Name: Bacillus tequilensis 10b
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 134164

Type strain:

Species: Bacillus tequilensis

Strain Designation: 10b

Culture col. no.: ATCC BAA-819, NCTC 13306, CIP 109396, NRRL B-41771, KCTC 13622

Strain history: CIP <- 2006, M.E. Hart, NCTR, Jefferson, Arkansas, USA: strain 10b

NCBI tax ID(s): 1423686 (strain), 227866 (species)

SI-ID 201847

ATCC BAA-819, NRRL B-41771, NCTC 13306, BCRC 17634, CCRC 17634, LMG 25326, CIP 109396

Special Collections

Other strains from Bacillus tequilensis

Section

Bacillus tequilensis 10b is an aerobe, spore-forming, Gram-positive bacterium that was isolated from Environment, Soil sample from a shaft tomb.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus tequilensis**
	Full Scientific Name (LPSN)	Bacillus tequilensis Gatson et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31639]	Gram stain	positive
[Ref.: #123444]	Gram stain	positive

[Ref.: #31639]	Cell length	4 µm

[Ref.: #31639]	Cell width	0.9 µm

[Ref.: #31639]	Cell shape	rod-shaped
[Ref.: #123444]	Cell shape	rod-shaped

[Ref.: #31639]	Motility	yes
[Ref.: #123444]	Motility	yes

[Ref.: #31639]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #37000]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37000]

Culture medium growth

[Ref.: #37000]

Culture medium composition

expand / minimize

[Ref.: #123444]

Culture medium

CIP Medium 72

[Ref.: #123444]

Culture medium growth

[Ref.: #123444]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #31639]	growth	25-50 °C
[Ref.: #31639]	optimum	40-50 °C
[Ref.: #37000]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #31639]			optimum	6.5-8
[Ref.: #31639]			growth	5.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31639]	Oxygen tolerance	aerobe
[Ref.: #123444]	Oxygen tolerance	obligate aerobe

[Ref.: #31639]	Ability of spore formation	yes
[Ref.: #123444]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %91.599

[Ref.: #31639]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	fructose ChEBI	+	carbon source
[Ref.: #31639]	fucose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	glucose ChEBI	+	carbon source
[Ref.: #31639]	glycerol ChEBI	+	carbon source
[Ref.: #31639]	glycogen ChEBI	+	carbon source
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	L-arabitol ChEBI	+	carbon source
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	lactose ChEBI	+	carbon source
[Ref.: #31639]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	melibiose ChEBI	+	carbon source
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #123444]	nitrate ChEBI	+	reduction
[Ref.: #123444]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	raffinose ChEBI	+	carbon source
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	rhamnose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	salicin ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	sorbitol ChEBI	+	carbon source
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31639]	xylitol ChEBI	+	carbon source
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31639]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #31639]		indole ChEBI	yes
[Ref.: #123444]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #31639]		indole ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31639]	catalase	+	1.11.1.6
[Ref.: #123444]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31639]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #31639]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123444]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #123444]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #123444]	11547	not determinedn.d.	+/-	-	-	+	+	+	-	-	-	+/-	+	+	-	-	-	-	-	+	-	-	+/-	+/-	+	+	+	+	+	+	+/-	-	+	+	+	-	-	+	+	-	+	+/-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123444]	11838	-	+	+	+	-	+	-	-	+	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #123444]	Sample type/isolated from	Environment, Soil sample from a shaft tomb
[Ref.: #123444]	Geographic location (country and/or sea, region)	Huitzilapa, near Tequila
[Ref.: #123444]	Country	Mexico
[Ref.: #123444]	Country ISO 3 Code	MEX
[Ref.: #123444]	Continent	North America
[Ref.: #123444]	Isolation date	1999
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #123444]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31639]	Bacillus tequilensis strain 10b 16S ribosomal RNA gene, partial sequence	AY197613	549	GenBank ENA	227866 tax ID
[Ref.: #124043]	Bacillus tequilensis strain KCTC 13622 16S ribosomal RNA gene, partial sequence.	OQ876678	1448	GenBank ENA	227866 tax ID	*
[Ref.: #124043]	Proteus sp. BAB-5300 16S ribosomal RNA gene, partial sequence.	KT262934	1091	GenBank ENA	227866 tax ID	*
[Ref.: #124043]	Bacillus tequilensis strain KCTC 13622 16S ribosomal RNA gene, partial sequence.	OR421517	1401	GenBank ENA	227866 tax ID	*
[Ref.: #124043]	Bacillus tequilensis strain KCTC 13622(T) 16S ribosomal RNA gene, partial sequence.	MZ669863	789	GenBank ENA	227866 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1005	LS998021	901	GenBank ENA	1423686 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1012	LS998694	904	GenBank ENA	1423686 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1001	LS998695	935	GenBank ENA	1423686 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1014	LS998697	850	GenBank ENA	1423686 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1015	LS998698	888	GenBank ENA	1423686 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1016	LS998699	887	GenBank ENA	1423686 tax ID	*
[Ref.: #124043]	Bacillus tequilensis KCTC 13622 partial 16S rRNA gene, strain JC1017	LS998700	910	GenBank ENA	1423686 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus tequilensis NCTC13306	GCA_900445435	contig	GenBank ENA	227866 tax ID	*
[Ref.: #66792]	Bacillus tequilensis strain NCTC13306	227866.8	wgs	PATRIC	227866 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus tequilensis strain NCTC13306 PATRIC: 227866.8
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	77.797	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	90.002	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	91.599	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	66.192	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.413	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	78.422	no

Availability in culture collections External links

[Ref.: #31639]

Culture collection no.

ATCC BAA-819, NCTC 13306, CIP 109396, NRRL B-41771, KCTC 13622

[Ref.: #91762]

SI-ID 201847

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis.	Gatson JW, Benz BF, Chandrasekaran C, Satomi M, Venkateswaran K, Hart ME	Int J Syst Evol Microbiol	10.1099/ijs.0.63946-0	2006	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31639

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27922 (see below)

#37000 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#91762

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID201847.1 )

#123444 Collection of Institut Pasteur ; Curators of the CIP; CIP 109396

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#27922

IJSEM 1475 2006 ( DOI 10.1099/ijs.0.63946-0 , PubMed 16825615 )

* These data were automatically processed and therefore are not curated

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Bacillus tequilensis strain NCTC13306

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