Name: Enterococcus alcedinis L34
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133990

Type strain:

Species: Enterococcus alcedinis

Strain Designation: L34

Culture col. no.: CCM 8433, LMG 27164, DSM 107709

Strain history: <- CCM; CCM 8433 <- P. Frolkova; L34

NCBI tax ID(s): 1274384 (species)

SI-ID 381134

LMG 27164, CCM 8433, DSM 107709

Section

Enterococcus alcedinis L34 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from kingfisher.

coccus-shaped
Gram-positive
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus alcedinis**
	Full Scientific Name (LPSN)	Enterococcus alcedinis Frolková et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #31015]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #31015]	Cell length	1.5 µm

[Ref.: #31015]	Cell width	1.5 µm

[Ref.: #31015]	Cell shape	coccus-shaped

[Ref.: #31015]	Motility	no

[Ref.: #31015]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64748]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #64748]

Culture medium growth

[Ref.: #64748]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64748]

Culture medium composition

expand / minimize

[Ref.: #64748]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #64748]

Culture medium growth

[Ref.: #64748]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64748]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #31015]			growth	15-42 °C
[Ref.: #64748]			growth	37 °C

[Ref.: #64748]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31015]

Oxygen tolerance

facultative anaerobe

[Ref.: #31015]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.792

[Ref.: #31015]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	arginine ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31015]	dextrin ChEBI	+	carbon source
[Ref.: #31015]	esculin ChEBI	+	hydrolysis
[Ref.: #31015]	fructose ChEBI	+	carbon source
[Ref.: #31015]	galactose ChEBI	+	carbon source
[Ref.: #31015]	glucose ChEBI	+	carbon source
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #31015]	inosine ChEBI	+	carbon source
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #31015]	mannitol ChEBI	+	carbon source
[Ref.: #31015]	mannose ChEBI	+	carbon source
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31015]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31015]	salicin ChEBI	+	carbon source
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31015]	sucrose ChEBI	+	carbon source
[Ref.: #68381]	sucrose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31015]	thymidine ChEBI	+	carbon source
[Ref.: #31015]	trehalose ChEBI	+	carbon source
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #31015]	uridine ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	no	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	-	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #31015]	acid phosphatase	+	3.1.3.2
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	+	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	-	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #64748]	10782	-	+/-	+/-	-	-	+	+	+/-	-	-	+	-	-	-	-	not determinedn.d.	-	-	+	+	-	+	-	-	-	+	-	+/-	+/-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #31015]	Sample type/isolated from	kingfisher

[Ref.: #64748]	Sample type/isolated from	Cloaca of kingfisher Alcedo atthis
[Ref.: #64748]	Geographic location (country and/or sea, region)	Kacov, Vlasim
[Ref.: #64748]	Country	Czech Republic
[Ref.: #64748]	Country ISO 3 Code	CZE
[Ref.: #64748]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host

#Birds

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64748]	Enterococcus alcedinis strain L34 16S ribosomal RNA gene, partial sequence	JX948102	1260	ENA	1274384 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus alcedinis CCM 8433	GCA_014635985	contig	GenBank	1274384 tax ID	*
[Ref.: #66792]	Enterococcus alcedinis DSM 107709	GCA_017874975	contig	GenBank	1274384 tax ID	*
[Ref.: #66792]	Enterococcus alcedinis strain CCM 8433	1274384.3	wgs	PATRIC	1274384 tax ID	*
[Ref.: #66792]	Enterococcus alcedinis strain DSM 107709	1274384.4	wgs	PATRIC	1274384 tax ID	*
[Ref.: #66792]	Enterococcus alcedinis DSM 107709	2913599697	draft	JGI IMG/M	1274384 tax ID	*

[Ref.: #31015]	GC-content	35.1 mol%
[Ref.: #64748]	GC-content	35.1 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus alcedinis strain DSM 107709 PATRIC: 1274384.4
[Ref.: #69481]	spore-forming	no	71	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus alcedinis strain CCM 8433 PATRIC: 1274384.3
[Ref.: #69480]	motile	no	63.644	yes
[Ref.: #69480]	gram-positive	yes	91.927	yes
[Ref.: #69480]	anaerobic	no	97.432	no
[Ref.: #69480]	halophile	yes	72.089	no
[Ref.: #69480]	spore-forming	no	71.916	no
[Ref.: #69480]	thermophile	no	99.505	no
[Ref.: #69480]	glucose-util	yes	87.027	yes
[Ref.: #69480]	flagellated	no	86.393	yes
[Ref.: #69480]	aerobic	no	94.269	yes
[Ref.: #69480]	glucose-ferment	yes	80.504	no

Availability in culture collections External links

[Ref.: #64748]

Culture collection no.

CCM 8433, LMG 27164, DSM 107709

[Ref.: #91658]

SI-ID 381134

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Enterococcus alcedinis sp. nov., isolated from common kingfisher (Alcedo atthis).	Frolkova P, Svec P, Sedlacek I, Maslanova I, Cernohlavkova J, Ghosh A, Zurek L, Radimersky T, Literak I	Int J Syst Evol Microbiol	10.1099/ijs.0.049833-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31015

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27345 (see below)

#64748

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107709

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91658

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID381134.1 )

#27345

IJSEM 3069 2013 ( DOI 10.1099/ijs.0.049833-0 , PubMed 23416573 )

* These data were automatically processed and therefore are not curated

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Enterococcus alcedinis strain DSM 107709

Enterococcus alcedinis strain CCM 8433

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