culture col. no.: DSM 16287

Strain history: <- C.-J. Kim; BH030062 <- J. Lim

NCBI tax ID(s): 265426 (species)

SI-ID 135137

DSM 16287, KCTC 3890

Special Collections

Section

Pontibacillus chungwhensis BH030062 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and has a yellow pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
moderately halophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Pontibacillus*
	Species	**Pontibacillus chungwhensis**
	Full Scientific Name (LPSN)	Pontibacillus chungwhensis Lim et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #23017]	Gram stain	positive

[Ref.: #23017]	Cell length	2.3-3.0 µm

[Ref.: #23017]	Cell width	0.6-0.9 µm

[Ref.: #23017]	Cell shape	rod-shaped

[Ref.: #23017]	Motility	yes

[Ref.: #23017]	Flagellum arrangement	peritrichous

[Ref.: #23017]	Colony color	yellow
[Ref.: #23017]	Colony shape	circular
[Ref.: #23017]	Incubation period	2 days
[Ref.: #23017]	Cultivation medium used	MA

[Ref.: #23017]	Pigment production	yes
[Ref.: #23017]	Pigmentation color	yellow

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6273]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6273]

Culture medium growth

[Ref.: #6273]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6273]

Culture medium composition

expand / minimize

[Ref.: #23017]

Culture medium

Marine agar (MA)

[Ref.: #23017]

Culture medium growth

		Temperature
[Ref.: #6273]	growth	30 °C
[Ref.: #23017]	growth	15.0-45.0 °C
[Ref.: #23017]	optimum	30.0-35.0 °C

	pH	Kind of pH		pH
[Ref.: #23017]			optimum	7.5-8.5
[Ref.: #23017]			growth	6.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23017]

Oxygen tolerance

obligate aerobe

[Ref.: #23017]	Spore description	spherical,terminal,in swollen sporangia
[Ref.: #23017]	Type of spore	endospore
[Ref.: #23017]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %95.605

[Ref.: #23017]

Halophily / tolerance level

moderately halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #23017]	NaCl		growth	1.0-15.0	%(w/v)
[Ref.: #23017]	NaCl		growth	2.0-5.0	%(w/v)
[Ref.: #23017]	NaCl	no	growth	>15.0	%(w/v)

[Ref.: #23017]	Murein short key	A31
[Ref.: #23017]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23017]	alpha-lactose ChEBI	-	builds acid from
[Ref.: #23017]	arbutin ChEBI	-	builds acid from
[Ref.: #23017]	casein	+	hydrolysis
[Ref.: #23017]	D-fructose ChEBI	-	builds acid from
[Ref.: #23017]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23017]	D-mannose ChEBI	-	builds acid from
[Ref.: #23017]	D-ribose ChEBI	+	builds acid from
[Ref.: #23017]	D-trehalose ChEBI	+	builds acid from
[Ref.: #23017]	D-xylose ChEBI	-	builds acid from
[Ref.: #23017]	esculin ChEBI	-	hydrolysis
[Ref.: #23017]	gelatin ChEBI	-	hydrolysis
[Ref.: #23017]	glucose ChEBI	+	builds acid from
[Ref.: #23017]	glycerol ChEBI	+	builds acid from
[Ref.: #23017]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #23017]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23017]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23017]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #23017]	maltose ChEBI	+	builds acid from
[Ref.: #23017]	melibiose ChEBI	-	builds acid from
[Ref.: #23017]	nitrate ChEBI	-	reduction
[Ref.: #23017]	raffinose ChEBI	-	builds acid from
[Ref.: #23017]	ribitol ChEBI	-	builds acid from
[Ref.: #23017]	salicin ChEBI	-	builds acid from
[Ref.: #23017]	starch ChEBI	+	hydrolysis
[Ref.: #23017]	tween 80 ChEBI	+	hydrolysis
[Ref.: #23017]	urea ChEBI	-	hydrolysis
[Ref.: #23017]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #23017]	catalase	+	1.11.1.6
[Ref.: #23017]	cytochrome oxidase	-	1.9.3.1
[Ref.: #23017]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6273]	Sample type/isolated from	solar saltern soil
[Ref.: #6273]	Country	Republic of Korea
[Ref.: #6273]	Country ISO 3 Code	KOR
[Ref.: #6273]	Continent	Asia

[Ref.: #23017]	Geographic location (country and/or sea, region)	Tae-An area on the Yellow Sea
[Ref.: #23017]	Enrichment culture	marine agar 2216 (MA; Difco) with the addition of 5 % (w/v) NaCl [final concentration: 6.94 % (w/v) NaCl]
[Ref.: #23017]	Enrichment culture duration	2 days
[Ref.: #23017]	Enrichment culture temperature	35.0 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY553296 (>99% sequence identity) for Pontibacillus from Microbeatlas

Aquatic Samples	25
Soil Samples	29
Animal Samples	40
Plant Samples	7
Total Samples	101

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6273]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23017]	Pontibacillus chungwhensis strain BH030062 16S ribosomal RNA gene, partial sequence	AY553296	1427	GenBank ENA	265426 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pontibacillus chungwhensis BH030062	GCA_000770675	contig	GenBank ENA	1385513 tax ID	*
[Ref.: #66792]	Pontibacillus chungwhensis BH030062	1385513.3	wgs	PATRIC	1385513 tax ID	*
[Ref.: #66792]	Pontibacillus chungwhensis BH030062	2627854003	draft	JGI IMG/M	1385513 tax ID	*

[Ref.: #23017]

GC-content

41.0 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pontibacillus chungwhensis BH030062 PATRIC: 1385513.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	82.514	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	89.272	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	95.605	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	76.027	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.545	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	83.844	no

Availability in culture collections External links

[Ref.: #6273]

Culture collection no.

DSM 16287

[Ref.: #71037]

SI-ID 135137

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pontibacillus salicampi sp. nov., a moderately halophilic bacterium isolated from saltern soil.	Lee JC, Kim YS, Yun BS, Whang KS	Int J Syst Evol Microbiol	10.1099/ijs.0.066423-0	2014	*
Phylogeny	Pontibacillus yanchengensis sp. nov., a moderately halophilic bacterium isolated from salt field soil.	Yang Y, Zou Z, He M, Wang G	Int J Syst Evol Microbiol	10.1099/ijs.0.023911-0	2010	*
Phylogeny	Pontibacillus litoralis sp. nov., a facultatively anaerobic bacterium isolated from a sea anemone, and emended description of the genus Pontibacillus.	Chen YG, Zhang YQ, Yi LB, Li ZY, Wang YX, Xiao HD, Chen QH, Cui XL, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.009910-0	2009	*
Phylogeny	Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin.	Chen YG, Zhang YQ, Xiao HD, Liu ZX, Yi LB, Shi JX, Zhi XY, Cui XL, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.002469-0	2009	*
Phylogeny	Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus.	Lim JM, Jeon CO, Park DJ, Kim HR, Yoon BJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63489-0	2005	*
Phylogeny	Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea.	Lim JM, Jeon CO, Song SM, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63315-0	2005	*

References

#6273

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16287

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23017

Jee-Min Lim,Che Ok Jeon,Sung Min Song,Chang-Jin Kim: Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. IJSEM 55: 165 - 170 2005 ( DOI 10.1099/ijs.0.63315-0 , PubMed 15653871 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71037

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID135137.1 )

* These data were automatically processed and therefore are not curated

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