Strain identifier

BacDive ID: 134164

Type strain: Yes

Species: Bacillus tequilensis

Strain Designation: 10b

Strain history: CIP <- 2006, M.E. Hart, NCTR, Jefferson, Arkansas, USA: strain 10b

NCBI tax ID(s): 227866 (species)

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General

@ref: 31639

BacDive-ID: 134164

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Bacillus tequilensis 10b is an aerobe, spore-forming, Gram-positive bacterium that was isolated from Environment, Soil sample from a shaft tomb.

NCBI tax id

  • NCBI tax id: 227866
  • Matching level: species

strain history

@refhistory
370002006, M.E. Hart, NCTR, Jefferson, Arkansas, USA: strain 10b
123444CIP <- 2006, M.E. Hart, NCTR, Jefferson, Arkansas, USA: strain 10b

doi: 10.13145/bacdive134164.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus tequilensis
  • full scientific name: Bacillus tequilensis Gatson et al. 2006

@ref: 31639

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus tequilensis

strain designation: 10b

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31639positive4 µm0.9 µmrod-shapedyes
123444positiverod-shapedyes

pigmentation

  • @ref: 31639
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37000MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123444CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
31639positivegrowth25-50
31639positiveoptimum40-50thermophilic
37000positivegrowth30mesophilic

culture pH

@refabilitytypepH
31639positivegrowth5.5-8
31639positiveoptimum6.5-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31639aerobe
123444obligate aerobe

spore formation

@refspore formation
31639yes
123444yes

observation

  • @ref: 31639
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3163922599arabinose+carbon source
3163918403L-arabitol+carbon source
3163917057cellobiose+carbon source
3163928757fructose+carbon source
3163933984fucose+carbon source
3163928260galactose+carbon source
3163917234glucose+carbon source
3163917754glycerol+carbon source
3163928087glycogen+carbon source
3163917716lactose+carbon source
3163917306maltose+carbon source
3163928053melibiose+carbon source
31639506227N-acetylglucosamine+carbon source
3163916634raffinose+carbon source
3163926546rhamnose+carbon source
3163917814salicin+carbon source
3163930911sorbitol+carbon source
3163917992sucrose+carbon source
3163927082trehalose+carbon source
3163917151xylitol+carbon source
3163918222xylose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin+builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
12344417632nitrate+reduction
12344416301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
3163935581indoleyes
12344435581indoleno

metabolite tests

  • @ref: 31639
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
31639catalase+1.11.1.6
31639gelatinase+
31639cytochrome oxidase+1.9.3.1
123444oxidase+
123444catalase+1.11.1.6
123444urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123444-+++-+--+-++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123444+/---+++---+/-++-----+--+/-+/-+++++++/--+++--++-++/----------

Isolation, sampling and environmental information

isolation

  • @ref: 123444
  • sample type: Environment, Soil sample from a shaft tomb
  • geographic location: Huitzilapa, near Tequila
  • country: Mexico
  • origin.country: MEX
  • continent: North America
  • isolation date: 1999

Safety information

risk assessment

  • @ref: 123444
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 31639
  • description: Bacillus tequilensis strain 10b 16S ribosomal RNA gene, partial sequence
  • accession: AY197613
  • length: 549
  • database: nuccore
  • NCBI tax ID: 227866

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus tequilensis NCTC13306GCA_900445435contigncbi227866
66792Bacillus tequilensis strain NCTC13306227866.8wgspatric227866

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes77.659no
gram-positiveyes89.092yes
anaerobicno99.148yes
aerobicyes89.576yes
halophileyes89.559no
spore-formingyes91.673yes
glucose-utilyes88.391yes
flagellatedyes73.092no
thermophileno97.73no
glucose-fermentno87.707no

External links

@ref: 31639

culture collection no.: ATCC BAA 819, NCTC 13306, CIP 109396, NRRL B-41771, KCTC 13622

straininfo link

  • @ref: 91762
  • straininfo: 201847

literature

  • topic: Phylogeny
  • Pubmed-ID: 16825615
  • title: Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis.
  • authors: Gatson JW, Benz BF, Chandrasekaran C, Satomi M, Venkateswaran K, Hart ME
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63946-0
  • year: 2006
  • mesh: Archaeology, Bacillus/chemistry/*classification/*isolation & purification/physiology, Bacterial Proteins/genetics, Bacterial Typing Techniques, Burial, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Gel, Pulsed-Field, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Mexico, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Superoxide Dismutase/genetics
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlID_cross_referencepubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
31639Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172792228776041
37000Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7133
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
91762Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID201847.1
123444Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109396Collection of Institut Pasteur (CIP 109396)